• Journal of Life Science
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• v.26 no.12
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• pp.1376-1382
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• 2016

Xylitol is widely used in the food and medical industry. It is produced by the reduction of xylose (lignocellulosic biomass) in the Saccharomyces cerevisiae strain, which is considered genetically safe. In this study, the expression system of the GRE3 (YHR104W) gene that encodes xylose reductase was constructed to efficiently produce xylitol in the S. cerevisiae strain, and the secretory production of xylose reductase was investigated. To select a suitable promoter for the expression of the GRE3 gene, pGMF-GRE3 and pAMF-GRE3 plasmid with GAL10 promoter and ADH1 promoter, respectively, were constructed. The mating factor ${\alpha}$ ($MF{\alpha}$) signal sequence was also connected to each promoter for secretory production. Each plasmid was transformed into S. cerevisiae $SEY2102{\Delta}trp1$, and $SEY2102{\Delta}trp1$/pGMF- GRE3 and $SEY2102{\Delta}trp1$/pAMF-GRE3 transformants were selected. In the $SEY2102{\Delta}trp1$/pGMF-GRE3 strain, the total activity of xylose reductase reached 0.34 unit/mg-protein when NADPH was used as a cofactor; this activity was 1.5 fold higher than that in $SEY2102{\Delta}trp1$/pAMF-GRE3 with ADH1 as the promoter. The secretion efficiency was 91% in both strains, indicating that most of the recombinant xylose reductase was efficiently secreted in the extracellular fraction. In a baffled flask culture of the $SEY2102{\Delta}trp1$/pGMF-GRE3 strain, 12.1 g/l of xylitol was produced from 20 g/l of xylose, and ~83% of the consumed xylose was reduced to xylitol.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.33 no.3
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• pp.191-198
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• 2007

The p53 which is well known as tumor suppressor gene is located at 17p13. p53 is a sequence-specific DNA binding transcription factor that responds to certain cytotoxic stresses, such as DNA damage, by enhancing the transcription of genes that regulate cell-cycle progression as well as programmed cell death. The p63 gene that is located at 3q27-29, is recognized members of the p53 family, and responsible for the transcription of 6 isoforms. Three isoforms ($TAp63{\alpha}$, $TAp63{\beta}$, $TAp63{\gamma}$) contain an N-terminal transactivation (TA) domain and can induce apoptosis. The other 3 isoforms (${\Delta}Np63{\alpha}$, ${\Delta}Np63{\beta}$, ${\Delta}Np63{\gamma}$) lack the TA domain and may function in a dominant-negative fashion by inhibiting the transactivation functions of p53 and TAp63 proteins, and thus act as oncoproteins. A number of studies have investigated the role of p63 in human squamous cell carcinomas from different organs. Only a few studies have examined ${\Delta}Np63$ isoform in oral squamous cell carcinoma including normal epithelium. This study aimed to evaluate expression of ${\Delta}Np63$ isoform in human oral squamous cell carcinoma tissue and normal mucosa. The 3 cases of well differenciated oral squamous cell carcinoma specimen including adjacent normal mucosa were examined, and immunohistochemical study with monoclonal antibody(4A4) and tumor cell apoptosis analysis with Transmission Electon Microscopy were studied. And, RT-PCR analysis was done for expression of ${\Delta}Np63$ isoform. The results were as followed. 1. Normal gingiva showed the restricted p63 expression in basal cell layer. 2. Well differentiated squamous cell carcinoma showed mainly p63 expression in overall area of malignancy, especially in basal cell layer to adjacent stromal tissue. 3. Tumor cells around keratinized area with no p63 expression disclosed less micro-organelle in decreased size cytoplasm and severe chromatin margination with nuclear destruction that means apoptosis. 4. Comparison of mRNA expression of ${\Delta}Np63$ isoform by RT-PCR showed variable expression of ${\Delta}Np63$ isoform, but ${\Delta}Np63{\alpha}$ was most highly expressed in all 3 tumor specimen. From theses results, it should be suggested that ${\Delta}Np63$ isoform expression in well differentiated squamous cell carcinoma was closely related to tumor oncogenesis, expecially overexpression of ${\Delta}Np63{\alpha}$ is a most important factor in tumor genesis of oral squamous cell carcinoma.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.4
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• pp.623-631
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• 2003

It is considered that the application of advanced composite materials to the prostheses for the disables is important to improve their bio-mechanical performance. Particularly, energy storing foot prosthesis is mostly important to restore gait ability of the disables with low-extremity amputation since it could provide propulsion at terminal stance enhancing the disables ability to walk long distance even run and jump. Therefore, the energy storing spring of Prosthetic foot keel under cyclic bending moment use mainly of high strength glass fiber reinforced plastic. The main objective of this study was to evaluate the stacking sequence effect using the delamination growth rate(dA$_{D}$/dN) of energy storing spring in glass fiber reinforced plastic under cyclic bending moment. The test results indicated that the shape of delamination zone depends on stacking sequence in GFRP laminates. Delamination area(A$_{D}$) turns out that variable types with the contour increased non-linearly toward the damage zones.nes.

• The Transactions of the Korean Institute of Power Electronics
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• v.20 no.4
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• pp.321-329
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• 2015

The static synchronous compensator (STATCOM) of cascaded H-bridge configuration accompanying multiple separate DC sides is inherently subject to the problem of uneven DC voltages. These DC voltages in one leg can be controlled by adjusting the AC-side output voltage of each cell inverter, which is proportional to the active power. However, when the phase current is extremely small, large AC-side voltage is required to generate the active power to balance the cell voltages. In this study, an alternative zero-sequence current injection method is proposed, which facilitates effective cell balancing controllers at no load, and has no effect on the power grid because the injected zero sequence current only flows within the STATCOM delta circuit. The performance of the proposed method is verified through simulation and experiments.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.12
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• pp.2963-2967
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• 2015

The pump-probe scheme is used to analyze the cross-phase modulation penalty of a single-mode fiber in a WDM system. The pump signal is assumed to be a periodically modulated input like a raised sinusoidal. The periodic signal models an alternating bit sequence, and leads to an analytical expression of CPM penalty which is measured by EOP. The derived expression shows good agreement with numerical results in conventional single-mode fiber systems over a wide range of channel spacing, ${\Delta}f$. In dispersion-shifted fiber systems when ${\Delta}f$ < 100GHz, the derived expression shows increased discrepancy with the numerical results due to the increased FWM. This is not a surprising because the pump-probe scheme is used to analyze system performance degradation due to CPM.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.874-880
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• 2010

A series of 13- and 14-membered cyclic enkephalin analogs based on the moderately $\mu$ selective prototype compound Tyr-C[D-$A_2bu$-Gly-Phe-Leu] 8a were synthesized to investigate the structure-activity relationship. The modifications of sequence were mainly focused on two positions 3 and 5, critical for the selective recognition for $\mu$ and $\delta$ opioid receptors. The substitution of hydrophobic $Leu^5$ with hydrophilic $Asp^5$ derivatives led to Tyr-C[D-$A_2bu$-Gly-Phe-Asp(N-Me)] 7 and Tyr-C[D-Glu-Phe-gPhe-rAsp(O-Me)] 5, the peptides with a large affinity losses at both $\mu$ and $\delta$ receptors. The substitution of $Phe^3$ with $Gly^3$ led to Tyr-C[D-Glu-Gly-gPhe-rLeu] 3 and Tyr-C[D-Glu-Gly-gPhe-D-rLeu] 4, the peptides with large affinity losses at $\mu$ receptors, indicating the critical role of phenyl ring of $Phe^3$ for $\mu$ receptor affinities. One atom reduction of the ring size from 14-membered analogs Tyr-C[D-Glu-Phe-gPhe-(L and D)-rLeu] 6a, 6b to 13-membered analogs Tyr-C[D-Asp-Phe-gPhe-(L and D)-rLeu] 1, 2 reduced the affinity at both $\mu$ and $\delta$ receptors, but increased the potency in the nociceptive assay, indicating the ring constrain is attributed to high nociceptive potency of the analogs. For the influence of D- or L-chirality of $Leu^5$ on the receptor selectivity, regardless of chirality and ring size, all cyclic diastereomers displayed marked $\mu$ selectivity with low potencies at the $\delta$ receptor. The retro-inverso analogs display similar or more active at $\mu$ receptor, but less active at $\delta$ receptor than the parent analogs.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.1
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• pp.28-32
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• 2009

We describe here the cloning and characterization of a cDNA encoding the glutathione S-transferase (GST) from the bumblebee Bombus ignitus. The Delta-class B. ignitus GST (BiGSTD) gene spans 1668 bp and consists of four introns and five exons that encode 216 amino acid residues with a calculated molecular weight of approximately 24561 Da and a pI of 8.03. The N-terminal domain of BiGSTD has a conserved Ser residue, as well as conserved Lys, Pro, Glu, Ser and Tyr residues that are involved in the GSH-binding site of GST. The BiGSTD showed 60% protein sequence identity to the Bombyx mori GSTT1, 58% to Musca domestica GST, 57% to Drosophila melanogaster GST, and 55% to Anopheles gambiae GST1. BiGSTD was close to the insect-specific Delta class of GSTs in a phylogenetic tree. Northern blot analysis showed that BiGSTD is highly expressed in the fat body and midgut, and less so in the muscles of B. ignitus worker bees.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1413-1421
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• 2013

The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, $C20:5{\omega}-3$) and docosahexaenoic acid (DHA, $C22:6{\omega}-3$) that are important to human health. Here, we report a functional characterization of a ${\Delta}4$-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.

• East Asian mathematical journal
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• v.24 no.1
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• pp.35-43
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• 2008

Let E be a uniformly convex Banach space and K be a nonempty closed convex subset of E. Let ${\{T_i\}}^N_{i=1}$ be N nonexpansive self-mappings of K with $F\;=\;{\cap}^N_{i=1}F(T_i)\;{\neq}\;{\theta}$ (here $F(T_i)$ denotes the set of fixed points of $T_i$). Suppose that one of the mappings in ${\{T_i\}}^N_{i=1}$ is semi-compact. Let $\{{\alpha}_n\}\;{\subset}\;[{\delta},\;1-{\delta}]$ for some ${\delta}\;{\in}\;(0,\;1)$ and $\{{\beta}_n\}\;{\subset}\;[\tau,\;1]$ for some ${\tau}\;{\in}\;(0,\;1]$. For arbitrary $x_0\;{\in}\;K$, let the sequence {$x_n$} be defined iteratively by $\{{x_n\;=\;{\alpha}_nx_{n-1}\;+\;(1-{\alpha}_n)T_ny_n,\;\;\;\;\;\;\;\;\; \atop {y_n\;=\;{\beta}nx_{n-1}\;+\;(1-{\beta}_n)T_nx_n},\;{\forall}_n{\geq}1,}$, where $T_n\;=\;T_{n(modN)}$. Then {$x_n$} convergence strongly to a common fixed point of the mappings family ${\{T_i\}}^N_{i=1}$. The result presented in this paper generalized and improve the corresponding results of Chidume and Shahzad [C. E. Chidume, N. Shahzad, Strong convergence of an implicit iteration process for a finite family of nonexpansive mappings, Nonlinear Anal. 62(2005), 1149-1156] even in the case of ${\beta}_n\;{\equiv}\;1$ or N=1 are also new.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.586-589
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• 2003

Previously we reported the cloning and sequence analysis of a CFTase gene from Bacillus polymyxa. CFTase was divided into five distinct regions. In order to understand a role of the N-terminal repeat region on the function of CFTase from Bacillus polymyxa MGL21, deletion mutantCFTase ${\Delta}N$ was prepered. Recombinant protein was overproduced in E. coli as inclusion body, solubilized in bufer containing 8M urea, and refolded in the phosphate buffer. The molecular weight of the purified wild type CFTase and CFTase ${\Delta}N$ were 148kDa , 108kDa, respectively.