• Journal of the Korean Society for Marine Environment & Energy
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• v.14 no.2
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• pp.121-129
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• 2011

The EFDC model has been simplified to enhance the computing performance in hydrodynamic modeling. Water quality module and unnecessary conditional statements were deleted in subroutine list and memory allocation. The performance of the enhanced model (EFDC-E) was checked by applying EFDC and EFDC-E models to simulating the tidal flow in Mokpo coastal zone. Both two-dimensional models and threedimensional models have been applied and compared. Three-dimensional models showed better simulation results agreeing with observed currents than two-dimensional models. The simulation results of EFDC-E model gave good results agreeing with the simulation results of EFDC model and the observed data. The computing speed of EFDC-E model is improved 3 times faster than that of EFDC model in modeling hydrodynamic flow for real time of 3 days in both 2-dimensional modeling and 3-dimensional modeling. The EFDC-E model can be used widely for hydrodynamic modeling because of improved simulation speed.

• Journal of the Society of Disaster Information
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• v.10 no.3
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• pp.408-418
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• 2014

This study is to clarify the effect of security majoring students' loyalty on department to future direction and job choice to suggest basic data for supporting student to prepare more active school life and future. The research object is security majoring male/female students among Y university in Gyeonggi-do Y city, K university in Gyeongsangbuk-do G city, k university in Gyeonggi-do S city between 1 Fab 2012 to 20 Aug 2012 and we randomly extracted the sample. In this research, we performed exploratory factor analysis and used principal components analysis and selected varimax direct rotation method to prove the reasonability of the contents. We then use the Cronbach's ${\alpha}$ value to measure the trust coefficient and during the process, the repeated or questions lack of correlation were deleted with judgment of researcher to improve trusty and reasonability.

• Molecules and Cells
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• v.24 no.2
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• pp.261-267
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• 2007

Apoptotic signals are typically accompanied by activation of aspartate-specific cysteine proteases called caspases, and caspase-3 and -7 play crucial roles in the execution of apoptosis. Previously, using the proteomic approach, protein disulfide isomerase (PDI) was found to be a candidate substrate of caspase-7. This abundant 55 kDa protein introduces disulfide bonds into proteins (via its oxidase activity) and catalyzes the rearrangement of incorrect disulfide bonds (via its isomerase activity). PDI is abundant in the ER but is also found in non-ER locations. In this study we demonstrated that PDI is cleaved by caspase-3 and -7 in vitro. In addition, in vivo experiment showed that it is cleaved during etoposide-induced apoptosis in HL-60 cells. Subcellular fractionation showed that PDI was also present in the cytosol. Furthermore, only cytosolic PDI was clearly digested by caspase-3 and -7. It was also confirmed by confocal image analysis that PDI and caspase-7 partially co-localize in both resting and apoptotic MCF-7 cells. Overexpression of cytosolic PDI (ER retention sequence deleted) inhibited cell death after an apoptotic stimulus. These data indicate that cytosolic PDI is a substrate of caspase-3 and -7, and that it has an anti-apoptotic action.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.4
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• pp.82-88
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• 2009

Video proxy server which is located near clients can store the frequently requested video data in storage space in order to minimize initial latency and network traffic significantly. However, due to the limited storage space in video proxy server, an appropriate video selection method is needed to store the videos which are frequently requested by users. Thus, we present a virtual caching technique to efficiently store the video in video proxy server. For this purpose, we employ a virtual memory in video poky server. If the video is requested by user, it is loaded in virtual memory first and then, delivered to the user. A video which is loaded in virtual memory is deleted or moved into the storage space of video poxy sewer depending on the request condition. In addition, virtual memory is divided into each segment area in order to store the segments efficiently and to avoid the fragmentation. The simulation results show that the proposed method performs better than other methods in terms of the block hit rate and the number of block deletion.

• Phonetics and Speech Sciences
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• v.7 no.3
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• pp.45-53
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• 2015

The current study investigated CVCC syllables in spontaneous American English speech to find out whether such syllables are produced as phonological units with a string of segments, showing a hierarchical structure. Transcribed data from the Buckeye Speech Corpus was used for the analysis in this study. The result of the current study showed that the constituents within a CVCC syllable as a phonological unit may have phonetic variations (namely, the final coda may undergo deletion). First, voiceless alveolar stops were the most frequently deleted when they occurred as the second final coda consonants of a CVCC syllable; this deletion may be an intermediate process on the way from the abstract form CVCC (with the rime VCC) to the actual pronunciation CVC (with the rime VC), a production strategy employed by some individual speakers. Second, in the internal structure of the rime, the proportion of deletion of the final coda consonant depended on the frequency of the word rather than on the position of postvocalic consonants on the sonority hierarchy. Finally, the segment following the consonant cluster proved to have an effect on the reduction of that cluster; more precisely, the following contrast was observed between obstruents and non-obstruents, reflecting the effect of sonority: when the segment following the consonant cluster was an obstruent, the proportion of deletion of the final coda consonant was increased. Among these results, the effect of word frequency played a critical role for promoting the deletion of the second coda consonant for clusters in CVCC syllables in spontaneous speech. The current study implies that the structure of syllables as phonological units can vary depending on individual speakers' lexical representation.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.5
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• pp.225-230
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• 2008

Netrins are secreted molecules and involved in axon guidance, cell migration and tumor development. Recent studies revealed that netrins perform novel functions in such processes as epithelial development and angiogenesis without operating through the classical netrin receptors, DCC (Deleted in Colorectal Cancer) and Unc5h. In the present study, we investigated the roles of netrin-1 and its receptors in cell spreading of human glioblastoma cells, and found that netrin-1 haptotactically enhanced fibronectin-induced cell spreading and focal adhesion formation in U373 glioblastoma cells. Netrin-1 binding to the U373 cell membrane was blocked by an antibody against ${\alpha}v$ integrin subunit, but not by an anti-DCC or anti-Unc5h antibody. In addition, enhancement of the fibronectin response by netrin-1 was abrogated by a function blocking antibody against integrin ${\alpha}v{\beta}3$. Since the ${\alpha}v$ subunit of the integrin family plays an important role in the pathophysiological aspects of cell migration, including tumor angiogenesis and metastasis, our data provide important insight into the molecular mechanism of netrin function.

• 한국노년학
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• v.30 no.1
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• pp.65-80
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• 2010

Purpose: The purpose of this study was to develop a wisdom scale among Korean elderly with high validity and reliability. Method: The process of the development of this scale follows: A conceptual framework was identified based on literature a review of the elderly and discussion with experts in physiology and sociology. Total 128 items were developed. Through reliability testing, factor analysis, 60 preliminary items, and 4-points scale were selected. By a means of the internal consistency for 60 items, 33 items were deleted. Finally, 27 items were remained. To verity 27 items, factor analysis, reliability testing, and correlation were done. Data were collected from 480 elderly subjects in Daegu, Kyungpook Province, and Busan, Kyungnam Province from August, 2008 to February, 2009. Results: From the results of the factor analysis for 27 items, 3 factors were extracted. These factors were labeled as 'empathic emotion', 'self-reflection', and 'bitter experience of life'. Cronbach's Alpha for the 27 items was .921 and correlation coefficient of ego-integrity was .69. Conclusion: An exploratory study of the variables related to wisdom among the elderly is needed for criterion validity of this scale. A study on wisdom for different age groups is needed for re-verification.

• BMB Reports
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• v.52 no.2
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• pp.133-138
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• 2019

Upon viral infection, the 2', 5'-oligoadenylate synthetase (OAS)-ribonuclease L (RNaseL) system works to cleave viral RNA, thereby blocking viral replication. However, it is unclear whether OAS proteins have a role in regulating gene expression. Here, we show that OAS1 and OAS3 act as negative regulators of the expression of chemokines and interferon-responsive genes in human macrophages. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) technology was used to engineer human myeloid cell lines in which the OAS1 or OAS3 gene was deleted. Neither OAS1 nor OAS3 was exclusively responsible for the degradation of rRNA in macrophages stimulated with poly(I:C), a synthetic surrogate for viral double-stranded (ds)RNA. An mRNA sequencing analysis revealed that genes related to type I interferon signaling and chemokine activity were increased in $OAS1^{-/-}$ and $OAS3^{-/-}$ macrophages treated with intracellular poly(I:C). Indeed, retinoic-acid-inducible gene (RIG)-I- and interferon-induced helicase C domain-containing protein (IFIH1 or MDA5)-mediated induction of chemokines and interferon-stimulated genes was regulated by OAS3, but Toll-like receptor 3 (TLR3)- and TLR4-mediated induction of those genes was modulated by OAS1 in macrophages. However, stimulation of these cells with type I interferons had no effect on OAS1- or OAS3-mediated chemokine secretion. These data suggest that OAS1 and OAS3 negatively regulate the expression of chemokines and interferon-responsive genes in human macrophages.

• BMB Reports
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• v.54 no.8
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• pp.419-424
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• 2021

Cold-induced norepinephrine activates β3-adrenergic receptors (β3-AR) to stimulate the kinase cascade and cAMP-response element-binding protein, leading to the induction of thermogenic gene expression including uncoupling protein 1 (Ucp1). Here, we showed that stimulation of the β3-AR by its agonists isoproterenol and CL316,243 in adipocytes increased the expression of Ucp1 and Heme Oxygenase 1 (Hmox1), the principal Nrf2 target gene, suggesting the functional interaction of Nrf2 with β3-AR signaling. The activation of Nrf2 by tert-butylhydroquinone and reactive oxygen species (ROS) production by glucose oxidase induced both Ucp1 and Hmox1 expression. The increased expression of Ucp1 and Hmox1 was significantly reduced in the presence of a Nrf2 chemical inhibitor or in Nrf2-deleted (knockout) adipocytes. Furthermore, Nrf2 directly activated the Ucp1 promoter, and this required DNA regions located at -3.7 and -2.0 kb of the transcription start site. The CL316,243-induced Ucp1 expression in adipocytes and oxygen consumption in obese mice were partly compromised in the absence of Nrf2 expression. These data provide additional insight into the role of Nrf2 in β3-AR-mediated Ucp1 expression and energy expenditure, further highlighting the utility of Nrf2-mediated thermogenic stimulation as a therapeutic approach to diet-induced obesity.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.2
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• pp.139-146
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• 2021

Mitochondrial NADP+-dependent isocitrate dehydrogenase 2 (IDH2) produces NADPH, which is known to inhibit mitochondrial oxidative stress. Ureteral obstruction induces kidney inflammation and fibrosis via oxidative stress. Here, we investigated the role and underlying mechanism of IDH2 in unilateral ureteral obstruction (UUO)-induced kidney inflammation using IDH2 gene deleted mice (IDH2-/-). Eight- to 10-week-old female IDH2-/- mice and wild type (IDH2+/+) littermates were subjected to UUO and kidneys were harvested 5 days after UUO. IDH2 was not detected in the kidneys of IDH2-/- mice, while UUO decreased IDH2 in IDH2+/+ mice. UUO increased the expressions of markers of oxidative stress in both IDH2+/+ and IDH2-/- mice, and these changes were greater in IDH2-/- mice compared to IDH2+/+ mice. Bone marrow-derived macrophages of IDH2-/- mice showed a more migrating phenotype with greater ruffle formation and Rac1 distribution than that of IDH2+/+ mice. Correspondently, UUO-induced infiltration of monocytes/macrophages was greater in IDH2-/- mice compared to IDH2+/+ mice. Taken together, these data demonstrate that IDH2 plays a protective role against UUO-induced inflammation through inhibition of oxidative stress and macrophage infiltration.