• Title/Summary/Keyword: Dehydrogenase

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Effect of Consequent Application of Pig Manure Compost on Soil Chemical Properties and Dehydrogenase Activity in Volcanic Ash Soil (돈분퇴비 연용이 감자재배 화산회토양의 화학성과 탈수소 효소활성에 미치는 영향)

  • Joa, Jae-Ho;Moon, Doo-Gyung;Won, Hang-Yeon;Koh, Sang-Wook;Hyun, Hae-Nam;Lee, Chong-Eon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.3
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    • pp.283-288
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    • 2010
  • This study were carried out to evaluate effect of consequent application of pig manure compost (PMC) on soil chemical properties, dehydrogenase activity, and yield of potato in volcanic ash soil. The more application rate of PMC increased, the more increased soil pH, total-nitrogen, available phosphate, exchangeable cations (K, Ca, and Mg), heavymetal (Zn and Cu)contents. When application rate of PMC and crop cultivation times increased gradually, soil dehydrogenase activity was significantly increased. After third cultivation period, dehydrogenase activity showed PMC 2 ton (3.5), PMC 4 ton (6.3), PMC 6 ton (8.0 ug TPF $g^{-1}\;24h^{-1}$), respectively. The activity was twofold higher than first cultivation period. During the third cultivation period, dehydrogenase activity increased linearly comparison to Cu and Zn contents and that was correlated with Cu ($R^2$=0.907) and Zn ($R^2$=0.859) content, respectively. As the application rate of PMC increased, the yield of potato increased, but NPK+PMC 2 ton treatment was more higher than other treatments.

Cell Biological Studies on Brain Formation at the Early Stage of Chick Embryogenesis (초기계배의 뇌 형성에 관한 세포 생물학적 연구)

  • 최임순;주충노;최춘근;김재원;주상옥
    • The Korean Journal of Zoology
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    • v.29 no.3
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    • pp.215-233
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    • 1986
  • The effect of tryptophan on brain formation at the early stage of chick embryo has been investigated morphologically using electron microscope. The electron micrographs of cerebral cortex cells of $5\\sim10$ day old chick embryo, which received 1.0mg of tryptophan showed that the irregularity, evagination and disruption of nuclear membrane and nuclear chromatin condensation, nucleolar chromatin margination and segragation. Hypertrophy of stalks, vesicles, and vacuoles can be seen and dilation and vesiculation of rough endoplamic reticulum and polysome disaggregation occured. Protein and RNA levels and the activity of several enzymes such as lactate dehydrogenase, succinate dehydrogenase, malate dehydrogenase and glucose 6-phosphate dehydrogenase of tryptophan administered group were significantly lower than those of control group suggesting that the tryptophan administration depressed protein biosynthesis resulting in the decrease of enzyme activity. It was found that serotonin content of egg yolk which has been incubated for 10 days were as much as three times that of control egg yolk. It is not clear whether the increase of serotonin content might inhibit intracellular yolk granule degradation which might result in malformation of chick embryo, but it is likely that tryptophan administration might depress protein biosynthesis, consequently, the enzyme biosynthesis would be impaired. This might give rise to improper development of chick embryo.

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Lactate Dehydrogenase Isozyme of Hypoxia Tropical Catfish(Pangasius Polyuranodon, Hypostomus Plecostomus) (저산소 환경에 서식하는 열대성 catfish (Pangasius Polyuranodon, Hypostomus Ple-Costomus)의 젖산탈수소효소 동위효소)

  • 조성규;염정주
    • Journal of Life Science
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    • v.14 no.4
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    • pp.702-707
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    • 2004
  • In native-polyacrylamide gel electrophoresis of Pangasius polyuranodon, the lactate dehydrogenase (EC 1.1.1.27, LDH) $A_4$, $A_3$B, $A_2$$B_2$,$AB_3$ and $B_4$ isozymes were expressed in various tissues. The LDH $A_4$ and liver-specific $C_4$ isozymes were expressed in the tissues of Hypostomus Plecostomus. The bands of LDH in skeletal muscle, heart and eye tissues were not detected while one band was detected in kidney and liver, and four bands were detected in brain. The detected one band in liver was identified as alcohol dehydrogenase and an anodal band of skeletal muscle was identified as nothing dehydrogenase. The LDH in skeletal muscle, heart and eye might function as pyruvate reductase. The degree of inhibitions of LDH in skeletal muscle and heart of P. polyuranodon by 10 mM pyruvate were measured 57.6% and 73.8%, respectively. However, those of LDH in tissues of H. plecostomus were measured 52.7-61.8% so tissue specificity did not appear. Therefore, H. ple-costomus might be more acclimated to hypoxic environment by anaerobic metabolism of LDH iso-zymes than P. polyuranodon.

Soil Dehydrogenase Activity and Microbial Biomass C in Croplands of JeJu Province (제주지역 농경지 이용유형별 토양 탈수소효소활성과 미생물체량)

  • Joa, Jae-Ho;Moon, Kyung-Hwan;Choi, Kyung-San;Kim, Seong-Cheol;Koh, Sang-Wook
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.2
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    • pp.122-128
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    • 2013
  • This study was carried out to evaluate the soil dehydrogenase activity and microbial biomass C with soil type and land use in cropland of JeJu region. Soil chemical properties, dehydrogenase activity, and microbial biomass C were analyzed after sampling from upland (50 sites), orchard (50 sites), paddy (30 sites), horticultural facility (30 sites) in March. Average pH values was at 6.3 in upland soil, however soil chemical properties showed a large spatial variations in both orchard and horticultural facility soil. The Zn and Cu contents increased by the continuous application of pig manure compost in some citrus orchard soil. Soil dehydrogenase activity and microbial biomass C were higher in non-volcanic ash than in volcanic ash soil regardless of land use type. Soil dehydrogenase activity was two to four times higher in upland than in the others. It was at 38.7 ug TPF $24^{h-1}g^{-1}$ in non-volcanic ash of upland soil. Microbial biomass C content was very high in horticultural facility soil and it showed at 216.8 $mg\;kg^{-1}$ in non-volcanic ash. Soil dehydrogenase activity showed a positive correlation with organic matter ($r^2$=0.59), Zn ($r^2$=0.65), and Cu ($r^2$=0.66) in non-volcanic ash horticultural facility soil. There was a negative correlation ($r^2$=0.57) between soil organic matter and dehydrogenase activity in volcanic ash upland soil.

미생물의 Gucose-6-phosphate dehydrogenase에 관한연구 호소 생산 균주 Leuconostoc mesenteroides의 분리 및 생산실험

  • 이경은;정태화;민태익;한문희
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1976.10a
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    • pp.189.4-190
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    • 1976
  • glucose-6-phosphate dehydrogenase를 생산하는 Leuconostoc속 균주를 김치로부터 약 500주 분리하여 그 중 활성이 가장 강한 한주를 분리하여 동정한 결과 Leuconostoc mesenteroides였다. 이 균주로부터 몇가지 효소생산 조건을 실험하여 다음과 같은 결과를 얻었다.(결과)

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