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http://dx.doi.org/10.5352/JLS.2004.14.4.702

Lactate Dehydrogenase Isozyme of Hypoxia Tropical Catfish(Pangasius Polyuranodon, Hypostomus Plecostomus)  

조성규 (청주대학교 생명유전통계학부 생명과학전공)
염정주 (청주대학교 생명유전통계학부 생명과학전공)
Publication Information
Journal of Life Science / v.14, no.4, 2004 , pp. 702-707 More about this Journal
Abstract
In native-polyacrylamide gel electrophoresis of Pangasius polyuranodon, the lactate dehydrogenase (EC 1.1.1.27, LDH) $A_4$, $A_3$B, $A_2$$B_2$,$AB_3$ and $B_4$ isozymes were expressed in various tissues. The LDH $A_4$ and liver-specific $C_4$ isozymes were expressed in the tissues of Hypostomus Plecostomus. The bands of LDH in skeletal muscle, heart and eye tissues were not detected while one band was detected in kidney and liver, and four bands were detected in brain. The detected one band in liver was identified as alcohol dehydrogenase and an anodal band of skeletal muscle was identified as nothing dehydrogenase. The LDH in skeletal muscle, heart and eye might function as pyruvate reductase. The degree of inhibitions of LDH in skeletal muscle and heart of P. polyuranodon by 10 mM pyruvate were measured 57.6% and 73.8%, respectively. However, those of LDH in tissues of H. plecostomus were measured 52.7-61.8% so tissue specificity did not appear. Therefore, H. ple-costomus might be more acclimated to hypoxic environment by anaerobic metabolism of LDH iso-zymes than P. polyuranodon.
Keywords
lactate dehydrogenase; isozymes; Pangasius polyuranodon; Hypostomus Plecostomus;
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