• Title/Summary/Keyword: Dehydrogenase

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Effect of Dietary Protein Content on the Toluene Metabolism in Rats (식이성 단백질 함량이 Toluene 대사에 미치는 영향)

  • 윤종국;김경순;전태원
    • Toxicological Research
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    • v.12 no.2
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    • pp.237-241
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    • 1996
  • To study an effect of toluene administration on the toluene metabolism in rats liver previously fed a low (casein 7%, LP) or standard (casein 20%, SP) protein diet, toluene (50% in olive oil) was given at 0.2 ml per 100 g body weights once daily during 4 days to the male rats. The content of hepatic cytochrome P-450 was higher in rats fed SP than those fed LP. The hepatic benzylalcohol dehydrogenase activity was higher both in toluene-treated rats and its control group fed SP than those fed LP. The hepatic benzaldehyde dehydrogenase activity was somewhat higher in rats fed SP than those fed LP. In the case of toluene treatment, the increasing rate of hippuric acid contents to the control group were higher in rats SP than those fed LP. In conclusion, it is likely that the metabolic rate of toluene would be higher in rats fed SP than those fed LP.

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Kinetic Studies of Parent Compounds and Its Metabolite by Combined Treatment of Allyl Alcohol with Ethanol in vivo (Allyl Alcohol 및 Ethanol 혼합투여에 의한 혈중 농도 변화 및 독성과의 상관성)

  • 이주영;정승민;이무열;정진호
    • Toxicological Research
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    • v.14 no.4
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    • pp.557-562
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    • 1998
  • Allyl alcohol is metabolized in the liver through two steps, first to reactive acrolein by alcohol dehydrogenase (ADH), subsequently to acrylic acid by aldehyde dehydrogenase (ALDH). Since ethanol could compete the same enzymes to be metabolized in the liver, we have determined the plasma concentrations of allyl alcohol and ethanol followed by combined treatment. Pretreatment of rats with 2g/kg ethanol followed by ip administration of 40mg/kg allyl alcohol increased the lethality significantly. Determination of in vivo blood concentrations revealed that ethanol pretreatment caused the apparent decrease in allyl alcohol clearance, whereas acetaldehyde level in blood increased significantly by allyl alcohol treatment, as determined by head space GC analysis. Treatment of 4-methylpyrazole, an inhibitor of ADH, delayed allyl alcohol elimination significantly and reduced its lethality. Collectively, these findings suggested that reduction of allyl alcohol clearance in the presence oj ethanol was mediated through ADH competitive inhibition.

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Inhibitory Activities of Chinese Herbs that Clear Heat on $3{\alpha}-Hydroxysteroid$ dehydrogenase (한방청열제의 $3{\alpha}-Hydroxysteroid$ dehydrogenase에 대한 억제효과)

  • Ahn, Jung-Su;Choi, Seung-Youn;Kwon, Yong-Soo;Kim, Chang-Min
    • Korean Journal of Pharmacognosy
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    • v.29 no.1
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    • pp.8-12
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    • 1998
  • $3{\alpha}-Hydroxysteroid$ dehydrogenase $(3{\alpha}-HSD)$ is one of the main enzymes involved in the metabolism of the active androgen, dihydrotestosterone. The NAD(P)-linked $3{\alpha}-HSD$ of rat liver cytosol is powerfully inhibited by the non-steroidal anti-inflammatory drugs in rank-order of their therapeutic potency. This observation has now been developed into a rapid screen for predicting the potency of products that show anti-inflammatory effect. 52-Chinese Herbs that clear heat were screened by using this method.

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