• Applied Microscopy
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• 제28권3호
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• pp.299-306
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• 1998

T-butyl alcohol (TBA) freeze-drying method originally designed by Inoue and Osadake (1989) was adopted to dry specimens for scanning electron microscopy and the results were compared with those dried using critical point dryer (CPD). Small pieces $(1\times1\times3mm)$ of liver of Sprague-Dawley rats were cut and fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer after anesthesia, and processed for scanning electron microscopy by several modifications of TBA freeze-drying methods and by the standard preparation method using CPD. The bile canaliculi and sinusoidal endothelial surface were observed. Tissue dehydrated with TBA before TBA freeze-drying preserved the structures best comparable to those prepared with CPD. This result suggests that combination of dehydration with TBA and TBA freeze-drying is a superior method to the original TBA freeze-drying method dehydrated with ethanol.

• Clinical and Experimental Reproductive Medicine
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• 제29권4호
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• pp.245-250
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• 2002

Objective: To evaluate the efficacy of sclerotherapy for conservative treatment of ovarian pseudocyst. Methods: Patients with ovarian pseudocyst who had undergone sclerotherapy at Ajou University Hospital from February, 1997 to March, 2002 were included in this study. Sclerotherapy was performed as follows: Under intravenous analgesia, the cyst was irrigated with dehydrated alcohol after aspiration under transvaginal sonography. The aspirated contents and alcohol irrigated contents were sent for cytologic examination. Results: 71 patients with ovarian pseudocyst were performed sclerotherapy. All but 2 patients were followed up for 0.5 to 33 months with transvaginal sonography and pelvic examination. Mean age of the patients was 40 years old. Mean size of the pseudocyst was 8.0 cm, mean amount of aspiration fluid was 179.8 ml, and average follow-up duration was 6.0 months. All had abdominal or pelvic surgical history and 38 patients (58.9%) of them had undergone hysterectomy. 44 of 71 patients (62.0%) were performed sclerotherapy only one time. 27 patients (38.0%) recurred and were undergone more than one time. Complication was not occurred in all cases. Conclusions: We concluded that although pseudocyst after sclerotherapy has high recurrence rate, sclerotherapy was an safe and effective method for conservative treatment of ovarian pseudocyst.

• 한국식품조리과학회:학술대회논문집
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• 한국식품조리과학회 2005년도 추계학술대회 및 정기총회
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• pp.56-65
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• 2005

This study was purposed to investigate the antioxidative effects, the enzyme activity of the alcohol metabolizing and melanin production of Maesil(Prune mume). The antioxidant activity of Maesil(Prunu mume) was analyzed by measuring thiobarbituric acid reactive substances(TBARS value) and electron donating ability. And we investigated the changes of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH) activity by measuring the maximum absorbency at 340nm in vitro and human study. The inhibitory effects of Maesil were investigated in vitro and in B-16mouse melanoma cells on melanin biosynthesis that is closely related to hyperpigmentation. The antioxidant activities for TBA values were 29.65% in ascorbicacid, 45.35% in BHT, 15.99% in extract of dehydrated maesil flesh(EDMF) and 25.00% in extract of dehydrated maesil juice(EDMJ). The electron donating abilities by DPPH were 96.69% in ascorbic acid, 77.82% in BHT, 34.25% in EDMF, and 42.99%in EDMJ. Electron donating abilities by DPPH in the presence of 0.02% EDMF and EDMJ were 53.21% and 59.19% respectively. Facilitating rates of ADH activity were 137.92, 131.58, 152.96, 218.70, 111.76, and 144.27% in maesil juice, 5, 10, and 15% GMT, and 0.5 and 1.0% aspartic acid, respectively. ALDH activity increased in the order of Maesil juice > ALDH > GMT > aspartic acid, and facilitating rate of ALDH activity in Maesil juice was the highest at 976.44%. Maesil extracts inhibited tyrosinase activity that converts dopa to dopachrome in the biosynthesis process. B-16 cells treated by Maesil extracts showed that the viability was over 80%. Maesil and maesil products in vitro and B-16 cells inhibited melanin production significantly.

• Carbon letters
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• 제2권3_4호
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• pp.170-175
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• 2001

A petroleum-based isotropic pitch fiber spun by melt-blown method was oxidized in air flow at various conditions. The oxidized pitch fiber obtained was tested for its infusibility and its elemental composition during the process of stabilization. The structural changes were traced by using solvent solubility, FT-IR spectroscopy, and elemental analysis. The samples showed a gradual increase in weight with increasing the oxidization temperature. The weight gain of sample oxidized at $320^{\circ}C$ for 10 min was about 4.5%. The concentration of the pyridine and toluene soluble fraction decreased with an increase in stabilization temperatures. The oxygen uptaken in the stabilization process converted aliphatic side chains into the carbonyl groups. As stabilization proceeded, the more ether and carboxylic acid groups were formed through the oxidations of aldehyde and primary alcohol, and then the carboxylic acid was dehydrated to be aromatic anhydride.

• Applied Microscopy
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• 제1권1호
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• pp.27-34
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• 1969

The compound eyes of the adult Drosophila melanogaster were. fixed in 1.25 per cent glutaraldehyde and 1 per cent osmium tetroxide buffered with sodium cacodylate at pH 7.2. Double fixed specimens were dehydrated using the alcohol series and embedded in Epon 812. They were sectioned with porter blum and JUM 5B ultra-microtome and then stained with lead hydrooxide and uranyl acetate. All thin sections were examined with Hitachi HS-7 or HU-11 electron microscope. The rhabdomere of the compound eye is composed of numerous microvilli packed, arranged, and projected from inner edge of each retinal cell. Each microvillus consisted of a centrum, about $82{\AA}$ in diameter, surrounded by the substances, about $105{\AA}$ in width, which were bounded with double membrane about $44{\AA}$ in thickness. In each inner edge of the microvilli, there was a cylinder, about $175{\AA}$ in diameter, in parallel with retinal cells, which contained a cylindrical axis about $583{\AA}$ in diameter. The surface of the outer edges .of .the microvilli was bounded with reticullar substances about $500{\AA}$ in thickness.

• The Korean Journal of Pain
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• 제1권2호
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• pp.164-170
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• 1988

상북부 통증을 호소하는 암성통증 환자 18예, 만성 췌장염환자 4 예에서 통증제거 목적으로 60% dehydrated ethanol 30~50ml을 방척추법으로써 복강신경총의 지각전도를 차단한 후 얻은바 결론을 다음과 같이 요약한다. 1. 위암, 간암, 췌장암등으로 인한 상복부 통증에 대해서 86%의 우수한 진통효과가 있었다. 2. 만성췌장염 환자에서도 양호한 효과를 얻을 수 있었다. 3. 진통효과의 지속시간은 55%에서 약 4개월 내지 7개월간 지속되었다. 4. 시술후 일시적인 기립성 혈압하강이 16%에서 나타났다. 5. 시술후 합병증은 경미한 정도에서 안면홍조 혈압하강 요통등이 있었다. 6. 시술전 마약성 약물 사용이 습관화된 경우는 시술후 마약성 약물 투여금단 효과를 억제하기 어려웠다.

• 보존과학회지
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• 제31권2호
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• pp.95-104
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• 2015

주조철제유물은 발굴 후 보존환경이 맞지 않은 경우 부식이 급속하게 진행되며 특히 블록형 파손이 발생하고 최종에는 분말화 된다. 따라서 부식 요인, 즉 $Cl^-$ 이온을 제거하는 탈염처리가 중요하다. 그러나 주조철제유물은 탈염과정 중 형태가 파손되는 경우가 빈번히 발생하여 탈염 과정을 생략하거나 탈수 등의 안정화처리로 그치는 경우가 많다. 기존의 탈염 방법은 주로 물을 이용한 알칼리 수용액 방법이다. 그러나 물의 수산화이온은 부식을 촉진시키고 높은 표면 장력으로 내부 균열을 확대시켜 주조철제유물의 탈염 과정 중 파손 원인으로 판단된다. 따라서 본 연구에서는 표면 장력이 낮은 에틸알코올을 이용하여 탈염을 실시하고 그 탈염 효과를 알아보았다. 실험 결과 에틸알코올 탈염방법은 상온의 알칼리 수용액 방법과 유사하거나 더 높은 $Cl^-$ 이온 용출 효과를 보여주었다. 또한 에틸알코올 방법은 수용액 방법보다 유물의 파손이 적고 Fe의 용출이 거의 없어 탈염의 안전성 측면에서 우수함을 확인하였다. 본 실험은 현장적용 가능한 유기 용매 탈염 방법을 검토한 것으로 추가 연구를 통하여 주조철제유물의 안전하고 효율적인 안정화 방안을 마련하고자 한다.

• The Korean Journal of Pain
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• 제12권1호
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• pp.87-94
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• 1999

Backgroud: We have performed the CT-guided celiac plexus block (CPB) using anterior approach to evaluate the safety and efficacy of the procedure and to determine the role of CT. Methods: CPB were done in 10 patients (5 men and 5 women: mean age, 58.1 years) with intractable upper abdominal pain due to terminal malignancy of the stomach (n=3), pancreas (n=4), gallbladder (n=2), and liver (n=1). To permit an anterior approach, patients lay supine on the CT scan table during the procedure. One 21-guage Chiba needle was placed just anterior to the diaphragmatic crus between the celiac and superior mesenteric arteries and 10~12 ml of dehydrated alcohol was injected. Degree of pain relief following the procedure was assessed and pain was graded on a numeric rating scale (NRS) from 0 to 10. Results: The results suggest a direct relation between the degree of celiac invasion and the response to the CPB. With CT guidance, it is possible for us to direct the needle into more accurate region, allowing alcohol to be deposited in specific ganglion area. Conclusions: CT-guided CPB using an anterior approach was an easy and effective way of reducing intractable upper abdominal pain due to terminal malignancies. CT-guidance allowed precise needle placement and safe procedure. Careful classification of cases is important to predict the degree of pain relief using the grading system based on the degree of involvement of the celiac plexus.

• Applied Microscopy
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• 제1권1호
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• pp.11-17
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• 1969

Conidio spores of Aspergillus niger (strain No. NRRL 330) cultured on potato dextrose agar media were studied by electron microscopy, using the thin sectioning techniques. Conidio spores to be sectioned were fixed by triple methods with $K_2Cr_2O_7$, Glutaraldehyde and $OsO_4$. After dehydrated with alcohol, the specimens were embedded in metacrylate and epon resin media, and thinly sectioned by Porter-Blum MT-2. After sectioned these specimens were negative-stained with uranyl acetate and observed. by Hitachi HS-6 electron microscope. The results of this experiment were summarized as follows. 1. The structures of spore ,wall system seem to be formed 4 layers; exosporium, basal layer, spore coat and unit cell membrane. The protuberance of spore surface that was looked like hair appears to be protrusived from the basal layer. 2. The 3 layers of unit cell membrane was constituted outer layer membrane, inner layer membrane and inter-mediate light layer. 3. The structures of intra cytoplasmic membrane appear as spiral form which was consisted of 3 layers membrane system; outer membrane, inner membrane, and intermediate layer, which has pits. 4. The cement substance of spore coat and cortex may be changed quantitatively by physiological state in cell. 5. In some cases, we observed that the ribosome was transformed into poly ribosome group, and the storage materials and the protein crystals were changed variously. It. has been suggested that the morphological change of some cytoplasmic materials may be caused by some specialized function of the physiological stage.

• Applied Microscopy
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• 제14권2호
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• pp.81-93
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• 1984

Structural differences in various divisions of the rabbit colon were investigated using light and scanning electron microscopy. For light microscopic study, various Portions of the colon from seven rabbits (2.5 kg body weight) were fixed in 10% neutral buffered formalin, and paraffin sections were stained with hematoxylin-eosin. Tissues for scanning electron microscopy were fixed in 1% glutaraldehyde-1.5% paraformaldehyde and postfixed in 1% $OsO_4$, dehydrated to 100% alcohol, transfered to isoamilacetate and dried by the critical point method. Subsequently, specimens were coated with gold and viewed with a JSM-35C scanning electron microscope. The colon displays a morphological diversity along its proximo-distal axis. Five regions can be discerned based on the macroscopic and microscopic characteristics. 1) The first segment immediately distal to the cecocolical junction possessing three teniae is approximately 5 cm ($4{\sim}6cm$) in length, and displays irregular folds of the mucosa oriented transversely similar to those of the cecum. 2) The second segment possessing three teniae is about 7 cm ($5{\sim}8cm$) in length, and is characterized by the papilla-like protrusions on the mucosal surface. 3) The third segment, possessing a single tenia is about 16 cm ($12{\sim}20cm$) in length, and also displays the papilla-like protrusions similar to the aforegoing segment. 4) Fusus coli, approximately 4 cm ($3{\sim}5cm$) in length, is free of teniae and exhibits longitudinal folds on the mucosal surface. These four portions together constitute the proximal colon. 5) The distal colon reaches a length of about 58 cm ($53{\sim}55cm$) and shows a pattern of surface irregularities with minor ridges on the mucosal folds.