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Molecular Cloning and Phylogeny of the Human Endogenous Retrovirus HERV-W LTR Family in cDNA Library of Human Fetal Brain (인간 태아의 뇌로부터 만들어진 cDNA library에서 내생 레트로바이러스 HERV-W LTR의 클로닝 및 분자계통분류)

  • 이주민;허재원;신경미;이지원;이영춘;백인호;장경립;김희수
    • Journal of Life Science
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    • v.11 no.4
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    • pp.379-384
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    • 2001
  • Long terminal repeats(LTRs) of the human endogenous retrovirus(HERV) heve been found to be coexpresed with genes located nearby. It has been suggested that the LTR elements have contributed to the genetic variation of human genome connected to various diseases. Recently, HERV-W family was identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using cHNA library derived from human fetal brain, we performed PCR amplification and identified seven new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity(98∼99%) with HERV-W (AF072500). A phylogentic tree obtained by the neighbor-joining method revealed that seven new HERV-W LTR elements(FB-1, 2, 4, 8, 9, 10, 12) were closely related to the AX000960, AF072504, and AF072506 from Gen Bank database. Our data suggest that several copy numbers of the HERV-W LTR elements are expressed in human feta brain and may contribute to an understanding of biological function connected to neuropsychiatric diseases.

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Early Vegetational Succession of Burned Area in Mt. Ssalibagu (싸리바구山 山火跡地의 初期植生 邊移)

  • Kim, Jong Hong;Han Sung Jang
    • The Korean Journal of Ecology
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    • v.8 no.2
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    • pp.109-117
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    • 1985
  • This report is a part of the investigations of the secondary vegetation successions carried out and the analysis of soil properties in the burned areas of forest. The fires-crown fire and surface fire-were occurred at April, 1978 and February, 1984. The investigations were carrited out from August 10. 1983 to September 25, 1984. The burned areas studied are located in southern slope of Mt. Ssalibagu (above sea-level, 590m), So-myon, Sungju-gun, Chollanam-do, Korea. The results are as follows: The floristic compositions of the burned area at the 5th year after the fires were 85 families, 250 genera, 321 species and 53 varieties. Among them, 85 families, 127 genera, 129 species and 30 varieties were found in the currently burned area. In all the sampling sites (10*10cm), 31 and 57 species were found in the currently burned and in the 5th years after the fires, respectively. Miscanthus sinensis var. purpurascens, Quercus serrata and Festuca ovina were dominant species in the both areas. Biological spectra in both the burned areas showed $H-D_1-R_5-e$ type. Degree of the succession(DS) was 412-884 in the 5th years passed burned area and it was high level. Species diversity index(H) was 0.59~1.13 and evennes index(e) was 0.43~0.79, these indexes between both areas were different considerably. Indexes of similarity between both areas were different, too, but that between $B_1$ and $B_6$ was the highest (CCj=0.5). Probably this phenomenon is due to the great numbers of the pine trees appeared simulataneously. Content of the organic matter, N, P, K and Ca of soil in the burned area at the 5th years after the fires was lower than that of soil in the currently burned area.

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Effects of Eicosapentaenoic Acid during In Vitro Maturation of Porcine Oocytes: Hormone Synthesis and Embryonic Developmental Potential (에이코사펜타인산이 돼지난포란의 체외 성숙에 미치는 영향)

  • Kim, Kang-Sig;Park, Hum-Dai
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.222-231
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    • 2019
  • Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 μM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 μM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

A Study on the Construction method to improve the fuzzy controllers using language variable and coefficient selecting method (언어변수 및 계수선택방법을 이용한 퍼지제어기 설계에 관한 연구)

  • 박승용;변기녕;황종학;김흥수
    • Proceedings of the Korea Society for Industrial Systems Conference
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    • 2000.05a
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    • pp.125-134
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    • 2000
  • In this paper, we proposed a new circuit construction method that reduced the number of CMOS devices of singleton fuzzy controller(SFC) through the proposing a new membership function circuit(MFC) which uses the language variable selecting and the coefficient selecting circuit. According to the range of input values, we can choose the language variables beforehand which will be used in the inference. So we proposed the new MFC which generates the only necessary language variables. Also, we removed all rules of which adapting degree of their antecedents is zero through proposing the coefficient selecting circuit which beforehand selects the coefficients which will influence the inference result. Though this method, we simplified the structure of SFC and reduced the size of hardware. And to solve the problem in the current mode with respect to the restriction of the fan-out number, voltage-input and current-out membership function circuits are constituted of operational transconductance amplifiers. A membership function circuit which includes the language variable selecting circuit, a minimum operation circuit we implemented by current mode CMOS devices. As a result of applying proposed method, total numbers of blocks and devices wave decreased. If the number of variables and antecedents are getting larger, this method is more efficient.

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Effectiveness of Therapeutic Sports Massage in Delayed Onset Muscle Soreness (지발성근육통에 있어서 치료적 스포츠 마사지의 유효성)

  • Chang, Chung-Hoon;Jeong, Dong-Hyeog
    • The Journal of Korean Physical Therapy
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    • v.13 no.2
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    • pp.359-371
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    • 2001
  • The purpose of this study is to find out the effectiveness of the therapeutic sports massage(TSM) applied to the patients with delayed onset muscle soreness(DOMS) by measuring, assessing and analyzing the changes in intensity and unpleasantness of muscle pains before and after TSM. In the therapeutic sports massage program, such methods as effleurage, petrissage and deep transverse friction were selected as traditional massage treatments frequently used for muscles with pain and spasm. Effleurage and petrissage were applied for 20 minutes in total before and after deep transverse friction treatment. After TSM, the McGill pain questionnaire word list(MPQWL), verbal rating scale(VRS), visual analogue scale(VAS) were used to measure the degree of the pain on the patients. The major findings from this study are as follows; 1. The surveyed patients range from 15 to 63 in age, with highest numbers of 18(37.50%) registered in the twenties and next ones of 14(29.17%) in the thirties. Divided by sex, 27 are men and 21 are women totalling 48 with average age of 25.7. 2. There was significant decrease in the numerical values of VAS & VRS and MPQWL immediately after TSM(p<.05). There was also significant decrease in the numerical values of MPQWL, VRS and VAS after the 2nd, 3rd, 4th, 5th TSM(p<.05). 3. There was significant decrease in the intensity and unpleasantness of pains after TSM(p<.05). 4. From the analysis into chronological changes in the intensity and unpleasantness of pains before and after TSM with ANOVA, it became evident that the longer the period of treatment was, the higher the pains decreases drastically, while significant difference was shown in the intensity and unpleasantness of pains(p<.05). Summed up, it can be generally concluded that TSM is an effective treatment to rid the patients with DOMS of pains safely and promptly.

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Research on the Structure and Application of Fuzzy Environmental Impact Assessment Model

  • Tien, Shiaw-Wen;Hsneh, Chia-Hsiang;Chung, Yi-Chan;Tsai, Chih-Hung;Yu, Yih-Huei
    • International Journal of Quality Innovation
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    • v.5 no.2
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    • pp.45-62
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    • 2004
  • Any business activities may have impact on environment to a certain extent. Enterprises must find appropriate approaches to measure the impact on these environmental aspects, which can be used as the basis to direct enterprises' efforts to improve the environmental impact. The method used to evaluate significant factors in life cycle assessment standards is the one most commonly used by enterprises in general to measure environmental impact. By this method, the decisive factors of each environmental aspect are given scores according to the preset scoring standard of the organization. The scores are added up for each aspect and ranked to assess major environmental aspects. The drawback of this assessment method, that is, it ignores the degree to which each of these factors affects the environment, results in poor credibility. Therefore, this study attempts to solve some qualitative problems by applying to fuzzy theory, in particular, by identifying appropriate fuzzy numbers through fuzzy sets and membership function. Moreover, the study seeks to obtain a crisp value in the process of defuzzifization in order to make up for the shortfall of the original method in dealing with relative weight of decisive factors and thus increase its applicability and credibility. The department of light production of an electronics company is used as an example in this study to measure environmental aspects by employing both the traditional significant factor method and the fuzzy environmental impact assessment model proposed in this study. Based on verification and comparison of results, the model proposed in this study is more feasible as it reduces partiality in decision-making by taking the relative weights of decisive factors into consideration.

Cytotoxicity of resident and Iymphokine-activated mouse peritoneal macrophage against yrichomonas vaginalis (질트리코모나스(Trichomonas waginazis)에 대한 마우스 복강 대식세포의 세포독성)

  • Yu, Jae-Suk;An, Myeong-Hui;Min, Deuk-Yeong
    • Parasites, Hosts and Diseases
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    • v.28 no.2
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    • pp.85-90
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    • 1990
  • This study was aimed to observe the direct and Iymphokine-activated cell mediated cytotoxic effects against Trichomenas waginalis by mouse peritoneal macrophages. Cytotoxicity was measured as release of 3H-thymidine from prelabeled protozoa, and tested in U-bottom microtiter plates. A 0.1 ml suspension of labeled protozoa (2{\times}10^5/ml$) was placed in each well, followed by 0.1 ml of a suspension containing increasing numbers of peritoneal cells. After a 24 hr incubation at $37^{\circ}C$, 0.1ml of the supernatant was collected and counted in liquid scintillation counter. Mouse peritoneal macrophages had appreciable level of spontaneous cytotoxicity against T. maginalis at the effector to target cell ratios from 5 : 1 to 50 : 1, Treatment of macrophages with Iymphokine, produced by PHA-stimulated spleen cells, increased the cytotoxicity in comparison with resident macrophages against T. vaginalis. The degree of macrophage activation for the killing was not dependent upon the Iymphokine concentration. Peritoneal cells adherent to plastic displayed significant levels of cytotoxicity against T. vaginalis. This study indicates that mouse peritoneal macrophages are spontaneously cytotoxic for T. waginalis and Iymphokine increases the cytotoxicity by activating macrophages to kill T. vaginalis.

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Accuracy and Consistency of Three-Dimensional Motion Analysis System (3차원 동작분석 시스템의 정밀도와 측정 일관성)

  • Park, Young-Hoon;Youm, Chang-Hong;Seo, Kook-Woong
    • Korean Journal of Applied Biomechanics
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    • v.15 no.2
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    • pp.83-92
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    • 2005
  • Computer-assisted motional analysis is a popular method in biomechanical studies. Validation of the specific system and its measurement are fundamental to its application in the areas. Because the accuracy and consistency of a particular system provide the researchers with critical information to assist in making judgements regarding the degree to which inferences can be drawn from measurement data. The purpose of this study was to assess the accuracy and consistency of the Kwon3D motion analysis system. Validation parameters were five lengths from eight landmarkers in combination with the DLT reconstruction error values, digitizing monitor resolutions, and numbers of control points. With the best setting, Kwon3D's estimations of 260cm, 200cm, 140cm, 100cm, and 20cm were $260.33{\pm}.688cm$, $199.98{\pm}.625cm$, $139.89{\pm}.537cm$, $99.75{\pm}.466cm$, $20.08{\pm}.114$, respectively. There was no significant DLT error value difference between two monitor resolutions, but 0.27cm significant difference in 260cm estimation. There were significant differences in 260cm and 200cm estimations between with 33-control-point DLT error and with 17-control-point DLT error, but no in 140cm, 100cm, and 20cm estimations. Test-retest results showed that Kwon3D measurements were highly consistent with reliability coefficients alpha of .9263 and above.

A Study on the Customer Shopping Behavior based on the Store Image (점포이미지가 소비자 쇼핑행동에 미치는 영향)

  • Kang, Sung-Joo
    • The Journal of Information Technology
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    • v.7 no.4
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    • pp.89-101
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    • 2004
  • This study, Based on the study theories of up to the present was to a consumer's shopping motive, the process of choice and the theory of a consumer's store choice by his or her preference. It investigates the components of store image referring many thesis from documents of domestic and foreign countries. The major findings of from study an as follows: First, as general characteristics of samples, the component ratio of women is much higher than that of men. As for marital status, married consumers are large in numbers, and for educational back ground, high educated young couples go shopping frequently at department stores. As for ages, consumers under the 30s take great part in, for the occupation, many are housewives and for the average income of a month, under one million won income earners are major consumers. Second, about shopping motive, married ones do it for refreshing themselves, but for singles, they do shopping for the pleasure of bargain sales. The most important reason which influences on the shopping motive is 'pleasure of bargain sales' regardless of all ages. Third, according to the analysis of shopping motive and the characteristics of 15 store images, there are noteworthy differences statistically in shopping motive, attraction and reliance on advertisement, an atmosphere of a store and the degree of crowdedness.

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Analysis of Extension Pattern for Network of Movie Stars from Korea Movies 100 (한국영화 100선에 등장하는 영화배우 네트워크 확장 패턴 분석)

  • Ryu, Jea-Woon;Kim, Hak-Yong
    • The Journal of the Korea Contents Association
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    • v.10 no.7
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    • pp.420-428
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    • 2010
  • The advancement of the Science for complex systems enables the analysis of many social networks. We constructed and analyzed a Korean movie star network as one of social networks, based on the 100 Korean movie selection for a main data source. Until now, the research trend has been the structural analysis of network, focused on link numbers, such as degree, betweenness and clustering coefficient. But it is time that the research is not limited by the structural analysis of networks only. Rather, the research goal should be aimed to an information analysis, performed by identifying and analyzing central modules that are regarded as the core of complex networks, using k-core analysis method. In this research, we constructed a network of movie stars who have appeared in 100 Korean movie selection, provided by Korean movie database, also we analyzed its core modules with and without weights, and the trend of seasonal expansion of the network. We expect our findings can be used as the basic data applicable to a model for understanding of the expansion and evolution of networks.