• Mycobiology
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• 제40권1호
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• pp.20-26
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• 2012

The complexes of tailor made ligands with life essential metal ions may be an emerging area to answer the problems of multi drug resistance. The coordination complexes of VO(II), Co(II), Ni(II) and Cu(II) with the Schiff bases derived from isatin with 3-chloro-4-floroaniline and 2-pyridinecarboxaldehyde with 4-aminoantipyrine have been synthesized by conventional as well as microwave methods. These compounds have been characterized by elemental analysis, molar conductance, electronic spectra, FT-IR, FAB mass and magnetic susceptibility measurements. FAB mass data show degradation of complexes. Both the ligands behave as bidentate and tridentate coordinating through O and N donor. The complexes exhibit coordination number 4, 5 or 6. The Schiff base and metal complexes show a good activity against the bacteria; $Staphylococcus$ $aureus$, $Escherichia$ $coli$ and $Streptococcus$ $fecalis$ and fungi $Aspergillus$ $niger$, $Trichoderma$ $polysporum$, $Candida$ $albicans$ and $Aspergillus$ $flavus$. The antimicrobial results also indicate that the metal complexes are better antimicrobial agents as compared to the Schiff bases. The minimum inhibitory concentrations of the metal complexes were found in the range 10-40 ${\mu}g/mL$.

• 한국정보전자통신기술학회논문지
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• 제9권4호
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• pp.414-418
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• 2016

본 논문은 저면적 gradient magnitude 연산을 위한 하드웨어 구조를 제안한다. 하드웨어 복잡도를 줄이기 위해 정사영 벡터의 특징 및 하드웨어 자원 공유기법을 이용했다. 제안된 하드웨어 구조는 gradient magnitude 연산 알고리즘의 변형 없이 구현되었기 때문에 gradient magnitude 데이터 품질의 열화 없이 구현될 수 있다. 제안된 저면적 gradient magnitude 연산 하드웨어는 Altera Quartus II v15.0 환경에서 Altera Cyclone VI (EP4CE115F29C7N) FPGA를 이용하여 구현되었다. 구현 결과, 기존 하드웨어 구조를 이용하여 구현한 연산기와의 비교에서 15%의 logic elements 및 38%의 embedded multiplier 절감 효과가 있음을 확인했다.

• 한국재난정보학회 논문집
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• 제12권1호
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• pp.1-9
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• 2016

국부적인 두께감소 문제에 대하여 직관부위를 대상으로 한 건전성 평가는 잘 알려져 있으나 엘보우를 대상으로 한 건전성 평가는 최근에 원자력 분야에서는 많은 연구가 이루어지고 있으나 석유화학 플랜트에서 이용되는 건전성 평가 지침서 중 하나인 API579 코드에는 아직 없는 실정이다. 이에 본 연구에서는 엘보우를 대상으로 엘보우의 외부(extrados)와 내부(intrados)에 두께 감소가 있다고 가정한 후 유한요소해석법을 이용하여 두께감소된 엘부우의 건전성평가 해석을 수행하였다. 본 해석 결과는 석유화학 플랜트에서 이용되는 엘보우의 건전성 평가에 이용될 수 있음을 보여주었다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2001년도 추계학술대회
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• pp.16-25
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• 2001

A genomic library of biphenyl-degrading strain Comamonas sp. SMN4 was constructed by using the cosmid vector pWE15 and introduced into Escherichia coli. Of 1,000 recombinant clones tested, two clones that expressed 2,3-dihydroxybiphenyl 1,2-dioxygenase activity were found (named pNB 1 and pNB2). From pNB1 clone, subclone pNA210, demonstrated 2,3-dihydroxybiphenyl 1,2-dioxygenase activity, is isolated. 2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO, BphC) is an extradiol-type dioxygenase that involved in third step of biphenyl degradation pathway. The nucleotide sequence of the Comamonas sp. SMN4 gene bphC, which encodes 23DBDO, was cloned into a plasmid pQE30. The His-tagged 23DBDO produced by a recombinant Escherichia coli, SG 13009 (pREP4)(pNPC), and purified with a Ni-nitrilotriacetic acid resin affinity column using the His-bind Qiagen system. The His-tagged 23DBDO construction was active. SDS-PAGE analysis of the purified active 23DBDO gave a single band of 32 kDa; this is in agreement with the size of the bphC coding region. The 23DBDO exhibited maximum activity at pH 9.0. The CD data for the pHs, showed that this enzyme had a typical a-helical folding structures at neutral pHs ranged from pH 4.5 to pH 9.0. This structure maintained up to pH 10.5. However, this high stable folding strucure was converted to unfolded structure in acidic region (pH 2.5) or in high pH (pH 12.0). The result of CD spectra observed with pH effects on 23DBDO activity, suggested that charge transition by pH change have affected change of conformational structure for 23DBDO catalytic reaction. The $K_m$ for 2,3-dihydroxybiphenyl, 3-metylcatechol, 4-methylcatechol and catechol was 11.7 $\mu$M, 24 $\mu$M, 50 mM and 625 $\mu$M.

• 한국농공학회논문집
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• 제47권7호
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• pp.67-75
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• 2005

It has been well-known that the Nonpoint Source (NPS) pollutions are the primary contributors to water quality degradation in the receiving water bodies as well as the Point Source (PS) pollutions. To develop an effective management practice for water quality improvement, pollutant loads must be first estimated. In many studies, the Numeric Integration (NI) method has been used because of its ease of application, irrespective of the total number of samples collected for each storm event. Thus, there have been needs for more accurate pollutant load estimation with a limited number of water quality samples. In this study, NI method and regression method using the USGS ESTIMATOR model were comparatively used to calculate the pollutant loads for the Wolgokri watershed, Gangwon Province. The $NO_{3}$-N, T-N, and T-P loads using NI method and ESTIMATOR model were 13.85 kg/ha, 45.92 kg/ha, and 1.887 kg/ha, and 11.93 kg/ha,43.20 kg/ha, and 1.650 kg/ha, respectively. The estimated loads using ESTIMATOR model were lower than those using NI method by $86\%$, $94\%$, and $87\%$. These discrepancies in the estimated loads using a different load estimation method could be explained in that the total number of samples were not sufficient enough for NI method. Thus, ESTIMATOR model is recommended for the frequently stream discharge and less frequently measured water quality data.

• Journal of Ecology and Environment
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• 제40권1호
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• pp.34-44
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• 2016

Background: Little is known about how chemical water quality is associated with ecological stream health in relation to landuse patterns in a watershed. We evaluated spatial characteristics of water quality characteristics and the ecological health of Dongjin-River basin, Korea in relation to regional landuse pattern. The ecological health was assessed by the multi-metric model of Index of Biological Integrity (IBI), and the water chemistry data were compared with values obtained from the health model. Results: Nutrient and organic matter pollution in Dongjin-River basin, Korea was influenced by land use pattern and the major point sources, so nutrients of TN and TP increased abruptly in Site 4 (Jeongeup Stream), which is directly influenced by wastewater treatment plants along with values of electric conductivity (EC), bacterial number, and sestonic chlorophyll-a. Similar results are shown in the downstream (S7) of Dongjin River. The degradation of chemical water quality in the downstream resulted in greater impairment of the ecological health, and these were also closely associated with the landuse pattern. Forest region had low nutrients (N, P), organic matter, and ionic content (as the EC), whereas urban and agricultural regions had opposite in the parameters. Linear regression analysis of the landuse (arable land; $A_L$) on chemicals indicated that values of $A_L$ had positive linear relations with TP ($R^2=0.643$, p < 0.01), TN ($R^2=0.502$, p < 0.05), BOD ($R^2=0.739$, p < 0.01), and suspended solids (SS; ($R^2=0.866$, p < 0.01), and a negative relation with TDN:TDP ratios ($R^2=0.719$, p < 0.01). Conclusions: Chemical factors were closely associated with land use pattern in the watershed, and these factors influenced the ecological health, based on the multimetric fish IBI model. Overall, the impairments of water chemistry and the ecological health in Dongjin-River basin were mainly attributes to point-sources and land-use patterns.

• 동의생리병리학회지
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• 제18권2호
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• pp.553-560
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• 2004

In the present study, we investigated the anti-proliferative effects of aqueous extract of Wikyung-tang(WKT) on the growth of human lung carcinoma cell line A549. WKT treatment declined the cell viability and proliferation of A549 cells in a concentration-dependent manner. The anti-proliferative effects by WKT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. WKT treatment induced an inhibition and/or degradation of apoptotic target proteins such poly(ADP-ribose) polymerase (PARP) and phospholipase C-γ1 (PLC-γ1). WKT treatment did not affect the levels of other Bcl-2 family gene products, such as Bcl-2, Bax and Bad. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were induced by WKT treatment in A549 cells. Additionally, WKT treatment induced the down-regulation of telomerase reverse transcriptase mRNA (hTERT) expression of A549 cells, however, the levels of other telomere-regulatory gene products were not affected. Taken together, these findings suggest that WKT-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products and WKT may have therapeutic potential in human lung cancer.

• 한국항공우주학회지
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• 제36권10호
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• pp.939-946
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• 2008

압축성 유동장 해석을 위해 개발된 전산유체역학 프로그램의 응용범위를 확장하여 비압축성 유동장을 해석하고자 할 경우 해의 수렴성에 문제가 발생할 수 있다. 이러한 수렴성 저하 문제는 지배방정식의 수학적 특성에 기인하며 예조건화 방법을 이용하면 지배방정식의 큰 수정 없이 해결할 수 있다. 본 논문은 예조건화 기법을 적용한 압축성 유동해석 프로그램을 개발하여 수렴성의 문제없이 저속의 비압축성 유동장 해석의 적용에 관한 내용을 다루고 있다. 개발된 프로그램의 정확성과 수렴특성을 확인하기 위해 정상상태의 비점성 및 층류 그리고 난류 문제에 대하여 해석하였으며 이를 실험치와 비압축성 계산치와 비교하였다

• 한국신뢰성학회지:신뢰성응용연구
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• 제18권3호
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• pp.193-200
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• 2018

Purpose: In this study, the life of the motor is investigated by performing the accelerated life test with the brush wear of the industrial cleaner motor as the main failure mode. Methods: The accelerating stress factor of the accelerated life test is a voltage, which can increase the number of revolutions of the motor to accelerate the brush wear due to the friction between the brush and the commutator. Also, the accelerating stress level was determined after determining the maximum allowable level of the voltage through the preliminary test. Results: The motor failure time at each accelerating stress level was predicted by regression analysis with brush wear length as performance degradation data. The main failure mode, which is brush wear, of the motor was reproduced by this test. The shape parameter of the Weibull distribution was confirmed to be the same statistically at all accelerating stress levels by the likelihood ratio test. Conclusion: The life of the motor was investigated by performing the accelerated life test with the brush wear of the industrial cleaner motor as the main failure mode. Through the accelerating test method of the cleaner motor, various life expectancy and life expectancy of the acceleration factor are predicted.

• Clinical and Experimental Reproductive Medicine
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• 제40권1호
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• pp.33-37
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• 2013

Autophagy is a degradation process that acts in response to environmental stressors. Recently, autophagy has been detected in normal term, preeclamptic and intrauterine growth-restricted placentas. The object of this work was to investigate the presence of autophagy in first trimester voluntary interruption of pregnancy placental villi by the expression of autophagy-related proteins, light chain 3 (LC3), and Beclin-1. In first trimester placental villi laser scanning confocal microscopy (LSCM) analysis revealed LC3 and Beclin-1 immunoreactivity prevalently located in villous cytotrophoblasts. Using LSCM, LC3, and Beclin-1 were localized to the cytoplasm of the trophoblast layer in human full-term placentas. Beclin-1 expression and LC3 activation were confirmed by western blotting. These data emphasize that autophagy activation is different among cytotrophoblasts and syncytiotrophoblasts depending on the gestational age and thus we speculate that autophagy might play a prosurvival role throughout human pregnancy.