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Induction of Cdk Inhibitor p21 and Inhibition of hTERT Expression by the Aqueous Extract of Wikyung-tang in Human Lung Carcinoma Cells  

Choi Hae-Yun (Pohang Oriental Hospital of Daegu Haany University, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
Park Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
Choi Yung Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
Park Dong Il (Department of Internal Medicine, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.2, 2004 , pp. 553-560 More about this Journal
Abstract
In the present study, we investigated the anti-proliferative effects of aqueous extract of Wikyung-tang(WKT) on the growth of human lung carcinoma cell line A549. WKT treatment declined the cell viability and proliferation of A549 cells in a concentration-dependent manner. The anti-proliferative effects by WKT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. WKT treatment induced an inhibition and/or degradation of apoptotic target proteins such poly(ADP-ribose) polymerase (PARP) and phospholipase C-γ1 (PLC-γ1). WKT treatment did not affect the levels of other Bcl-2 family gene products, such as Bcl-2, Bax and Bad. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were induced by WKT treatment in A549 cells. Additionally, WKT treatment induced the down-regulation of telomerase reverse transcriptase mRNA (hTERT) expression of A549 cells, however, the levels of other telomere-regulatory gene products were not affected. Taken together, these findings suggest that WKT-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products and WKT may have therapeutic potential in human lung cancer.
Keywords
Wikyung-tang(葦莖湯); PARP; PLC-γ1; p53; p21; hTERT;
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  • Reference
1 Premature p34cdc2 activation required for apoptosis. /
[ Shi. L.;Nishioka, W.K.;Th'ng, J.;Bradbury, E.M.;Litchfield, D.W.;Greenberg, A.H. ] / Science
2 Multiple pathways to apoptosis /
[ Evans, V.G. ] / Cell Biol. Int.   DOI   ScienceOn
3 Apoptosis. The role of the endonuclease. /
[ Arends, M.J.;Morris, R.G.;Wyllie, A.H. ] / Am. J. Pathol
4 Induction of bax by genotoxic stress in human cells correlates with normal p53 status and apoptosis /
[ Zhan, Q.;Fan, S.;Bae, I.;Guillouf, C.;Liebermann, D.A.;OConnor, P.M.;Fornace, A.J., Jr ] / Oncogene
5 Mammalian G1 cyclins. /
[ Sherr, C.J. ] / Cell
6 Cyclin A is required for the onset of DNA replication in mammalian fibroblasts /
[ Girard, F.;Strausfeld, U.;Fernandez, A.;Lamb, N.J. ] / Cell   DOI   ScienceOn
7 A link between cyclin A expression and adhesion- dependent cell cycle progression /
[ Guadagno, T.M.;Ohtsubo, M.;Roberts, J.M.;Assoian, R.K. ] / Science   DOI
8 Role for cyclin A in the dependence of mitosis on completion of DNA replication /
[ Walker, D.H.;Maller, J.L. ] / Nature   DOI   ScienceOn
9 Cdk inhibitors: on the threshold of checkpoints and development /
[ Elledge, S.J.;Harper, J.W. ] / Curr. Opin. Cell Biol.   DOI   ScienceOn
10 The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases /
[ Harper, J.W.;Adami, G.R.;Wei, N.;Keyomarsi, K.;Elledge, S.J. ] / Cell   DOI   ScienceOn
11 Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit /
[ Gu, Y.;Turck, C.W.;Morgan, D.O. ] / Nature   DOI   ScienceOn
12 p21 is a universal inhibitor of cyclin kinases /
[ Xiong, Y.;Hannon, G.;Zhang, H.;Casso, D.;Kobayashi, R.;Beach, D. ] / Nature   DOI   ScienceOn
13 Complex regulatory mechanisms of telomerase activity in normal and cancer cells: How can we apply them for cancer therapy /
[ Kyo, S.;Inoue, M. ] / Oncogene   DOI   ScienceOn
14 Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT) /
[ Poole, J. C.;Andrews, L. G. and Tollefsbol, T. O. ] / Gene   DOI
15 /
[ 통계청 ] / 2001년 사망원인통계결과
16 /
[] / 東醫肺系內科學
17 /
[ 王燾 ] / 外臺秘要
18 /
[ 孫思邈 ] / 備急千金要方
19 葦莖湯·加味葦莖湯의 A549에 대한 세포독성과 S-180에 대한 항암효과 /
[ 박경식;박정휘;김동희;김성훈 ] / 동의병리학회지
20 /
[ 김상찬;김선희;노승현;박선동;변승희;서부일;서영배;이상인;이강희;주영승;최호영 ] / 方劑學
21 Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells /
[ Bae, S.S.;Perry, D.K.;Oh, Y.S.;Choi, J.H.;Galadari, S.H.;Ghayur, T.;Ryu, S.H.;Hannun, Y.A.;Suh, P.G. ] / FASEB J.   DOI
22 Apoptosis-induced cleavage of -catenin by caspase-3 results in proteolytic fragments with reduced transactivation potential /
[ Steinhusen, U.;Badock, V.;Bauer, A.;Behrens, J.;Wittman- Liebold, B.;Dorken, B.;Bommert, K ] / J. Biol. Chem.   DOI   ScienceOn
23 Studies of inositol phospholipid-specific phospholipase C /
[ Rhee, S.G.;Suh, P.G.;Ryu, S.H. and Lee, S.Y. ] / Science   DOI
24 Overexpression of phospholipase C-1 in rat 3Y1 fibroblast cells leads to malignant transformation /
[ Chang, J.S.;Noh, D.Y.;Park, I.A.;Kim, M.J.;Song, H.;Ryu, S.H.;Suh, P.G. ] / Cancer Res
25 Activation of phosphatidylinositol 3-kinase is important for erythropoietin-induced erythropoiesis from CD34(+) hematopoietic progenitor cells /
[ Myklebust, J.H.;Blomhoff, H.K.;Rusten, L.S.;Stokke, T.;Smeland, E.B. ] / Exp. Hematol.   DOI   ScienceOn
26 p53-dependent apoptosis modulates the cytotoxicity of anticancer agents /
[ Lowe, S.W.;Ruley, H.E.;Jacks, T. and Housman, D.E. ] / Cell   DOI   ScienceOn
27 Bcl-2 prolongs cell survival after Bax- induced release of cytochrome c /
[ Rosse, T.;Olivier, R.;Monney, L.;Rager, M.;Conus, S.;Fellay, I.;Jansen, B.;Borner, C. ] / Nature   DOI   ScienceOn
28 Bcl-2 functions in an antioxidant pathway to prevent apoptosis /
[ Hockenbery, D.M.;Oltvai, Z.N.;Yin, X.M.;Milliman, C.L.; Korsmeyer, S.J. ] / Cell   DOI   ScienceOn
29 Principles of CDK regulation /
[ Morgan, D.O. ] / Nature   DOI   ScienceOn
30 DNA damage-induced cell cycle checkpoints involve both p53- dependent and -independent pathways: role of telomere repeat binding factor 2 /
[ Narayan, S.;Jaiswal, A.S.;Multani, A.S.;Pathak, S. ] / Br. J. Cancer   DOI   ScienceOn
31 Telomeres, telomerase, and myc. An update /
[ Cerni, C. ] / Mutat. Res.   DOI   ScienceOn
32 Apoptosis and the cell cycle /
[ Chiarugi, V.;Magnelli, L.;Basi, G. ] / Cell. Mol. Biol. Res.
33 WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis /
[ El-Deiry, W.S.;Harper, J.W.;O'Connor, P.M.;Velculescu, V.E.;Canman, C.E.;Jackman, J.;Pietenpol, J.A.;Burrell, M.;Hill, D.E.;Wang, Y.;Wiman, K.G.;Mercer, W.E.;Kastan, M.B.;Kohn, K.W.;Elledge, S.J.;Kinzler, K.W.;Vogelstain, B. ] / Cancer Res
34 Tumor suppressor p53 is a direct transcriptional activator of the human bax gene /
[ Miyashita, T.;Reed, J.C. ] / Cell   DOI   ScienceOn
35 Significance of cell cycle and checkpoint control. /
[ Choi, Y. H. and Choi, H. J. ] / Kor. J. Life Sci.
36 The Pezcoller lecture: cancer cell cycles revisited. /
[ Sherr, C.J. ] / Cancer Res.
37 The retinoblastoma protein and cell cycle control. /
[ Weinberg, R.A. ] / Cell   DOI   ScienceOn
38 D-type cyclin-dependent kinase activity in mammalian cells /
[ Matsushime, H.;Quelle, D.E.;Shurtleff, S.A.;Shibuya, M.;Sherr, C.J.;Kato, J.Y ] / Mol. Cell. Biol.   DOI
39 Identification of G1 kinase activity for cdk6, a novel cyclin D partner. /
[ Meyerson, M.;Harlow, E. ] / Mol. Cell. Biol.   DOI
40 Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle /
[ Koff, A.;Giordano, A.;Desai, D.;Yamashita, K.;Harper, J.W.;Elledge, S.;Nishimoto, T.;Morgan, D.O.;Franza, B.R.;Roberts, J.M. ] / Science   DOI
41 Cyclin-dependent regulation of G1 in mammalian fibroblasts /
[ Ohtsubo, M.;Roberts, J.M. ] / Science   DOI
42 Evaluation of in vitro chemosensitivity using human lung cancer cell lines /
[ Kratzke, R. A. and Kramer, B. S. ] / J. Cell. Biochem. Suppl.
43 Ayurvedic herbal drugs with possible cytostatic activity. /
[ Smit, H.F.;Woerdenbag, H.J.;Singh, R.H.;Meulenbeld, G.J.;Labadie, R.P.;Zwaving, J.H. ] / J. Ethnopharmacol.   DOI   ScienceOn
44 Yama/CPP32 , a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase /
[ Tewari, M.;Quan, L.T.;O'Rourke, K.;Desnoyers, S.;Zeng, Z.;Beidler, D.R.;Poirier, G.G.;Salvesen, G.S.;Dixit, V.M. ] / Cell   DOI   ScienceOn
45 Specific proteolytic cleavage of poly (ADP-ribose) polymerase: an early marker of chemotherapy- induced apoptosis /
[ Kaufmann, S.H.;Desnoyers, S.;Ottaviano, Y.;Davidson, N.E.;Poirier, G.G. ] / Cancer Res.
46 Cleavage of poly ADP-ribose polymerase by a proteinase with properties like ICE /
[ Lazebnik, Y.A.;Kaufmann, S.H.;Desnoyers, S.;Poirier, G.G.;Earnshaw, W.C. ] / Nature
47 Cell adhesion molecules in the development and progression of malignant melanoma /
[ Johnson, J.P. ] / Cancer Metastasis. Rev.   DOI   ScienceOn
48 E-cadherin- catenin cell-cell adhesion complex and human cancer. /
[ Wijnhoven, B.P.;Dinjens, W.N.;Pignatelli, M. ] / Br. J. Surg.   DOI   ScienceOn
49 Induction of apoptosis by ursolic acid through activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells /
[ Choi, Y.H.;Bae, J.H.;Yoo, M.A.;Chung, H.Y.;Kim, N.D.;Kim, K.W. ] / Int. J. Oncol.
50 The role of the E-cadherin/catenin complex in gastrointestinal cancer /
[ Debruyne, P.;Vermeulen, S.;Mareel, M. ] / Acta. Gastroenterol. Belg.
51 Apoptosis-associated cleavage of -catenin in human colon cancer and rat hepatoma cells /
[ Fukuda, K ] / Int. J. Biochem. Cell. Biol.   DOI   ScienceOn
52 ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the post-translational activation of p53 protein involving poly(ADP-ribose) polymerase /
[ Vaziri, H.;West, M.D.;Allsopp, R.C.;Davison, T.S.;Wu, Y.S.;Arrowsmith, C.H.;Poirier, G.G.;Benchimol, S. ] / EMBO J.   DOI   ScienceOn
53 The proteolysis of mitotic cyclins in mammalian cells persists from the end of mitosis until the onset of S phase /
[ Brandeis, M.;Hunt, T. ] / EMBO J.