• Journal of Marine Life Science
• /
• v.1 no.1
• /
• pp.18-24
• /
• 2016

Since April 2012, doctor fish in the breeding tank and in the quarantine tank in Hanwha Aquaplanet Yeosu Aquarium have been dying, accompanied by diffuse bleeding around the mouth, in the chin, and at the bottom of the abdomen. In this study, the cause of death would be examined through the bacteriological study of doctor fish and the rearing water quality in the aquarium. The water quality and the bacterial counts of the rearing water in the exhibit tank and in the quarantine tank were analyzed once a week, starting from August to November 2014. Water quality was measured based on the following data: temperature was in the range of 24.5~26.8℃, pH at 6.77~7.94, DO at 6.15~8.61 ppm, ammonia at 0~0.93 ppm, nitrite at 0.009~0.075 ppm, and nitrate at 1.1~40.9 ppm. Studies revealed that the differences in these water quality factors were not related to the death of doctor fish. Bacterial counts in the rearing waters of Garra rufa slightly increased to 10³~10⁴ CFU/ml, just before the death of the doctor fish. Twelve strains of bacteria were isolated from the dead fish and rearing waters. The isolates were identified as Aeromonas veronii, Citrobacter freundii, Pseudorhodoferax aquiterrae, Shewanella putrefaciens, and Vibrio anguillarum on the basis of 16S rRNA gene sequences. The most dominant species was C. freundii, which showed medium sensitivity to florfenicol and norfloxacin, and was resistant to amoxacillin, doxycycline, oxytetracycline, tetracycline, and trimethoprim. Ten isolates were confirmed to be pathogenic to the doctor fish. Doctor fish infected with C. freundii and S. putrefaciens showed high mortality in the experimental groups. These results indicate that the variation in bacterial numbers in the rearing water was related to the death of doctor fish. C. freundii and S. putrefaciens were directly implicated in causing the death of doctor fish in the aquarium.

• Parasites, Hosts and Diseases
• /
• v.21 no.1
• /
• pp.11-18
• /
• 1983

As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nodus. The results obtained were summarized as follows: 1. The cercariae which attempted to contact with Cyprinus carpio nodus in the water were observed under the stereomicroscope. After contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the numbers ranged to 80% at 13 minutes after the encounter. But very few cercariae could actually invade into the epidermis of the fish. 2. When Cyprinus carpio nudes were exposed to a number of cercariae in the beaker, only a few cercariae could invade through the epidermis. Most of the invaded cercariae were killed before forming the cyst. Also rare encysted cercariae were found to be dead within 48 hours. 3. Cyprinus carpio nudes were reared with Parafossarulus manchouricus shedding numerous cercariae in the indoor aquarium. When observed after 12 days, to determine the susceptibility, no metacercaria was found infected. 4. Cyprinus carpio nudum were reared with Parafossarulus manchouricus shedding numerous cercariae in Kimhae lake for 2 months in summer of 1982, in order to check the natural infection. No metacercaria of Clonorchis sinensis was found from the fish. 5. In the cases of control fish, Pseudoraibora larva, numerous cercariae were inspected on the course of invasion through epidermis of the fish under the stereomicroscope. And many metacercariae were also found from the Ssh whenever they were kept in beaker, indoor aquarium, or the lake for natural infection. The results suggested that Cyprinus carpio kudus might not be proper intermediate host of Clonorchis sinensis.

• Journal of Institute of Control, Robotics and Systems
• /
• v.19 no.8
• /
• pp.682-687
• /
• 2013

Navigation is a crucial capability for all types of manned or unmanned vehicles. However, vehicle navigation in underwater environments still remains a challenging problem since GPS signals for position fixes are not available in the water. Terrain-referenced underwater navigation is an alternative navigation technique that utilizes geometric information of the subsea terrain to correct drift errors due to dead-reckoning or inertial navigation. Terrain-referenced navigation requires the description of an undulating terrain surface as a mathematical function or table, which often leads to a highly nonlinear estimation problem. Recently, PFs (Particle Filters), which do not require any restrictive assumptions about the system dynamics and uncertainty distributions, have been widely used for nonlinear filtering applications. However, PF has considerable computational requirements which used to limit its applicability to problems of relatively low state dimensions. This study proposes the use of a Rao-Blackwellized particle filter that is computationally more efficient than the standard PF for terrain-referenced underwater navigation involving a moderate number of states, and its performance is compared with that of the extended Kalman filter algorithm. The validity and feasibility of the proposed algorithm is demonstrated through numerical simulations.

• Journal of the Korean GEO-environmental Society
• /
• v.14 no.12
• /
• pp.13-22
• /
• 2013

A substructure of bridges is very important structural element for safety and supporting not only vertical loads as dead load and live load but lateral loads as break load, wind load, seismic load, hydrostatic pressure and dynamic water pressure, lateral earth pressure, impulsive load, temperature change and load effect of temperature change, creep and shrinkage. Most of domestic bridges are reinforced concrete piers and have an effect on economy of bridge. Recently, understanding importance of substructure, we are getting more interested in new substructure system.

• Korean Journal of Veterinary Research
• /
• v.47 no.3
• /
• pp.299-302
• /
• 2007

Neoheterobothrium hirame (N. hirame) is a large blood-feed gill-worm infecting the highly prized olive flounder. The present study was reported the occurrence and elimination of Neoheterobothrium infection from young cultured oliver flounder in the western coast of Korea. Flounder (weight 18-25 g, length 10-15 cm) were obtained from a cultured farm at Taean of Korea at May 2006. A lot of flounder died with serious anemia. We isolated N. hirame from the buccal cavity wall of dead flounder. Adult parasites were 5-15 mm in total length, with 4 pairs of clamps on the pedunculated haptor and isthmus region embedded in host tissue, while immature and sub-adults were identified the attachment to the gill filaments, rakers and arches with the clamps. All fish with infection were treated with 8% NaCl-supplemented seawater for 1 and 10 min at $22^{\circ}C$, respectively. The significant reduction in mortality (6.7%) was observed among fish treated with 8% salt water for 10 minutes, when compared with that of non-treatment group (100%).

• The Journal of Korean Medicine
• /
• v.20 no.2
• /
• pp.54-62
• /
• 1999

This experiment was carried out to study the safe utilization and standardization Whangryunhaedok-tang extract solution for herbopuncture on stroke patients. The test material was manufactured by water-alcohol method. The components of Whangryunhaedok-tang extract solution for herbopuncture were analyzed by HPLC. Mice were used for acute toxicity tests. The results were as follows. 1. In the berberine contents(%) of Whangryunhaedok-tang extract, berberine was 0.25%. 2. There were no abnormal findings in acute toxicity test treated with Whangryunhaedok-tang extract solution for herbopuncture and LD50 was not reached because no experimental animals were found dead throughout the study.

• The Journal of Internal Korean Medicine
• /
• v.25 no.2
• /
• pp.214-223
• /
• 2004

The study examines the anti-cancer effects of the hot water extract of Mylabris phalerata(MP) using SNU-C5 cell lines. Microscopic analysis showed that 12 hours after MP treatment, the number of dead cells increased prominently. Significant cell death was observed 12, 24, and 48 hours after MP treatment through trypan blue exclusion testing. This suggests that MP is time-dependently cytotoxic. Mitotracker Red CMXRos staining and flowcytometry revealed that MP decreased mitochondrial membrane potentials. The absence of peaks on PI staining showed that DNA damage occurred in MP treated cells. Taken together, measurements suggest that MP has a strong anti-cancer effect on SNU-5 cell lines, and that this is likely to be due to the destruction of mitochondria and DNA damage.

• Structural Engineering and Mechanics
• /
• v.50 no.4
• /
• pp.419-439
• /
• 2014

In this study, the structural performance of a seven span masonry arch bridge was evaluated. Investigations were performed on Aspendos (Belkis) Masonry Arch Bridge which was located on road of Aspendos Acropolis City in Antalya, Turkey. The old bridge was constructed in the early of fourth century AD, but it was exposed to the earthquakes in this region and the overloading by the river water. The old bridge was severely damaged and collapsed by probably an earthquake many years ago and a new bridge was then reconstructed on the remains of this old bridge by Seljuk in the 13th century. The bridge has also been affected from overflowing especially in the spring of each year, so some protective measures should be taken for this monumental bridge. Therefore, the structural performance under these loading has to be known. For this purpose, an initial finite element model was developed for the bridge and it was calibrated according to ambient vibration test results. After that, it was analyzed for different load cases such as dead, live, earthquake and overflow. Three load combinations were taken into account by deriving from these load cases. The displacements and the stresses for these combination cases were attained and compared with each other. The structural performance of Aspendos Masonry Arch Bridge was determined by considering the demand-capacity ratio for the tensile stress of the mortar used in Aspendos Masonry Arch Bridge. After these investigations, some concluding remarks and offers were presented at the end of this study.

• Analytical Science and Technology
• /
• v.13 no.3
• /
• pp.269-276
• /
• 2000

Chromatographic retention mechanism of seven benzoic acids in RPLC were investigated. Inorganic salt is pertinent for the measurement of dead time in benzoic acid. Logarthims of capacity factor (log k') and Hildebrand solubility parameter (${\delta}$) showed proportional relationship which imply the hydrophobic effect of the retention mechanism in RPLC. Enthalpies of solute transfer showed inverse proportion to temperature and organic modifier ratio of the mobile phase. It was found that the S value showed positive slope in plot of log k' vs. volume fraction of water in mobile phase. Free energy change increases with increasing organic volume fraction. The hydrophobicity index, ${\varphi}_0$(organic volume fraction) is inversely proportional to column temperature.

• Proceedings of KOSOMES biannual meeting
• /
• 2004.05b
• /
• pp.53-56
• /
• 2004

During the period of every summer to early autumn seasons, ships have been wrecked or grounded from effect of a typhoon in the water areas around Korean Peninsular. Typhoon Maemi. which brushed South Korea in September 12-13, 2003, left at least 110 dead or missing, knocking down building. smashing ships and triggering floods that forced about 25,000 to flee from their homes. In this work. records .of marine accidents caused by a typhoon are investigated for the period from 1962-2002. The distribution is also compared with the trajectories of typhoons, passed during the 1990-2003. In addition to that, attack frequency of typhoon and number of marine accidents is the highest in August