• 대한수의학회지
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• 제58권4호
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• pp.171-175
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• 2018

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

• 대한본초학회지
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• 제23권2호
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• pp.51-58
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• 2008

Objectives : Trimellitic anhydride (TMA), a sensitizer that induces occupational asthma and atopic dermatitis, is widely used industrially to make epoxy and alkyd resins, plasticizers, high temperature polymer, and surfactants. The aim of this study was to investigative the effects of aqueous extracts of Lonicera japonica Flower(LJFAE) on TMA-induced contact hypersensitivity (CHS) in Balb/c mice. Methods : The dried flowers of L. japonica were extracted with distilled water at $100^{\circ}C$ for 7 h. The extract was freeze-dried following filteration through 0.45 ${\mu}m$ filter. Mice were orally administrated with or without LJFE of a different doses(25-100 mg/kg) for 28 days. In the challenge period, mice were externally applied at difference doses of LJFAE one time per day 30 min before TMA treatment. We examined the effects of LJFAE on the the serum levels of IgE and prostagladin E2 (PGE2), the Thl/Th2 cytokine production of spleen cells, ear swelling responses, and the leukocyte infiltration induced by TMA. Results : The orally and externally administration of LJFAE dose-dependently reduced the serum levels of IgE and PGE2 production as well as ear swelling responses and leukocyte infiltration in TMA-induced Balb/c mice. Furthermore, the levels of Thl (TNF-${\alpha}$, IFN-${\gammer}$, IL-2)/Th2 (IL-4, IL-5, IL-13) cytokine production from spleen cells stimulated with anti-CD3 and CD28 mAbs was markedly suppressed by the orally and externally treatment with LJFAE in a concentration dependent manner. Conclusions : These results suggest that LJFAE suppresses the inflammatory mediators and regulates the Thl/Th2 cytokines. Therefore, these properties may contribute to the strong anti-CHS response effect of LJFAE.

• 원예과학기술지
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• 제29권5호
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• pp.494-499
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• 2011

Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing $130g{\cdot}L^{-1}$ sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing $130g{\cdot}L^{-1}$ sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at $4^{\circ}C$ without media. Among the various medium concentrations used ($0.25{\times}$, $0.5{\times}$, $1.0{\times}$, $2.0{\times}$, and $4.0{\times}$ NLN liquid medium), $0.5{\times}$ NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$, $0.5{\times}$, and $1.0{\times}$ NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$ NLN microelements, followed by incubation at $25^{\circ}C$ for 30 days.

• 원예과학기술지
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• 제29권5호
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• pp.500-502
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• 2011

1999년 'Bristol Fairy' 유래의 결실되는 아조변이체 Gyp99 계통을 선발하였고, 그 계통의 실생 중 계통선발을 거듭한 결과 특성이 우수한 Gyp06-29 계통을 선발하여 2년간 특성 검정을 통해 'Dream Pink'를 육성하였다. 'Dream Pink' 품종은 중간형의 초형을 가지고, 소화는 꽃잎수가 많고 암술이 2개인 분홍 겹꽃이며, 꽃잎은 도란형, 꽃받침 모양은 종형인 식물체 고유특성을 갖는다. 또한 화경이 강하고 흰가루병에 매우 강하고 충해에도 강한 편이다. 여름절화 작형에서 'Dream Pink' 개화는 대조품종인 'My Pink'보다 5일 늦고 절간장과 화경장이 길었으며, 마디와 측지 분화가 많고 분지각은 작았다. 소화와 꽃받침 크기가 크고 꽃잎수가 많았다.

• Current Research on Agriculture and Life Sciences
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• 제21권
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• pp.17-21
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• 2003

글라디올러스의 생육에 있어 엽초의 역할을 알아보기 위해 제거시기와 제거수를 달리하여 엽초제거 한 후 생육반응을 조사한 결과, 생육초기의 광조건에 관계없이 엽초를 모두 제거함으로써 화경장과 화수장이 짧아졌고 개화수도 적어졌으며 특히 제거시기가 빠를수록 생육이 현저하게 저조해졌다. 그러나 엽초제거에 따른 개화소요일수는 차이를 보이지 않았다. 엽초를 엽위별로 제거한 처리구에서 엽초 1, 2 및 3매를 제거한 것은 생육 차이를 보이지 않았으나 글라디올러스의 초장 생장에 비례적으로 자라난 4번째 엽초를 포함하여 제거한 엽초 4매 제거구에서 타 처리구에 비해 화경장이 25cm이상 작아졌으며 화수장도 짧아지고 개화수도 적어졌다. 또한 줄기기부도 비정상적으로 굵어졌다.

• 생물환경조절학회지
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• 제15권2호
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• pp.149-155
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• 2006

풋고추(청양)의 육묘 온도를 주간온도는 같게 하고 야간온도를 달리하여 유묘의 화아분화와 생장 및 포장에서의 생장을 조사하였다. 육묘기간 중의 야간 온도가 높을수록 고추 묘의 엽수, 지상부 생체중 및 건물중이 증가하였고, 화아분화 및 첫 개화일은 단축되었다. 정식 후 초장, 경경, 분지장, 절간장은 저온구($28/11^{\circ}C$)에서 증가한 반면 고온구($28/21^{\circ}C$)에서 억제되었다. 측지수는 고온구에서 현저히 감소되었으나 온도 처리에 따라 주경에서 발달하는 1차분지의 수는 일정한 경향을 보이지 않았다. 고추 모종의 생장은 저온구에서 억제되었다가 하우스에 정식한 후부터 점차로 회복된 반면, 고온구는 이와 반대의 경향을 보였다.

• 한국육종학회지
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• 제42권6호
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• pp.606-610
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• 2010

'드림문'은 경기도농업기술원에서 2008년도에 육성한 절화용 스프레이 계통의 국화 신품종이다. 이 품종은 2005년도에 황색 스프레이 국화 'Patra (yellow)'품종을 모본으로 하고 분홍색 'Sei-Rosa'품종을 부본으로 인공교배하여 화색과 화형이 우수한 12계통을 예비 선발하였다. 2006-2008년 까지 3차에 거쳐 특성검정과 품평회로 4번째 개체인 'GCS06-27-4'를 최종선발하여 '드림문(Dream Moon)'으로 명명하였다. '드림문' 자연개화기는 10월 하순경으로 전형적인 추국 형태의 품종이며, 고온기인 여름철과 관수를 많을 주었을 경우 화색이 아주 연한 분홍색이 될 가능성이 있어 철저한 관리가 요구된다. 분홍색(PG76D) 스프레이 형태로 3중의 꽃잎이 있는 홑꽃형이며 꽃잎수는 24.6매이고, 화경은 5.6 cm, 엽색은 녹색(Green Group 137B)으로 절화장은 86.6 cm로 중간종이다. 저온동계 검정에 의한 흰녹병 발생은 나타나지 않았으며, 절화 수명은 20.7일로 양호한 편이다.

• 한국연초학회지
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• 제14권2호
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• pp.104-115
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• 1992

The present study was conducted to compare the relative efficiency of four different breeding methods in tobacco varietal development. A single Fl hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L Bright Yellow 4(BY4) and NC95, was selfed. F2population above cross was screened for resistance against bacterial wilt caused by Pseudommonas solanacearum E.F.Smith under the naturally infested field conditions, and the 30 lines were developed from F2 individual plant by anther culture (ADH), maternal method utilizing Nicotiana afpicana (MDH), single- seed descent(SSD) and Bulk breeding method, respectively. All characters except content of total alkaloids of ADH and MDH which wore bred by haploid methods reduced more than that of lines bred by conventional methods(SSD & Bulk) : however, the yields were 8% lower than other lines. The total alkaloid content of ADH was higher than that of MDH, and yield was reduced about 4n even though the number of leaf was identical with the MDH. All other characters of ADH were also reduced significantly. In the lines bred by conventional methods, population developed by SSD showed significantly wider leaf width, shorter plant height, later days to flower, and lower in percent reducing sugar than those by Bulk. The populations derived from haploid method showed greater phenotypic variance and wider range of variation than conventionally developed ones. The results obtained indicate that selection will provide a significantly greater genetic gain for leaf number and leaf length in the ADU and MDH populations, and for plant height and days to flower in the SSD and Bulk populations.

• 한국토양비료학회지
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• 제48권6호
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• pp.677-682
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• 2015

Although it is not a landscape crop according to MIFAFF(ministry of agriculture, food and rural affairs), yellow mustard is considered a superb landscape crop because of its growth and flower characteristics. We sowed yellow mustard as a landscape and green manure crop in upland soil (four types of soil texture) in spring. And we studied its flowering characteristics, dry weight, and nitrogen yield. The growths of yellow mustard were possible in every soil ranging from sandy soil to clay loam. Its height was 54.1 ~ 76.1cm and the number of node per hill was 11.3 ~ 17.0. Its flowers had a light yellow. It took about 44 ~ 50 days to flower, and flowered for 22~25 days. The dry weight of yellow mustard was $2.27{\sim}3.60tonha^{-1}$ with highest in sandy loam and loam. Among the nutrients of yellow mustard, nitrogen(T-N) was $12.6{\sim}20.8gkg^{-1}$, and C/N ratio was 21.6~37.7. The nitrogen yield of yellow mustard was $35{\sim}62kgha^{-1}$. In conclusion, because of its flowering characteristics and dry weight, yellow mustard was considered appropriate for both green manure and landscape crop uses.

• 한국환경농학회지
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• 제39권4호
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• pp.360-367
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• 2020

BACKGROUND: The productivity and quality of blueberry (Vaccinium ashei Reade) greatly depend on the number of fruits in a plant. Especially, fruit set more than appropriate number negatively affects productivity and marketability due to the increased number of small fruits and delayed harvest time. This study was conducted to investigate proper timing and concentration for applying chemical blossom thinners such as ammonium thiosulfate (ATS) and UREA. METHODS AND RESULTS: ATS at 1.25% and 1.50%, and UREA at 6% and 8% were applied in four developmental stages, bud swell, pink bud, full bloom and petal fall. Fruit thinning rate was calculated based on the number of fruits harvested divided by that of flowers before applying blossom thinners. Ratios of leaf to flower and leaf to fruit were calculated based on the number of fully developed leaves in 25 days after full blossom divided by that of flowers or fruits, respectively. Chemical injury of leaves was investigated by calculating the number of leaves with chemical injury divided by the total number of leaves. Fruit thinning rates were 48% and 66% for UREA treatments at 6% and 8%, respectively, and 49% and 62% for ATS treatments at 1.25% and 1.50%, respectively, in the full bloom stage. In the petal fall stage, fruit thinning rates were 18% and 24% for UREA treatments at 6% and 8%, respectively, and 49% and 35% for ATS treatments at 1.25% and 1.50%, respectively. Leaf to fruit ratio (L/FR) increased by 109% and 188% compared to leaf to flower ratio in ATS treatments at 1.25% and 1.50%, respectively, and L/FR increased 93 and 196% in UREA treatments at 6% and 8%, respectively, in the full bloom stage. In the petal fall stage, leaf to fruit ratio increased by 60% to 100% in ATS treatments, but did not significantly differ from the control in UREA treatments. Fruit harvest was delayed in all treatments of all developmental stages except for 1.5% ATS and 6% UREA treatments at the petal fall stage, whose fruit harvest was two or three days faster than the control. CONCLUSION: The application of ATS and UREA for blossom thinning should be in the petal fall and full bloom stages for early and late harvest, respectively. Considering chemical injury, integrated harvesting and fruit size, however, it is appropriate to apply ATS at 1.5% in the petal fall stage to increase fruit productivity and quality in blueberry.