• 한국자원식물학회지
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• 제29권2호
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• pp.235-240
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• 2016

본 연구는 절화, 관상 및 의약소재로 이용가치가 높은 자생 숙은노루오줌의 종자 번식법을 개발하고자 수행되었다. 연구에 사용된 종자는 크기가 0.62×2.22 ㎜이며, 1,000립중은 40.5 ㎎으로 조사되었다. 종자의 기간별 수분흡수율을 조사한 결과, 2.57%였던 수분함량이 침지처리 24시간 만에 29.8%로 증가하였으며, 종피의 팽창이 관찰되었다. 숙은노루오줌의 종자는 저온요구성 광발아성으로 15℃, 명조건에서 최종발아율(40.8%)이 가장 높았으며, 발아상의 온도가 높아질수록 발아율은 낮아졌다. 암조건에서는 어느 온도조건에서도 전혀 발아되지 않았다. 종자에 생장조절제 및 무기염류를 처리한 결과, KNO₃는 농도에 관계없이 무처리구에 비해 우수한 발아촉진 효과를 나타내었다. 특히 KNO₃ 20 mM 처리구에서 최종발아율(77.8%)과 발아세(96.1%)가 향상되었으며, 평균발아소요일수(11.3일)와 T₅₀ (6.5일)도 효과적으로 단축시킬 수 있었다.

• 방사선산업학회지
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• 제6권2호
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• pp.189-197
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• 2012

This study was carried out to develop the improved useful mutants for yield or composition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds of stevia 'Suwon No. 11' were irradiated up to 400 Gy of gamma ray. Chemical mutagens were treated on the seeds of the 'Suwon No. 11' using 0.07% colchicine, 10 mM sodium azide, or 10 mM NMU for various durations. The germination rate, and shoot and root growth of seedling were estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and the plant height, the number of branches, and leaf length and width were examined at 3 months after mutagenesis treatments. In the case of gamma ray treatments, the germination rate and early-stage growth were decreased as the increase of radiation dose, and the 50% lethal dose was found to be 200 Gy. the plant height was decreased as the increase of radiation dose, while the number of branches per plant and leaf length were increased. Leaf shape was modified to the relatively longer one compared to the control, which was identified more apparently at the treatments of higher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survival were decreased as the increase of incubation time. The 50% lethal dose for germination rate were identified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mM sodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemical mutagens had no influence on shoot growth, while root growth was increased, especially as the incubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrs incubation time. The plant height was decreased as the increase of incubation time in the chemical mutagens treatments. Among the chemical mutagens, NMU was the most effective to induce the mutants with long-shaped or the least lobed leaves.

• 한국약용작물학회지
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• 제23권1호
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• pp.13-19
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• 2015

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, to test qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthes japonica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperatures and durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germination rates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than those in Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12 mm than those in Je-Cheon about 4.90 mm and seed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areas were higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing temperatures and durations, they were not clearly different in between $4^{\circ}C$ and $25^{\circ}C$ and they also were gradually decreased with getting longer storing durations. The germination rates treated by plant growth regulators were higher with $GA_3$ than those with Kinetin. The highest seed germination rate was appeared at 50 ppm of $GA_3$. Comparing its rates with different seed priming, they were relatively higher with $KNO_3$ than those with PEG6000. In protein expression patterns between before the germinating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination compared to those before the germination of seeds, especially 10 ~ 20 kDa. These results showing more and clear bands were more clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulators and seed primings, $GA_3$ was better expression than those with Kinetin and $KNO_3$ was better than those with PEG6000. More and clear bands were closely related to the germination rates of seeds and more detailed studies would be required.

• 한국작물학회지
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• 제41권1호
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• pp.53-61
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• 1996

필리핀은 화산지대에 위치해 있으며 44개 화산중 13개가 활화산으로 되어있는 상태이다. 전기발전을 위해 지하에 있는 고온의 온천수를 이용하고 있으며 그 후 시냇가에 방류한다. 이 온천수에는 독성이 강한 물질들이 많이 함유되어 있어 관개수로 이용하였을 경우 농작물에 지대한 악영향을 주고 있는 실정이다. 본 실험에서는 온천수를 이용하여 벼품종의 발아, 유묘생장유관속발달에 미치는 영향을 구명하여 작물 재배관리를 위한 기초자료를 얻고자 수해하였던 바 그 결과를 요약하면 다음과 같다. 1. 온천수내에는 황, 칼리, 나트륨, 및 붕소 함량이 관개수에 비해 현저하게 높았다. 2. 최종발아율, 발아소요일수 및 발아속도는 온천수의 처리에 의해 크게 감소하였으며, 필리핀재래종인 Binato품종은 IR58 및 운봉7호보다 높은 발아율을 보였다. 3. 초장, 근장, 엽수 및 총건물중은 온천수의 농도가 증가될수록 뚜렷하게 감소하였다. Binato와 IR58품종은 처리 후 15일, 25% 온천수 농도에서 운봉 7호보다 건물중이 다소 높았다. 4. 유묘기 엽신의 유관속발달은 무처리에 비하여 온천수는 50%까지 농도를 높일수록 유관속수 및 크기 등 발달을 저하시켰다. 5. 온천수 처리에 따른 엽의 손상도는 농도가 높아질수수록 크게 나타났으며, Bitano와 IR58호는 운봉 7호보다 온천수에 대한 손상도가 작아서 다소 강한 것으로 판단되었다.

• 한국약용작물학회지
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• 제26권4호
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• pp.291-295
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• 2018

Background: The use of Rehmannia glutinosa of the family Scrophulariaceae, in traditional medicine is well known. This study was conducted to elucidate the effect of air curing of the seed rhizome of R. glutinosa on its storability and yield. Methods and Results: The root of the R. glutinosa cultivar (Dagang) was harvested in at the end of November. The seed rhizomes were air cured for one to seven days. They were subsequently wrapped with newspaper and further stored in a plastic container at $1^{\circ}C$. It was observed that the weight loss and decay rates were significantly lower in the air cured seeds than in the untreated ones. Moreover, the decay rate of the control was approximately 50%, 120 days after storage. However. the decay rate of all the air cured treatment groups was less than 1%. Additionally, air curing led to an increase in the germination rate of the seeds and the root yield when compared with the untreated groups. Taken together, the ideal treatment period for air curing was found to be 3 days. Under these conditions, the germination rate and yield were 88.7% and 2,185 ㎏/10a, respectively. Conclusions: This study successfully demonstrated that the air curing of seed rhizomes can lead to a considerable increase in the storability and yield of R. glutinosa.

• 한국약용작물학회지
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• 제11권1호
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• pp.46-52
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• 2003

제주특산 식물인 섬오갈피나무(Acanthopanax koreanum NAKAI)의 다량증식을 위하여 번식방법 및 유묘 생육 상황을 조사한 결과는 다음과 같다. 1. 종자는 15 에서 60일간 후숙시킨 다음 Kinetin 50ppm에서 24시간 침지후 5 에서 60일간 저온처리 하여 휴면타파한 것이 발아율 64%였다. 2. 녹지삽에서 호르몬 농도처리시 발근율은 IBA 100ppm에서 61.7%, NAA 50ppm에서 56.7%, IAA 100ppm에서 60.0%였다. 3. 녹지삽목에서 상토재료별 루톤 분의 처리 시 발근율은 송이+원예상토에서 76.7%, 비화산회토양에서 66.7%로 효과적 이었다. 4. 3월 30일 삽목하여 8월 10일 정식묘에서 활착율은 75%, 차광망 설치구에서 96%로 좋았으며 지상부 생육상황은 55% 차광망 설치구에서 좋았다. 5. 9월 30일 삽목하여 다음해 5월 2일 정식묘에서 활착율은 55% 차광망 설치구에서 91%로 좋았으며, 생육 및 수량성은 55% 차광망 설치구에서 양호하였다. 6. 전정부위에 따른 줄기 재생력은 지상 30cm 높이에서 전정한 것이 생육이 좋았다.

• 한국약용작물학회지
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• 제4권3호
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• pp.231-235
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• 1996

작약(芍藥) 화분(花粉)의 형웅(形熊)와 화분발아능력(花粉發芽能力)에 관한 실험을 수행(遂行)한 결과를 요약(要約)하면 다음과 같다. 1. 작약(芍藥)의 화분형태(花紛形態)는 주(主)로 장타원형(長楕圓形)이었으며 작약화분(芍藥花粉)의 화분(花粉) 발아률(發芽率)은 71% 정도로 타계통보다 낮은 편이었다. 2. 꽃봉오리 상태에서 화분은 개화 10일전까지는 관찰되지 않았으나 개화전(開花前) $7{\sim}9$일 부터 관찰되었고 개화기(開花期)가 가까울수록 정상화정비률(正常花精比率)과 화분(花粉) 발아률(發芽率)이 높았다. 3. 작약화분(芍藥花粉)은 화분발아배지(花粉發芽培地)에서 치상(置床) 30분 후(後)부터 발아하기 시작하여 화분관(花粉管)이 신장(伸長)하였으며 약 2시간부터 3시간 사이에서 발아가 거의 완료(完了)되었다. 4. 화분(花紛)의 수명(壽命)은 저장기간(貯藏期間)이 경과(經過)할수록 단축(短縮)되었는데 상온(常溫)저장시 저장 8일에 51%. 15일에 25% 31일에는 전혀 발아되지 않았고 $5^{\circ}C$와 -$15^{\circ}C$에 15일 저장시는 각각50%, 55%가 발아되었으며 95일 저장에서도 각각 48%와 43%로 높은 발아율을 나타내어 $5^{\circ}C$냉장(冷藏)과 $-15^{\circ}C$냉동(冷凍)에서는 작약화분(芍藥花粉)의 장기저장(長期貯藏)이 가능(可能)하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.240-240
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• 2017

Quinoa (Chenopodium quinoa Willd.) is one of the ancient crops cultivated in the Andes region at an altitude of 3,500-4000m in Chile and Bolivia from 5000 BC. It contains a large amount of protein, minerals and vitamins in comparison with other crops. The cultivation area has been increasing worldwide because of its excellent resistance to various abiotic stress such as salinity, drought and low temperature. ${\gamma}$-Ray radiation of high dose is often used as a tool to induce mutations in plant breeding, but it has a deleterious effect on organisms. However, the radiation may have a positive stimulatory effect of 'hormesis' in the low dose range. This experiment was carried out to investigate the optimum dose range for creating the quinoa genetic resources and to investigate the hormesis effect at low dose on the quinoa. This experiment was performed for 120 days from November, 2016 to February, 2017 in the greenhouse of Gyeongsang National University. ${\gamma}$-Ray radiation was irradiated to seeds at 0 Gy, 50 Gy, 100 Gy, 200 Gy, 300 Gy, 400 Gy, 600 Gy, 800 Gy and 1000 Gy for 8 hours. (50 Gy) using the low level radiation facility ($Co^{60}$) of Cooperative Research Institute of Radiation Research Institute, KAERI. Fifty seeds were placed on each petri dish lined with wet filter paper and germination rate was measured at a time interval of 2 hours for 40 hrs. The length of the root length was measured one week after germination. Each treatment was carried out in 3 replicates. The growth of seedlings were investigated for 10 days after transplanting of 30 day-old seedlings. The plant height, NDVI, SPAD, Fv/Fm, and panicle weight were measured. The germination rate was highest at 50Gy and 0Gy and the rate of seeds treated with 400Gy or higher rate decreased to 25% of the seeds treated with 50Gy. The emergence rate of seedling in pot experiment was higher at the dose of 200 Gy, 300 Gy and 400 Gy than at 0 and 50Gy. However, the rate was lower at strong radiation higher than 600Gy at which $1^{st}$ leaf was not expanded fully and dead due to extreme overgrowth at 44 days after treatment (DAT). The highest value of panicle weight was observed at 50Gy (6.15g) and 100Gy (5.57g). On the other hand, the weight at high irradiated dose of 300Gy and 400Gy was decreased by about 55% compared to low dose (50 Gy). NDVI measurement also showed the highest value at 50 Gy as the growth progressed. SPAD was the highest at 400 Gy and showed positive correlation with irradiation dose except 0 Gy. Fv/Fm was high at 50 Gy up to 30 DAT and no difference between treatments was observed except for 400 Gy from 44 DAT. The plant height was the highest in 50Gy during the growing period and was higher in the order of 50Dy, 100Gy, 0Gy, 200Gy, 300Gy and 400Gy in 88 DAT. In this experiment, the optimal radiation dose for hormesis was 50Gy and 100Gy, and the optimal radiation dose for mutagenesis seems to be 400 Gy.

• 한국약용작물학회지
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• 제3권1호
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• pp.66-70
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• 1995

본(本) 연구(硏究)는 잔대(沙蔘) 종자(種子)의 특성(特性)을 조사(調査)하고 아울러 1993년에 채종(採種)한 종자(種子)를 재료(材料)로 하여 저온습윤(低溫濕潤), 고온(高溫) 및 Gibberellin 처리(處理)를 한 후 발아기간중(發芽期間中)의 명(明) 암조건(暗條件)과 발아온도(發芽溫度)을 구명(究明)하여 잔대종자(種字)의 발아율(發芽率) 향상(向上)을 위한 기초자료(基礎資料)를 활용코자 수행(遂行)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 종자(種子)의 종피(種皮)는 개화후(開花後) 30일에는 하얀색으로, 누르면 물렁거리는 상태(狀態)이었으나 40일경 부터는 종자(種子)가 갈색(褐色)을 띠면서 딱딱하였다. 2. 종자(種子)의 1,000입중(粒重)은 개화후(開花後) 40일에는 247mg이었으나 개화후(開花後) 50일에는 268mg으로 최대치에 달했으며 개화후(開花後) 60일에는 감소(減少)하는 경향(傾向)이었다. 3. 종자(種子) 발아율(發芽率)은 무처리(無處理) 40%에 비(比)하여 $0^{\circ}C$의 저온(低溫)에 7일간 습윤처리(濕潤處理)하였을때 88.3%, Gibberellin 100ppm 24시간 침윤처리(浸潤處理)하였을때 96.7%로 발아가 가장 양호(良好)하였다. 4. 잔대 종자(種子)는 암조건(暗條件)에서는 전혀 발아(發芽)되지 않고 명조건(明條件)에서 발아(發芽)가 잘 되었으나 Gibberellin 100ppm에 침지처리(浸漬處理)하면 암조건(暗條件)에서도 80% 발아(發芽)되었다. 5. 잔대 발아온도(發芽溫度)은 $25^{\circ}C$ 내외(內外)이었다.

• 현장농수산연구지
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• 제20권2호
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• pp.89-97
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• 2018

대목용 박은 개화 후 50~70일 정도 지난 종과를 수확하여 10~15일 간 후숙한 후 채종하고 있는데, 본 연구에서는 수정 후 종과의 수확시기와 후숙기간에 따른 발아율(발현율)을 비교하였다. 실험재료로는 2010년 한국농수산대학에서 정식하여 채종한 대목용 박 5품종을 이용하였다. 채종과의 수확은 수분 후 각각 24, 31, 50, 70일에 하였으며 후숙 기간은 수확 후 5일과 수확일과 후숙일을 더하여 85일이 되도록 각각 62, 55, 35, 15일 후에 채종하였다. 수분 후 24일 후에 채종한 종자는 전혀 발아하지 않았으며, 31일과 50일째 수확한 것이 경제적인 발현율을 보였다. 24일째에 수확한 종과도 62일간 후숙하면 78% 이상의 발현율을 보여 미숙과실도 효과적인 후숙을 통해 채종이 가능함을 보였고, 50일 이상 성숙한 종과의 경우 10-15일 정도의 후숙 처리를 하여도 발아율이 높은 종자를 채종할 수 있을 것으로 추찰되었다. 종자에 대한 배의 비율과 유묘의 하배축 길이와 굵기로 본 종자의 성숙도에 있어서도 후숙의 효과가 뚜렷하였으며, 24일째 수확하여 62일 후숙한 종자는 성숙한 종과에서 채종한 종자에 버금가는 성능을 보였다.