• 생물환경조절학회지
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• 제12권4호
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• pp.228-234
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• 2003

본 연구는 오이종자의 발아율 향상과 발아촉진을 위해 간편하고, 비용을 절감할 수 있는 hydropriming의 적정 환경조건을 구명하기 위해 수행되었다. 오이종자의 발아촉진에 최적 priming 처리제는 50 mL $NaNO_3$였다. 유근장 및 유근 건물중은 priming 처리제 종류에 따라 유의적인 차이는 없었으나, priming 및 hydropriming 종자는 무처리보다는 높았다. 그러나 50 mL $NaNO_3$와 hydropriming 처리간 발아력 증진 효과가 동일하여 오이종자에서는 물을 이용한 hydro-priming 처리가 처리비용을 절가할 수 있어 합리적인 종자처리였다. 오이종자의 hydropriming 적정 처리온도는 20$^{\circ}C$, 처리시간은 24시간이였다. Hydropriming시 종자 2g에 대한 용액량 10 mL 공급처리에서 발아율이 향상되었고 발아촉진에 좋아 적정 용액량이라고 판단되었다. 인위노화 종자는 발아율이 감소하였고 $T_{50}$ 및 MDG는 지연되었으며, 비정상적인 발아는 증가하였다. 활력이 저하된 조화종자를 ,hydropriming한 처리는 발아율이 향상되었고, 조기 발아하였다. 또한 비정상인 발아는 감소되었으며, 유근생장이 촉진되어 발아력이 부분적으로 회복되었다. 이러한 경향은 노화일수가 경과된 종자일수록, 부적당한 발아조건인 15$^{\circ}C$에서 현저하였다.

• 생물환경조절학회지
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• 제23권4호
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• pp.342-348
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• 2014

본 연구는 온도와 차광이 참나물 종자의 발아와 유묘의 생장에 미치는 영향을 조사하고자 실시하였다. 실험은 온도별($5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$) 발아 시험과 차광별(무차광, 35% 차광, 50% 차광, 75% 차광) 생육 시험의 두 종류로 실시되었으며, 발아실험 전 종자는 0일, 20일, 40일로 각각 저온습윤 처리를 하였다. 발아율은 20일 저온습윤 처리의 $5^{\circ}C$에서 48.3%로 가장 높았으며, 온도가 높아질수록 낮아지는 경향을 보였다. 차광에 따른 초장, 엽면적, 엽장, 엽폭 등은 모두 50% 차광에서 각각 7.3cm, $12.8cm^2$, 5.4cm, 2.3cm로 가장 컸다. 특히, 뿌리는 35%와 50% 차광에서 생장이 좋았다. 지상부의 생체중은 50% 차광과 75% 차광에서 가장 높았으며, 건물중은 35% 차광과 50% 차광에서 높았다. 본 실험결과를 종합적으로 살펴보면, 참나물 유묘는 50% 차광을 유지하여 생육하는 것이 이식용 유묘 생산에 효과적일 것으로 판단된다.

• Journal of Plant Biology
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• 제16권1_2호
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• pp.17-22
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• 1973

The experiments were conducted to examine the injuries of $SO_2$, $NH_3$, and $SO_2$ and NH3 mixed gas to the germination and the growth of plants. Six kinds of plants were used as the material. These plants seeds were treated with the gases for five days. Rate of the germination and the growth in height of varying plants were different according to the components of the gases. The critical concentration of the gases for both the germination and the growth were in 5ppm of $SO_2$, 50ppm of $NH_3$, and 50ppm $SO_2$ and 50ppm $NH_3$ mixed gases. When a low concentration of $SO_2$ was treated together with NH3, especially it was reduced to 60 percent of the damage in the germination and the growth. In the treatemnt with $SO_2$, the germination of the seeds which soaked in water for 24 hours reduced the injuries more 40 percent than those which for one hour. It was observed that the seeds with thick coasts or with originally intact coats were suffered but little damaged by the gases, and the external symptoms of an injury were shown, at first, in water pore, and then, in guard cell on the leaves.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.88-88
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• 2018

In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $KNO_3$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the $2^{rd}$day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the $3^{rd}$day after sowing), 2.6days MGT and $39%{\cdot}day^{-1}$ germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and $25%{\cdot}day^{-1}$ GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• 한국약용작물학회지
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• 제21권5호
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• pp.394-400
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• 2013

This study was carried out in order to examine the germination characteristics of Atractylodes japonica seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. Experiment was performed by two ways-temperature control (10, 15, 20, $25^{\circ}C$) and shading treatment (full sunlight, 35, 50, 75, 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60 days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 48 hours). Seeds of A. japonica were germinated well under temperature control (overall 85.3~100%), especially high temperature. And, the seeds pre-treated with LTW for 45 days germinated with a 100% success rate. Under the surveying shading treatment, the highest germination rate was 95.8% with DRT and overall 63.2~95.8%. Germination rate under seeds with WS was 63.2~7.8%. As a result of surveying the whole experiment, A. japonica seeds don't need to pre-treat cause of high germination, but if it is performed with LTW for a certain period of time, it would be more productive.

• Mycobiology
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• 제40권2호
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• pp.111-116
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• 2012

Ten strains of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were evaluated to find the most effective strain for optimization studies. The first criterion tested for strain selection was the mortality (> 50%) of Spodoptera litura larvae after inoculation of the fungus for 4 days. Results on several bioassays revealed that B. bassiana BNBCRC showed the most virulence on mortality S. litura larvae (80% mortality). B. bassiana BNBCRC also showed the highest germination rate (72.22%). However, its conidia yield ($7.2{\times}10^8$ conidia/mL) was lower than those of B. bassiana B 14841 ($8.3{\times}10^8$ conidia/mL) and M. anisopliae M6 ($8.2{\times}10^8$ conidia/mL). The highest accumulative radial growth was obtained from the strain B14841 (37.10 mm/day) while the strain BNBCRC showed moderate radial growth (24.40 mm/day). M. anisopliae M6 possessed the highest protease activity (145.00 mU/mL) while M. anisopliae M8 possessed the highest chitinase activity (20.00 mU/mL) during 96~144 hr cultivation. Amongst these criteria, selection based on virulence and germination rate lead to the selection of B. bassiana BNBCRC. B. bassiana B14841 would be selected if based on growth rate while M. anisopliae M6 and M8 possessed the highest enzyme activities.

• 농업생명과학연구
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• 제43권5호
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• pp.1-8
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• 2009

고추 12 품종의 SMP 처리시 carrier와 수분의 배율, 처리기간 및 처리온도에 대한 조사 결과 적정 처리기간과 carrier와 수분의 배율은 품종마다 차이는 있었지만, 처리 중 발아가 일어나지 않았고, 발아율을 높이며, $T_{50}$을 단축시킬 수 있는 처리로 왕과 마니따 품종은 carrier와 수분의 배율이 1:3에서 7일이었다. 나머지 10 품종은 1:7의 비율에서 3일이 좋았다. 종가집 품종은 다른 품종에 비해 처리기간과 수분배율에 따라 발아율과 $T_{50}$이 불규칙한 경향을 나타낸 품종으로 조사되었다. 처리온도는 $15^{\circ}C$에서 $35^{\circ}C$로 설정하여 조사한 결과, 처리 중 유근돌출의 억제와 발아성 향상에 안정적인 처리는 $20^{\circ}C$인 것으로 조사되었다.

• 원예과학기술지
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• 제17권6호
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• pp.747-749
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• 1999

박종자의 발아율 향상과 발아촉진을 위한 종자처리시 hydropriming과 priming 처리와 종자의 발아성을 비교 검토하고, 종자처리 기술의 실용화를 위해 처리 화학물질 및 농도, 처리기간 및 처리온도, 발아온도에 따른 효과를 비교 검토하였다. 증류수(hydropriming)나 50mM $KH_2PO_4$와 50mM $KNO_3$로 $20^{\circ}C$에서 12시간 처리시 $T_{50}$ 및 평균발아소요일수 단축에 효과적 이었다. 무처리종자에 비하여 hydropriming이나 priming에 의한 발아촉진 효과는 발아온도 $25^{\circ}C$나 $35^{\circ}C$보다 $20^{\circ}C$에서 현저하게 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.

• 생명과학회지
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• 제12권6호
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• pp.721-730
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• 2002

본 연구는 파 종자의 발아력을 증진시킬 수 있는 생장조절제 최적 처리조건을 구명하며, 파 종자 펠렛용으로 적합한 접착제 및 피복재료를 선발하기 위해 수행되었다. 식물생장조절제 종자처리는 발아율을 증진시키지는 못했다. 그러나 50% 발아에 소요되는 일수($T_{50}$)와 MDG는 단축되어 조기발아 하였다. 생장조절제 최적 처리조건은 500$\mu$M의 $GA_3$로 1일간 처리였다. 생장조절제 처리에 의한 발아촉진 효과는 발아적온보다는 저온인 $15^{\circ}C$에서 뚜렷하였다. 종자 펠렛의 접착제 종류 및 농도에 따라 발아율에는 큰 차이가 없었으나 발아속도는 대조구 종자에 비해 약 0.3~0.5일 지연 되었다. 종자 펠렛용 접착제의 농도가 증가하면 발아율이 약간 감소하였는데, 이러한 경향은 Carboxymethyl cellulose (CMC) 및 Methyl cellulose(MC)에서 뚜렷하였다. 종자 펠렛 접착제 가운데 Polyvinyl alcohol(PVA)와 Polyvinyl pyrrolidone(PVP)에서는 처리농도가 증가하더라도 발아를 크게 억제하지 않아, 파 펠렛종자 제조에 적용될 수 있는 최적 접착제였다. 펠렛 피복재료 중 kaoline, bentonite ＋ kaoline 혼합물질 및 bentonite ＋ CC ＋ DME #300 혼합물질로 펠렛된 종자에서 높은 발아율과 발아세를 보여 파종자의 펠렛에 적합한 피복재료였다.