• Title/Summary/Keyword: Days to 50% germination rate

Search Result 138, Processing Time 0.039 seconds

Estimation of Cardinal Temperatures for Germination of Seeds from the Common Ice Plant Using Bilinear, Parabolic, and Beta Distribution Models

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • Horticultural Science & Technology
    • /
    • v.34 no.2
    • /
    • pp.236-241
    • /
    • 2016
  • The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

Effect of Light Quality during $GA_3$ Imbibition and Germination Temperature on Pepper Seed Germinability (파종 전 $GA_3$와 광질 처리, 발아온도에 따른 고추종자의 발아율)

  • 강진호;심영도;강신윤;조영욱;박아정
    • Korean Journal of Plant Resources
    • /
    • v.12 no.2
    • /
    • pp.95-101
    • /
    • 1999
  • Higher and uniform germination should be necessary because many commercial pepper (Capsicum annum L.) seedlings were nowadays sold to farmer. The experiment was carried out to determine the effect of its cultivars (Daemyung; Wanggochu), GA$_3$ (concentration; period), light quality (red; far-red; blue; dark) during GA$_3$imbibition, and germination temperature (GT: 25 or 15$^{\circ}C$ constant; 25/15$^{\circ}C$ alternating) on the rate of germination done under incandescent lamps until 9 days after sowing. Final seed germination was not different between GA$_3$0 to 1.0 mM concentrations but the elapsed days to 50% germination $(T_{50})$ were more reduced by GA$_3$ treatment than water imbibition. Under $25^{\circ}C$ constant germination temperature, earlier germination was enhanced by GA$_3$treatment showing the lowest rate at darkness, although the final germination rates of water imbibition and GA$_3$ treatments were same. The final germination rates of alternating and 25 $^{\circ}C$ constant GT in cv. Daemyng was also equal, while the germination rates of $25^{\circ}C$ and 15$^{\circ}C$ constant GT were the highest and the lowest regardless of cultivars. There was no difference between light quality treatments impelled during GA$_3$ imbibition when light treated seeds were germinated at alternating and $25^{\circ}C$ constant GT. At 15$^{\circ}C$ constant GT, however, red light or dark treatment during GA$_3$imbibition increased the germination rate since 5 days after sowing.

  • PDF

Cultivation of Sprout by Highly Concentrated Oxygen Water Soaking (고농도 산소수 침지를 통한 새싹 재배)

  • Cha, Jin-Myeong;Hong, Seung-Ho;Kim, Sun-Yil;Park, Ju-Young;Kim, Maeng-Su;Lee, In-Hwa
    • KSBB Journal
    • /
    • v.23 no.6
    • /
    • pp.525-528
    • /
    • 2008
  • In order to compare the germination and growth rate of the sprouts soaked in highly concentrated oxygen water, it with specific amounts of oxygen dissolved was produced in a high pressure reactor by pressuring oxygen. The sprouts were observed after being soaked in $20^{\circ}C$ oxygen water with 20, 30, 40, 50 ppm of oxygen dissolved each. Results of ten days later indicate that the final germination rate of the sprout soaked in 50 ppm oxygen water was $24.6{\sim}28.6%$ higher than that of the sprout soaked in distilled water. The final growth length also measured 6-7 mm higher than the sprout soaked in distilled water, demonstrating that enough supply of oxygen to the sprout induces stability and efficiency in its growth.

Germination Responses and Early Growth of Allium thunbergii by Temperature and Shading Level (온도와 차광수준에 따른 산부추의 발아반응 및 초기생육)

  • Jeon, Kwon-Seok;Song, Ki-Seon;Choi, Kyu-Seong;Kim, Chang-Hwan;Park, Yong-Bae;Kim, Jong-Jin
    • Journal of Bio-Environment Control
    • /
    • v.24 no.3
    • /
    • pp.178-186
    • /
    • 2015
  • This study was carried out to determine the effects of environment controls (temperature and shading level) on germination responses and early growth of Allium thunbergii. Germination experiment was performed by pre-treatment (with low temperature and wetting treatments for 0, 20, 40 and 60 days) and temperature controls (5, 10, 15, 20, 25 and 30). And growth experiment was performed by containers (128 and 200 cavities containers) and shading level (full sunlight (control), 35%, 50% and 75% shading). Germination rate of A. thunbergii seeds were, 20 days of seed pre-treatment, the highest at $10^{\circ}C$ (81.7%) and the more temperature went up, the more germination rate went down. As a result of surveying container and shading treatments, the height, leaf area, leaf length, leaf aspect ratio (L/W) were higher under 50% shading of 128 (24.2cm, $2.76cm^2$, 22.3cm and 223.4, respectively) and 200 (22.6cm, $2.29cm^2$, 19.4cm and 190.5, respectively) cavities container. The root was grown well under full sunlight. Specially, fresh weight of shoot (leaves+stem) was higher under 50% shading of 128 (0.241g) and 200 (0.212g) cavities container. As a result of surveying the whole experiment, A. thunbergii seeds need to pre-treatment (with low temperature and wetting treatments for 20~40 days) for high germination rate. And it is judged better growth and higher yield by maintaining 50% shading of 200 cavities container.

Effect of Chlorella Culture Solution Using Anaerobic digestate on Seed Germination in Perennial Ryegrass (혐기소화액을 배지로 이용한 클로렐라 배양액 처리가 페레니얼라이그라스 종자 발아에 미치는 영향)

  • Byeon, Ji-Eun;Lee, Jin Woong;Choi, Min Soo;Ryoo, Jong-Won
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.38 no.1
    • /
    • pp.7-15
    • /
    • 2018
  • This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

Evaluation of Crossability, Seed Dormancy and Overwintering Ability in Glufosinate Ammonium-Resistant GM Rice and Their Hybrids with Non-GM and Weedy Rice

  • Lee, Seung-Yeob;Kim, Min-Soo;Kim, Hyo-Jin;Han, Seong-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.51 no.1
    • /
    • pp.53-58
    • /
    • 2006
  • This study was conducted to investigate the crossability, seed dormancy and overwintering ability of rice plant in GM (glufosinate ammonium-resistant lines. Iksan 483 and Milyang 204) and non-GM (their parents) or red rice (Andongaengmi). Seed-setting rate was not significantly different between GM and non-GM rice varieties. Iksan 483 and Milyang 204 showed the similar level of seed germination rate from 30 to 50 days after heading as compared to non-GM rice varieties. After overwintering in paddy field, seed germination rate of GM and non-GM rice varieties ranged from 14.3 % to 57.6 % in dry soil condition, but there was no germination in wet-soil except red rice. The result in wet-soil condition may help to set up a strategy for reducing the risk of gene flow of transgene via dispersal of seeds of GM plants. The crossability, seed dormancy and seed overwintering of Iksan 483 and Milyang 204, herbicide resistant GM rice varieties, were not significantly different compared to non-GM rice varieties. The results might be helpful to reduce the risk of transgene dispersal from GM crop via seeds and pollens.

Effect of Priming and Light Quality on Seed Germintion in Three Campanulan Plants (Priming과 파종후(播種後) 광질처리(光質處理)에 따른 도라지, 더덕, 만삼의 발아율(發芽率))

  • Kang, Jin-Ho;Park, Jin-Seo;Kim, Dong-Il
    • Korean Journal of Medicinal Crop Science
    • /
    • v.5 no.2
    • /
    • pp.139-146
    • /
    • 1997
  • Priming has been used to establish a better standing in practice as controling the seed moisture content after sowing. The experiment was done to measure the effect of priming (material ; concentration ; period) and light quality (red ; white ; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum ; Codonopsis lanceolata ; C. pilosula) to give an information on their earlier standing establishment. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. pilosula, C. lanceolata. Their germination and radicle elongation became more inclined when primed with $Ca(CO_3)_2$ or with 50 to 150mM than with $KNO_3$ or no priming although there was no difference between priming periods. Under irradiation during their seed germination, however, the former order was changed to P. grandiflorum, C. lanceolata, C. pilosula because light quality treatment given after priming reduced the rate of C. pilosula but enhanced that of C. lanceolata. Although light quality forced after priming did not affect the mean germination rate of P. grandiflorum, it increased earlier or alltime germination of C. lanceolata or C. pilosula, respectively. White light after priming repressed germinations of C. lanceolata and C. pilosulai but increased earlier germinatin of P. grandiflorum, meaning that seed germination and radicle elongation of Campanulaceae could be determined by light quality treated after priming.

  • PDF

Technique Development of Wildgrasses for Hydroseeding Material (야생초 가공에 의한 종자분사공법 재료화 기술)

  • 주영규;김성균
    • Asian Journal of Turfgrass Science
    • /
    • v.11 no.3
    • /
    • pp.161-166
    • /
    • 1997
  • This study was performed to develope the method of wildgrass to process as hydroseeding material which is a most popular revegetation method for Slope areas through the control of soil eroison and stability by seeding grasses. The wildgrass material for the processing were harvested at Suwon, Kyungki-do and Wonju, Kangwon-do in 1994. The investigations were established in laboratory for pot and field experiment with seven treatments by harvesting date and place in 1995. Results indicated that wildgrass harvested field in open space or road side was able to be used as hydroseeding material by proper processing. It was possible to substitute wildgrass seed to conrrnercial turfgrass seed, and plant tissue to mulching fiber by drying and crushing process. Germination of wildgrass seed was relatively slower than control (20g /$m^2$ turfgrass seed) in early stage of 20 days, but surface revegetation rate was higher in 50 days at wildgrass plot than control applied by normal hydroseeding method. Mixture of 5g /$m^2$ of cool season turfgrass seed to wildgrass material increased the rate of early stage of germination and surface revegetation. The application of wildgrass as hydroseeding material showed more naturalistic scenery than rutine hydroseeding with imported cool season turfgrass seed. Key words: Rydroseeding, Turfgrass, Wildgrass, Revegetation.

  • PDF

Effects of Morphological Characteristics and Biomass on Germination and Growth of Bulbil for Production of Leaf Garlic (잎마늘 생산을 위한 마늘 주아의 무게 및 형태가 발아와 생장에 미치는 영향)

  • Jeon, Youn A;Cha, Mi-Kyung;Cho, Young-Yeol
    • Journal of Bio-Environment Control
    • /
    • v.26 no.3
    • /
    • pp.221-226
    • /
    • 2017
  • The objective of this study was to find out the most optimal bulbil size and planting densities of garlic bulbils to germination for production of leaf garlic. In the first this experiment, the bulbils classified by the size of bulbil to Big (>0.2) and Small (${\leq}0.2$), were planted at $13,680bulbils/m^2$ and $18,240bulbils/m^2$ (Big), $18,240bulbils/m^2$ and $22,800bulbils/m^2$ (Small), respectively. The germination rate, plant height and total yield were measured. In the second experiment, length, width, fresh weight and dry weight of 200 garlic bulbils were measured to analyze the correlation of days to first germination, T50 and germination rate. In the first experiment, the germination rate showed the highest at Big bulbils. The density of $18,240bulbils/m^2$ was the most suitable for cultivation because of the highest germination rate. In the case of yield, it was found that both of the fresh weight and the dry weight of Big bulbil was 2.8 times heavier than Small bulbil. Therefore, Big bulbil with the density of $18,240bulbils/m^2$ was the most suitable for cultivation because of the highest yield. The correlation between each factor of morphological characteristics had a positive correlation between fresh weight, dry weight and width. Also, the germination rate had a positive correlation with fresh weight and width. And, days to first germination and T50 had a negative correlation with fresh weight and width. In conclusion, the optimal planting density is $18,240bulbils/m^2$ and the optimal size of bulbil was 0.2g or more. However, thicker and heavier bulbils could lead to higher and faster germination.

Gamma Radiation Sensitivity and Quantitative Characters in M1 Generation of Heteropappus hispidus (Thunb.) (갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징)

  • Oh, Byung-Kwon;Hong, Kyung-Ae;Song, Sung-Jun;Lee, Sun-Joo;Lee, Young-Il;U., Zang-Kual
    • Korean Journal of Environmental Agriculture
    • /
    • v.22 no.4
    • /
    • pp.294-299
    • /
    • 2003
  • The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy) of gamma rays to investigate their germination rate and to quantity the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120Gy was not greatly affected but was rapidly decreased at over 160Gy. It seemed that lethal dose ($LD_{50}$) of germination was 160Gy. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40Gy. Multi-shoots were developed over 80Gy. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).