• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.275-286
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• 2014

To successful molecular breeding, identification and functional characterization of breeding related genes and development of molecular breeding techniques using DNA markers are essential. Although the development of a useful marker is difficult in the aspect of time, cost and effort, many markers are being developed to be used in molecular breeding and developed markers have been used in many fields. Single nucleotide polymorphisms (SNPs) markers were widely used for genomic research and breeding, but has hardly been validated for screening functional genes in olive flounder. We identified single nucleotide polymorphisms (SNPs) from expressed sequence tag (EST) database in olive flounder; out of a total 4,327 ESTs, 693 contigs and 514 SNPs were detected in total EST, and these substitutions include 297 transitions and 217 transversions. As a result, 144 SNP markers were developed on the basis of 514 SNP to selection of useful gene region, and then applied to each of eight wild and culture olive flounder (total 16 samples). In our experimental result, only 32 markers had detected polymorphism in sample, also identified 21 transitions and 11 transversions, whereas indel was not detected in polymorphic SNPs. Heterozygosity of wild and cultured olive flounder using the 32 SNP markers is 0.34 and 0.29, respectively. In conclusion, we identified SNP and polymorphism in olive flounder using newly designed marker, it supports that developed markers are suitable for SNP detection and diversity analysis in olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

• 한국산학기술학회논문지
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• 제13권5호
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• pp.1976-1982
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• 2012

압축기는 다양한 유체기계에 널리 사용되고 있으며, 특히 압축기의 성능은 냉동공조 시스템의 성능계수에 직접적인 영향을 미치므로 압축기의 효율을 높이기 위한 노력이 계속되어 왔다. 용도에 맞게 다양한 종류의 압축기가 개발되었고, 그 중 스크롤 압축기는 그 크기에 비하여 압축 성능이 우수하여 여러 분야에서 선호하는 압축기이다. 스크롤 압축기의 개발을 위하여 실험적인 연구는 많이 수행되어 온 반면, 개발 목적에 맞게 압축기를 해석할 수 있는 모델은 많지 않은 실정이다. 따라서 본 연구에서는 스크롤 압축기를 기하학적으로 모델링하여 해석할 수 있는 모델을 1-D 상용 프로그램인 AMESim을 이용하여 개발하였으며, 실험 데이터를 이용하여 모델의 신뢰성을 검증하였다. 본 연구 결과는 추후 스크롤 압축기를 개발하는 과정에서 압축기의 성능을 예상하고 최적화 할 수 있는 방법을 제공할 것으로 기대된다.

• 한국항공우주학회지
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• 제33권1호
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• pp.104-109
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• 2005

항공기용 가스터빈엔진에 사용되고 있는 전자식 엔진제어장치는 연료 절약 및 성능 향상을 목적으로 개발되어, 최근에는 엔진의 신뢰성 향상을 위한 고장진단 기능을 갖춘 전자동디지털식 엔진제어장치로 발전하였다. 이와 같은 기술의 발전으로 과거와는 다른 새로운 개념의 엔진제어장치에 대한 인증기술 개발의 필요성이 증대되고 있다. 전자식 엔진제어장치에 대한 설계 및 인증에서 고려되어야 하는 사항으로 전원 공급, 입력 데이터, 고장 모드, 소프트웨어 확인 및 검증, 그리고 낙뢰의 영향 등을 들 수 있다. 본 연구에서는 전자식 엔진제어장치에 대한 설계 고려사항과 인증 기법을 분석하였고, 이를 바탕으로 국내 감항기술기준의 관련 요구조건을 정립할 수 있도록 하였다.

• 방송공학회논문지
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• 제5권1호
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• pp.82-93
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• 2000

최근 디지털 비디오 데이터가 급격히 증가하고 대중화됨에 따라 이를 활용하기 위한 효율적인 접근 기법이 절실히 요구되고 있다. 비디오 요약(video summarization) 기법은 의미적으로 중요한 요점만으로 전체 비디오를 표현하는 것으로 비디오 내용에 대한 전반적인 개관(overview)을 제공할 뿐만 아니라 브라우징(browsing) 등의 유용한 접근 기능을 제공한다. 본 논문에서는 의미적으로 중요한 내용을 포함하는 비디오 주요구간(highlight segment) 검출을 통한 새로운 동적 요약(dynamic summarization) 기법과 생성된 요약 정보 표현을 위하여 MPEG-7에 제안한 요약 기술구조(DS : Description Scheme)에 대하여 기술한다. 본 논문의 기술구조는 다중 계층의 하이라이트(highlight), 계층적 브라우징, 사용자 주문형 요약 등의 기능을 통하여 비디오의 개관 및 효율적인 브라우징, 네비게이션(navigation)을 가능하게 한다. 또한, 제안하는 비디오 요약 기법 및 요약 기술구조의 실현 가능성 및 기능 구현을 확인하기 위하여 축구 비디오에 대한 적용 실례를 제시한다.

• 한국항공우주학회지
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• 제43권3호
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• pp.282-287
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• 2015

현대전에서 적 방공망 제압 임무는 과거에 비해 그 중요성이 점차 부각되고 있다. 그러나 본 임무는 적의 방공망에 아군의 항공기와 조종사가 노출되는 위험을 안고 있어, 아군의 피해를 최소화하면서 효과적으로 임무를 수행할 수 있도록 하는 연구가 활발히 진행되고 있다. 본 논문에서는 이러한 연구의 일환으로써, 벡터필드 유도기법을 이용하여, 다수 무인기의 적 방공망 제압 임무를 설계하였다. 벡터필드 유도기법은 다른 유도기법들에 비하여 계산 량이 적고 쉽게 응용이 가능한 이점이 있어, 다수 무인기 운용에 해당 기법을 적용하는 경우 무인기간의 상호 협동을 통한 다양한 형태의 임무를 계획할 수 있다. 따라서 벡터필드 유도기법을 이용한 다수 무인기의 적 방공망 제압 임무의 설계방법을 제시함과 동시에, 비행실험을 통하여 그 운용 가능성을 확인해 보았다.

• 한국농공학회논문집
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• 제60권4호
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• pp.49-54
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• 2018

With increasing satellite-based RS(Remotely Sensed) techniques, RS soil moisture footprints have been providing for various purposes at the spatio-temporal scales in hydrology, agriculture, etc. However, their coarse resolutions still limit the applicability of RS soil moisture to field regions. To overcome these drawbacks, the LDA(Landsat-based Downscaling Algorithm) was developed to downscale RS soil moisture footprints from the coarse- to finer-scales. LDA estimates Landsat-based soil moisture($30m{\times}30m$) values in a spatial domain, and then the weighting values based on the Landsat-based soil moisture estimates were derived at the finer-scale. Then, the coarse-scale RS soil moisture footprints can be downscaled based on the derived weighting values. The LW21(Little Washita) site in Oklahoma(USA) was selected to validate the LDA scheme. In-situ soil moisture data measured at the multiple sampling locations that can reprent the airborne sensing ESTAR(Electronically Scanned Thinned Array Radiometer, $800m{\times}800m$) scale were available at the LW21 site. LDA downscaled the ESTAR soil moisture products, and the downscaled values were validated with the in-situ measurements. The soil moisture values downscaled from ESTAR were identified well with the in-situ measurements, although uncertainties exist. Furthermore, the SMAP(Soil Moisture Active & Passive, $9km{\times}9km$) soil moisture products were downscaled by the LDA. Although the validation works have limitations at the SMAP scale, the downscaled soil moisture values can represent the land surface condition. Thus, the LDA scheme can downscale RS soil moisture products with easy application and be helpful for efficient water management plans in hydrology, agriculture, environment, etc. at field regions.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• 한국축산시설환경학회지
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• 제20권3호
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• pp.117-124
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• 2014

The objectives were to assess the use of near-infrared reflectance spectroscopy (NIRS) as a tool for estimating nutrient compositions of corn kernel, and to apply an NIRS-based indium gallium arsenide array detector to the system for collecting spectra and analyzing calibration equations using equipments designed for field application. Partial Least Squares Regression (PLSR) was employed to develop calibration equations based on representative data sets. The kennard-stone algorithm was applied to induce a calibration set and a validation set. As a result, the method for structuring a calibration set significantly affected prediction accuracy. The prediction of chemical composition of corn kernel resulted in the following (kennard-stone algorithm: relative) moisture ($R^2=0.82$, RMSEP=0.183), crude protein ($R^2=0.80$, RMSEP=0.142), crude fat ($R^2=0.84$, RMSEP=0.098), crude fiber ($R^2=0.74$, RMSEP=0.098), and crude ash ($R^2=0.81$, RMSEP=0.048). Result of this experiment showed the potential of NIRS to predict the chemical composition of corn kernel.

• Food Science and Biotechnology
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• 제14권1호
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• pp.42-45
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• 2005

The present study was carried out to envisage the aerobic growth of Listeria monocytogenes and Yersinia enterocolitica on pork, which is one of the major meat sources in Korea. The results were compared with the previously developed predictive model systems for the verification of microbial growth in a real situation during pork processing. Pork loin samples (8.0 g, 5 mm thick) were aseptically prepared and inoculated with each pathogen by immersing into the respective inoculums for one min. Each of the samples were then wrapped with PE film and stored at 5, 10, and $15^{\circ}C$ up to 36 days to measure the growth profile of the respective pathogens. The growth parameters were calculated by using Gompertz equation and were compared with the previously reported data. The predicted generation time (GT) of L. monocytogenes at 5, 10 and $15^{\circ}C$ was 28.74, 7.85 and 4.02 hr, respectively, and for Y. enterocolitica was 10.29, 4.74 and 2.50 hr, at the same temperatures respectively. In this study, the GT values predicted on pork were slightly higher than the values predicted in other studies using liquid model systems. Unlike previous reports, both the pathogens were found to grow at $5^{\circ}C$ on pork. This finding recommends the necessity of controlling the growth of both the pathogens during the slaughtering process and distribution.

• 대기
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• 제26권2호
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• pp.277-288
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• 2016

Polar lows are intense mesoscale cyclones that mainly occur over the sea in polar regions. Owing to their small spatial scale of a diameter less than 1000 km, simulating polar lows is a challenging task. At King Sejong station in West Antartica, polar lows are often observed. Despite the recent significant climatic changes observed over West Antarctica, adequate validation of regional simulations of extreme weather events such as polar lows are rare for this region. To address this gap, simulation results from a recent version of the Polar Weather Research and Forecasting model (Polar WRF) covering Antartic Peninsula at a high horizontal resolution of 3 km are validated against near-surface meteorological observations. We selected a case of high wind speed event on 7 January 2013 recorded at Automatic Meteorological Observation Station (AMOS) in King Sejong station, Antarctica. It is revealed by in situ observations, numerical weather prediction, and reanalysis fields that the synoptic and mesoscale environment of the strong wind event was due to the passage of a strong mesoscale polar low of center pressure 950 hPa. Verifying model results from 3 km grid resolution simulation against AMOS observation showed that high skill in simulating wind speed and surface pressure with a bias of $-1.1m\;s^{-1}$ and -1.2 hPa, respectively. Our evaluation suggests that the Polar WRF can be used as a useful dynamic downscaling tool for the simulation of Antartic weather systems and the near-surface meteorological instruments installed in King Sejong station can provide invaluable data for polar low studies over West Antartica.