Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Simultaneous Analysis of Phenolic Compounds in Geranium thunbergii Using UPLC

페놀성 화합물을 이용한 현지초의 UPLC 다성분 동시분석 개발

  • Kim, Se-Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lamichhane, Ramakanta (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, College of Pharmacy, Wonkwang University) ;
  • Lee, Kyung-Hee (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, College of Pharmacy, Wonkwang University) ;
  • Pandeya, Prakash Raj (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, College of Pharmacy, Wonkwang University) ;
  • Shim, Sang-Yeon (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, College of Pharmacy, Wonkwang University) ;
  • Jung, Hyun-Ju (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, College of Pharmacy, Wonkwang University)
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 라미차네 라마칸타 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 이경희 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 판데야 프라카스 라즈 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 심상연 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 정현주 (원광대학교 약학대학 한약학과 및 원광한약연구소)
  • Received : 2018.01.15
  • Accepted : 2018.02.23
  • Published : 2018.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to develop a UPLC method for simultaneous analysis of 8 phenolic compounds including gallic aicd (1), protocatechuic acid (2), methyl gallate (3), ellagic acid (4), kaempferol-3-arabinofranosyl-7-rhamnoside (5), kaempferitrin (6), afzelin (7) and kaempferol-7-rhamnoside (8) isolated from Geranium thunbergii which has been traditionally used as anti-diarrheal agent. The UPLC method was optimized and validated using Halo C18 column ($4.6{\times}100mm$, $2.7{\mu}m$) consisting of MeOH and 0.1% formic acid at 260 nm in 25 minutes. In quantitative analysis of 8 compounds in MeOH extract of G. thunbergii, contents of 4-6 were 12.39, 20.52 and 21.45 mg/g, respectively. These compounds were measured as major phenolic compounds in G. thunbergii and can be useful as marker compounds for its quality control. These results suggest that the UPLC method can be contributed as basic data for quality evaluation of herbal preparations.

Keywords

References

  1. Sung, M. S., Jung, H. Y., Choi, J. H. Lee, S. C. Choi, B. H. and Park, S.S. (2014) Preparation of functional healthy drinks by Acanthopanax senticosus extracts. J. Life. Sci. 24: 959-966. https://doi.org/10.5352/JLS.2014.24.9.959
  2. Liu, Q. H., Jeong, J. E., Choi, E. J., Moon, Y. H. and Woo, E. R. (2006) A new furofuran lignan from Geranium thunbergii Sieb. et Zucc. Arch. Pharm. Res. 29: 1109-1113. https://doi.org/10.1007/BF02969300
  3. Pokharel, Y. R., Liu, Q. H., Oh, J. W., Woo, E. R. and Kang, K. W. (2007) 4-Hydroxy-kobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB and AP-1 activation. Biol. Pharm. Bull. 30: 1097-1101. https://doi.org/10.1248/bpb.30.1097
  4. Takuo, O., Takashi, Y. and Kazuko. M. (1975) Brevifolin, corilagin and other phenols from Geranium thunbergii. Phytochemistry 14: 1877-1878. https://doi.org/10.1016/0031-9422(75)85321-0
  5. Takuo, O., Kazuko, M., Kaoru, S. and Tsutomu, H. (1979) Constituents of Geranium thunbergii Sieb. et Zucc.: VII. High-Performance reversed-phase liquid chromatography of hydrolysable tannins and related polyphenols. J. Chromatogr. A. 171: 313-320. https://doi.org/10.1016/S0021-9673(01)95310-5
  6. Wu, Q. Y., Zhou, Y., Jin, X., Guan, Y., Xu, M. and Liu, L. F. (2011) Chromatographic fingerprint and the simultaneous determination of five bioactive components of Geranium carolinianum L. water extract by high performance liquid chromatography. Int. J. Mol. Sci. 12: 8740-8749.
  7. Li, F., Jin, X., Yao, L., Dong, C. X., Gong, Y. J. and Liu, L. F. (2010) Study on the dynamic variation of active components in Geranium carolinianum from different collection time. Zhong Yao Cai 33: 1545-1548.
  8. Liu, D., Su, Z., Wang, C., Gu, M. and Xing, S. (2010) Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography. J. Sep. Sci. 33: 2266-2271. https://doi.org/10.1002/jssc.200900599
  9. Kim, O. S., Seo, C. S. and Shin, H. K. (2013) Simultaneous determination of Gumiganghwal-tang and its anti-atherosclerotic effect. Kor. J. Orient. Pre. Med. Soc. 17: 165-176.
  10. Seo, C. S., Kim, O. S. and Shin, H. K. (2014) Quantitative determination and antioxidant effects of Cheonwangbosimdan. Kor. J. Pharmacogn. 45: 300-314.
  11. Kwon, Y. J., Jang, G. C., Rha, H. H., Kim, Y. H. and Rhee, M. S. (2005) The study of sugar analysis in licorice extract by HPLC. J. Kor. Soc. Tobacco. Sci. 27: 114-119.
  12. Ko, J,, Lee, M. S., Kwak, B. M., Ahn, J. H., Park, J. S. and Kwon, J. H. (2013) Determination of isomaltooligosaccharides in yoghurts by using HPLC-ELSD. Korean. J. Food. Sci. An. 33: 417-424. https://doi.org/10.5851/kosfa.2013.33.3.417
  13. Choi, S. J., Kim, J. K., Jang, J. M. and Lim, S. S. (2012) Inhibitory effect of the phenolic compounds from Geranium thunbergii on rat lens aldose reductase and galactitol formation. Korean J. Medicinal Crop Sci. 20: 222-230 https://doi.org/10.7783/KJMCS.2012.20.4.222
  14. Youn, K. and Jun, M. (2013) In vitro BACE1 inhibitory activity of geraniin and corilagin from Geranium thunbergii. Planta Med. 79: 1038-1042. https://doi.org/10.1055/s-0032-1328769
  15. Sohretoqlu, D., Sabuncuoqlu, S. A., Sakar, M. K., Ozgunes, H. and Sterner, O. (2010) Antioxidant effects of secondary metabolites from Geranium psilostemon. Nat. Prod. Commun. 5: 899-902.
  16. Lu, C. H., Li, Y. Y., Li, L. J., Liang, L. Y. and Shen, Y. M. (2012) Anti-inflammatory activities of fractions from Geranium nepalense and related polyphenols. Drug Discov. Ther. 6: 194-197.
  17. Kim, Y., Han, S. H., Jeon, J. Y., Hwang, M. H., Im, Y. J., Lee, S. Y., Chae, S. W. and Kim, M. G. (2013) Validation of LCMS/ MS method for determination of ginsenoside Rg1 in human plasma. Anal. Sci. Technol. 26: 221-227. https://doi.org/10.5806/AST.2013.26.4.221