• Title/Summary/Keyword: Data integrity

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Application of Multiparametric Flow Cytometry (FCM) to Enumerate the Diagnosis of Pseudomonas aeruginosa and Escherichia coli

  • Hwang, Myoung-Goo;Oh, Jung-Woo;Katayama, Hiroyuki;Ohgaki, Shinichiro;Cho, Jin-Kyu
    • Environmental Engineering Research
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    • v.17 no.1
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    • pp.35-39
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    • 2012
  • In this study, multiparametric flow cytometry (FCM) was installed to enumerate the diagnosis of Pseudomonas aeruginosa ATCC 10145 and Escherichia coli K12 (IFO 3301). The nucleic acids (DNA/RNA) were double stained by a LIVE/DEAD bacLight viability kit, involving green SYTO 9 and red propidium iodide (PI), based on the permeability of two chemicals according to the integrity of plasma membrane. As the results showed, the gate for dead bacteria was defined as the range of $0.2{\times}10^0$ to $6.0{\times}10^1$ photo multiplier tube (PMT) 2 fluorescence (X-axis) and $2.0{\times}10^0$ to $2.0{\times}10^2$ PMT 4 fluorescence (Y-axis), and the gate for live bacteria was defined as the range of $6.0{\times}10^0$ to $6.0{\times}10^2$ PMT 2 fluorescence (X-axis) and $2.0{\times}10^0$ to $4.0{\times}10^2$ PMT 4 fluorescence (Y-axis). In the comparison of the number of the tested bacteria detected by FCM (viability assessment) and plate culture (cultivability assessment), the number of bacteria detected by FCM well represented the number of bacteria that was detected by the colony forming unit (CFU) counting method when bacteria were exposed to isopropyl alcohol and silver/copper cations. Consequently, it is concluded that the application of FCM to monitor the functional effect of disinfectants on the physiological status of target bacteria can offer more rapid and reliable data than the plate culture colony counting method.

Effect of supplementation of feed with Flaxseed (Linumusitatisimum) oil on libido and semen quality of Nilli-Ravi buffalo bulls

  • Shah, Syed Mazhar Hussain;Ali, Shujait;Zubair, Muhammad;Jamil, Huma;Ahmad, Nazir
    • Journal of Animal Science and Technology
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    • v.58 no.7
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    • pp.25.1-25.6
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    • 2016
  • Background: The current study was designed to investigate the effect of supplementation of Flaxseed (Linumusitatisimum) oil on libido and semen quality of Nilli-Ravi buffalo bulls. Methods: In this study, 12 adult healthy bulls kept at the Semen Production Unit, Qadirabad district Sahiwal, were used. These bulls were divided into three equal groups, A, B and C. Group A was kept as control, while in groups B and C supplementation of feed was provided by using flaxseed oil @125 ml/day and 250 ml/day,respectively for 12 weeks. Two ejaculates per animal were collected at 0 day then 5th, 6th, 7th, 8th, 9th, 10th, 11th and 12th week of treatment. In this way a total 216 samples were taken, and each semen sample was evaluated for color, volume, mass activity, percent motility, sperm cell concentration per ml, percentage of live sperm, and plasma membrane integrity. Libido of bulls was also evaluated before every collection. Results: Analysis of data revealed that these parameters were significantly (P < 0.01) increased in flax oil treated animals as compared to control. Conclusion: It was concluded from the present study that flax seed oil has beneficial effects on reproductive health of buffalo bull.

UDRE Monitoring Analysis of Korean Satellite Navigation System (한국형 위성항법시스템의 UDRE 모니터링 분석)

  • Park, Jong-Geun;Ahn, Jongsun;Heo, Moon-Beom;Joo, Jung Min;Lee, Kihoon;Sung, Sangkyung;Lee, Young Jae
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.43 no.2
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    • pp.125-132
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    • 2015
  • This paper is about analysis of UDRE monitoring method for Korean Satellite navigation system, which is the correction parameter of satellite measurements. New receiver clock bias and tropospheric delay error estimation method to make pseudo-range residual for UDRE monitoring is proposed. Saastamoinen model and Neill mapping function are used for estimate the tropospheric delay and EKF is used for estimgate the receiver clock bias. Through the satellite measurements and regional weather data received directly from the domestic is using for UDRE monitoring analysis, more suitable UDRE monitoring threshold can be deducted and it is expected to be utilized for fault detection technique of Korean Satellite Navigation System.

A study on the present condition and development direction of China's Agricultural Tourism - Centered on Takgeuktak County of Inner Mongolia Autonomous Region in Particular - (중국(中國) 관광농업(觀光農業)의 현황(現況) 및 발전(發展) 방향(方向) - 내몽고(內蒙古) 자치구(自治區) 탁극탁현(托克托縣)을 중심(中心)으로 -)

  • Park, Jae-Chul;Xu, Ying-Mei;Jung, Kyoung-Sook
    • Journal of Korean Society of Rural Planning
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    • v.19 no.4
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    • pp.213-226
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    • 2013
  • Agricultural tourism is a new kind of industry, which combined agriculture with tourism. It not only has the productive function, but also has the function of improving the quality of ecological environment and supplying people with sightseeing, leisure, vacation. Agricultural tourism is a new kind of industry from the amalgamation of agriculture and tourism: it is based on farm work, characterized by agricultural management, combines agriculture and tourism, and includes the city as a market-place. It satisfies tourist demand with food, lodging, excursions, shopping and entertainment provided by agricultural places and agricultural products. After combining the substance of this study with the primary sources regarding the development of agricultural tourism, I suggest plans for the development of local agricultural tourism on the basis of sustainable tourism theory in case of the Inner Mongolia region. To conduct research, various data such as literature research and surveys are collected and analyzed. According to the result of the research, the Inner Mongolia region has great-wealthy resources for agricultural tourism. However, they have problems as well such as lack of funds, low infrastructure, low business-mind level, low management-mind, the scarcity of mid-supporting organizations for research and education, low awareness, and low service level of the agricultural tourism. In order to solve these problems, have to strengthen publicity, strengthen the county and city communication, educates continuously profession talented person, establish related laws and regulations, increase the integrity of infrastructure. As a developing industry, agricultural tourism is a new field of study which needs more encouraging research. The agricultural tourism of Inner Mongolia Autonomous Region has only just started development resulting in a limited amount of information available for this study. Future planned work entails a continuous in-depth study of Chinese agricultural tourism, considering Inner Mongolia Autonomous Region in particular.

Study on Safety Design of Vertical-Type Heat Recovery Steam Generator Based on Large-Scale Analysis (대규모해석을 활용한 수직형 배열회수 증기발생기의 안전설계에 관한 연구)

  • Ryu, Tae-Young;Yang, Sang-Mo;Jang, Hyun-Min;Choi, Jae-Boong;Myung, Ki-Chul;Lee, Dong-Yun;Choi, Shin-Beom
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.12
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    • pp.1535-1542
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    • 2012
  • A Heat Recovery Steam Generator(HRSG) is the main component of a Combined Cycle Power Plant(CCPP). It is a very large structure that is made from relatively thin metal sheets. Therefore, the structural integrity of an HRSG is very important to ensure safe operation during plant lifetime. In particular, thermal deformation and thermal fatigue have been revealed as the main causes of the mechanical degradation of an HRSG. In order to prevent unexpected damage, safety evaluation based on a large-scale analysis is necessary. Therefore, this study aims to improve the safety of HRSG by using Finite Element Analysis(FEA) results derived from large-scale analysis. Furthermore, the modified design is verified by comparing it with the original one. This result will be used as basic data for improving the safety of a vertical-type HRSG.

Security Analysis of the Whirlpool Hash Function in the Cloud of Things

  • Li, Wei;Gao, Zhiyong;Gu, Dawu;Ge, Chenyu;Liao, Linfeng;Zhou, Zhihong;Liu, Ya;Liu, Zhiqiang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.1
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    • pp.536-551
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    • 2017
  • With the advancement and deployment of leading-edge telecommunication technologies for sensing and collecting, computing related information, Cloud of Things (CoTs) has emerged as a typical application platform that is envisioned to revolutionize the daily activities of human society, such as intelligent transportation, modern logistics, food safety, environmental monitoring, etc. To avoid any possible malicious attack and resource abuse, employing hash functions is widely recognized as one of the most effective approaches for CoTs to achieve message integrity and data authentication. The Whirlpool hash function has served as part of the joint ISO/IEC 10118-3 International Standard by the International Organization for Standardization (ISO) and the International Electrotechnical Commission (IEC). In this paper, we propose an effective differential fault analysis on Whirlpool in the byte-oriented random fault model. The mathematical analysis and experimental results show that 8 random faults on average are required to obtain the current 512-bit message input of whirlpool and the secret key of HMAC-Whirlpool. Our work demonstrates that Whirlpool and HMAC-Whirlpool are both vulnerable to the single byte differential fault analysis. It provides a new reference for the security analysis of the same structure of the hash functions in the CoTs.

Various Applications of Deep Inferior Epigastric Artery Perforator Free Flap (심부하복벽천공지 유리피판의 다양한 임상적 적용)

  • Ahn, Hee Chang;Yang, Eun Zin;Kim, Chang Yeon;Lee, Jang Hyun
    • Archives of Plastic Surgery
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    • v.36 no.6
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    • pp.707-713
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    • 2009
  • Purpose: The deep inferior epigastric perforator(DIEP) free flap is well known as an ideal donor site for the breast reconstruction. The flap can provide huge amount of fat tissue for breast and buttock contour, while it is also very useful as a thin skin flap to reconstruct the upper and lower extremities. We used a DIEP free flap in various site reconstructions besides the breast and would like to reinsure the usefulness of this flap. Methods: Twenty nine consecutive patients who underwent DIEP free flap surgery from 2001 January to 2007 December were reviewed. The case constituted seven male patients and twenty two female patients. There were sixteen breast reconstructions, five face reconstructions, five lower extremity reconstructions, two upper extremity reconstructions, and one buttock contour reconstruction. All clinical data were based on the patient's medical records. Results: All DIEP free flaps survived without major complications. There was no hematoma, seroma, or partial necrosis. The donor sites were closed primarily with linear scar on lower abdomen. The thinnest part of flap was 0.7 cm in thickness. The size of the largest flap was $38{\times}13cm$. The flaps were used in various types of skin and adipose tissue, adipose tissue only, and skin only according to the requirement of recipient site. Conclusion: The DIEP free flap was enough to provide a thin and huge flap for both breast and extremity reconstructions. It was able to provide versatile designs with sufficient adipose tissue. So we use it for 3 - dimentional face and buttock contour reconstructions. The DIEP free flap is a valuable reconstructive donor for face, upper and lower extremity in addition to breast without compromising the integrity of abdominal wall.

Loading Rates and Characteristics of Litter from Highway Stormwater Runoff (강우로 인해 고속도로로부터 유출되는 폐기물의 성상, 부하량 및 유출 특성)

  • Kim, Lee-Hyung;Kang, Joohyon
    • Journal of Korean Society on Water Environment
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    • v.20 no.5
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    • pp.415-421
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    • 2004
  • Litter wastes on highway runoff are gradually being considered one of the major pollutants of concern in protecting the integrity of receiving waters for beneficial use. The California State Water Resources Control Board has identified in their 303(d) list at least 36 water bodies where trash or litter is considered a pollutant of concern. The first TMDL adopted by the Region 4 (Los Angeles area) of the California State Water Quality Control Board was for trash in the Los Angeles River. The first flush characteristic study was developed to obtain first flush water quality and litter data from representative stormwater runoff from standard highway drainage outfalls in the Los Angeles area. Total captured gross pollutants in stormwater runoff were monitored at six Southern California highway sites over two years. The gross pollutants were 90% vegetation and 10% litter. Approximately 50% of the litter was composed of biodegradable materials. The event mean concentrations show an increasing trend with antecedent dry days and a decreasing trend with total runoff volume or total rainfall. Event mean concentrations were ranged 0.0021 to 0.259g/L for wet gross pollutants and 0.0001 to 0.027g/L for wet litters. The first flush phenomenon was evaluated and the impacts of various parameters such as rainfall intensity, drainage area, peak flow rate, and antecedent dry period on litter volume and loading rates were evaluated. First flush phenomenon was generally observed for litter concentrations, but was not apparent with litter mass loading rates. Litter volume and loading rates appear to be directly related to peak storm intensity, antecedent dry days and total flow volume.

FIV Analysis of SG Tubes for Various TSP Locations (튜브 지지판 재배치에 따른 유체유발진동 특성 해석)

  • Kim, Hyung-Jin;Park, Chi-Yong;Park, Myoung-Ho;Ryu, Ki-Whan
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.9 s.102
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    • pp.1009-1015
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    • 2005
  • Fluid-elastic instability and turbulence excitation for an under developing steam generator are investigated numerically. The stability ratio and the amplitude of turbulence excitation are obtained by using the $PIAT^{(R)}$ (program for integrity assessment of steam generator tube) code from the information on the thermal-hydraulic data of the steam generator. The aspect ratio, the ratio between the height of U-tube from the upper most tube support Plate (h) and the width of two vertical portion of U-tube (w), is defined for geometric parameter study. Several aspect ratios with relocation of tube support plates are adopted to study the effects on the mode shapes and characteristics of flow-induced vibration. When the aspect ratio exceeds value of 1, most of the mode shapes at low frequency are generated at the top of U-tube. It makes very high value of the stability ratio and the amplitude of turbulent excitation as well. We can consider that the local mode shape at the upper side of U-tube will develop the wear phenomena between the tube and the anti-nitration bars such as vortical, horizontal, and diagonal strips. It turns out that the aspect ratio reveals very important parameter for the design stage of the steam generator. The appropriate value of the aspect ratio should be specified and applied.

Current Status on the Development and Application of Fatigue Monitoring System for Nuclear Power Plants (원전 피로 감시 시스템 개발 및 적용 현황)

  • Boo, Myung Hwan;Lee, Kyoung Soo;Oh, Chang Kyun;Kim, Hyun Su
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.13 no.2
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    • pp.1-18
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    • 2017
  • Metal fatigue is an important aging mechanism that material characteristics can be deteriorated when even a small load is applied repeatedly. An accurate fatigue evaluation is very important for component structural integrity and reliability. In the design stage of a nuclear power plant, the fatigue evaluations of the Class 1 components have to be performed. However, operating experience shows that the design evaluation can be very conservative due to conservatism in the transient severity and number of occurrence. Therefore, the fatigue monitoring system has been considered as a practical mean to ensure safe operation of the nuclear power plants. The fatigue monitoring system can quantify accumulated fatigue damage up to date for various plant conditions. The purpose of this paper is to describe the fatigue monitoring procedure and to introduce the fatigue monitoring program developed by the authors. The feasibility of the fatigue monitoring program is demonstrated by comparing with the actual operating data and finite element analysis results.