• Title/Summary/Keyword: Dark treatment

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Effect of Light and Cadmium on the Activity and Isozyme Pattern of Catalase from Ric(Oryza sativa L.) (빛과 카드뮴이 벼 catalase 활성과 동위효소 발현에 미치는 영향)

  • Kim, Yoon-Kyoung;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.287-292
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    • 2006
  • The effects of cadmium on the catalase activity and isozyme patterns under light and dark conditions of rice(Oryza sativa L. cv. Dongjin) seedlings were examined. Cadmium treatment resulted in the notable enhancement of $H_2O_2$ contents in the seedling roots and leaves under light and dark conditions. The catalase isozyme patterns in the roots were different from those in the leaves, showing tissue-specific expression of the enzyme. Moreover, the expression patterns of catalase isozymes in the green seedling roots were different from those in the etiolated seedling roots following cadmium treatment. The increase of total catalase activity was about 16 times at 1 mM cadmium and marked inductions of the isozyme CAT1 and CAT2 contributed to this increase in the green seedling roots. On the other hand, in the etiolated seedling roots, total catalase activity was lower than that of control at 0.5 and 1 mM cadmium, even though catalase activity increased about 3 times at 0.1 mM cadmium. The 3 fold increase of total catalase activity was mainly due to the increase of CAT1, CAT3 and CAT4 at 0.1 mM cadmium. However, treatment with higher concentrations of cadmium decreased the activity of CAT2 and CAT4 in the etiolated roots. In the leaves, the catalase existed as three isozymes; one cationic isozyme CATc, one neutral isozyme CATn and one anionic isozyme CAT1 in the control. The isozyme patterns and total activities remained unaffected by cadmium under light and dark conditions in the seedling leaves. Taken together, it seems that cadmium-induced changes of catalase might be regulated by light in the roots, but not in the leaves.

Rancidity Analysis of Rapeseed Oil under Different Storage Conditions Using Mass Spectrometry-based Electronic Nose (질량분석기 기반-전자코를 이용한 저장중 유채유의 산패 분석)

  • Hong, Eun-Jeung;Lim, Chae-Lan;Son, Hee-Jin;Choi, Jin-Young;Noh, Bong-Soo
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.699-704
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    • 2010
  • Rapeseed oil was stored under different conditions such as in the dark, with UV treatment, and with prooxidantscytochrome C and copper ion. The rapeseed oils stored at different temperatures were analyzed by a mass spectrometrybased electronic nose and discriminant function analysis (DFA). Volatile components in the rapeseed oil increased with storage time, and the discriminant function first score (DF1) moved from a positive position to a negative position as storage time increased. Changes in DF1 were higher under UV treatment than under the dark condition (DF1: $r^2$=0.9481, F=307.03). The different DF1 values (F1) under the dark condition were 0.099, 0.187, and 0.278 as storage temperature increased. The different values under UV treatment were 0.554, 0.588, and 0.542, as storage temperature increased from 4 to $26^{\circ}C$. As concentrations of prooxidants copper ion and cytochrome C increased, amounts of volatile components also increased. These were confirmed by DFA. Furthermore, changes in responses at each ion fragment agreed with reported results for GC/MS, which formed after rancidity of the oil, including pentane, pentanal, 1-pentanol, hexanal, n-octane, 2-hexenal, heptanal, 2-heptenal, decane, 2-octenal, undecane, and dodecane.

Environment Factors for Germination, Growing and Storage of Sprout Vegetables of Coreopsis tinctoria Nutt., Saussurea pulchella (Fisch.) Fisch. and Matricaria recutica L. (국화과 기생초, 각시취 및 저먼캐모마일 새싹채소의 발아, 재배 및 저장에 미치는 환경요인)

  • Lee, Moo-Yeul;Shin, So-Lim;Chang, Young-Deug;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.22 no.2
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    • pp.136-144
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    • 2009
  • Germination tests on 3 species that have potential for use as sprout vegetables, such as Coreopsis tinctoria, Saussurea pulchella, and Matricaria recutica, were conducted for 20 days under different temperatures of $15{\sim}30^{\circ}C$, and conditions of light and dark. C. tinctoria showed germination of 83% under $15^{\circ}C$ and dark condition after 4 days, S. pulchella 51.7% under $25^{\circ}C$ and light condition after 20 days, and M. recutica 90.3% under $25^{\circ}C$ and dark condition after 4 days. To investigate optimum plantlet size before greening treatment, seeds germinated were allowed to grow under darkness. The optimum growth of C. tinctoria was obtained under $30^{\circ}C$ after 5 days, S. pulchella under $25^{\circ}C$ after 6 days, and M. recutica under $20^{\circ}C$ after 6 days. Greening treatment resulted in diminished longitudinal growth, but C. tinctoria and S. pulchella showed more vigorous latitudinal growth. Days required before marketing as sprout vegetables were different according to species - Three days of greening was good for C. tinctoria, 2 for S. pulchella, no greening for M. recutica. Generally, tightly sealed containers for minimum water loss were recommended for storage of sprout vegetables after harvest. However, storage methods for each species were different depending on various factors like temperature of storage, presence of ventilation holes of storage packages and forms of marketing. More detailed research for above 3 species is proposed.

Surface Passivation Method for GaN UV Photodetectors Using Oxygen Annealing Treatment

  • Lee, Chang-Ju;Park, Hongsik
    • Journal of Sensor Science and Technology
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    • v.25 no.4
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    • pp.252-256
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    • 2016
  • Epitaxially grown GaN layers have a high surface state density, which typically results in a surface leakage current and a photoresponse in undesirable wavelengths in GaN optoelectronic devices. Surface passivation is, therefore, an important process necessary to prevent performance degradation of GaN UV photodetectors. In this study, we propose oxygen-enhanced thermal treatment as a simple surface passivation process without capping layers. The GaN UV photodetector fabricated using a thermal annealing process exhibits improved electrical and photoresponsive characteristics such as a reduced dark current and an enhanced photoresponsive current and UV-to-visible rejection ratio. The results of this study show that the proposed surface passivation method would be useful to enhance the reliability of GaN-based optoelectronic devices.

Causes and treatment of Melasma in Sasang constitutional medicine (사상의학(四象醫學)으로 본 여성 기미의 원인과 치료)

  • Song, Young-Hoon;Yoo, Dong-Yul
    • Journal of Haehwa Medicine
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    • v.18 no.2
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    • pp.105-111
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    • 2009
  • Melasma is a common disorder that causes dark colored patches. It generally causes brown spots on the face, especially on the forehead, cheeks, and upper lips. The pattern of patches has bilateral symmetry in yellowish-brown to gray-brown colors. It is much more common in women than in men. Melasma is considered to be caused by environmental and physical constitutional factors and often occurs when a woman's hormone changes by pregnancy or the use of oral contraceptive pills. It is important to combine oriental medicine and Sasang constitutional medicine for treatment of Melasma. Normal treatment of Melasma includes warming oneself, removal of the emotional stress factors, and good nutrition.

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The bibliographical study on the cause, symptom and treatment of Gi-Su(氣嗽) (기수(氣嗽)에 대(對)한 문헌적(文獻的) 고찰(考察))

  • Kim, Jung-Hwa
    • The Journal of Internal Korean Medicine
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    • v.13 no.1
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    • pp.80-85
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    • 1992
  • This study has been carried out to investigate the cause, symptom and treatment of Gi-Su(氣嗽) by referring to 20 literatures. The results were as follows; 1. The factors causing Gi-Su(氣嗽) divided into 3 groups. The 1st outer factors are mental element, 2nd spleen week 3rd dam(痰). 2. The symptom of Gi-Su(氣嗽) is as follows. stimulus feeling in throat, cough, pain of sub-ribs, bad feeling, retching, vomiting, unfair, fatigue, worn, diarrhea, dark tongue, makhyun(脈弦). 3. The treatment of Gi-Su(氣嗽) is as follows. gaewulhanggi(關鬱行氣), egichungpae(利膈淸肺), hwadam(化痰), soganhaeWul(疏肝解鬱), hanggidoche(行氣導滯), sangyuljedam(散結除痰) 4. The drugs of Gi-Su(氣嗽) is as follows. chilgitang(七氣湯), gamisachiltang(加味四七湯), sojaganggitang(蘇子降氣湯), banhahubaktang(半夏厚朴湯).

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Effect of Artificial Light Source on the Growth and Quality of Lettuce

  • Hyeon-Do Kim;Yeon-Ju Choi;Eun-Young Bae;Byoung-Il Je;Seung-Min Song;Jum-Soon Kang
    • Journal of Environmental Science International
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    • v.33 no.6
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    • pp.383-402
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    • 2024
  • Variations in lettuce growth and quality were observed depending on the type of artificial light source. The RGB LED treatment resulted in thick leaf development, leading to higher fresh weight, dry weight, and relative growth rates. Two cultivars, "Tomalin" and "seonpunggold," exhibited increased anthocyanin content and dark red leaf color under conditions of RGB LED treatment. Additionally, they exhibited high chlorophyll content under conditions of RGB LED and RGBFR LED treatments. Particularly, under Red LED treatment, the plants showed elongated leaves with narrow widths, resulting in a higher leaf shape index and a tendency towards leaf curling. Therefore, RGB LED lighting which appropriately blends red, blue, and green lights, is more effective than single lighr sources at improving lettuce growth and quality.

Inhibitory Effects of ABA and $Ca^{2+}$ on Dark Respiration in Protoplasts Isolated from the Basal Intercalary Meristematic Tissues of Oat Leaves (귀리잎의 기저부 절간분열조직에서 분이한 원형질체의 암호흡 활성에 미치는 ABA와 $Ca^{2+}$의 억제효과)

  • 홍영남
    • Journal of Plant Biology
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    • v.38 no.2
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    • pp.195-201
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    • 1995
  • The present study was made of the effects of abscisic acid(ABA) and calcium ions on dark respiration in protoplasts isolated from the basal intercalary meristematic tissues of oat (Avena sativa L.) seedlings. The influences of calcium channel blockers diitiazem(DTZ), verapamil(VPM), and $LaCl_2$ and the calmodulin antagonist trifluoperazine(TFP) on protoplast respiration activities were also investigated in order to evaluate the possible involvement of calcium channels and calmodulin during the dark respiration. The ABA only caused an 21% inhibition of protoplast respiration at $10^{-6}\;M$, but the extent of inhibition was very low by calcium treatments in the absence of ABA. In the presence of $10^{-6}\;M$ ABA, however, this inhibition of respiration increased by the increment of calcium ions concentrations. Treatments of DTZ and VPM were all found to restore the calcium-dependent inhibition of protoplast respiration by ABA and it was the same in thc $LaCl_2$ treatment except at $10^{-4}\;M$. At concentration from $10^{-6}\;M\;to\;10^{-4}\;M$, TFP also restored an inhibition of respiration. These results support the possibility that ABA increases plasmalemma permeability to calcium ions which might then bind to calmodulin to regulate oat protoplast dark respiration.ration.

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Analysis of Chemical Compounds on Tuna Processing By-products (참치가공 부산물의 부위별 성분 분석)

  • 강치희;정혜영;이대희;박재갑;하정욱;이승철;황용일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.6
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    • pp.981-986
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    • 2000
  • In the process of tuna for food, the unprocessed parts of the whole fish range from 30 to 35%, which are mostly used as animal feed stuff, and wasted. Thus, preliminary study on the recycling of the by-product was done to ultimately produce highly valuable products. The study was peformed on skin, skin flesh, tail flesh, dark flesh and abdominal flesh of the tuna. First, crude protein was the most abundant in the skin at 26.31%. About 21% of the crude protein were found in the other samples. Second, crude fat was greatly obtained in the skin at 15.58%. Interestingly, only 0.75% of crude fat was found in the dark flesh. Third, vitamin C and vitamin B group were highly measured in the abdominal flesh and the dark flesh, respectively. Importantly, docosahexaenoic acid (DHA, C22 : 6 $\omega$ -3) and eicosapentaenoic acid (EPA, C22 : 6 $\omega$ -3) which are highly unsaturated fatty acids, were abundant in the skin and skin flesh. To determine the effect of high temperature to the unprocessed parts, the samples were exposed to steam for 30 min. After this processing, 15-methylhexadecanoic acid (i-17 : 0) were in- creased in the skin flesh and the dark flesh. Furthermore, 3-hydroxytetradecanoic acid (3-OH 14 : 0), heptadecanoic acid (17 : 0), 2-hydroxyhexadecanoic acid (2-OH 16 : 0) and nonadecanoic acid (19 : 0) hat were not found before with steam were detected. The DHA and EPA in the dark flesh and the DHA in the skin were stable, respectively, even after the treatment.

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Growth and Bioactive Compound Contents of Various Sprouts Cultivated under Dark and Light Conditions (광 유무에 따른 다양한 새싹 채소의 생육 및 생리활성 화합물의 함량)

  • Lee, Jin-Hui;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.30 no.3
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    • pp.218-229
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    • 2021
  • Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m-2·s-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.