• Title/Summary/Keyword: Dark treatment

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Dark-chilling Pretreatment Protects PSI from Light-chilling Damage

  • Kudoh, Hideki;Sonoike, Kintake
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.59-62
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    • 2002
  • In chilling-sensitive plants, the donor side of Photosystem II is inhibited by the chilling treatment in the dark, while the acceptor side of Photosystem I is inhibited by the chilling under the moderate light. Since the addition of inhibitors of electron transfer from Photosystem II protects Photosystem I from chilling induced photoinhibition of Photosystem I, inhibition or down-regulation of Photosystem II activity in vivo may also protect Photosystem I from photoinhibition. It was revealed that dark-chilling pretreatment actually protected Photosystem I from photoinhibition. The results imply that down-regulation of Photosystem II under stress conditions may have a role to protect Photosystem I from photoinhibition.

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Effects of Light Source, Plant Growth Regulators (GA, BA, ABA) and Sodium Hypochlorite on 'Grand Rapid' Lettuce (Lactuca Sativa L.) Seed Germination (광질(光質), 생장조절물질(生長調節物質) (GA, BA, ABA) 및 Sodium Hypochlorite의 처리가 상치 (Lactuca sativa L.) 종자의 발아(發芽)에 미치는 영향(影響))

  • Lee, Young Bok;Kim, Young Rae
    • Korean Journal of Agricultural Science
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    • v.10 no.2
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    • pp.242-248
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    • 1983
  • The effects of light source, plant growth regulators (GA, BA, ABA), and sodium hypochlorite (4% Cl, SH) on germination of 'G rand Rapid' lettuce (Lactuca sativa L.) seed were studied. Seed did not germinate under the conditions of dark and blue light. The treatment of GA or BA did not promote on germination under dark or blue light. The germination percentage under red light was very high, it was similar to the condition of white light. ABA inhibited lettuce seed germination. Although ABA was treated, BA 10ppm treatment was moderately effective under white light condition and BA 10ppm or 50ppm combined with GA 50ppm were also effective on seed germination under dark condition. Presoaking in sodium hypochlorite solution induced germination of lettuce seed in any condition of dark or white light. The optimum presoaking time in sodium hypochlorite solution was twenty min.

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Parametric study of brewery wastewater effluent treatment using Chlorella vulgaris microalgae

  • Choi, Hee-Jeong
    • Environmental Engineering Research
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    • v.21 no.4
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    • pp.401-408
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    • 2016
  • The aim of this study was to evaluate the biomass and lipid production of Chlorella vulgaris and its nutrient removal capability for treatment of brewery wastewater effluent. The results indicate that the maximum biochemical oxygen demand (BOD) (91.43%) and chemical oxygen demand (COD) (83.11%) were removed by C. vulgaris with aeration in the absence of light. A maximum of 0.917 g/L of dry biomass was obtained with aeration in the dark conditions, which also demonstrated the highest amount of unsaturated fatty acids at 83.22%. However, the removal of total nitrogen (TN) and total phosphorus (TP) with these aeration and light conditions was 9.7% and 11.86% greater than that of other conditions. The removal of BOD and COD and the production of biomass and lipids with aeration in the dark and the TN and TP removal with aeration and light were more effective than other conditions in the brewery wastewater effluent in the presence of C. vulgaris.

Effects of Sulfur Dioxide on Catalase Activity in Plants (식물체의 catalase 활성에 미치는 아황산가스의 영향)

  • 성민웅
    • Journal of Plant Biology
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    • v.17 no.1
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    • pp.3-8
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    • 1974
  • The experiments were conducted to examine the effects of sulfur dioxide on catalase activity in germinating seeds under dark conditions and in green plants under light conditions. Three kinds of plants, bean (Glycine max M.), cabbage (Brassica oleracea L. Chungbang No.1) and rice (Oryza sativa L. Suwon No.213-1) were used in this study. Both thegerminating seeds and the leaves were treated with various concentrations of sulfur dioxide for both one green plants with two and twelve hours. In the results of the measurement of catalase activity it was found that plant injuries by sulfur dioxide for the period of photosynthesis were higher than those for the period of respiration. In the control, apparent catalase activity under the light condition of photosynthesis showed a considerable decrease in comparison with catalase activity under the dark condition of respiration. This tendency also appeared markedly in the gas treatment. In the gas treatment for twelve hours, the decrease of catalase activity was higher than that of one hour in both photosynthesis and respiraton. It was thought that sulfur dioxide was an inhibitor of catalase activity in higher concentration of the gas.

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Isolation and Sequence Analysis of Ycf4 Gene from Zoysia japonica Steud.

  • Kim, Yang Ji;Lee, Hyo Yeon;Hyun, Hwa Ja
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.100-100
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    • 2018
  • Zoysia japonica Steud.(Zj) is a typical warm-season Korean lawn grass, which is used in many places such as river banks, roadside and soccer fields in Korea. Recently, it has also been used in school yards and the Saemangeum reclaimed land to reduce water pollution. Although the cultivated area of turfgrass is steadily increasing worldwide, it grows fast requiring frequent mowing and is difficult to grow in shady areas and the cold region. Therefore this study aims searching for useful gene(s) to develop abiotic stress tolerant and dwarf zoysiagrass. We isolated Ycf4 gene based on the sequence from Oryza sativa Japonica through RT-PCR and RACE PCR. Ultimately, open reading frame (ORF) of ZjYcf4 was 558bp long, encoding a protein of 186 amino acid residues. NCBI blast results showed that the ZjYcf4 protein is evolutionarily closely related to Ycf4 protein from Zoysia macrantha and Setaria italica (100% and 98%, respectively). To determine whether ZjYcf4 was involved in environmental stress in wild-type zoysiagrass, expression patterns of the gene were analyzed by real-time PCR under salt, cold and dark conditions. They were analyzed after each stress treatment for 3 hours. In salt and cold stresses, the expression was higher compared to control (3-fold and 1.5-fold, respectively), although there was a 1.6-fold decrease in expression under dark stress treatment. As reported previously, we suggest that ZjYcf4 gene affects abiotic stress such as salt, cold and dark.

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Effect of Seed Treatment on Improved Germinability of Agastache rugosa O. Kuntze (배초향 종자처리가 발아성 향상에 미치는 영향)

  • Joo, Hyeong-Gyu;Lee, Jung-Eun;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.30 no.1
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    • pp.77-85
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    • 2021
  • This study was carried out to establish optimal conditions for breaking dormancy of Agastache rugosa O. Kuntze seeds. A series of experiments according to seed maturity and treatment with plant growth regulators were performed to improve germination percentage and synchronize germination of the seeds. In addition, it was conducted to test whether the useful effect of seed treatment before sowing leads to healthy seedling and early vigorous growth. The average seed size was 1.85 mm (length) × 0.82 mm(width). The seed size was much smaller than other vegetable seeds. Seeds colorappeared dark brown, the shape of the seeds was oval and the weight of 1,000seeds was 352.8 mg. The optimum germination temperature was 22℃. Light exposure during germination did not affect germination promotion, suggesting that A. rugosa seeds are a kind of dark germinating seeds. Seed dormancy lasted for 40 days after harvesting, and GA3 treatment of dormant seeds could break dormancy. There were significant differences in germination percentage and rate according to the maturity of seeds. The germination percentage of mature seeds was 10 - 18% higher than that of immature seeds, and germination rate was 2 days faster. GA3 treatment during growth regulator treatment improved germinability, but BAP or ethephone treatment did not. The optimal growth regulator concentration of for germination was the combination treatment of 100 mM GA3 + 100 mM BAP.

The Role of $Ca^{2+}$ in Retardation Effects of Benzyladenine on the Senescence of Wheat (Triticum aestivum L.) Leaves

  • Hong, Kee-Jong;Jin, Chang-Duck;Hong, Young-Nam
    • Journal of Plant Biology
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    • v.39 no.2
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    • pp.113-121
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    • 1996
  • The role of Ca2+ on benzyladenine (BA)-induced senescence retardation in mature wheat (Triticum aestivum L.) primary leaves was investigated. When an extracellular calcium chelator, ethylene glycol-bis-($\beta$-aminoethylether)-N, N'-tetraacetic acid (EGTA) together with BA, was applied to senescing leaves for 4 days of dark incubation, the content of chlorophyll and soluble protein decreased rapidly. And, the content of malondialdehyde (MDA), known to be a degradation product of membrane lipids, increased compared with the BA alone control. The BA-EGTA combination also caused the stimulation of protease and RNase activity and a rapid loss of catalase activity owing to the decling of BA effects. In the case of treatment with only intracellular calcium antagonist 3, 4, 5-trimethoxybenzoic acid 8-(diethylamino) octyl ester (TMB-8) without the BA addition, the chlorophyll content at day 4 after dark incubation decreased in paralled with the increasing concentration of the antagonist. In addition, the chlorophyll content at 10-5 M calcium ionophore A23187 treatment in the absence of BA was similar to that of the BA alone treatment. These results suggest that calcium may mediate the retardation effect of BA on leaf senescence by acting as a second messenger and that the calcium input from cell organelles, as well as the calcium inflow from intercellular spaces and cell walls, may be involved in modulating cytosolic calcium levels related to BA action.

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Light Quality during Seed Imbibition Affects Germination and Sprout Growth of Soybean

  • Kang, Jin-Ho;Park, A-Jung;Jeon, Byung-Sam;Yoon, Soo-Young;Lee, Sang-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.292-296
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    • 2002
  • Soybean seeds were treated with blue, red and far-red lights for 0, 6, 12, and 24 hours during 24-hour imbibition before culture for 6 days. The soybean sprouts raised were classified by their hypocotyl lengths; normal (>4cm), abnormal (<4cm) and non-germination, and their lateral roots, hypocotyl diameters and component dry weights were measured. Red light treatment and dark imbibition reduced the abnormal soybean sprouts more than far-red and blue light treatments, meaning that the former treatments produced more commercial sprouts. The lateral roots were more formed in blue light and dark imbibition than the other light treatments, but were completely blocked by any light treatment lasted during the whole imbibition. Although any light quality treatment did not influence their primary root lengths, blue light one lengthened the hypocotyl more than the others treated during the imbibition, and far-red light enlarged its diameter. Despite this morphological change, component, total or economic yield was not significantly different among the light quality treatments during the imbibition.

Effect of Varying Lighting Regimes on Broiler Performance (Broiler생산에 있어 점등방법이 그 생산능력에 미치는 영향)

  • 유창우;오세정
    • Korean Journal of Poultry Science
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    • v.14 no.1
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    • pp.55-61
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    • 1987
  • Four treatments were conducted to determine the effects of lighting regimes on the body weight gain and feed conversion of broiler chicks during 7 weeks: 1) 24 hours consistant lighting regime, 2) intermittent lighting regime of 1 hour lighting and 3 hours dark, 3) 20 hours lighting and 4 hours dark regime, 4) natural lighting regime. Each treatment was composed of three replications and 180 broiler male chicks were used in this experiment (45 chicks for each treatment,15 chicks for each replication). The results of this experiment were summarized as follows; 1. The body weight gains of 1 hour lighting+3 hours dark regime were heavier than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the body weight gains of 1 hour lighting+3 hours dark regime and 20 hours lighting+4 hours dark regime were much heavier than those of natural lighting regime and 24 hours consistant lighting regime, but in 5-7 weeks, no differences were found among the 4 treatments. 2. The feed conversions of 1 hour lighting+3 hours dark regime were improved more than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the feed conversions of 1 hour lighting+3 hours dark regime and 20 hours lighting+4 hours dark regime were much more improved than those of natural lighting regime and 24 hours consistant lightine regime, but in 5-7 weeks, no differences were found among the 4 treatments. 3. These results indicated that the intermittent lighting regimes such as 1 hour lighting+3 hours dark and 20 hours lighting+4 hours dark are more efficient on the body weight gain and feed conversion of broiler chicks than natural lighting regime and 24 hours consistant lighting regime.

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Properties of Bentwood for Esthetic Designing by Microwave and Ammonia Solution Treatment

  • Lee, Won-Hee;Bae, Hyun-Mi
    • Journal of the Korea Furniture Society
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    • v.18 no.4
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    • pp.275-281
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    • 2007
  • This study was carried out to know bending process property of bentwood by treatment methods such as microwave and ammonia solution treatment. Bentwood processing was operated on bending-jig form with 60mm and 100mm radius of curvature (ROC). The species was used to Korean red pine (Pinus densiflora). No significant difference was shown in bentwood processing method on ROC. In treatment method, ammonia solution treatment for bentwood was better in dimensional stability and working ability than that of microwave treatment. However, bentwood stress was reduced with ammonia solution treatment. Color of bentwood surface appearance has a dark tendency in ammonia solution treatment. But it seems to beautiful effect of apparent annual ring is rising esthetically by ammonia solution treatment. To utilize wood as a exterior material for living amenity, it has to be closely examined from various angles including surface property, degree of swelling and shrinkage, drying state, retaining strength, durability, safety, and insect damage.

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