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External and Internal Glucose Mass Transfers in Succinic Acid Fermentation with Stirred Bed of Immobilized Actinobacillus succinogenes under Substrate and Product Inhibitions

  • Galaction, Anca-Irina;Rotaru, Roxana;Kloetzer, Lenuta;Vlysidis, Anestis;Webb, Colin;Turnea, Marius;Cascaval, Dan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1257-1263
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    • 2011
  • This paper is dedicated to the study on the external and internal mass transfers of glucose for succinic acid fermentation under substrate and product inhibitions using a bioreactor with stirred bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the kinetic model adapted for both inhibitory effects, specific mathematical models were developed for describing the profiles of the substrate concentration in the outer and inner regions of biocatalysts and for estimating the substrate mass flows in the liquid boundary layer surrounding the particle and inside the particle. The values of the mass flows were significantly influenced by the internal diffusion velocity and rate of the biochemical reaction of substrate consumption. These cumulated influences led to the appearance of a biological inactive region near the particle center, its magnitude varying from 0 to 5.3% of the overall volume of particles.

Particle image velocimetry measurement of complex flow structures in the diffuser and spherical casing of a reactor coolant pump

  • Zhang, Yongchao;Yang, Minguan;Ni, Dan;Zhang, Ning;Gao, Bo
    • Nuclear Engineering and Technology
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    • v.50 no.3
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    • pp.368-378
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    • 2018
  • Understanding of turbulent flow in the reactor coolant pump (RCP) is a premise of the optimal design of the RCP. Flow structures in the RCP, in view of the specially devised spherical casing, are more complicated than those associated with conventional pumps. Hitherto, knowledge of the flow characteristics of the RCP has been far from sufficient. Research into the nonintrusive measurement of the internal flow of the RCP has rarely been reported. In the present study, flow measurement using particle image velocimetry is implemented to reveal flow features of the RCP model. Velocity and vorticity distributions in the diffuser and spherical casing are obtained. The results illuminate the complexity of the flows in the RCP. Near the lower end of the discharge nozzle, three-dimensional swirling flows and flow separation are evident. In the diffuser, the imparity of the velocity profile with respect to different axial cross sections is verified, and the velocity increases gradually from the shroud to the hub. In the casing, velocity distribution is nonuniform over the circumferential direction. Vortices shed consistently from the diffuser blade trailing edge. The experimental results lend sound support for the optimal design of the RCP and provide validation of relevant numerical algorithms.

Optimized Operation of Dual-Active-Bridge DC-DC Converters in the Soft-Switching Area with Triple-Phase-Shift Control at Light Loads

  • Jiang, Li;Sun, Yao;Su, Mei;Wang, Hui;Dan, Hanbing
    • Journal of Power Electronics
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    • v.18 no.1
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    • pp.45-55
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    • 2018
  • It is usually difficult for dual-active-bridge (DAB) dc-dc converters to operate efficiently at light loads. This paper presents an in-depth analysis of a DAB with triple-phase-shift (TPS) control under the light load condition to overcome this problem. A kind of operating mode which is suitable for light load operation is analyzed in this paper. First, an analysis of the zero-voltage-switching (ZVS) constraints for the DAB converter has been carried out and a reasonable dead-band setting method has been proposed. Secondly, the basic operating characteristics of the converter are analyzed. Third, under the condition of satisfying the ZVS constraints, both the reactive power and the root mean square (RMS) value of the current are simultaneously minimized and a particle swarm optimization (PSO) algorithm is employed to analyze and solve this optimization problem. Lastly, both simulations and experiments are carried out to verify the effectiveness of the proposed method. The experimental results show that the converter can effectively achieve ZVS and improved efficiency.

Numerical Research on Suppression of Thermally Induced Wavefront Distortion of Solid-state Laser Based on Neural Network

  • Liu, Hang;He, Ping;Wang, Juntao;Wang, Dan;Shang, Jianli
    • Current Optics and Photonics
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    • v.6 no.5
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    • pp.479-488
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    • 2022
  • To account for the internal thermal effects of solid-state lasers, a method using a back propagation (BP) neural network integrated with a particle swarm optimization (PSO) algorithm is developed, which is a new wavefront distortion correction technique. In particular, by using a slab laser model, a series of fiber pumped sources are employed to form a controlled array to pump the gain medium, allowing the internal temperature field of the gain medium to be designed by altering the power of each pump source. Furthermore, the BP artificial neural network is employed to construct a nonlinear mapping relationship between the power matrix of the pump array and the thermally induced wavefront aberration. Lastly, the suppression of thermally induced wavefront distortion can be achieved by changing the power matrix of the pump array and obtaining the optimal pump light intensity distribution combined using the PSO algorithm. The minimal beam quality β can be obtained by optimally distributing the pumping light. Compared with the method of designing uniform pumping light into the gain medium, the theoretically computed single pass beam quality β value is optimized from 5.34 to 1.28. In this numerical analysis, experiments are conducted to validate the relationship between the thermally generated wavefront and certain pumping light distributions.

Development Study of Mono-Propellant Micro Propulsion Using MEMS Technology

  • Dan, Yoichiro;Kishida, Masahiro;Ikuta, Tatsuya;Takahashi, Koji
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2004.03a
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    • pp.597-600
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    • 2004
  • Fabrication technique and performance test of catalytic micro propulsion are treated based on MEMS technology. This propulsion is designed to use hydrogen peroxide as liquid mono-propellant for attitude control of pica-satellite. The propellant is fed into the micro reactor channel and decomposed into hot gas yielding controllable thrust by catalyst. In order to increase the efficiency of the reaction that depends on the contact area of propellant and catalyst, porous surface formation on the channel accompanied by platinum particle deposition has been performed using H$_2$PtCl$_{6}$ solution as a precursor. Several thrusters were fabricated in different concentration of H$_2$PtCl$_{6}$ solution to determine the best quantity of Pt particles. For the comparison of the performance of each thruster, the volume of oxygen generated by the decomposition of hydrogen peroxide and the thrust were measured.red.

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Study on the Changes of Dentinal Hypersensitivity and Surface Characteristics Following the Various Root Treatment (수종의 치근면 처치 방법에 따른 상아질 지각 과민 변화 및 표면 특성에 관한 연구)

  • Kwon, Soon-Young;Lim, Sung-Bin;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.29 no.1
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    • pp.51-63
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    • 1999
  • Exposure of the root surface due to gingival recession after periodontal surgery, elicit pain response when exposed to mechanical, heat, chemical or osmotic irritation. Especially patients treated with periodontal surgery, show high frequency. There have been reports that the 1 out of 7 patients complains of dentinal hypersensitivity. There have been many studies on the clinical effects of various materials on the treatment of dentinal hypersensitivity. The purposes of this study were to evaluate the effect of sodium chloride and potassium oxalate and to observe the relationship between the dentinal hypersensitivity and surface characteristics such as dentinal tubule size and number. This study included 20 teeth which were scheduled for extraction and had no pulpal disease. These teeth were divided into Root planing group, EDTA group, NaCl group and Oxalate group. Dentinal hypersensitivity is measured by tactile, pressured air and cold water using NRS (Numerical Rating Scales). Teeth were extracted under local anesthesia and each specimen was sectioned to a size about 3 X 5 mm and was examined under the scanning electron microscope (X2,000) The results were as follows, 1. The EDTA group exhibited significantly increased dentinal hypersensitivity comparing with the other groups. 2. The NaCl and Oxalate groups showed significantly reduced dentinal hypersensitivity comparing with the EDTA group. 3. As a method for dentinal hypersensitivity measurement, it was presumed thet tactile sensitivity test was not sensitive method but air blast test and cold water test were adequate method. 4. In a SEM study, the root planing group exhibited amorphous smear layer and showed no dentinal tubule orifice, but the EDTA group showed the large number of dentinal tubules. On the other hand, the NaCl and Oxalate groups did not show exposed dentinal tubules. The NaCl group showed more rough root surface than the EDTA group, and the Oxalate group showed many participates to be presumed as calcium oxalate particle. As the results from this study, root planing couldn't expose the dentinal tubule and NaCl and potassium oxalate occluded exposed dentinal tubule effectively. Dentinal hypersensitivity has close relationship with the exposure of dentinal tubules, especially with it's size and number.

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Characterization of a Cell Line HFH-T2, Producing Viral Particles, from Primary Human Fetal Hepatocytes Infected with Hepatitis B Virus

  • Shim, Jae-Kyoung;Kim, Dong-Wook;Chung, Tae-Ho;Kim, June-Ki;Suh, Jeong-Ill;Park, Chun;Lee, Young-Choon;Chung, Tae-Wha;Song, Eun-Young;Kim, Cheorl-Ho
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.186-192
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    • 2001
  • A primary culture of human fetal hepatocytes was obtained through a therapeutic abortion process at 26 weeks of gestation period. More than $10^8$ cells were seeded on a plastic plate. These hepatocytes were infected with hepatitis B virus (HBV). The HBV was purified from serum of one chronic HBV carrier. Transformed hepatocytes were subcultured in a 10% FBS-supplemented medium. The morphology of the transformed cell was epithelial-like. The cells from the first pass showed signs of early proliferation and had a latent period of more than 3 months after 6-7 passages. After the rest period, the transformed cell proliferated actively and they were subcultured every three days. Transformed hepatocytes were characterized by detection of the HBV transcript by RT-PCR. The secretion of virions from transformed cells was investigated by PCR with the cell medium. Two types of virions secreted into the culture medium were examined by using the transmission electron microscope. Another approach to study the secretion of virions in to culture medium was carried out with HBV antibody. HBsAg was detected in the culture medium of transformed cells using ELISA and Western blot analyses. These data suggested that the human fetal hepatocyte cell line has been established by infection of HBV, in which this cell line secreted viral particles into the culture medium.

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Studies on Preparation of $TiO_2$Powder with Purity and Fine Particle -A Study of High Purifying(I)- (고순도.미립 $TiO_2$분말 제조에 관한 연구 -고순도화 연구(I)-)

  • Lee, Mi-Jai;Jee, Mi-Jung;Kim, Hwan;Lee, Chul-Tae;Choi, Byung-Hyun
    • Journal of the Korean Ceramic Society
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    • v.37 no.10
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    • pp.933-937
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    • 2000
  • 고기능성 전자재료용 TiO$_2$분말 및 박막제조에 사용되는 중간생성물인 TiCl$_4$는 99% 이상의 고순도가 요구된다. 고순도.미립의 TiO$_2$분말 및 TiCl$_4$는 황산법으로 제조한 저순도 TiO$_2$원료를 사용하여 염소화법으로 Ti-염화물 및 염화불순물로 제조한 후, 대부분의 염화불순물들은 3단계 과정을 거쳐 고순화 하였다. 대부분의 염화불순물은 분리.응축 및 분별증류로, VOCl$_3$는 mineral oil을 첨가하여 비등점을 변화시켜, 그리고 미량의 염화불순물은 열가수분해하여 침전시킨 후 유기용제 처리하여 제거하였다. 유기용제 처리는 TiO$_2$분말의 고순도화에 도움이 되었으며, 입자간의 응집을 적게 하여 TiO$_2$입자크기도 작아졌다. 또한 anatase에서 rutile 결정구조로의 전이온도도 낮아지는 부수적인 효과를 보였다.

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A Range-Based Monte Carlo Box Algorithm for Mobile Nodes Localization in WSNs

  • Li, Dan;Wen, Xianbin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.8
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    • pp.3889-3903
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    • 2017
  • Fast and accurate localization of randomly deployed nodes is required by many applications in wireless sensor networks (WSNs). However, mobile nodes localization in WSNs is more difficult than static nodes localization since the nodes mobility brings more data. In this paper, we propose a Range-based Monte Carlo Box (RMCB) algorithm, which builds upon the Monte Carlo Localization Boxed (MCB) algorithm to improve the localization accuracy. This algorithm utilizes Received Signal Strength Indication (RSSI) ranging technique to build a sample box and adds a preset error coefficient in sampling and filtering phase to increase the success rate of sampling and accuracy of valid samples. Moreover, simplified Particle Swarm Optimization (sPSO) algorithm is introduced to generate new samples and avoid constantly repeated sampling and filtering process. Simulation results denote that our proposed RMCB algorithm can reduce the location error by 24%, 14% and 14% on average compared to MCB, Range-based Monte Carlo Localization (RMCL) and RSSI Motion Prediction MCB (RMMCB) algorithm respectively and are suitable for high precision required positioning scenes.

Joint Relay Selection and Resource Allocation for Delay-Sensitive Traffic in Multi-Hop Relay Networks

  • Sha, Yan;Hu, Jufeng;Hao, Shuang;Wang, Dan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.16 no.9
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    • pp.3008-3028
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    • 2022
  • In this paper, we investigate traffic scheduling for a delay-sensitive multi-hop relay network, and aim to minimize the priority-based end-to-end delay of different data packet via joint relay selection, subcarrier assignment, and power allocation. We first derive the priority-based end-to-end delay based on queueing theory, and then propose a two-step method to decompose the original optimization problem into two sub-problems. For the joint subcarrier assignment and power control problem, we utilize an efficient particle swarm optimization method to solve it. For the relay selection problem, we prove its convexity and use the standard Lagrange method to deal with it. The joint relay selection, subcarriers assignment and transmission power allocation problem for each hop can also be solved by an exhaustive search over a finite set defined by the relay sensor set and available subcarrier set. Simulation results show that both the proposed routing scheme and the resource allocation scheme can reduce the average end-to-end delay.