• Journal of Nutrition and Health
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• 제44권5호
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• pp.366-377
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• 2011

Glutathione S-transferase (GST) is a multigene family of phase II detoxifying enzymes that metabolize a wide range of exogenous and endogenous electrophilic compounds. GSTM1 and GSTT1 gene polymorphisms may account for inter-individual variability in coping with oxidative stress. We investigated the relationships between the level of lymphocyte DNA and antioxidative parameters and the effect on GST genotypes. GSTM1 and GSTT1 were characterized in 301 young healthy Korean adults and compared with oxidative stress parameters such as the level of lymphocyte DNA, plasma antioxidant vitamins, and erythrocyte antioxidant enzymes in smokers and non smokers. GST genotype, degree of DNA damage in lymphocytes, erythrocyte activities of superoxide dismutase, catalase, and glutathione peroxidase (GSH-Px), and plasma concentrations of total radical-trapping antioxidant potential (TRAP), vitamin C, ${\alpha}$- and ${\gamma}$-tocopherol, ${\alpha}$- and ${\beta}$-carotene, and cryptoxanthin were analyzed. Lymphocyte DNA damage assessed by the comet assay was higher in smokers than that in non-smokers, but the levels of plasma vitamin C, ${\beta}$-carotene, TRAP, erythrocyte catalase, and GSH-Px were lower than those of non-smokers (p < 0.05). Lymphocyte DNA damage was higher in subjects with the GSTM1- or GSTT1-present genotype than those with the GSTM1-present or GSTT1- genotype. No difference in erythrocyte antioxidant enzyme activities, plasma TRAP, or vitamin levels was observed in subjects with the GSTM1 or GSTT1 genotypes, except ${\beta}$-carotene. Significant negative correlations were observed between lymphocyte DNA damage and plasma levels of TRAP and erythrocyte activities of catalase and GSH-Px after adjusting for smoking pack-years. Negative correlations were observed between plasma vitamin C and lymphocyte DNA damage only in individuals with the GSTM1-present or GSTT1- genotype. The interesting finding was the significant positive correlations between lymphocyte DNA damage and plasma levels of ${\alpha}$-carotene, ${\beta}$-carotene, and cryptoxanthin. In conclusion, the GSTM1- and GSTT1-present genotypes as well as smoking aggravated antioxidant status through lymphocyte DNA damage. This finding confirms that GST polymorphisms could be important determinants of antioxidant status in young smoking and non-smoking adults. Consequently, the protective effect of supplemental antioxidants on DNA damage in individuals carrying the GSTM1- or GSTT1-present genotypes might show significantly higher values than expected.

• 한국응용곤충학회지
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• 제39권4호
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• pp.259-266
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• 2000

This study was carried out to investigate the status of spot damage by fruit piercing pests and the kinds of these pests on yuzu (Citrus junos) fruit in Koheung, the most chief producing district of yuzu fruit in Korea, from ’97 to ’99. The extent of spot damage by fruit piercing pests on yuzu was increasing in recent years. This damage of fruits was severe in the lower canopy than the high one from ground and intercropping groves between yuzu trees had a greater damage to compare with single cropping of yuzu. Spot damage of yuzu fruit was occurred mainly from late September to early November when yuzu fruit is enlarging and coloring yellow. The blackish concave spot on yuzu fruit surface was appeared in 3 days after introduction of Riptortus clavatus into a netted cage containing one yuzu fruit and the diameters of this spot was 8.3 mm. At 10 days after introduction, this spot changed into milky-white with 9.2 mm diameters. One concave spot has contained oil cells by 17.7 and its external appearances has unchanged until harvest. The kinds of piercing pests of yuzu fruit were investigated with 3 orders, 16 families and 37 species. These pests were classified by 11 species of bugs, 5 species of hoppers and 21 species of moths. Among them, dominant species were Physopelta gutta, Halyamorpha halys, Empoasca vitis, Aedia leucomelas, Agrotis tokionis, etc. Macroglossum bombylans and Acherontia s쇼x are newly confirmed species as the fruit piercing moths in Korea.

• 한국환경성돌연변이발암원학회지
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• 제22권2호
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• pp.83-89
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• 2002

The single cell gel electrophoresis (comet) assay is one of the useful tools for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. This study was undertaken to evaluate the status of DNA damage in peripheral lymphocytes depending on their sex, age, smoking habits, and other factors in normal healthy Korean population. The 99 volunteers included in the study and out of these, 36 volunteers were smoker and 63 volunteers were non-smoker aged between 20-59 years. All individual answered a questionnaire that assessed their general information including smoking habits and the extent of the environmental tobacco smoke (ETS) exposure, and blood samples were obtained. There was a statistically significant difference in the extent of DNA damage between smoker and non-smoker (p<0.001). A significant difference was also observed between male and female (p<0.001) and amongst the different group of age (p<0.005), however, correlation analysis showed that only smoking habit was a significant factor for DNA damage. No significant effect of smoking duration, number of cigarettes smoking a day, SPY (smoke pack years) in smokers and environmental tobacco smoke exposure in non-smokers on the status of DNA damage was observed.

• 한국중재학회지:중재연구
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• 제32권3호
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• pp.93-109
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• 2022

Cosmetics are products that consumers use every day to maintain or improve the health of their skin and hair. Therefore, the expansion of the cosmetics market leads to the expansion of disputes over cosmetic damage. Along with constant social changes, new conflicts continue to arise. In order to resolve these disputes, various consumer dispute resolution organizations and methods are required. Therefore, Alternative Dispute Resolution (ADR), an alternative method that can provide a reasonable judgment on problems that occur during the manufacture and distribution of cosmetics with expert knowledge of the industry, is required. Korea resolves disputes between consumers and manufacturers caused by cosmetics through the ADR of the Korea Cosmetics Association and the Korea Consumer Agency. It handles disputes related to accidents caused by cosmetics, offers consultation on consumer complaints on cosmetics and provides information on accidents and safety related to cosmetics. It is not possible to completely eradicate disputes from cosmetic damages. Therefore, it is necessary to expand and efficiently operate the cosmetic ADR system for consumers. In this study, the current status of cosmetic damage disputes and damage relief and the role of the domestic ADR system were reviewed. Consumers should be easily relieved from damage caused by cosmetics. By accumulating important precedents with an efficient cosmetic damage dispute resolution system, disputes over cosmetic damage should be smoothly resolved.

• Journal of Nutrition and Health
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• 제39권1호
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• pp.18-27
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• 2006

Smoking is well known to be associated with increased indices of tree radical-mediated damage of DNA, indicating that smoking may exacerbate the initiation and propagation of oxidative stresses, which are potential underlying processes in the pathogenesis of many diseases. The purpose of this study was to evaluate whether a daily regimen of green vegetable drink supplementation to smokers can be protective against endogenous lymphocytic DNA damage and whether it could enhance other antioxidant status. Twenty nonsmokers and nineteen smokers aged 23-60 were given 240 ml of green vegetable drink every day for 8 weeks in addition to their normal diet, and blood samples were drawn before and after the intervention. The 8 weeks of green vegetable drink consumption resulted in a significant decrease (p = 0.000, by paired t-test) in lymphocyte DNA damage expressed by TL (before: $63.13{\pm}1.05$ vs after: $37.86{\pm}10.83$, before: $66.73{\pm}1.24$ vs after: $36.51{\pm}1.13$), TM (before: $14.55{\pm}0.61$ vs after: $6.61{\pm}0.25$, before: $15.36{\pm}0.45$ vs after: $6.65{\pm}0.38$) and $\%$ DNA in tail (before: $19.7{\pm}0.41$ vs after: $16.6{\pm}0.37$, before: $20.6{\pm}0.31$ vs after: $17.1{\pm}0.5$) in both nonsmokers and smokers respectively. Vitamin C and TRAP level was not significantly changed after the supplementation. In conclusion, these results support the hypothesis that green vegetable drink exert a cancer-protective effect partially via a decrease in oxidative damage to DNA.

• 콘크리트학회논문집
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• 제14권6호
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• pp.805-812
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• 2002

건설된지 오래되어 보강이 필요한 구조물이 증가하고 있다. 이런 구조물들은 현재 상태에 대한 정확한 평가가 있어야만 보강여부 및 보강 방법 등을 결정할 수 있다. 따라서 손상정도를 평가하는 객관적인 방법이 절실히 필요하나, 균열이나 처짐과 같이 관측이 용이한 구조물의 변화 특성을 이용하여 기존 구조물의 손상 상태를 평가하는 연구가 지금까지는 거의 없는 상태이다. 본 연구에서는 하중의 증가에 따른 균열 특성을 측정, 분석하고, 균열 특성 값과 실 구조물의 손상상태, 또는 재하 상태와의 상관관계를 규명하기 위하여, 11개의 실험체를 대상으로 다양한 균열 특성 값을 측정, 도출, 분석하였다. 본 연구에서 검토된 균열 특성 값에는 균열 개수, 균열 길이, 균열 범위, 균열 간격, 최대 균열길이, 균열 면적, 평균 균열길이 등이 검토되었다. 분석결과는 항복 하중시 측정된 처짐 값을 기준으로 각각 하중 단계별 처짐 값을 백분율로 명시한 상대 처짐 값과 표준화된 균열 특성 값의 상관관계를 규명하였다. 이렇게 표준화된 여러 개의 균열 특성 값 중 균열 간격, 균열 면적, 균열 범위, 최대 균열길이 등의 상대 처짐 값과의 상관관계가 밀접한 것으로 나타났다. 따라서, 실 구조물에서도 균열을 측정하여 표준값을 찾아내면, 그 구조물에 작용하는 하중의 크기나 과거에 받았던 최대 하중의 크기를 추정하는데 사용될 수 있을 것으로 기대된다. 구조물의 손상단계를 보다 신뢰성 높게 추정하기 위해서는 실험체의 제원, 콘크리트 및 철근의 강도, 철근비 등의 다양한 변수가 앞으로도 계속 연구되어야 할 것이다.

• 전기전자학회논문지
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• 제25권4호
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• pp.750-755
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• 2021

본 논문에서는 YOLOv4를 이용하여 차량의 부위별 파손현황을 검출하는 기법을 제안한다. 제안 알고리즘은 YOLOv4를 통해 차량의 부위와 파손을 각각 학습시킨 후 검출되는 바운딩 박스의 좌표 정보들을 추출하여 파손과 차량부위의 포함관계를 판단하는 알고리즘을 적용시켜 부위별 파손현황을 도출한다. 또한 성능비교의 객관성을 위하여 동일분야의 VGGNet을 이용한 기법, 이미지 분할과 U-Net 모델을 이용한 기법, Weproove.AI 딥러닝 모델 등을 대조 모델로 포함한다. 이를 통하여 제안 알고리즘의 성능을 비교, 평가하고 검출 모델의 개선 방안을 제안한다.

• 한국공간구조학회논문집
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• 제18권2호
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• pp.87-97
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• 2018

Stone pagoda structures have continued to be aged due to the combination of various damage factors. However, some studies on nonstructural damage have been carried out, but assessment studies on structural damage have not been done in various ways. Therefore, in this study, structural and nonstructural influencing factors according to the damage types are classified and the damage assessment according to the structural influencing factors affecting the behavior of the stone pagoda structure is performed. In addition, the damage rating classification criteria for each type of structural damages or damage locations are presented, and the damage index is calculated by providing the criteria for the classification of damage according to the degree of damage to which the damage is caused. Therefore, this study can evaluate quantitatively the damage status of stone pagoda structures.

• Archives of Pharmacal Research
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• 제29권7호
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• pp.577-581
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• 2006

Oxidative mechanisms are thought to have a major role in cataract formation and diabetic complications. Antioxidant enzymes play an essential role in the antioxidant system of the cells that work to maintain low steady-state concentrations of the reactive oxygen species. When HLE-B3 cells, a human lens cell line were exposed to 50-100 mM glucose for 3 days, decrease of viability, inactivation of antioxidant enzymes, and modulation of cellular redox status were observed. Significant increase of cellular oxidative damage reflected by lipid peroxidation and DNA damage were also found. The glycation-mediated inactivation of antioxidant enzymes may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition and may contribute to various pathologies associated with the long term complications of diabetes.

• 기술사
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• 제43권1호
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• pp.46-49
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• 2010

Sea temperature rise by global warming, depletion of fishery resources and ocean pollution have caused unusual population explosion of the jellyfish, The blooming of jellyfish have brought social problem and economic damage, Therefore the interdisciplinary study of jellyfish by scientists and countermeasure are needed.