Browse > Article

The Effect of Green Vegetable Drink Supplementation on Cellular DNA Damage and Antioxidant Status of Korean Smokers  

Kim Hye-Young (Department of Food and Nutrition, Hannam University)
Park Yoo Kyoung (Department of Medical Nutrition, College of East-West Medical Science, Kyung Hee University)
Kim Tae Seok (R&D, Pulmuone Co., Ltd.)
Kang Myung-Hee (Department of Food and Nutrition, Hannam University)
Publication Information
Journal of Nutrition and Health / v.39, no.1, 2006 , pp. 18-27 More about this Journal
Abstract
Smoking is well known to be associated with increased indices of tree radical-mediated damage of DNA, indicating that smoking may exacerbate the initiation and propagation of oxidative stresses, which are potential underlying processes in the pathogenesis of many diseases. The purpose of this study was to evaluate whether a daily regimen of green vegetable drink supplementation to smokers can be protective against endogenous lymphocytic DNA damage and whether it could enhance other antioxidant status. Twenty nonsmokers and nineteen smokers aged 23-60 were given 240 ml of green vegetable drink every day for 8 weeks in addition to their normal diet, and blood samples were drawn before and after the intervention. The 8 weeks of green vegetable drink consumption resulted in a significant decrease (p = 0.000, by paired t-test) in lymphocyte DNA damage expressed by TL (before: $63.13{\pm}1.05$ vs after: $37.86{\pm}10.83$, before: $66.73{\pm}1.24$ vs after: $36.51{\pm}1.13$), TM (before: $14.55{\pm}0.61$ vs after: $6.61{\pm}0.25$, before: $15.36{\pm}0.45$ vs after: $6.65{\pm}0.38$) and $\%$ DNA in tail (before: $19.7{\pm}0.41$ vs after: $16.6{\pm}0.37$, before: $20.6{\pm}0.31$ vs after: $17.1{\pm}0.5$) in both nonsmokers and smokers respectively. Vitamin C and TRAP level was not significantly changed after the supplementation. In conclusion, these results support the hypothesis that green vegetable drink exert a cancer-protective effect partially via a decrease in oxidative damage to DNA.
Keywords
green vegetable drink; supplementation; lymphocyte; DNA damage; antioxidant status; vitamin C; TRAP; smokers;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Pool-Zobel BL, Bub A, Muller H, Wollowski I, Rechkemmer G. Consumption of vegetables reduces genetic damage in humans: First results of a human intervention trial with carotenoid-rich Foods. Carcinogenesis 18(9): 1847-1850, 1997   DOI   ScienceOn
2 Collins BH, Horska A, Hotten PM, Riddoch C, Collins AR. Kiwifruit protects against oxidative DNA damage in human cells and in vitro. Nutr Cancer 39(1): 148-153, 2001   DOI   ScienceOn
3 Park JC, Cho YS, Park SK, Park JR, Jeon SS, Ok KD, Choi JW. Isolation of flavone-7-O-glycosides from the aerial parts of Angelica Keiskei and anti-hyperlipidemic effect. Korean J Parmacogn 26(4): 337-373, 1995   과학기술학회마을
4 Kim JS, Kim HY, Park YK, Kim TS, Kang MH. The effects of green vegetable juice (Angelica Keiskei) supplementation on plasma lipids and antioxidant status in smokers. Korean J Nutr 36(9): 933-941, 2003   과학기술학회마을
5 Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayshi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel Comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis 35: 206-221, 2000   DOI   ScienceOn
6 Dietrich M, Block G, Norkus EP, Hudes M, Traber MG, Cross CE, Packer L. Smoking and exposure to environmental tobaco smoke decrease some plasma antioxidants and increase gammatocopherol in vivo after adjustment for dietary antioxidant intakes. Am J Clin Nutr 77(1): 160-166, 2003   DOI
7 Park JA, Kang MH. Vitamin C intakes and serum levels in smoking college students. Korean J Nutr 29(1): 122-133, 1996   과학기술학회마을
8 Riso P, Pinder A, Santangelo A, Porrini M. Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage? Am J Clin Nutr 69: 712-712, 1999   DOI
9 Genser D, Kang M-H, Vogelsang H, Elmadfa I. Status of lipidsoluble antioxidants and TRAP in patients with crohn's disease and healthy controls. Eur J Clin Nutr 53: 675-679, 1999   DOI
10 Hertog MG, Kromhout D, Aravanis C, Blackburn H, Buzina R, Fidanza F, Giampaoli S, Jansern A, Menotti A, Nedeljkovic S, Pekkarinen M, Simic BS, Toshima H, Feskens EJ, Hollman PC, Katan MB. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch Intern Med 155: 381-386, 1995   DOI
11 Porrini M, Riso P, Oriani G. Spinach and tomato consumption increases lymphocyte DNA resistance to oxidative stress but this is not related to cell carotenoid concentrations. Eur J Nutr 41: 95-100, 2002   DOI   ScienceOn
12 Mary N, Mohankumar S, Janani B, Karthikeya Prabhu PR, Vivek Kumar RK. Jeevanram. DNA damage and integrity of UV-induced DNA repair in lymphocytes of smokers analysed by the comet assay. Mutat Res 520: 179-187, 2002   DOI
13 Aldini G, Yeum KJ, Russell RM, Krinsky NI. A method to measure the oxidizability of both aqueous and lipid compartments of plasma. Free Radic Biol Med 31: 143-1050, 2001   DOI   ScienceOn
14 Jeon EJ, Kim JS, Park YK, Kim TS, Kang M-H. Protective effect of yellow-green vegetables juices on DNA damage in chinese hamster lung cell using comet assay. Korean J Nutr 36(1): 24-31, 2003
15 Ministry of Health and Welfare. Report on 2001 National Health and Nutrition Survey, 2002
16 Kang MH, Park YK. Selection of antioxidative biomarkers in human intervention trials through literature review and the expert survey. Proceedings of 2005 The Korean Nutrition Society Annual Meeting & International Symposium, P2-38: 232, Gyeongju, Korea, Nov. 3-5th, 2005
17 Omenn GS, Goodman GE, Thornquist MD, Balmes J, Cullen MR, Glass A, Keogh JP, Meyskens FL Jr., Valanis B, Williams JH Jr., Barnhart S, Cherniack MG, Brodkin CA, Hammar S. Risk factors for lung cancer and for intervention effects in CARET, the Beta- Carotene and Retinol Efficacy Trial. J Natl Cancer Inst 88: 1550- 1559, 1996   DOI   ScienceOn
18 Myung-Hee Kang, Jung-Shin Kim, Yoo Kyoung Park, Tae Seok Kim. The effect of green Juice supplementation on antioxidant defecse system and lymphocytes DNA damage in middle-aged Korean smokers. Genetics and Molecular Biology, Supplement (4th ICEMHP, Brazil) 26(2): 71, 2003
19 Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in men. J Mol Med 74: 297-312, 1996   DOI
20 Rice-Evans C, Miller N. Antioxidant status in plasma and body fluids. Meth Enzymol 234: 279-293, 1994   DOI
21 Park YK, Park E, Kim JS, Kang MH. Daily grape juice consumption reduces oxidative DNA damages and plasma free radical levels in healthy Koreans, Mutation Research 529(1-2): 77-86, 2003   DOI
22 Singh RB, Niaz MA, Bishnoi I, Sharma JP, Gupta S, Rastogi SS, Singh R, Begum R, Chibo H, Shoumin Z. Diet, antioxidant vitamins, oxidative stress and risk of coronaty artery desease: The peerzada prospective study. Acta Cardiologica XLIX: 441-452, 1994
23 Randhir, Shetty P, Sheety K, L-DOPA and total phenolic stimulation of dark germinated fava bean in response to peptide and phytochemical elicitors. Process Biochemistry 37: 1247-1256, 2002   DOI   ScienceOn
24 Duthie SJ, Ma A, Ross MA, Collins AR. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res 56(6): 1291-1295, 1996
25 Lee BM, Lee SK, Kim HS. Inhibition of oxidative DNA damage, 8-OHdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, beta-carotene and red ginseng). Cancer Lett 132(1-2): 219-227, 1998   DOI   ScienceOn
26 O'Byrun DJ, Deraraj S, Grundy SM, Ialal I. Comparison of the antioxidant dffect of concord grape juice flavonoids and ${\alpha}$-tocopherol on markers of oxidative stress in healthy adults. Am J Clin Nutr 76: 1367-1374, 2002   DOI
27 Rojas E, Lopez MC, Valverde M. Single cell gel electrophoresis assay: methodology and applications. Journal of Chromatography 722: 225-254, 1999   DOI   ScienceOn
28 Muller H, Bub A, Watzl B, Rechkemmer G. Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich food. Eur J Nutr 38(1): 35-44, 1999   DOI
29 Brown KM, Morrice PC, Duthie GG. Vitamin E supplementation suppresses indexes of lipid peroxidation concentration remain unchanged. Am J Clin Nutr 60(3): 383-387, 1994   DOI
30 Okuyama T, Takata M, Takayasu J, Hasegawa T, Tokuda H, Nishino A, Nishino H, Iwashima A. Anti-tumor-promotion by principles obtained from Angelica keiskei. Planta Med 57(3): 242- 246, 1991   DOI   ScienceOn
31 The ${\alpha}$-Tocopherol, ${\beta}$-Carotene Caner Prevention Study Group: The effect of vitamin E and ${\beta}$-carotene on the incidence of lung cancer and other cancers in male smokers. N Eng J Med 330: 1029-1035, 1994   DOI   ScienceOn
32 Bub A, Watzl B, Blockhaus M, Briviba K, Liegibel U, Muller H, Pool-Zobel BL, Rechkemmer G. Fruit juice consumption modulates antioxidative status, immune status and DNA damage. J Nutr Biochem 14: 90-98, 2003   DOI   ScienceOn
33 Sardas S, Karabiyik L, Aygun N, Karakaya AE. DNA damage evaluated by the alkaline comet assay in lymphocytes of humans anaesthesized with isofluorane, Mutat Res 418: 1-6, 1998   DOI   ScienceOn
34 Gey KF, Brubacher GB, Stahelin HB. Plasma levels of antioxidant vitamins in relation to ischemic heart disease and cancer. Am J Clin Nutr 45: 1368-1377, 1987   DOI
35 Park YK, Kim YA, Park EJ, Kim JS, Kang MH. Estimated flavonoids intake in Korean adults using semiquantitative foodfrequency questionnaire. Korean J Nutr 35(10): 1081-1088, 2002   과학기술학회마을
36 Pesce AJ, Kaplan LA. Methods in clinical chemistry. The C.V. Mosby Company, 1987
37 Park EJ, Kang MH. Application of the alkaline comet assay for detecting oxidative DNA damage in human biomonitoring. Korean J Nutrition 35(2): 213-222, 2002
38 Hakim IA, Harris RB, Brown S, Sherry Chow H-H, Wiseman S, Agarwal S, Talbot W. Effect of increased tea consumption on oxidative DNA damage among smokers: A randomized controlled study. J Nutr 133: 3303S-3309S, 2003
39 Collins AR, Harrington V, Drew J, Melvin R. Nutritional modulation of DNA repair in a human intervention study. Carcinogenesis 24(3): 511-515, 2003   DOI   ScienceOn
40 Pryor WA, Stone K. Oxidants in cigarette smoke: radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann NY Acad Sci 686: 12-18, 1993   DOI
41 Lee SM, Park KY, Lee SH. Antimutagenic effect and active compound analysis of kale juice in salmonella assay system. Korean J Food Sci Technol 26(5): 965-971, 1997   과학기술학회마을
42 Pamuk ER, Byer T, Coates RJ, Vann JW, Sowell AL, Gunter EW, Glass D. Effect of smoking on serum nutrient concentrations in African-American women. Am J Clin Nutr 59: 891-895, 1994   DOI
43 Diplock AT. Antioxidants and disease prevention. Mol Aspects Med 15(4): 293-376, 1994   DOI   ScienceOn
44 Jeon SS, Park JC, Kim SH, Lee DY, Choi HM, Hwang EY. Changes in biologically active component of Angelica keiskei by cooking methods. J Korean Soc Food Sci Nutr 27(1): 121-125, 1998   과학기술학회마을