• Journal of Microbiology and Biotechnology
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• 제23권3호
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• pp.405-413
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• 2013

Monocyte chemotactic protein-3 (MCP-3), a chemokine that is in a superfamily of structurally related small chemotactic cytokines involved in leukocyte trafficking, is regarded as a key factor in atherogenesis. In this study, we examined the changes in atherogenic parameters including hepatic lipid accumulation and oxidative balance in MCP- 3-overexpressing transgenic mice (MCP-3 mice) under atherogenic conditions. To induce an extreme atherogenic condition, mice were fed a high-fat, high-cholesterol (HFHC) diet for 12 weeks. The body weight and food intake were not changed by MCP-3 overexpression in the aorta. On a HFHC diet, the MCP-3 mice had higher plasma levels of total cholesterol and a higher atherogenic index compared with wild-type mice, although there were no differences in the plasma HDL-cholesterol and triglyceride levels. Furthermore, an increase in lipid accumulation was observed in the aortas as well as the livers of the HFHC diet-fed MCP-3 mice compared with wild-type mice. The activities of antioxidant enzymes increased in the livers of the HFHC diet-fed MCP-3 mice, whereas supplementation with antioxidants, naringin and hesperidin, reversed the activities of the hepatic antioxidant enzymes in HFHC diet-fed MCP-3 mice, indicating that there might be more oxidative damage to the tissues in the HFHC diet-fed MCP-3 mice leading to progression towards atherosclerosis and hepatic steatosis. Microarray analyses of the aorta revealed atherosclerosis-, PPARs-, lipoprotein receptor, and apolipoprotein-related genes that were affected by the HFHC diet in MCP-3 mice. These findings suggest that aortic MCP-3 overexpression may contribute to the development of atherosclerosis and hepatic steatosis under atherogenic conditions.

• 대한공간정보학회지
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• 제14권3호
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• pp.87-95
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• 2006

최근 홍수피해의 문제점을 분석해 보면 기상 이변으로 집중호우가 발생하고 특히, 국지적인 호우에 의한 돌발홍수(flash flood)로 인한 피해가 빈번하게 발생하고 있다. 본 연구에서는 하천 지역의 돌발홍수의 특성을 밝혀 보고자 GIS 기법을 이용하여 하천 유역의 수문학적 돌발홍수 예측모형을 지형기후학적순간단위유량도를 적용하여 구축하였다. DB는 GIS를 사용하여 구축하였으며 DEM으로부터 유역과 하천을 추출하였다. 연구지역의 하천은 소, 중, 대규모 유역을 포함하였다. 돌발홍수경보에 관한 기준설정을 위해 첨두홍수량과 한계유량을 결정하였다. 한계유량은 지형기후학적 단위유량도 모델로 계산되었고 지형인자와의 적합도를 분석하였다.

• Journal of Ginseng Research
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• 제37권2호
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• pp.187-193
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• 2013

The effect of Korean red ginseng (KRG) on diabetic renal damage was investigated using streptozotocin (STZ)-induced diabetic rats. The diabetic rats showed loss of body weight gain, and increases in kidney weight and urine volume, whereas the oral administration of KRG at a dose of 100 or 250 mg/kg of body weight per day for 28 d prevented these diabetes-induced physiological abnormalities. Among the kidney function parameters, elevated plasma levels of urea nitrogen and creatinine in diabetic control rats tended to be lowered in KRG-treated rats. In addition, administration of KRG at a dose of 100 mg/kg body weight in the diabetic rats showed significant decreases in serum glucose and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), implying that KRG might prevent the pathogenesis of diabetic complications caused by impaired glucose metabolism and oxidative stress. KRG also significantly reduced advanced glycation end product (AGE) formation and secretion from kidney of diabetic rats. Furthermore, KRG decreased the levels of N-(carboxymethyl) lysine and expression of AGE receptor. KRG also reduced the overexpression of cyclooxygenase-2 and inducible nitric oxide synthase in the kidney via deactivation of nuclear factor-kappa B. We also found that KRG prevented STZ-induced destruction of glomerular structure and significantly suppressed high glucose-induced fibronectin production. Taken together, KRG ameliorates abnormalities associated with diabetic nephropathy through suppression of inflammatory pathways activated by TNF-${\alpha}$ and AGEs. These findings indicate that KRG has a beneficial effect on pathological conditions associated with diabetic nephropathy.

• Journal of Ginseng Research
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• 제41권4호
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• pp.503-512
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• 2017

Background: Chronic heavy alcohol consumption may raise the risk of developing type 2 diabetes mellitus. Saponins inhibit apoptosis of pancreatic islet cells and reduce lipid parameters. The present study was designed to investigate the effect of saponin on chronic ethanol-treated diabetic rats. Methods: Long-Evans Tokushima Fatty (LETO) and Otsuka Long-Evans Tokushima Fatty (OLETF) rats were pair-fed a Lieber-DeCarli diet with and without 5% ethanol for 12 wks. Two weeks after starting the pair-feeding with the Lieber-DeCarli diet, intraperitoneal injection of saponin was performed for 10 wks. To perform the experiments, rats were divided as follows: LETO-Control (LC), LETO-Ethanol (LE), LETO-Ethanol-Saponin (LES), OLETF-Control (OC), OLETF-Ethanol (OE), and OLETF-Ethanol-Saponin (OES). Results: The weights of epididymal and mesenteric fat tissue in LES and OES rats were the lightest from among the LETO and OLETF groups, respectively. The secretion of alanine aminotransferase and cholesterol in OES rats decreased significantly compared to their secretion in OC and OE rats, respectively. The islets of the pancreas in LE and OE rats showed clean, unclear, and smaller morphology compared to those of LC, LES, OC, and OES rats. In addition, the expression of insulin in the islets of the pancreas in LC, LES, OC, and OES rats was higher than in LE and OE rats. Conclusion: Saponin may not only be helpful in alleviating the rapid progress of diabetes due to chronic alcohol consumption in diabetic patients, but may also show potential as an antidiabetic drug candidate for diabetic patients who chronically consume alcohol.

• 콘크리트학회논문집
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• 제17권6호
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• pp.871-878
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• 2005

화재에 노출된 철근콘크리트 기둥은 기둥을 구성하는 재료물성의 변화와 함께 기둥의 구조적 성능에도 결과적으로 열화현상을 나타내게 된다. 기둥의 구조적 성능의 열화정도는 화재동안 기둥단면에 전달된 열의 양과 관련이 있다. 이를 예측하기 위한 합리적인 열 전달 모델은 기둥이 가열을 받는 동안 전달된 열 정도에 따라 변하는 콘크리트의 전도율 및 열용량, 그리고 단면 내 잉여수 증발에 대한 적절한 고려를 하여야 한다. 본 연구에서 제안한 모델은 양해법 유한차분법에 근거하여 개발되었으며 기존에 제안된 모델들에 비하여 이들 모든 변수들의 영향을 적절하게 고려하고 있다. 본 연구에서 개발된 모델은 기존 화해 실험에 의한 기둥 단면 내 온도를 적절하게 예측하였다.

• 한국환경보건학회지
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• 제43권5호
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• pp.360-369
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• 2017

Objectives: The organizational culture of small-scale enterprises is influenced by a lack of awareness of industrial accidents and a business-oriented culture. Therefore, the purpose of this study is to establish a correlation between chemical awareness and worker safety, which is the reason why a company's safety environment is responsible for human factor accidents. Methods: The research model established the organizational culture, human relationships, organizational relationships, workplace culture, safety climate, and safety parameters, all of which affect the perception of chemical substances, then established safety rankings, communication, safety regulations, and safety support. Results: In order to derive the results of this study, the satisfaction level was mostly derived from the degree of engagement (+) depending on the number of years worked, in the hierarchical regression analysis. During work with chemicals, the number of years spent on the level of the service was determined by the influence of the chemical (-). Based on this company, the occurrence of chemical accidents caused by chemical materials was felt to be felt at the risk of causing the hazard to be felt, the absence of physical damage was excluded from the variables. The other variables detected in the variables were $62.8{\pm}24.4$. Conclusion: As a result, it is necessary to develop a statistical model for the verification of the hierarchical regression analysis, and the relationship between safety and awareness of the dangers of chemicals and biological agents requires periodic safety training. Job satisfaction was found to be higher if the level of awareness was sufficient.

• Journal of Ecology and Environment
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• 제29권5호
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• pp.479-484
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• 2006

Ecophysiological parameters of non-transgenic sweetpotato (NT) and transgenic sweetpotato (SSA) plants were compared to evaluate their resistance to multiple environmental stresses. Stomatal conductance and transpiration rate in NT plants decreased markedly from Day 6 after water was withheld, whereas those values in SSA plants showed relatively higher level during this period. Osmotic potential in SSA plants was reduced more negatively as leaf water potential decreased from Day 8 after dehydration treatment, while such reduction was not shown in NT plants under water stressed condition. SSA plants showed less membrane damage than in NT plants. As water stress and high light stress, were synchronously applied to NT and SSA plants maximal photochemical efficiency of PS II ($F_v/F_m$) in NT plants markedly decreased, while that in SSA plants was maintained relatively higher level. This trend of changes in $F_v/F_m$ between SSA plants and NT plants was more conspicuous as simultaneously treated with water stress, high light and high temperature stress. These results indicate that SSA plants are more resistive than NT plants to multiple environmental stresses and the enhanced resistive characteristics in SSA plants are based on osmotic adjustment under water stress condition and tolerance of membrane.

• 한방재활의학과학회지
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• 제26권3호
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• pp.1-15
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• 2016

Objectives This study was designed to evaluate the anti-inflammatory effects of Seungseup-tang (SST) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods Osteoarthritis was induced by injection of MIA ($50{\mu}l$ with 80 mg/ml) into knee joint cavity of rats. Rats were divided into 4 groups (normal group, control group, indomethacin treated group, SST treated group, each n=6). Normal group was not injected with MIA and taken normal diet. Control group was injected with MIA and taken with distilled water. Indomethacin treated group was injected with MIA and taken indomethacin 5 mg/kg by oral administration. SST treated group was injected with MIA and taken SST 200 mg/kg by oral administration. We examined the weight-bearing ability of hind paw, biomarkers related to oxidative stress in serum, inflammatory proteins and inflammatory mediators and cytokines. Moreover, histopathological examination of knee joint structure was also performed by Hematoxylin & Eosin (H&E), Safranin-O staining method. Results In the present study, SST treated group showed a similar inhibitory effects alike indomethacin treated group, in most of the studied parameters of inflammation. The increased oxidative stress biomarker such as reactive oxygen species (ROS) and peroxy nitrite ($ONOO^-$) in the serum were reduced with SST. Especially, the level of $ONOO^-$ compared with control group significantly suppressed. Also, the expression of inflammatory mediators and cytokines induced by nuclear factor-kappa B (NF-${\kappa}B$) activation was modulated through inhibition of IkBa phosphorlation. In addition, histological analysis revealed that cartilage damage by MIA repaired markedly in SST treated group. Conclusions According to the results, Seungseup-tang may be effective for preventing the progression of osteoarthritis.

• 한국지진공학회논문집
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• 제12권3호
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• pp.37-44
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• 2008

본 연구에서는 확률적 지진취약도 분석방법을 3경간 FCM 교량에 적용하여 지반특성에 따른 지진취약 교각의 위치와 교각 상 하부에서의 소성힌지 발생 상태를 수치예제 결과의 분석을 통해 평가하였다. 이를 위해, 취약도 곡선을 최대지반가속도(PGA)의 변수에 대한 대수정규분포 함수로 가정하고 대수정규분포 함수의 주요 계수인 중앙값과 대수표준편차는 최우도추정법(Maximum Likelihood Method)으로 구하였다. 또한 지점별 상이한 지반특성은 "도로교표준시방서"에 제시되어 있는 지반의 등가 스프링을 사용하여 해석모델에 반영하였으며, 지진취약도 분석에 필요한 구조물의 손상지수로 교각의 소성힌지에서의 회전연성도를 이용하였다.

• Earthquakes and Structures
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• 제9권3호
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• pp.481-501
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• 2015

Current design codes and technical recommendations often provide rough indications on how to assess effective stiffness of Reinforced Concrete (R/C) frames subjected to seismic loads, which is a key factor when a linear analysis is performed. The Italian design code (NTC-2008), Eurocode 8 and ACI 318 do not take into account all the structural parameters affecting the effective stiffness and this may not be on the safe side when second-order $P-{\Delta}$ effects may occur. This paper presents a study on the factors influencing the effective stiffness of R/C beams, columns and walls under seismic forces. Five different approaches are adopted and analyzed in order to evaluate the effective stiffness of R/C members, in accordance with the scientific literature and the international design codes. Furthermore, the paper discusses the outcomes of a parametric analysis performed on an actual R/C building and analyses the main variables, namely reinforcement ratio, axial load ratio, concrete compressive strength, and type of shallow beams. The second-order effects are investigated and the resulting displacements related to the Damage Limit State (DLS) under seismic loads are discussed. Although the effective stiffness increases with steel ratio, the analytical results show that the limit of 50% of the initial stiffness turns out to be the upper bound for small values of axial-load ratio, rather than a lower bound as indicated by both Italian NTC-2008 and EC8. As a result, in some cases the current Italian and European provisions tend to underestimate second-order $P-{\Delta}$ effects, when the DLS is investigated under seismic loading.