• Journal of Positioning, Navigation, and Timing
• /
• 제6권3호
• /
• pp.105-116
• /
• 2017

Several wireless localization algorithms are evaluated for the IR-UWB-based indoor location with the assumption that the ranging measurements contain the channelwise Non-Calibration Error (NCE). The localization algorithms can be divided into the Model-free Localization (MfL) methods and Model-based Kalman Filtering (MbKF). The algorithms covered in this paper include Iterative Least Squares (ILS), Direct Solution (DS), Difference of Squared Ranging Measurements (DSRM), and ILS-Common (ILS-C) methods for the MfL methods, and Extended Kalman Filter (EKF), EKF-Each Channel (EKF-EC), EKF-C, Cubature Kalman Filter (CKF), and CKF-C for the MbKF. Experimental results show that the DSRM method has better accuracy than the other MfL methods. Also, it demands smallest computation time. On the other hand, the EKF-C and CKF-C require some more computation time than the DSRM method. The accuracy of the EKF-C and CKF-C is, however, best among the 9 methods. When comparing the EKF-C and CKF-C, the CKF-C can be easily used. Finally, it is concluded that the CKF-C can be widely used because of its ease of use as well as it accuracy.

• Molecules and Cells
• /
• 제40권7호
• /
• pp.450-456
• /
• 2017

Mammalian physiology and behavior are regulated by an internal time-keeping system, referred to as circadian rhythm. The circadian timing system has a hierarchical organization composed of the master clock in the suprachiasmatic nucleus (SCN) and local clocks in extra-SCN brain regions and peripheral organs. The circadian clock molecular mechanism involves a network of transcription-translation feedback loops. In addition to the clinical association between circadian rhythm disruption and mood disorders, recent studies have suggested a molecular link between mood regulation and circadian rhythm. Specifically, genetic deletion of the circadian nuclear receptor Rev-$erb{\alpha}$ induces mania-like behavior caused by increased midbrain dopaminergic (DAergic) tone at dusk. The association between circadian rhythm and emotion-related behaviors can be applied to pathological conditions, including neurodegenerative diseases. In Parkinson's disease (PD), DAergic neurons in the substantia nigra pars compacta progressively degenerate leading to motor dysfunction. Patients with PD also exhibit non-motor symptoms, including sleep disorder and neuropsychiatric disorders. Thus, it is important to understand the mechanisms that link the molecular circadian clock and brain machinery in the regulation of emotional behaviors and related midbrain DAergic neuronal circuits in healthy and pathological states. This review summarizes the current literature regarding the association between circadian rhythm and mood regulation from a chronobiological perspective, and may provide insight into therapeutic approaches to target psychiatric symptoms in neurodegenerative diseases involving circadian rhythm dysfunction.

• 세라미스트
• /
• 제23권2호
• /
• pp.184-199
• /
• 2020

The solid oxide fuel cells (SOFCs) are the one of the most promising energy conversion devices which can directly convert chemical energy into electric power with high efficiency and low emission. The lowering operating temperature below 800 ℃ has been considered as the mostly considerable research and development for commercialization. The major issue is to maintain reasonably high performance of SOFCs at reduced temperatures due to increment of polarization resistance of electrodes and electrolyte. Thus, the alternative materials with high catalytic activities and fast oxygen ion conductivity are required. For recent advances in electrolyte materials and technology, newly designed, highly conductive electrolyte materials and structural engineering of them provide a new path for further reduction in ohmic polarization resistance from electrolytes. Here, a powerful strategy of the bilayer concept with various oxide electrolytes of SOFCs are briefly reviewed. These recent developments also highlight the need for electrolytes with greater conductivity to achieve a high performance, thus providing a useful guidance for the rational design of cell structures for SOFCs. Moreover, cell design, materials compatibility, processing methods, are discussed, along with their role in determining cell performance. Results from state-of-the-art SOFCs are presented, and future prospects are discussed.

• Molecules and Cells
• /
• 제42권5호
• /
• pp.418-425
• /
• 2019

Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.

• Biomolecules & Therapeutics
• /
• 제29권1호
• /
• pp.41-51
• /
• 2021

Src family kinases (SFKs), an important group of non-receptor tyrosine kinases, are suggested to be excessively activated during various types of tissue fibrosis. The present study investigated the effect of KF-1607, an orally active and a newly synthesized Src kinase inhibitor (SKI) with proposed low toxicity, in preventing the progression of renal interstitial fibrosis. Unilateral ureteral obstruction (UUO) surgery was performed in 6-week-old male C57BL/6 mice to induce renal interstitial fibrosis. Either KF-1607 (30 mg/kg, oral gavage) or PP2 (2 mg/kg, intraperitoneal injection), a common experimental SKI, was administered to mice for seven days, started one day prior to surgery. UUO injury-induced SFK expression, including Src, Fyn, and Lyn kinase. SFK inhibition by KF-1607 prevented the progression of tubular injury in UUO mice, as indicated by decreases in albuminuria, urinary KIM-1 excretion, and kidney NGAL protein expression. Renal tubulointerstitial fibrosis was attenuated in response to KF-1607, as shown by decreases in α-SMA, collagen I and IV protein expression, along with reduced Masson's trichrome and collagen-I staining in kidneys. KF-1607 also inhibited inflammation in the UUO kidney, as exhibited by reductions in F4/80 positive-staining and protein expression of p-NFκB and ICAM. Importantly, the observed effects of KF-1607 were similar to those of PP2. A new pan Src kinase inhibitor, KF-1607, is a potential pharmaceutical agent to prevent the progression of renal interstitial fibrosis.

• 대한의용생체공학회:의공학회지
• /
• 제42권3호
• /
• pp.125-142
• /
• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• 한국콘텐츠학회논문지
• /
• 제21권5호
• /
• pp.139-148
• /
• 2021

이 연구는 온라인 데이터를 활용해 대구·경북 행정통합에 대한 태도와 반응을 살펴보았다. 구체적으로 온라인 기사와 유튜브 영상의 댓글을 통하여 알아보았다. 2020년 1월 1일부터 10월 18일까지 수집한 데이터는 대구경북행정통합공론화위원회 출범 이전과 이후로 나누어 분석했다. 그 결과 다음과 같은 내용을 확인했다. 첫째, 댓글 유형을 시기별로 비교했을 때 공론화위원회 이후 회의론자는 줄어들었고 신념주의자는 증가했다. 둘째, 행정통합에 대해 낙관적 태도를 보여주는 단어를 공론화위원회 출범 이후 확인했다. 셋째, 하위집단분석 결과 이전과 이후 모두 행정통합에 대한 구체적 절차나 방향을 언급하는 단어군이 보이지 않았다. 이는 아직까지 행정통합과 관련한 충분한 정보나 토론논제가 제공되지 않았음을 보여준다. 이 연구에서는 정책당국이 놓칠 수 있는 숨겨진 여론을 찾도록 도와주고, 필요시 즉각적 대응을 할 수 있도록 시사점을 제공하고자 한다.

• Journal of Electrochemical Science and Technology
• /
• 제15권1호
• /
• pp.198-206
• /
• 2024

Given the high theoretical capacity (1,675 mAh g^-1) and the inherent affordability and ubiquity of elemental sulfur, it stands out as a prominent cathode material for advanced lithium metal batteries. Traditionally, sulfur was sequestered within conductive porous carbons, rooted in the understanding that their inherent conductivity could offset sulfur's non-conductive nature. This study, however, pivots toward a transformative approach by utilizing diatom shell (DS, diatomite)-a naturally abundant and economically viable siliceous mineral-as a sulfur host. This approach enabled the development of a sulfurlayered diatomite/S composite (DS/S) for cathodic applications. Even in the face of the insulating nature of both diatomite and sulfur, the DS/S composite displayed vigorous participation in the electrochemical conversion process. Furthermore, this composite substantially curbed the loss of soluble polysulfides and minimized structural wear during cycling. As a testament to its efficacy, our Li-S battery, integrating this composite, exhibited an excellent cycling performance: a specific capacity of 732 mAh g^-1 after 100 cycles and a robust 77% capacity retention. These findings challenge the erstwhile conviction of requiring a conductive host for sulfur. Owing to diatomite's hierarchical porous architecture, eco-friendliness, and accessibility, the DS/S electrode boasts optimal sulfur utilization, elevated specific capacity, enhanced rate capabilities at intensified C rates, and steadfast cycling stability that underscore its vast commercial promise.

• 한국경제지리학회지
• /
• 제17권4호
• /
• pp.702-717
• /
• 2014

이 연구는 대구 경북에 거주하는 가구를 대상으로 가구특성별로 주거입지 선호요인을 분석하는 것을 목적으로 한다. 이러한 연구목적 하에서 첫째로, 대구 경북지역 가구의 주거입지 요인 선호도에 대한 설문을 실시하고, 둘째로, 설문결과를 토대로 한 AHP 분석을 통해 가구특성별 주거입지 선호요인의 특징을 분석하고, 마지막으로 대구 경북지역 가구의 주거입지 전략에 대한 시사점을 도출하였다. 연구결과를 요약하면 다음과 같다. 첫째로, 대구 경북 가구들이 중요하게 고려하는 주거입지 요인으로는 향후 시세차익, 생활환경, 대중교통 접근성, 자가용 교통 접근성, 안전성 순으로 나타난다. 따라서 대구 경북 가구는 주거입지 선정 시 주택의 투자가치와 생활여건을 동시에 고려한다는 것을 볼 수 있다. 둘째로, 연령별 AHP 분석결과를 보면, 30대 이하는 접근성 요인에서, 40대는 향후 시세차익과 학군 요인에서, 50대 이상은 생활환경과 자연환경, 안전성 요인에서 상대적으로 높은 선호도를 보인다. 셋째로, 가구소득별 AHP 분석결과를 보면, 3천만원 미만의 저소득층은 생활환경과 접근성 요인에서, 3천만~6천만원의 중간소득층은 향후 시세차익과 접근성 요인에서, 6천만원 이상의 고소득층은 안전성, 자가용교통 접근성, 조망권/일조권 요인에서 상대적으로 높은 선호도를 보인다. 따라서 대구 경북 가구들은 연령과 가구소득에 따라 선호하는 입지요인이 차별적으로 나타나고 있음을 확인할 수 있다.

• 한국지역지리학회지
• /
• 제22권1호
• /
• pp.168-179
• /
• 2016

이 논문은 대구 경북지역에 거주하는 북한이탈 초등학생의 문화변용과 학교생활 만족도를 분석하였다. 분석결과, 대구 경북지역에 거주하는 북한이탈 초등학생의 속성은 전국 혹은 수도권과는 차이가 있었으며, 이러한 차이는 문화변용에 영향을 미치는 것으로 나타났다. 문화변용에 있어서는 통합형보다는 동화형의 비중이 약간 크지만 한국 문화를 수용하는데 소극적인 분리형과 주변화형의 비율이 42%를 차지하여 국내생활에 많은 어려움을 겪고 있다고 볼 수 있다. 그리고 일반적으로 나이가 들고 학교생활에 익숙할수록 남한문화를 적극적으로 수용하려는 경향이 강하지만 북한에서 태어난 학생의 경우는 남한문화를 거부하려는 경향이 상대적으로 강한 것으로 나타났다. 학교생활만족도는 대체로 만족하는 편이며, 영역별로는 교사와의 관계와 학교환경의 만족도는 높은 반면에 학습활동에 대한 만족도는 가장 낮았다. 또한 학교생활만족도와 문화변용 유형간의 관계에 있어서는 새로운 문화를 적극적으로 수용하려는 유형의 경우가 상대적으로 만족도가 높았다. 결론적으로 탈북한 국내이주 청소년에 대한 기존의 획일적이고 단기적인 교육위주의 지원정책에서 탈피하여, 문화변용차원의 계층별 속성별 수요를 반영한 체계적이고 장기적인 지원정책으로 패러다임을 전환할 필요가 있다.