• Asian-Australasian Journal of Animal Sciences
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• 제16권4호
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• pp.534-540
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• 2003

This study was conducted to investigate the effect of supplementation of galacto-oligosaccharides (GOS) on methane emission, nutrient digestibility, energy utilization and blood metabolites by Holstein cows fed silages. In two sequential digestion and respiratory trials, two non-lactating Holstein cows were arranged to a balanced incomplete block design. Experimental diets consisted of two silage types; orchardgrass (Dactylis glomerata L.) based silage (OS), mixed silage (orchardgrass based silage and alfalfa (Medicago sativa L.) silage) (MS), while two GOS levels were without supplementation (0) and 2% of dry matter intake supplementation (2). Four combination diets were OS-0, OS-2, MS-0 and MS-2. Significant effects of silage types and GOS supplementation levels were not observed for DM and OM intake. Whereas the digestibility of OM, NDF and ADF was significantly (p<0.05) higher in cows fed OS with and without GOS compared cows fed MS diets. As percentage of GE intake, fecal energy loss for OS diets was significantly (p<0.05) declined than for MS diets. In contrast, cows fed MS diets had lower (p<0.05) urine energy loss as a proportion of GE intake compared to OS diets. Energy loss as CH4 and heat production was numerically increased when cows fed both OS and MS with GOS supplementation. Compared to OS, CH4 emission in cows fed MS was numerically decreased by 10.8 %. Methane conversion ratio (energy loss as CH4 per unit of GE intake) for OS-0, OS-2, MS-0 and MS-2 were 7.1, 7.2, 6.8 and 7.0, respectively. Plasma of glucose and urea-N concentration were significantly (p<0.05) elevated from 1 h to 6 h after feeding, otherwise total protein in plasma was declined (p<0.01) at 6 after feeding.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.995-1002
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• 2008

Kura clover (Trifolium ambiguum M. Bieb.) is a rhizomatous perennial legume that has potential as a forage crop in the North-Central USA because of its excellent persistence under environmental extremes. Little information is available about defoliation effects on productivity of mixtures of kura clover with grasses typically grown in this region. Two field trials were conducted to evaluate the effects of defoliation management on yield and species composition of binary mixtures of 'Rhizo' kura clover with 'Comet' orchardgrass (Dactylis glomerata L.), 'Badger' smooth bromegrass (Bromus inermis Leyss.), 'Park' Kentucky bluegrass (Poa pratensis L.), and solo-seeded kura clover near Arlington, WI. Three harvest schedules (three, four, or five times annually) and two cutting heights (4 or 10 cm) were imposed. Infrequent defoliation and lower cutting height produced significantly greater total forage yield, 6.6, 5.8, and 5.2 Mg/ha in 3-, 4-, and 5-harvest systems, respectively; and 6.5 and 5.2 Mg/ha for the 4- and 10-cm cutting height, respectively. Averaged over 3 yr and two environments, mixtures had higher forage productions than solo kura clover (6.3, 5.7, and 6.0 Mg/ha for the Kentucky bluegrass, orchardgrass, and smooth bromegrass mixtures, respectively; compared to 5.2 Mg/ha for solo kura clover). The proportion of kura clover in mixtures increased from yr 1 to yr 2 and was constant from yr 2 to yr 3 (34, 58, and 57%, respectively). We conclude that kura clover has excellent potential as a long-term component of grass-legume mixtures regardless of the cutting height, harvest frequency or grass species, even though the proportion of kura clover in harvested forage was significantly greater with less frequent harvest and shorter cutting height of all mixtures.

• 한국작물학회지
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• 제49권3호
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• pp.232-236
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• 2004

오차드그래스의 최적 조직배양조건을 확립하기 위하여 'Roughrider' 품종의 성숙종자로부터 최적 배발생 캘러스 유도 및 식물체 재분화에 미치는 배지첨가물질의 영향을 조사하였다. 성숙종자로부터 배발생 캘러스 유도시 첨가되는 생장조절 물질로는 3 mg/L 2,4-D가 가장 효율적이었으며, 식물체 재분화에는 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 배지에 캘러스를 배양했을 때 36.3%의 재분화율을 나타내었다. 캘러스 유도배지와 재분화배지에 1 g/L의 casein hydrolysate와 300 mg/L의 proline을 동시에 첨가해주었을 때 캘러스 유도율과 재분화율이 각각 57.3%와 60.7%로 증가되었다. 또한 항산화물질로서 10 mg/L의 $\textrm{AgNO}_3$ 와 40 mg/L의 cysteine을 첨가해준 과 켈러스 유도율 재분화율 각각 68.7%와 71.7%까지 증가되었다. 본 연구를 통하여 확립된 성숙종자로부터 효율적인 배발생 캘러스의 유도 및 식물체 재분화 체계는 유전자 형질전환을 통한 신품종 오차드그래스 개발에 유용하게 응용되어질 수 있을 것이다.

• 한국초지조사료학회지
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• 제23권2호
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• pp.107-114
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• 2003

Growth characteristics and seed productivities of orchardgrass "Hwabsung 2 Ho" cultivated for seed production were examined in the NLRI from 2000 to 2002. The experimental design was a split-plot arrangement with three replications. The main-plots were three drill widths of 15, 30 and 45cm, and subplots were four nitrogen application levels of 45, 90, 135 and 180kg/ha in early spring. Lodging was less in 45cm of drill width than in 15 and 30cm and was not severe by 90kg per ha of nitrogen including 45cm of drill width. Number of panicles per $1\m^2$ was tended to be more in 15cm of drill width than in 30 or 45cm. Among 45cm of drill widths. 45kg per ha of nitrogen had most numerous panicles. Panicle length, number of 1st branch, weight of 1,000 grain, and germination of 30 or 45cm of drill width showed better characters than that of 15cm. Seed yield was not statistical different among the treatments but was some more in 30cm of drill width, and was tended not to be different among nitrogen levels. Therefore, considering lodging tolerances, seed yield components, and mechanizations, treatment with 45cm of drill width including 45kg per ha of nitrogen in early spring was better than other treatments for orchardgrass seed production.

• 한국초지조사료학회지
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• 제23권2호
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• pp.101-106
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• 2003

본 연구는 오차드그라스 종자생산을 위한 적정 파종시기를 구명하기 위하여 축산기술연구소 초지사료과 시험포장에서 2001년부터 2002년까지 실시되었다. 공시품종은 국내에서 육성한 오차드그라스 "합성2호"로 하였다. 처리내용은 파종시기로 8월 10, 20, 30일과 9월 10, 20, 30일에 하였다. 시험구 배치는 난괴법 3반복으로 하였다. 정착상태는 8월 10일 파종에서는 늦 장마로 인하여 유모의 정착이 불량하였다. 수량구성요소 중 단위면적당 이삭수는 8월 20일 파종에서 $265/\textrm{m}^2$로 가장 많았고, 파종기가 늦을수록 이삭수가 줄었으며, 9월 20일 이후 파종은 출수경이 거의 없었다. 수량구성요소들은 8월 20일 파종 이후부터 파종기가 늦어질수록 점차 불량하였다. 그에 따라서 종자수량도 8월 20일 파종에서 759kg/ha로 가장 많았고, 파종이 늦어질수록 유의적으로 감소하였다.적으로 감소하였다.

• 한국초지조사료학회지
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• 제5권1호
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• pp.33-36
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• 1985

牧場에 林間栽培함에 있어서 閉度의 多少가 牧草의 生育 및 收量에 미치는 影響을 究明코자 리기데타 人工造林地의 閉度를 0(露光區), 25, 50, 75%로 調節하여 Orchard grass를 栽培하였던 바 그 結果는 다음과 같다. 1 林地內의 Orchard grass의 葉面積은 各 刈取時期 모두 閉度 25, 0, 50, 75%區의 順位이며 特히 閉度 25%區는 0%區에 비하여 有意的인 增加傾向 이었다. 2. Orchard grass의 草長은 閉度가 높을수록 伸長하는 傾向이나 閉度 0%區와 25%區 間에는 差異를 認定할 수 없었다. 3 閉度의 差異에 따른 草長과 葉面積의 關係는 負의 利關關係를 나타내었다. 4. 林地內의 Orchard grass 年間 總 乾物收量은 페도 25%구가 露光區 에 비하여 有意的인 增收效果가 있었으며 50%區는 露光區에 비하여 약간 減少하는 傾向을 나타내었으나 統計的인 有意性은 認定되지 않았다. 그러나 閉度 75%區는 露光區에 비하여 有意的인 減收를 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1267-1272
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• 2005

The effect of various moisture contents of fresh forage on the change in nitrogen (N) fractions, in vitro ruminal N degradability, and the subsequent N utilization of silage in sheep were evaluated. Orchardgrass (Dactylis glomerata L.) with high (HM, 76%), medium (MM, 65%) and low (LM, 40%) moisture contents were ensiled into silos of 120 L capacity for 120 days. A nitrogen balance trial was conducted using a 4${\times}$4 Latin square design consisting of four dietary treatments (i.e. fresh forage, HM, MM and LM silages) and four wethers. With respect to N fractions, fraction 1 (buffer solution soluble N), fraction 2 (buffer solution insoluble N-neutral detergent insoluble N), fraction 3 (neutral detergent insoluble N-acid detergent insoluble N), and fraction 4 (acid detergent insoluble N) were determined. The proportion of fraction 1 in silages tended to decrease, while the in vitro ruminal degradability of insoluble N increased (p<0.05) with lower moisture contents at ensiling. Consequently, nitrogen utilization in sheep tended to improve as the moisture content of ensiled grass was decreased, with a negative correlation (p<0.01) between urinary N and the in vitro ruminal degradability of insoluble N. The averaged N retentions for HM, MM, and LM silage treatments were 59, 73 and 79% of that for fresh forage, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.894-899
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• 2007

An experiment was conducted using a [6, 6-$^2H$]glucose isotope dilution method to determine the effects of plantain (Plantago lanceolata L.) on plasma glucose metabolism in sheep taken from a thermoneutral environment and exposed to a hot environment. The sheep were fed either mixed hay (MH) of orchardgrass (Dactylis glomerata L.) and reed canarygrass (Phalaris arundinacea L.) at a 60:40 ratio or MH and plantain (PL) at a 9:1 ratio in a crossover design for each 23-day period. In both dietary treatments the metabolizable energy (ME) and crude protein intake were designed to be isoenergetic and isoproteinous at around maintenance level. The sheep were taken from a thermoneutral environment ($20^{\circ}C$, 70% RH) and exposed to a hot environment ($28-30^{\circ}C$, 70% RH) for 5 days. The isotope dilution method using a single injection of [6, 6-$^2H$]glucose was performed on the $18^{th}$ day of the thermoneutral environment and on the $5^{th}$ day of heat exposure. Plasma glucose pool size was numerically lower (p = 0.26) during heat exposure on both dietary treatments, and numerically higher (p = 0.13) on the MH diet irrespective of environmental temperature. Plasma NEFA concentration (p = 0.01) and glucose turnover rate (p = 0.03) were decreased during heat exposure, but remained similar between diets. It could be concluded that, although no positive impact of plantain on glucose metabolism was found under the present experimental conditions (plantain constituted only 10% of basal diet), plantain herb is an alternative to MH for rearing sheep in both thermoneutral and hot environments.

• Current Research on Agriculture and Life Sciences
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• 제8권
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• pp.59-64
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• 1990

경북지방일대(慶北地方一帶)에 자생(自生)하는 산야초(山野草) 중(中) 목초자원(牧草資源)으로서의 활용가치(活用價値)가 높은 자원(資源)을 수집(蒐集)하였다. 수집(蒐集)된 목초자원(牧草資源)은 본(本) 대학(大學) 포장(圃場)에 재배(栽培)하여 각각의 특성(特性)을 조사(調査), 기록(記錄)하였다. 수집자원(蒐集資源)의 종자(種子)는 정선(精選), 선별(選別) 한 후(後), 천립중(千粒重)을 측정(測定)하고 포장(包裝)하여 $4^{\circ}C$ 냉장고(冷藏庫)에 보관(保管)하였다. 수집(蒐集) 및 특성조사(特性調査)된 수집자원(蒐集資源)은 다음과 같다.

• 한국환경과학회지
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• 제20권4호
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• pp.527-540
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• 2011

The investigation was made about distribution and ecological characteristics of Rhododendron mucronulatum var. mucronulatum, R. dauricum and R. schlippenbachii on Mt. Changbai in China. The Ericaceae plants are known to be highly ornamental and easy to cultivate. The results of investigations are like followings. The plant species diversity was the highest in habitats of R. mucronulatum var. mucronulatum, followed by those of R. schlippenbachii and R. dauricum. R. mucronulatum var. mucronulatum community was found in the northwest exposure areas of 300m in altitude. Corylus heterophylla, Carex humilis var. nana, Atractylodes japonica species also grew in the R. mucronulatum var. mucronulatum community. R. dauricum communities were widely distributed in the west exposure areas of 1226-1860m above sea level. R. schlippenbachii populations were found in areas of under 300m above sea level, which has dry soil. But humidity of air was relatively high in the place. Artemisia japonica, Lespedeza bicolor and Dactylis glomerata were found around the habitats of R. schlippenbachii. Phalanx type of plants were dominant near the habitats of Ericaceae plants as 61%. Further, there appeared higher plant life totalling to 72 taxa comprising 54 species, 16 varieties and 2 forma with 31 families and 59 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 30.0%.