Browse > Article

Effects of Medium Supplements on Seed-Derived Callus Culture and Regeneration of Orchardgrass  

이상훈 (경상대학교 응용생명과학부)
이동기 (경상대학교 응용생명과학)
이병현 (경상대학교 응용생명과학부)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.49, no.3, 2004 , pp. 232-236 More about this Journal
Abstract
In order to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of a cultivar, 'Roughrider', as explant tissues. The optimal concentration of 2,4-D for the induction of embryogenic callus from mature seeds was 3 mg/L. Plant regeneration frequency was 36.3% when embryogenic calli were cultured on the regeneration medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Addition of 1 g/L casein hydrolysate and 300 mg/L L-proline improved frequencies of embryogenic callus induction and plant regeneration up to 57.3 and 60.7%, respectively. Supplementation of the media with 10 mga $\textrm{AgNO}_3$ and 40 mg/L cysteine enhanced frequencies of callus induction and plant regeneration. Efficient regeneration system established in this study will be useful for molecular breeding of orchardgrass through genetic transformation.
Keywords
Callus; forage crop; orchardgrass; regeneration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cho, M. J., W. Jiang, and P. G. Laumaux. 1998. Transformation of recalcitrant barley cultivars through improvement ofregenerability and decreased albinism. Plant Sci. 138 : 229-244   DOI   ScienceOn
2 Songstad, D., D. Duncan, and I. Witholm. 1988. Effect of 1-aminocyclopropane- 1-carboxylic acid, silver nitrate and norbornadiane on plant regeneration from maize callus cultures. Plant Cell Rep. 7 : 262-265   DOI   ScienceOn
3 Spangenberg, G., Z. Y. Wang, and Potrykus. 1998. Biotechnology in forage and turf grass improvement. In: Frankel et al (eds), Monographs on theoratical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, pp. 192-210
4 Van Wijk, A. J. P., J. G. Boonman, and W. Rumball. 1993. Achievements and prospectives in the breeding of forage grasses and legumes. In: Baker MJ (eds), Grasslands for our world, SIR, Wellington, pp. 116-120.
5 Chaudhury, A. and Q. Rongda. 2000. Somatic embryogenesis and plant regeneration of turf-type Bermuda grass: effect of 6-benzyladenine in callus induction medium. Plant Cell Tiss. Org. Cult. 60(2) : 113-120   DOI   ScienceOn
6 Vikrant and A. Rashid. 2002. Somatic embryogenesis from immature and mature embryos of a minor millet Paspalum scrobiculatum L. Plant Cell Tiss. Org. Cult. 69 : 71-77   DOI   ScienceOn
7 Bai, Y. and R. Qu. 2001. An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue (Festuca arundinacea Schreb.) cultivars. Grass Forage Sci. 55 : 326-330
8 Rao, A. M., S. K. Padma, and P. B. Kavi Kishor. 1995. Enhanced plant regeneration in grain and sweet sorghum by asparagine, proline andcefotaxime. Plant Cell Rep. 15: 72-75   DOI   ScienceOn
9 Trigiano, R. N., D. J. Gray, B. V. Conger, and J. K. McDaniel. 1989. Origin of direct somatic embryos from cultured leaf segments of Dactylis glomerata. Botanical Gazette 150 : 72-77.   DOI   ScienceOn
10 Vasilenko, A., J.K. McDaniel, and B. V.Conger 2000. Ultrastructural analyses of somatic embryo initiation, development and polarity establishment from mesophyll cells of Dactylis glomerata. In Vitro Cell Dev. BioI. Plant 36 : 51-56.   DOI   ScienceOn
11 Miller, D. A. 1984. Forage crops. McGraw-Hill, New York, pp. 396-409
12 Van Santen, E. and D. A. SIeper. 1996. Orchardgrass. In: Moser LE et al (eds), Cool-season forage grasses. Vol 34, ASA, CSSA, and SSSA, Madison WI, pp. 503-534
13 Van der Valk, P., F. Ruis, A. M. Tettelaar-Schrier, and C. M. van der Velde. 1995. Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass, the effect of benzyladenine. Plant Cell Tiss. Org. Cult. 40 : 101-103   DOI   ScienceOn
14 Conger, B. V, J. C. Hovanesian, R. Trigano, and D. J. Gray. 1989. Somatic embryo ontogeny insuspension cultures of orchardgrass. Crop Sci. 29 : 448-452.   DOI
15 McKersie, B. D. 1997. Improving forage production systems using biotechnolgy. In: McKersie BD and Brown DCW (eds), Biotechnology in Agriculture Series, No. 17, CAB International, Wallingford, pp. 3-21
16 Conger, B. V.,G. E. Hanning, D. J. Gray, and J. K. McDaniel. 1983. Direct embryogenesis from methophyll cells of orchardgrass. Science 221 : 850-851   DOI   PUBMED   ScienceOn
17 Horn, M. E., R. D. Shillito, B. V. Conger, and C. T. Harms. 1988. Transgenic plants of orchardgrass (Dactylis glomerata L.) from protoplasts. Plant Cell Rep. 7 : 469-472   DOI   ScienceOn
18 Hagio, T. 2002. Adeventitious shoot regeneration from immature embryos of sorghum. Plant Cell Tiss. Org. Cult. 68 : 65-72   DOI   ScienceOn
19 Chu, C. C., C. S. Wang, C. C. Sun, C. Hsu, K. C. Yin, C. Y. Chu, and F. Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18: 659-668
20 Eapan, S. and L. Goeorge. 1997. Plant regeneration from peduncle segments of oil seed Brassica species: Infulence of silver nitrate and silver thiosulfate. Plant Cell Tiss. Org. Cult. 51 : 229-232   DOI   ScienceOn
21 Zhong, H. and M. B. Sticklenn. 1991. Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustris Huds). Plant Cell Rep. 10 : 453-456
22 Murashige T and F. Skoog. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15 : 473-497   DOI
23 Griffin, J. D. and M. S. Dibble. 1995. High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.). Plant Cell Rep. 14: 721-724
24 Enriquez-Obregon, G. A., D. L. Prieto-Samsonov, G. A. de la Riva,and R. I. Vanquez-Padron. 1999. Agrobacterium-mediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment. Plant Cell Tiss. Org, Cult. 59 : 159-168   DOI   ScienceOn