• Journal of Microbiology and Biotechnology
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• 제19권7호
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• pp.685-689
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• 2009

We describe the expression of recombinant canstatin from stably transformed Bombyx mori BmS (BmS) cells. Recombinant canstatin was secreted into a culture medium with a molecular mass of approximately 29 kDa. Densitometric scanning showed that the secreted canstatin accounted for approximately 91% of the total canstatin production. Recombinant canstatin was also purified to homogeneity using a simple one-step Ni-NTA affinity fractionation. The identity of the purified protein was confirmed as human canstatin by nano-LC-MS/MS analysis. Purified recombinant canstatin inhibited human endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition ($ED_{50}$) for recombinant canstatin expressed in stably transformed BmS cells was approximately 0.64 ${\mu}g/ml$. A maximum production level of 11 mg/l recombinant canstatin was obtained in a T-flask culture of BmS cells after 6 days of incubation.

• 한국자기공명학회논문지
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• 제14권2호
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• pp.117-126
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• 2010

Hsp33 is a molecular chaperone achieving a holdase activity upon response to a dual stress by heat and oxidation. Despite several crystal structures available, the activation process is not clearly understood, because the structure inactive Hsp33 as its reduced, zinc-bound, monomeric form has not been solved yet. Thus, we initiated structural investigation of the reduced Hsp33 monomer by NMR. In this study, to overcome the high molecular weight (33 kDa), the protein was triply isotope-[$^{13}C$, $^{15}N$, $^2H$]-labeled and its inactive, monomeric state was ensured. 2D-[$^1H$, $^{15}N$]-TROSY and a series of triple resonance spectra could be successfully obtained on a high-field (900 MHz) NMR machine with a cryoprobe. However, under all of the different conditions tested, the number of resonances observed was significantly less than that expected from the amino acid sequence. Thus, a possible contribution of dynamic conformational exchange leading to a line broadening is suggested that might be important for activation process of Hsp33.

• 약학회지
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• 제54권4호
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• pp.316-321
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• 2010

Adefovir dipivoxil which was originally developed by Gilead Sciences has been used as treatments of HIV and HBV, especially a therapeutics for HBeAg positive and negative chronic patients. We developed highly efficient purification method using reverse phase column chromatography for mass production and a stable amorphous Adefovir dipivoxil using solid dispersion method. Reverse phase column chromatography led to highly pure product, more than 99.7% by HPLC and can be used for mass production compared with normal column chromatography. Solid dispersion method containing watersoluble polymer and Isomalt showed improved stability of amorphous Adefovir dipivoxil against heat and moisture.

• 한국환경과학회지
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• 제18권12호
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• pp.1399-1410
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• 2009

The study on community structure of benthic macroinvertebrates from wetland Woopo was conducted for the long-term ecological monitoring. The wetland Woopo is located in Changnyeong-Gun, Gyeongsangnam - Do of Korea. In 2006, we investigated the seasonal variation of benthic macroinvertebrates with quantitative and qualitative collecting methods. The collection was performed monthly at four surveying region, Woopo, Sajipo, Mokpo and Topyong stream. In this study, 6 classes, 16 orders, 48 families, 95 species were identified on group of benthic macroinvertebrates. The species diversity index and the species richness index were the highest in Topyung region and it was 3.222 and 10.216 respectively. The two species, Cercion calamorum and Cloeon dipterum were quantitatively collected for 9 months. The changes of body lengths of 50 individuals were recorded and the advanced growth on the two species was expected from present study.

• 대한기계학회논문집A
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• 제24권3호
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• pp.634-644
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• 2000

Fatigue crack growth and life is estimated by various fracture mechanical parameters but affected by load, material and environment. Fatigue character of component without surface notch cannot be e valuated by above-mentioned parameters due to microstructure of in-service material. Single fracture mechanical parameter or nondestructive parameter cannot predict fatigue damage in arbitrary boundary condition but multiple fracture mechanical parameters or nondestructive parameters can Fatigue crack growth modelling with three point representation scheme uses this merit but has limit on real-time monitoring. Therefore, this study shows fatigue damage model using backpropagatior. neural networks on the basis of X-ray half breadth ratio B/$B_o$ fractal dimension $D_f$ and fracture mechanical parameters can predict fatigue crack growth rate da/dN and cycle ratioN/$N_f$ at the same time within engineering estimated mean error(5%).

• 생약학회지
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• 제31권2호
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• pp.174-184
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• 2000

From our previous report, the water extract of Pulsatilla koreana root was found to have potent anti-inflammatory and analgesic actions in intravenous administration in animals. Among chloroform, ethyl acetate, butanol and water fractions which were obtained through successive fractionation of the extract, only the water fraction was found to have the antiinflammatory and analgesic actions. The fraction did not affect normal body temperature at the effective doses in mice and showed low acute toxicity of which $LD_{50}$ was less than 500 mg/kg i.v. in mice. It is interesting that its anti-inflammatory action might be attributed in part to inhibition of cyclooxygenase-1 and -2.

• Membrane and Water Treatment
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• 제11권1호
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• pp.25-29
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• 2020

The purpose of this study was to investigate the characteristics of lignin fractionated from waste wood (WW) using a two-step process of ethanol organosolv pretreatment followed by ultrafiltration with membranes of different molecular weight cut-offs (1, 5 and 20 kDa). The different permeates obtained were characterized by fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA) and gel permeation chromatography (GPC). The analysis by FT-IR and NMR of these lignins showed that the lignin core was successfully separated from WW. TGA curves confirmed that the thermal properties of lignin fractionated by ultrafiltration were almost identical to each other. The results from GPC confirmed that fractionating of lignin was achieved by ultrafiltration. For the membrane fractionation process, values of molecular weight decreased as the cut-offs used to obtain the fractions became smaller. As a result, fractionating lignin by a two-step process allowed separating different fractions of lignin of different molecular weights yielded high purity without interference from existing pollutants in WW. The two-step process offers the possibility of using fractionated WW as an untapped source of lignin.

• 한국수산과학회지
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• 제51권3호
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• pp.328-331
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• 2018

We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against nervous necrosis virus (NNV, RGNNV genotype). We established six hybridoma clones secreting MAbs against NNV antigen: 2B1, 2B11, 2C12, 13C1-1, 13C1-2 and 14D11. All six MAbs belonged to the IgG2a isotype with a kappa light chain and their reactivity recognized against the 41 kDa coat protein of NNV by Western blot analysis. The affinity constants of the six MAbs were measured by enzyme-linked immunosorbent assay (ELISA). All six MAbs reacted with two NNV isolates (SgNag05 and Gemunodo06), while no reactivity was observed with five know fish viruses, namely marine birnavirus, infectious pancreatic necrosis virus, viral hemorrhagic septicemia virus, hirame rhabdovirus, and infectious hematopoietic necrosis virus. Moreover, high ELISA optical density (OD) values (0.87-1.42) were observed in the brain tissues of NNV-infected sevenband grouper, while low OD values (less than 0.12) were recorded in the brain tissues of uninfected fish. These results suggest that these six MAbs are highly competent and useful for the detection of NNV with the RGNNV genotype.

• 한국기계가공학회지
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• 제18권12호
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• pp.1-6
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• 2019

Aluminum liners used in cylinders are hazardous for human health. In this study, we use a plastic PA liner inside cylinders to solve this problem. Plastic PA liners are widely used in the manufacturing industry in the production of food and beverage containers. We covered the aluminum boss with a plastic liner material and wound the composite fibers over the liner material. To reinforce the dome area, we used low strength / high elongation plastic liner. To predict the performance of the developed product, we conducted structural analyses utilizing the 3D laminated solid element. We verified the soundness of the product by testing the prototype.

• Ocean and Polar Research
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• 제25권3호
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• pp.249-256
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• 2003

An immunological method was developed in this study to quantify reproductive output of the female butter clam, Saxidomus purpuratus. A clam egg-specific polyclonal antibody was developed using the purified butter clam egg as an antigen. An indirect Enzyme-Linked Immunosorbent Assay (ELISA) was used in quantitative measurement of the eggs. Size of the butter clam eggs ranged from $70.81{\pm}7.52{\mu}m$ in histology or $88.56{\pm}11.31{\mu}m$ in intact eggs. The predominant egg constituent was protein (37.44%), followed by lipids (11.40%) and carbohydrates (9.68%). The SDS-PAGE showed that the egg proteins are composed of several peptides of molecular weights consisting of 247, 200, 99, 91, 54 and 47 kDa. ELISA indicated that the clams collected from Geoje Island in May 2002 produced 8.2 to 26.8% of their body weight as eggs or 9,307,309 to 31,156,333 with a mean of 16,931,893 eggs per individual clam. The results of this study thus suggest that indirect ELISA using rabbit anti-clam egg IgG as a primary antibody is a rapid, affordable and sensitive method to assess reproductive output of 5. purpuratus and possibly other bivalves using a small amount of eggs.