• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.246.2-246
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• 1995

No Abstract, See Full Text

• 한국식품과학회지
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• 제31권2호
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• pp.540-546
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• 1999

참치 백자의 항균제호서의 사용 가능성 검토를 위하여 참치 백자의 특성 및 항균력 실험을 하였다. 아미노산 분석결과 arginine 함량은 약 46%로 연어 백자로부터 추출한 standard protamine (SP, Asama 화성, Japan)에 비해 약 10%가 낮았으며, proline및 glycine등 아미노산 조성에서도 큰 차이가 있었다. 주된 단백질의 평균 분자량은 SP가 11,300 Da과 2,600 Da이었으며, TP는 13,400 Da이었다. 그리고 SP와 TP의 항균활성 비교 실험에서 TP는 SP보다 약한 것으로 나타났다. 항균활성을 높이기 위해 $37^{\circ}C$에서 효소처리한 pepsine hydrolyzed protamine (PHP)과 trypsine hydrolyzed protamine (THP)의 주된 단백질의 평균 분자량은 PHP의 경우 11,300 Da과 3,900 Da으로 확인되었고, THP의 분자량은 2,500 Da 이하로 나타났다. PHP의 항균활성을 확인한 결과 TP보다 높은 항균력을 보였으며, 주된 단백질의 평균 분자량이 비슷한 SP와 유사하게 항균활성이 향상됨을 알 수 있었다. 그러나 gram (+)균인 Stap. aureus, L. helveticus, L. plantarum 등에서는 PHP가 SP보다 강한 항균활성을 보여 주었다. 그리고 분자량이 2,500 Da 이하인 THP의 경우 항균활성이 없는 것으로 밝혀졌다. 이와같이 PHP의 항균활성이 향상된 것은 protamine의 arginine의 함량이 증가하지 않더라도 주된 단백질의 평균 분자량이 변화되었기 때문으로 생각된다. 따라서 참치 백자로부터 추출한 TP를 pepsin처리했을 때 SP와 유사한 수준으로 항균활성 향상되는 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.561-566
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• 2014

The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by $Ni^{2+}$ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

• 한국해양학회지:바다
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• 제20권3호
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• pp.119-130
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• 2015

동해 해양자료동화시스템(DA-ESROM; Kim et al., 2009)을 이용하여 Argo 관측망이 해양 분석장에 미치는 영향에 대해 살펴보았다. 본 연구에서는 2009년을 연구기간으로 하여 수온 프로파일, 해수면 온도, 그리고 해수면 고도 자료를 동화하여 분석장을 생산하고(Exp. AllDa), 이를 Argo 수온 자료를 제외한 실험(Exp. NoArgo) 결과와 비교하였다. 동해 수온 프로파일 관측자료와 두 실험결과와의 평균 제곱근 오차(Root Mean Square Error; RMSE)를 살펴본 결과, Exp. AllDa의 결과에서 Exp. NoArgo에 비해 표층 이하부터 전반적으로 낮은 RMSE가 나타났고, 특히 수심 약 100 m 부근에서 약 $0.5^{\circ}C$의 RMSE 차이(Exp. AllDa - Exp. NoArgo)를 보이는 등 아표층 부근에서 Argo 수온 자료동화의 영향이 큰 결과를 보였다. 자료동화 과정에 독립적인 표류부이 관측자료와의 비교를 통해, Argo 수온 자료의 동화로 표층해류 정확도가 전반적으로 개선되는 것을 확인하였고, 특히 동해 중남부에서 상대적으로 장기 표류한 부이의 궤적을 따라 RMSE가 약 2.0~6.0 cm/s 정도 낮아졌다. 반면, 표층수온에 대해서는 Argo 수온자료의 동화효과는 약한 것으로 나타났고, 매일 동화되는 해수면 온도 자료의 영향이 지배적인 것으로 판단된다. 또한, 동해 해양자료동화시스템(DA-ESROM)은 일주일 간격으로 해수면 고도자료를 동화하지만, Argo 수온자료가 동화되지 않으면서 나타나는 해수면 고도 변화를 완전히 보정하지 못하는 것으로 나타났다. 실험결과, Argo 수온자료의 동화는 특히 야마토 분지 남서쪽의 시계방향 순환 등 동해 중남부 해역에서의 해수면 고도 재현성을 향상시키는데 큰 영향을 미치는 것으로 나타났다.

• Fisheries and Aquatic Sciences
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• 제15권4호
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• pp.293-297
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• 2012

${\gamma}$-Aminobutyric acid-enriched sea tangle extract was obtained from the fermentation of Lactobacillus brevis BJ-20. The fermented sea tangle extract (FST) was separated into three fractions by molecular weight: FST I (greater than 10 kDa), FST II (1-10 kDa), and FST III (less than 1 kDa). The anti-inflammatory characteristics of the FST fractions were investigated by measuring the production of nitric oxide (NO) and the expression levels of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced mouse macrophage (RAW 264.7) cells. Both NO production and iNOS expression levels were significantly inhibited by FST treatments in a dose-dependent manner. FST III was the most effective inhibitor of processes. This demonstrates that the effect of FST on LPS-induced inflammation might be closely correlated with the inhibition of inflammatory cytokine expression.

• 동의신경정신과학회지
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• 제17권3호
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• pp.11-19
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• 2006

Objective : This study was to investigate the utility and relation of dementia rating test(K-DRS and IADL, NPI-Q(symptom), NPI-Q(suffering), CCDR, SDS Method : For this study, we carried out dementia assessment examination of 34 patients with memory disturbance who have come to Cheongju oriental hospital of Daejeon university from April 2005 to February 2006. This study classified the patients as none-dementia(ND), questionable dementia(QD), and dementia(DA) groups and analyzed the result of examination. Results: 1. K-DRS and SDS, K-DRS and SDS, NPI-Q(symptom) and NPI-Q(suffering), NPI-Q(symptom) and CCDR showed clear correlations statistically each other. 2. K-DRS scores showed the significant differences from that of ND and the other groups ; attention and conceptualization showed the significant differences between ND and DA, management and memorization showed the significant differences between DA and the other groups. 3. IADL scores showed the significant differences from that of DA and the other groups, NPI-Q(symptom) scores showed the significant differences between QD and DA, NPI-Q(suffering) scores showed no differences among all groups. 4. CCDR scores showed the significant differences from that of DA and the other groups, SDS scores showed the significant differences between ND and DA 5. MMSE- K and K-DRS showed strong correlations statistically each other. Conclusion : The study results suggest that dementia rating tests is useful to esteem the dementia and the dementia rating tests have strong corelations each other. We use the above mentioned tests for correct diagnosis.

• Biomolecules & Therapeutics
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• 제12권3호
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• pp.157-164
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• 2004

DA-3711 is a novel anti-wrinkle agent containing growth factors derived from culture medium of artificial human skin. Photoprotective effect by DA-3711 against chronic UVB (ultraviolet B)-induced skin damage was investigated in hairless mice model. Methods: After hairless mice were irradiated to induce photodamage for 8 weeks with UVB, grouped mice were treated topically once a day with lotion base, DA-3711 (30% or l5%), Cylasphere retinol$^{\circed{R}}$ (2500 I.U.), NouriCel$^{\circed{R}}$ along with concomitant exposure to UVB for further 8 weeks. Then mice were sacrificed to assess photodamage-protective effect by replica analysis, biochemistry and histology. DA-3711 of 30% lotion significantly reduced UVB radiation-induced wrinkling, histological alterations and increased collagen contents. Whereas DA-3711 of l5% lotion and NouriCel$^{\circed{R}}$ treatment showed a partial protective effect on skin wrinkle, epidermal and dermal thickness, and collagen content, Cylasphere retinol$^{\circed{R}}$ showed no protective effects. These results demonstrate that topical application of DA-3711 can alleviate UVB-induced photodamage and potentially be used for reduction of UVB-induced photodamage.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.628-634
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• 2002

A microfilament-based motility in Toxoplasma gondii (T. gondii) Is involved in host cell invasion, yet the exact mechanism has not yet been determined. Accordingly, the current study examined the localization of actin and tubulin in T gondii using immunofluorescent (IF) and immunogold staining for electron microscopy. Indirect immunofluorescence (IF) staining using anti-actin and anti-tubulin monoclonal antibodies (mAbs) revealed localization of fluorescence on the entire surface of the tachyzoites. The actin in T. gondii was observed by immunogold staining, and the gold particles were seen on the surface, especially at the anterior end and in the cytoplasm of the parasite. However, there were no gold particles in the nucleus, rhoptries, and dense granules. The tubulin in T gondii was located on the surface and in the cytoplasm of the tachyzoites in the extracellular parasite, compared with anterior part of tachyzoites in the intracellular parasite. The antigens of T gondii recognized by anti-actin mAb were 107 kDa, 50 kDa, 48 kDa, and 40 kDa proteins, while those recognized by anti-tubulin mAb were 56 kDa, 52 kDa, and 34 kDa proteins. Tachyzoites of T gondii pretreated with the actin inhibitor, cytochalasin D (20 $\mu\textrm{g}$/ml), and tubulin inhibitor, colchicine (2$\times$10$\^$-6/ M), for 30 min at 37$\^{C}$ were used to infect the isolated mouse macrophages (tachyzo ites:macrophage=2:1). Pretreatment with the inhibitors resulted in lower multiplication of tachyzoites within the macrophages than in the untreated group 18 h post infection (p<0.05). Therefore, the present results suggest that actin and tubulin appear to be involved in the invasion of and multiplication in host cells.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.474-482
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• 2004

The $\alpha$-L-arabinofuranosidase (Arfase) gene of Bacillus stearothermophilus No. 236 was cloned and sequenced. The ORF of the gene, designated arfA, encoded a 507 -residue polypeptide with calculated molecular mass of 57 kDa. The Arfase produced by a recombinant Escherichia coli strain containing the arfA gene was purified to apparent homogeneity and characterized. The molecular mass of the Arfase determined by SDS-PAGE was 60 kDa. However, according to gel filtration, it was estimated to be approximately 190 kDa. These results indicated that the functional form of the Arfase is trimeric. The optimal pH and temperature for the enzyme activity were pH 6.5 and $55^{\circ}C$, respectively. The half-life of the enzyme at $60^{\circ}C$ was about 6 h. Kinetic experiments at $45^{\circ}C$ with pNPM (p-nitrophenyl $\alpha$-L-arabinofuranoside) as a substrate gave the $K_m and V_{max}$ values of 1.19 mM and 26.1 U/ mg, respectively. When the enzyme was combined with Bacillus stearothermophilus No. 236 endoxylanase and $\beta$-xylosidase, it hydrolyzed arabinoxylan into L-arabinose and xylose more efficiently than Arfase alone. This synergistic effect suggested that the complete hydrolysis of xylan with large amounts of arabinose side chains required Arfase as well as endoxylanase and $\beta$-xylosidase.

• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.2042-2045
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• 2007

The effect of chitosan oligosaccharide (COS, 1 kDa${\gamma}$, 10 ng/ml IL-$1{\alpha}$, and 25 ng/ml TNF-${\alpha}$) in HT-29 cells. Inducible nitric oxide synthase (iNOS) expression induced by these cytokines was inhibited by COS. COS pretreatment inhibited the invasiveness of cytokines-treated HT-29 cells through Matrigel-coated membrane in a dose-dependent manner. COS also inhibited cytokines-induced matrix metalloproteinase (MMP)-2 activity. This study shows that proinflammatory cytokines induce NO production, iNOS expression, and invasiveness of human colorectal adenocarcinoma HT-29 cells. COS pretreatment inhibited cytokines-mediated NO production, iNOS expression, and invasiveness of HT-29 cells. These results provide sufficient information for the further development of COS as an antitumor metastatic agent for the treatment of colon cancer.