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http://dx.doi.org/10.5657/FAS.2012.0293

Effects of γ-Aminobutyric Acid (GABA)-Enriched Sea Tangle Laminaria japonica Extract on Lipopolysaccharide-Induced Inflammation in Mouse Macrophage (RAW 264.7) Cells  

Choi, Ji-Il (Department of Food Science and Technology, Pukyong National University)
Yun, In-Hye (Department of Food Science and Technology, Pukyong National University)
Jung, Yeounjoong (Department of Food Science and Technology, Pukyong National University)
Lee, Eun-Hye (Department of Food Science and Technology, Pukyong National University)
Nam, Taek-Jeong (Department of Food Science and Nutrition, Pukyong National University)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.15, no.4, 2012 , pp. 293-297 More about this Journal
Abstract
${\gamma}$-Aminobutyric acid-enriched sea tangle extract was obtained from the fermentation of Lactobacillus brevis BJ-20. The fermented sea tangle extract (FST) was separated into three fractions by molecular weight: FST I (greater than 10 kDa), FST II (1-10 kDa), and FST III (less than 1 kDa). The anti-inflammatory characteristics of the FST fractions were investigated by measuring the production of nitric oxide (NO) and the expression levels of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced mouse macrophage (RAW 264.7) cells. Both NO production and iNOS expression levels were significantly inhibited by FST treatments in a dose-dependent manner. FST III was the most effective inhibitor of processes. This demonstrates that the effect of FST on LPS-induced inflammation might be closely correlated with the inhibition of inflammatory cytokine expression.
Keywords
Sea tangle; Laminaria japonica; Anti-inflammatory activity; ${\gamma}$-Aminobutyric acid; RAW 264.7 cells;
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Times Cited By KSCI : 7  (Citation Analysis)
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