• Title/Summary/Keyword: DWI

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Implementation of Regional Cerebral Blood Volume Map Using Perfusion Magnetic Resonance Image Process Algorithm (관류자기공명 영상처리 알고리즘을 이용한 대뇌 혈류량 맵의 구현)

  • Park Byung-Rae
    • The Journal of the Korea Contents Association
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    • v.5 no.5
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    • pp.296-304
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    • 2005
  • Quantitative analysis compare to dynamic characteristic change of the regional cerebral blood volume(rCBV) after development of cerebral fat embolism in cats using perfusion magnetic resonance(MR) Imaging. Twenty cats were used. Linoleic acid (n=11) were injected into the internal carotid artery using microcatheter through the transfemoral approach. Polyvinyl alcohol (Ivalon) (n=9) was injected as a control group. Perfusion MR images were obtained at 30 minutes and 2 hours after embolization, based on T2 and diffusion-weighted images. The data was time-to-signal intensity curve and ${\Delta}R_2^*$ curve were obtained continuously with the aid of home-maid image process algorithm and IDL(interactive data Banguage, USA) softwares. The ratios of rCBV increased significantly at 2 hours compared with those of 30 minutes (P<0.005). In conclusion, cerebral blood flow decreased in cerebral fat embolism immediately after embolization and recovered remarkably in time course. It is thought that clinically informations to dynamic characteristic change of the cerebral hemodynamics to the early finding in cerebral infarction by diffusion weighted imaging(DWI) and perfusion weighted imaging(PWI).

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Strength Map of Presidential Candidates 2019 in Indonesia Based on a NodeXL Analysis of Big Data from Twitter

  • Suratnoaji, Catur;Arianto, Irwan Dwi;Sumardjijati, Sumardjijati
    • Asian Journal for Public Opinion Research
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    • v.6 no.1
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    • pp.31-38
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    • 2018
  • Leading up to the 2019 presidential election in Indonesia, campaigns have emerged through social media, particularly Twitter, using various hashtags, such as #2019GantiPresiden (2019 Change President) and #TetapJokowi (Always Jokowi). This paper tries to understand the presidential candidates' power map in forming opinions and influencing voter behavior by analyzing Twitter from August 6, 2018 to September 15, 2018, just before the beginning of the official campaign period, by searching for the keyword "pemilihan presiden RI Tahun 2019" (RI presidential election in 2019). According to our NodeXL's analysis, there were 1,650 active Twitter users talking about the 2019 presidential election. The 1,650 Twitter users have formed a communication network of 46,750 relationships formed from messages in the form of tweets, comments, and retweets. Our analysis found that those mentioning "pilihan presiden 2019" form large communication networks around four clusters: one for each of the two candidates (Jokowi and Prabowo) and two for opinion leaders who are undecided about the election (Gus Mus and Mas Piyu). GusMus is a religious leader, as an official of the PBNU Rais Syuriah (an Islamic organization) and has a large following both on and off Twitter. "MasPiyu" is an unidentified Twitter user; he only has a large following on Twitter, but does not have support offline.

A Study on Comparative Analysis of Diffusion Weighted Image Examination before and after Contrast Injection (조영제 사용 전 후 확산강조영상 검사의 비교 분석에 대한 연구)

  • Goo, Eun-Hoe
    • Korean Journal of Digital Imaging in Medicine
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    • v.11 no.2
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    • pp.51-57
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    • 2009
  • The purpose of this study would evaluate if having clinical effects on diffusion image with quantitative analysis through ADC values of brain's normal tissue and lesions before and after contrast injections using a 3.0T. From November in 2007 until December in 2008, a total of 32 patient was performed on 3.0T(Signa Excite, GE Medical System, USA) with the normal or lesions in the patient who requests diffusion weighted image with 8channel head coil. The pulse sequence was used with spin echo EPI(TR: 10000msec, TE: 72.2 msec, Matrix: 128*128, FOV: 240 mm, NEX: 1, diffusion direction: 3, b-value: 1000). Measurement results of ADC values on lesions, CSF, white matter, gray matter, lesions after contrast injection were measured less 75% than before contrast injection, infarction: 100%, CSF: 78%(high), white matter: 71.4%(low), gray matter: 50%(high, low). The results of paired t-test on the deference of ADC values which statically is significant in three(lesions, CSF, white matter)regions except for white matter(p<0.05). Quantitative analysis of lesions, CSF, white matter, gray matter have difference on all regions. ADC values were low in lesions and white matter, normal CSF after contrast injection commonly is high than before contrast injection, ADC values which white matter were high and low (50:50) after contrast injection. 3.0T diffusion weighted image clinically supposed that performing DWI examination after contrast injection was not desirable because of having effects on brain tissue.

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Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

  • Jang, Hyun A;Utomo, Setyo Dwi;Kwon, Suk Yoon;Ha, Sun-Hwa;Xing-guo, Ye;Choi, Pil Son
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.341-346
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    • 2016
  • The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNA-PCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hybridization. This study successfully demonstrated the production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.

Effect of Supplementation of Zinc, Glutamine, Fiber, and Prebiotics in Presumed Healthy Indonesian Children Aged 1-3 Years

  • Kadim, Muzal;Hegar, Badriul;Bardosono, Saptawati;Timan, Ina S;Gunardi, Hartono;Prasetyo, Dwi;Firmansyah, Agus;Vandenplas, Yvan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.4
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    • pp.388-396
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    • 2020
  • Purpose: Impaired intestinal mucosal integrity may affect the gastrointestinal function, especially in relation to nutrition, absorption, and barrier function. The purpose of this study was to measure the prevalence of impaired intestinal mucosal integrity in presumed healthy children aged 1-3 years and assess the effects of zinc, glutamine, fiber, and prebiotic supplementation in them. Methods: A cross-sectional study was conducted in 200 children aged 1-3 years in Pasar Minggu, South Jakarta, Indonesia. A randomized double-blind parallel group method clinical trial was then performed to assess the effects of zinc, glutamine, fiber, and prebiotic supplementation. Results: Elevated calprotectin was found in 91/200 subjects (45.5%) at the onset of the study. After 10 months, 144 subjects completed the study: 72 subjects received the trial formula, whereas the other 72 received the standard formula. A transitory decrease in fecal calprotectin (FC) was observed after 6 months in the subgroup with normal FC levels, who were fed the test formula (p=0.012). Conclusion: The prevalence of impaired intestinal mucosal integrity in this group of Indonesian children aged 1-3 years was high. Supplementation with zinc, glutamine, fiber, and prebiotics during 6 months reduced FC only in those who had low levels at baseline but not in those with impaired integrity.

A Change of Apparent Diffusion Coefficient in Diffusion Weighted Imaging Applied with Rectangular FOV Technique (확산강조영상 검사 시 rectangular FOV 적용에 따른 ADC 값의 변화)

  • Na, Sa-Ra;Choi, Kwan-Woo;Koo, No-Hyun;Yoo, Beong-Gyu;Son, Soon-Yong
    • Journal of the Korean Society of Radiology
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    • v.10 no.7
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    • pp.545-550
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    • 2016
  • The purpose of this study is to improve the mapping between functional image and conventional image by using rectangular FOV technique. Diffusion weighted imaging which is widely used for stroke was acquired by reducing the FOV and compared each Apparent Diffusion Coefficient(ADC). As a result, there is no significant difference of each ADC value in one-way-anova analysis and post-hoc analysis. Thus, Mismatching problem may be improved by matching the FOV with rectangular FOV technique because there is no difference in ADC values.

The development of herbicide-resistant maize: stable Agrobacterium-mediated transformation of maize using explants of type II embryogenic calli

  • Kim, Hyun A.;Utomo, Setyo Dwi;Kwon, Suk Yoon;Min, Sung Ran;Kim, Jin Seog;Yoo, Han Sang;Choi, Pil Son
    • Plant Biotechnology Reports
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    • v.3 no.4
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    • pp.277-283
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    • 2009
  • One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

Fiber Morphology and Physical Characteristics of Gigantochloa atter at Three Different Ages and Heights of Culms for Better Utilization

  • Marsoem, Sri Nugroho;Setiaji, Fajar;Kim, Nam-Hum;Sulistyo, Joko;Irawati, Denny;Nugroho, Widyanto Dwi;Pertiwi, Yus Andini Bekti
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.2
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    • pp.145-155
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    • 2015
  • Fiber morphology and basic characteristics of Legi bamboos (Gigantochloa atter) growing on Yogyakarta were studied considering their age and height positions in the culms. Culms of 4, 16, and 40 months were harvested, and their total lengths were measured. The length, diameter, and wall thickness of each internode were measured. All the sample culms were divided into three different parts along the height, and their fiber dimension and physical properties were observed. The data obtained were analyzed by analysis of variance. The results showed that the culms had a diameter of 5.8 to 10.8 cm. The lowest internodes always showed the shortest length and the thickest wall. The culms had an average fiber length of 2.41 mm and Runkel ratio of 0.61. Fiber length was affected by the height, while fiber diameter, lumen diameter, and fiber wall thickness were affected significantly by the age of the culms. The culms had high green moisture content (GMC) of 157.89%, and basic density (BD) of $456.67kg.m^{-3}$, a total longitudinal shrinkage of 0.35%, and relatively low R/T shrinkage ratio. The interactions between age and height were affected GMC and BD.

Conditioned medium of E17 rat brain cells induced differentiation of primary colony of mice blastocyst into neuron-like cells

  • Budiariati, Vista;Rinendyaputri, Ratih;Noviantari, Ariyani;Haq, Noer Muhammad Dliyaul;Budiono, Dwi;Pristihadi, Diah Nugrahani;Juliandi, Berry;Fahrudin, Mokhamad;Boediono, Arief
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.86.1-86.13
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    • 2021
  • Background: Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties. Objectives: This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties. Methods: The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium. Results: The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium. Conclusions: Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.

Determinants of Accessibility to Fintech Lending: A Case Study of Micro and Small Enterprises (MSEs) in Indonesia

  • SAPTIA, Yeni;NUGROHO, Agus Eko;SOEKARNI, Muhammad;ERMAWATI, Tuti;SYAMSULBAHRI, Darwin;ASTUTY, Ernany Dwi;SUARDI, Ikval;YULIANA, Retno Rizki Dini
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.10
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    • pp.129-138
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    • 2021
  • Several studies have revealed that information on borrower characteristics plays an important factor in approving their credit requests. Though the extent to which such characteritics are also applicable to the case of fintech lending remain uncertain. The aim of this study is, thus, to investigate the determinant factors that influence MSEs in obtaining credit through fintech lending. Here, we emphasize virtual trust in fintech lending encompasing the dimension of social network, economic attributes, and risk perception based on several indicators that are used as proxies. Primary data used in the study was gathered from an online survey to the respondents of MSEs in Java. The result of the study indicates that determinants of MSEs in obtaining credit from lender through fintech lending are statistically influenced by internet usage activities, borrowing history, loan utilization, annuity payment system, completeness of credit requirement documents and compatibility of loan size with the business need. These factors have a significant effect on credit approval because they can generate virtual trust of fintech lender to MSEs as potential borrowers. It concludes that the probability of obtaining fintech loans in accordance with their expectations are influenced by the dimensions of social network, economic attributes and risk perception.