• 약학회지
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• 제47권6호
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• pp.365-368
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• 2003

The bactericidal activities of DW286, a new fluoroquinolone were investigated by comparing the minimal bactericidal concentrations (MBCs) and the time-kill curve of it against some Gram-positive and Gram-negative bacterial strains. The MBCs of DW286 for the strains tested were either equal to or two-fold higher than the MICs, as were observed for the other fluoroquinolones. And DW286 exhibited rapid killing curves against the strains. Accordingly, it could be said that DW286 has bactericidal activity comparable to other fluoroquinolones.

• 약학회지
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• 제56권5호
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• pp.304-308
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• 2012

In vitro antibacterial activity of DW286 was tested against recently collected clinical isolates of Gram-positive strains by the two fold agar dilution method. In vivo activity of DW286 was also determined against systemic infections in mice caused by Staphylococcus aureus. DW286 showed 16~64-fold more potent in vitro activities than ciprofloxacin against Gram-positive bacteria. Against systemic infection model caused by two S. aureus strains, one being methicillin-susceptible and the other methicillin-resistant, DW286 ($ED_{50}s$, 0.16 mg/kg and 4.36 mg/kg, respectively) was more potent than gemifloxacin (1.37 mg/kg, 26.58 mg/kg, respectively).

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.70.2-71
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• 2003

Quinolone resistance in Streptococcus pneumoniae is related to mutations in the DNA gyrase and topoisomerase IV genes. DW-286a displayed potent activity against S. pneumoniae C9211 (MIC, 0.015 ${\mu}$g/ml) compared with gemifloxacin (MIC, 0.06 ${\mu}$g/ml). This study was performed to analyze the ability of DW-286a to cause resistance development in S. pneumoniae and to establish whether DNA gyrase or topoisomerase IV is primary target. DW-286a resistant mutants of S. pneumoniae C9211 were generated by stepwise selection at increasing drug concentration. (omitted)

• 약학회지
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• 제52권3호
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• pp.219-224
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• 2008

Clinically isolated methicillin-resistant Staphylococcus aureus strains were exposed to subinhibitory concentration of DW286, DW-224a, gemifloxacin, trovafloxacin, sparfloxacin and ciprofloxacin during 26- to 39-days period. Subculturing led to resistance development, and most of the selected mutants were above susceptible breakpoints. Selected mutants had broad cross resistance to other quinolone antibiotics and only one mutant was completely susceptible to all fluoroquinolones. Twenty five among 42 mutants revealed mutations on DNA gyrase and topoisomerase IV by sequencing. Also 16 mutants had fluoroquinolones MICs that were 4-32 times lower in the presence of reserpine. In conclusion, alterations in DNA gyrase or topoisomerase IV and action of efflux pumping out system are the resistance mechanisms of DW-224a.

• 한국작물학회지
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• 제32권3호
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• pp.277-286
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• 1987

인삼종자의 휴비 및 발아기구의 해명을 위한 기초자료를 얻고자 종자의 후숙과정중 ABA의 함양변화를 조사하여 미숙배의 생장 및 발아와의 관계를 검토하였다. 1. 홍숙과에 있어서 ABA 함량은 과육이 종자나 내과피보다 현저하게 높았으며 이들 분포는 수분분포와 비슷하였다. 2. 개갑처리과정중 ABA 함량은 종자 및 내과피에서 모두 점차 감소되었는데 처리 90 일후 (개갑률 :96 %)에 종자에서는 90 pmol/gDW로 69 %가, 내과피에 서는 41 pmol/gDW로 80 %가 각각 감소되었다. 전 ABA에 대한 free 능의 비율은 종자에서는 계속 감소하였으나 내과피에서는 종자보다 높은 채로 거의 변화가 없었다. ABA 함량 감소와 미숙배생장과의 상관에서는 종자에서 유의성(r=-0.9737)이 크게 나타났다. 3. 개갑후 종자의 ABA함량은 4$^{\circ}C$와 15$^{\circ}C$에서 모두 점차 감소되었는데 처리 90 일후에 4$^{\circ}C$에서는 28 pmol/gDW로 69%가, 15$^{\circ}C$에서는 46pmo1/gDW로 49%가 각각 감소되었다. 전 ABA에 대한 free 능의 비율은 4$^{\circ}C$에서는 점차 증가되었으나 15$^{\circ}C$에서는 거의 변화가 없었다. ABA 함량 감소와 발아시에서는 거의 변화가 없었다. ABA함량 감소와 발아시와의 상관에서는 4$^{\circ}C$에서만 유의성(r=0.9852)이 있었으며 ABA 함량 감소와 1일 평균 발아율과의 상관에서는 4 $^{\circ}C$에서 부상관(r=-8548), 15$^{\circ}C$에서는 정상관 (r =0.9737)이 있었다. 4. 이상의 결과로 미루어 보아 개갑과정에서의 ABA의 함랸변화는 미숙배의 생장과 밀접한 관계가 있으며 개갑후 저온처리 과정에서의 발아능력(생리적휴면타파정도)은 ABA의 함량감소와는 직접적인 관계가 없는 것 같다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.77.1-77.1
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• 2003

Prolongation of the QT interval may result in a potentially dangerous arrhythmia. The most commonly proposed mechanism for QT interval prolongation(LQT) by pharmaceuticals is inhibition of the rapid delayed rectifier potassium channel (I$\sub$Kr). The LQT potency of pharmaceuticals can be effectively evaluated by examining the effect on human ether-a go-go-related gene (hERG) channels expressed in CHO cells, known to be equal to I$\sub$kr/. We have transfected JERG into CHO cell lines transiently to express high levels of functional hERG channels. (omitted)

• KSBB Journal
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• 제28권5호
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• pp.282-286
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• 2013

The seaweed, Gelidium amansii, was fermented to produce bioethanol. Optimal pretreatment condition was determined as 94 mM $H_2SO_4$ and 8% (w/v) seaweed slurry at $121^{\circ}C$ for 60 min. The mono sugars of 40.4 g/L with 67% of conversion from total carbohydrate of 60.6 g/L with 80 g dw/L G. amansii slurry were obtained by thermal acid hydrolysis pretreatment and enzymatic saccharification. G. amansii hydrolysate was used as the substrate for ethanol production by Kluyveromyces marxianus KCTC 7150 and Candida tropicalis KCTC 7212 using 5L fermentor. The ethanol productions by K. marxianus KCTC 7150 and C. tropicalis KCTC 7212 were 17.8 g/L with $Y_{EtOH}$ of 0.48 at 120 h and 19.3 g/L with $Y_{EtOH}$ of 0.50 at 120 h, respectively.

• 한국환경농학회지
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• 제34권3호
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• pp.186-191
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• 2015

BACKGROUND: Carotenoids which are a major source of vitamin A are contributed to have great potential role in anti-carcinogenic effects and eyesight. Carotenoids which can not synthesize in human body are required for food supply. The objectives of this study are to investigate compositions and contents of pumpkin (Cucurbita spp.) germplasms based on their pulp color. METHODS AND RESULTS: Carotenoids were extracted with 0.2% ascorbic acid in ethanol and saponified with 80% potassium hydroxide. Insoluble compounds were extracted into hexane. A total of nine carotenoids (three xanthophylls and six carotenes) were identified from pumpkin germplasms using HPLC equipped with photodiode array detector (450 nm). Especially, lutein and ${\beta}$-carotenes were major compound in germplasms. Among isomers of ${\beta}$-carotene, all-trans-${\beta}$-carotene (16-27% of total carotenoids) was predominant compositions. The mean of total carotenoid contents was showed as brown (286.1 mg/100 g dw) > dark green (217.0) > orange (153.4) > primrose (85.8) > dark yellow (80.3). On the basis of carotenoid information, PLS-DA score plots showed different patterns by cluster in pumpkin germplasms. It was considered that these differences of phenotype were relative closely to genotype. CONCLUSION: This study indicated that dark color of pumpkin pulp was presented in high-level of biological pigments. It may contribute to develop potentially beneficial functional food ingredients.