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Fractionation and Potential Mobility of Heavy Metals in Tailings and Paddy Soils near Abandoned Metalliferous Mines (폐광산 광미와 논토양의 중금속 분획화 및 잠재적인 이동도)

  • Jung, Goo-Bok;Lee, Jong-Sik;Kim, Won-Il;Kim, Jin-Ho;Shin, Joung-Du;Yun, Sun-Gang
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.5
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    • pp.259-268
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    • 2005
  • Most of the tailings have been left without any management in their mines and have become the main source of serious environmental problems in nearby groundwater, stream and cultivated lands. To compare fractionation and potential mobility of heavy metals in tailings and paddy soils near abandoned 10-metalliferous mines in Korea, the distribution and chemical fractions of heavy metal and their mobility in relation to chemical compositions were investigated. The pollution index of heavy metal in mine tailing calculated with the permissible levels were in the order Cheongyang>Dogok>Beutdeun>Baegwoul mine, which were considered sufficient to raise environmental problems. The rates of 0.1M-HCl extractable Cd, Cu, Pb, Zn, and Ni to total content in paddy soils were 49.1, 50.7, 26.8, 18.4 and 2.9%, respectively, and their rates of heavy metals in paddy soils were higher than that of mine tailing. Dominant chemical forms of heavy metals in tailings were sulfide and residual form (63-91%), specially, the exchangeable portion of Cd (21%) was relatively higher than that of other metals in paddy soils. The mobility factor of heavy metals in tailings and paddy soils was in the order Cd>Zn>Cu>Pb, and the mobility factor in tailing varied considerably among the mines. The potential mobility of heavy metals in tailings showed significant positive correlation with water-soluble $Al^{3+}$ and $Fe^{3+}$ contents, while in paddy soils, it correlated negatively with soil pH values.

Predicting N2O Emission from Upland Cultivated with Pepper through Related Soil Parameters (온실가스 배출 파라메타를 이용한 고추밭 토양의 N2O 배출 예측)

  • Kim, Gun-Yeob;Song, Beom-Heon;Hyun, Byung-Keun;Shim, Kyo-Moon;Lee, Jeong-Taek;Lee, Jong-Sik;Kim, Won-Il;Shin, Joung-Du
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.5
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    • pp.253-258
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    • 2006
  • An empirical model of nitrous oxide emission from agricultural soil has been applied. It is based on the relationship between $N_2O$ and three soil parameters, soil mineral N(ammonium plus nitrate) content in the topsoil(0-15cm), soil water-field pore space, and soil temperature, determined in a study on clay loam and sandy loam at the pepper field in 2004. For comparisons between estimated and observed values of $N_2O$ emissions in the pepper field, it was investigated that $N_2O$ amount in the clay loam and sandy loam were overestimated as 12.2% and less estimated as 30%, respectively. However, $N_2O$ emissions were overestimated as 27.1% in the clay loam and 14.7% in the sandy loam from $N_2O$ gas samples collected once a week at the same time analyzing soil parameters. This modelling approach, based as it is well established and widely used soil measurements, has the potential to provide flux estimates from a much wider range of agricultural sites than would be possible by direct measurement of $N_2O$ emissions.

Effects of γ-Irradiation from Cobalt-60 on Immunogenicity of Eimeria tenella (Cobalt-60 γ-선 조사가 Eimeria tenella 의 면역원성에 미치는 영향)

  • Youn, Hee-jeong;Kang, Yung-bai;Jang, Du-hwan
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.657-664
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    • 1993
  • To reveal the immunogenicity of ${\gamma}-irradiated$ E tenella and its progeny, a series of experiments on the effects of Cobalt-to ${\gamma}-irradiation$ was performed. The SPF chickens inoculated with diffenrt doses of inoculum were challenged with $1{\times}10^5$ oocysts of virulent E tenella. The levels of 100 Gy ${\gamma}-irradiation$ from $^{60}Co$ and of inoculum with $1{\times}10^4$ oocysts were recognized as proper as immunogen by comparison of survival rates, body weight gains, blood in feces and lesion scores in the chickens. In these trials of challenge with virulent E tenella after inoculation with $1{\times}10^4$ oocysts of the ${\gamma}-irradiated$ E tenella and its progeny, the survival rates of the chickens challenged with the virulent E tenella after immunization with the 1st and the 3rd progeny groups of ${\gamma}-irradiated$ E tenella oocysts were higher(l00%) than that(87.0%) of the challenged control group. The signs of blood in feces and the lesion scores were seen markedly lower with the ourput of the smaller number of oocysts, i.e. OPG 103,900 and 25,800 in the groups of the 1st and the 3rd progeny, respectively, than those(OPG 1,658,900) of the challenged control group. The body weight gains of the 1st and the 3rd progeny groups, the 1st week and the 2nd week after challenge, were higher (2.6g and 155.4g, 11.6g and 168.9g respectively) than those(-85.8g and 63.6g, respectively) of the challenged control group, and the feed conversion ratios(FCR 3.28 and 2.96) of the 1st and the 3rd progeny groups were lower than that(FCR 5.60) of the groups challenged control group. The anticoccidial indices(70.5 and 93.9) of the groups challenged with the virulent oocysts of E tenella after immunization with the 1st and the 3rd progeny of the ${\gamma}-irradiated$ E tenella were significantly higher than that (ACI -81.9) of the challenged control group. It was thought that the immunogenicity of ${\gamma}-irradiated$ E tenella would be increase according to increase the number of generation passaged in chicken. That might be because of increasing the pathogenicity of ${\gamma}-irradiated$ E tenella according to increase the number of generation passaged in chicken.

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Effects of γ-Irradiation from Cobalt-60 on pathogenicity of Eimeria tenella (Cobalt-60 감마선 조사가 Eimeria tenella 의 병원성에 미치는 영향)

  • Youn, Hee-jeong;Kang, Young-bai;Jang, Du-hwan
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.649-655
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    • 1993
  • A series of experiments on the effects of ${\gamma}-irradiation$ was performed to reveal the pathogenicity of ${\gamma}-irradiated$ oocysts of E tenella from Cobalt-60 and its progeny. The SPF chickens were inoculated with differnt doses of radiation and inoculum. The level of 100 Gy ${\gamma}-irradiation$ from $^{60}Co$ and the level of inoculum with $1{\times}10^4$ oocysts were recognized more pathogenic than those of the other groups by comparison of body weight gains, blood in feces and lesion scores. The signs of blood in feces, lesion score and the number of excreted oocysts in the feces were revealed as the lowest in the group of the ${\gamma}-irradiated$ oocysts, the average in the group of the 1st and the 3rd progeny, and the highest in the group of non-irradiated oocysts of E $tenell\grave{a}$. The body weight gain of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was higher than those of the non-irradiated, the 1st and 3rd progeny groups. The body weight gain of the groups immunized with the 1st and the 3rd progeny of E tenella were higher than that of the non-irradiated group. The feed conversion ration of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was lower than those of the non-irradiated, the 1st and the 3rd progeny groups. The feed conversion ratios of the group immunized with the 1st and 3rd progeny of ${\gamma}-irradiated$ oocysts were lower than that of the group infected with non-irradiated E tenella. The anticoccidial index(ACI 190.6) in the chickens immunized with the ${\gamma}-irradiated$ oocysts of E tenella and those(ACI 142.8 and 107.4) of the 1st and the 3rd progeny groups were higher than that (ACT 87.4) of the group infected with non-irradiated E tenella. It was thought that the pathogenicity of ${\gamma}-irradiated$ E tenella would be recovered according to increase the number of generation passaged in chicken.

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Antitrust Regulation on the Restriction of Business Activities by Healthcare Providers' Organization (의료공급자 단체에 대한 공정거래법상 사업활동제한 적용 -달빛어린이 병원 사건을 중심으로-)

  • Jeong, Jae Hun
    • The Korean Society of Law and Medicine
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    • v.19 no.2
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    • pp.75-98
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    • 2018
  • Recently, the antitrust tribunal of Seoul High Court revoked the disposition of Korea Fair Trade Commission(hereafter 'KFTC'). While KFTC determined that the restriction of Korea Pediatrician Association violated article 26 of the Monopoly Regulation and Fair Trade Act(Korean antitrust law), Seoul High court viewed that KFTC failed to prove the compulsory measures and the restraint of competition required in article 26. The 'restriction' of article 26 should be interpreted as 'excessive restriction'. Since entrepeneurs' organization is allowed to limit its member's activities, KFTC could regulate entrepeneurs' organization on a very exceptional basis. In addition, though entrepeneurs' organization did not use compulsory measures to enforce its resolution, its 'excessive restriction' could fit into the notion of 'restriction' of article 26. Under the current medical care system, the price of medical care is decided by Korean government. Therefore the restriction of Korea Pediatrician Association is not likely to have effect on the price. However, the resolution of Korea Pediatrician Association was aimed to decrease the supply of medical care. Therefore the resolution is capable of having effect on the competition. In this sense, though KFTC failed to submit direct evidence to support the decrease of quantity, there could be possibility of restraint of competition. The Seoul High Court's decision has important implications. The leading case on restraint of competition(Supreme Court 2002Du8628, Posco case) was delivered in 2007. However the remaining issue such as the standard and scope of restraint of competition is not clear. Through reappeal case of this decision, Supreme Court has to decide the line between competition and its restraint.

Prevalence Rate of Lead Related Subjective Symptoms in Lead Workers (연취급 근로자의 연폭로 수준에 따른 주관적 자각증상 호소율)

  • Jeong, Du-Shin;Kim, Hwa-Sung;Ahn, Kyu-Dong;Lee, Byung-Kook
    • Journal of Preventive Medicine and Public Health
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    • v.26 no.2 s.42
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    • pp.251-267
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    • 1993
  • The relationship between lead related subject symptoms and lead exposure indices was studied in 435 male lead workers in thirteen lead using industries. 212 male office workers who were not exposed to lead occupationally were also studied as a control group. Fourteen lead related symptoms were selected. They were further subdivied into 4 sub-symptom groups such as 1) gastrointestinal, 2) neuromuscular and joint 3) constitutional, and 4) psychological symptoms. Symptom questionnaires were provided to the workers and filled up by themselves and reconfirmed by interviewer(doctor). The test used fer the evaluation of lead exposure were blood lead(PbB), zinc protoporphyrin in whole blood(ZPP), hemoglobin(Hb), hematocrit (Hct), delta-aminolevulinic acid in urine(DALA). The results obtained were as follows; 1. The higher prevalence rate in the sub-group of neuromuscular and joint symptoms was observed in occupationally lead exposed subjects than non-exposed subjects. Among the sub-groups, the most frequent symptom was 'numbness of finger, hands or feet', and the prevalence of the symptom of 'arthralgia', 'weakness of fingers, hands or feet' and 'myalgia' were higher in order. 2. While the symptom which showed the biggest difference of prevalence rate among the 14 symptoms between exposed and non-exposed subjects was 'numbness of fingers, hands or feet', the symptom which showed the highest prevalence rate was 'feeling tired generally' in exposed and non-exposed subjects, but no statistical difference of symptom prevalence were observed. 3. In total study population, PbB and ZPP had dose-response relationship with 4 symtoms of neuromuscular and joint symptoms ('numbness of finger, hands or feet', 'arthralgia', 'weakness of fingers, hands or feet' and 'myalgia') and one symptom of gastrointestinal group('intermittent pains in lower abdomen'). 4. In lead exposed workers, only neuromuscular and joint symptoms group showed dose-response relationship with PbB and ZPP, 5. In lead exposed workers, the prevalance rate of overall symptoms of lead workers with age below 39 years was higher than that of lead workers with age above 40. While neuromuscular and joint symptoms group had a dose-response relationship with PbB in former group, it had a dose-response relationship with ZPP in latter group. 6. Age adjusted odds ratios of symptoms of non-exposed with exposed and odds ratios of low exposed with high exposed workers showed the dose-response relationship of lead exposure with neuromuscular and joint symptoms group('numbness of fingers, hands or feet', 'arthralgia', 'weakness of fingers, hands or feet' and 'myalgia') and gastrointestinal symptoms group('intermittent pains in lower abdoman').

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Effect of gomchwi (Ligularia fischeri) extract against high glucose- and H2O2-induced oxidative stress in PC12 cells (PC12 신경세포에서 고당 및 과산화수소로 유도된 산화적 스트레스에 대한 곰취 추출물의 효과)

  • Park, Sang Hyun;Park, Seon Kyeong;Ha, Jeong Su;Lee, Du Sang;Kang, Jin Yong;Kim, Jong Min;Lee, Uk;Heo, Ho Jin
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.508-514
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    • 2016
  • Effects of the ethyl acetate fraction from gomchwi (Ligularia fischeri) extract against high $glucose/H_2O_2-induced$ oxidative stress and in vitro neurodegeneration were investigated to confirm the physiological property of the extract. The ethyl acetate fraction of gomchwi extract showed the highest total phenolic contents than the other solvent fractions. An anti-hyperglycemic activity of the ethyl acetate fraction was evaluated using the ${\alpha}-glucosidase$ inhibitory assay, and the half maximal inhibitory concentration ($IC_{50}$) value for ${\alpha}-glucosidase$ was found to be $727.64{\mu}g/mL$. In addition, the ethyl acetate fraction showed excellent 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt radical scavenging activity, and inhibition of malondialdehyde production. The ethyl acetate fraction also decreased intracellular reactive oxygen species, whereas neuronal cell viability against high glucose/$H_2O_2$-induced cytotoxicity was found to be increased. Finally, 3,5-dicaffeoylquinic acid as a main phenolic compound in the ethyl acetate fraction was analyzed by high-performance liquid chromatography. These results suggest that gomchwi might be a good natural source of functional materials to prevent diabetic neurodegeneration.

Breed Effects of Terminal Sires on Carcass Traits and Meat Quality in Commercial Pig Industry (돼지의 웅돈계열에 따른 도체형질 및 육질 변화에 관한 연구)

  • Sun, Du-Won;Seo, Hyun-Woo;Kim, Byeong-Woo;Yang, Han-Sul;Joo, Seon-Tea;Lee, Jung-Gyu
    • Food Science of Animal Resources
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    • v.33 no.1
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    • pp.125-132
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    • 2013
  • In the present study, to determine the effects of terminal sire strains, carcass characteristics, yields of retail cut, and meat quality of 425 pigs were examined. Terminal sires were divided into five strains (D1, D2, B1, B2, and BD) based on the countries of origin and breeds. The strains D1 and D2 showed high grade 1+ appearance ratios and strains B1, B2, and D1 showed high appearance ratios of carcass yield grade A. As for the real retail cut yields of meat, strain D1 showed large real retail cut yields of meat in shoulder, rib, fore leg, loin, belly, tenderloin and hind leg. Moisture contents were significantly higher in strain B1, and protein and fat contents were significantly higher for strain D1 than in the other strains (p<0.05). Shear force values were significantly higher for strain B1 than in the other strains (p<0.05). For meat color properties, the lightness were significantly higher in strains D1, D2, and BD, and the yellowness were significantly higher in strains D1, D2, and BD relative to the other strains (p<0.05). In sensory evaluation, drip of strains D1 and D2 were significantly lower while marbling and overall acceptability were significantly higher in the other four strains (p<0.05). Therefore, when considering the results of the present study comprehensively, using strains D1 and D2 that showed high grade 1+ appearance ratios, large real retail cut yields of belly, significantly higher lightness and sensory evaluation will be helpful to the incomes of producers and farm households.

In vitro Anti-hypertensive, Antioxidant and Anticoagulant Activities of Extracts from Haliotis discus hannai (전복(Haliotis discus hannai) 추출물의 혈압강하, 항산화능 및 항혈전능에 대한 in vitro 효과)

  • Kim, Hag-Lyeol;Kang, Seong-Gook;Kim, In-Chul;Kim, Seon-Jae;Kim, Du-Woon;Ma, Seung-Jin;Gao, Tiancheng;Li, Hua;Kim, Min-Jung;Lee, Tae-Hoon;Ham, Kyung-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.835-840
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    • 2006
  • The purpose of this study was to examine the in vitro effects of body and visceral portion of Haliotis discus hannai on angiotensin converting enzyme (ACE) activity and antioxidant and anticoagulant capacity. Extracts from both abalone body and visceral portion using 80% ethanol showed a high ACE-inhibitory effect. While ACE-inhibitory effect of extracts of the body part was dose-dependent, visceral extracts did not show any difference by the level of concentration. ACE-inhibitory effect of the visceral portion was much higher than that of the body. Antioxidant capacity was increased with increasing concentration of 80% ethanol body extracts although the capacity was low. The 80% ethanol visceral extracts showed a similar level of antioxidant capacity to the body extract in low concentration. Water extracts showed a dose-dependent increase in the activity. There was no significant difference in the antioxidant activity between the body and the visceral part. Anticoagulant capacity of 80% ethanol extracts, which was measured using prothrombin time(PT), was higher in the body part than the visceral part. Water extracts of Haliotis discus showed no any significant effect on anticoagulant capacity. The in vitro effects were also examined after Haliotis discus was refrigerated for 48 hours. Higher ACE-inhibitory effect was observed for the visceral portion than the body, in particular, before the sample was refrigerated. Antioxidant effect of Haliotis discus increased with increasing level of the sample before it was refrigerated. However, there was a significant difference between the body and the visceral part, which showed significantly higher capacity. There was no significant difference between the body and visceral part in PT regardless of refrigeration. While activated partial thromboplastin time (APTT) showed no significant difference between body and visceral part, there was a significant difference in the capacity between before and after the refrigeration, which showed much lower coagulant capacity.

Studies on the Energy and Protein Requirement Determination for Broiler Production -4. Effect of Iso-calorie and Different Protein Level on Broiler Production- (Broiler 생산(生産)에 있어서 Energy와 Protein 요구량(要求量) 결정(決定)에 관한 연구(硏究) (제4보(第4報)) -동일(同一)Energy수준(水準)에서의 Protein 수준이 Broiler 생산(生産)에 미치는 영향(影響)-)

  • Kim, Dae Jin;Kim, Young Kil;Ko, Yong Du
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.395-400
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    • 1983
  • The experiment was conducted to study the effect of protein level on weight gain, feed consumption and the efficiencies of feed, energy and protein when the broiler chick was fed the diet of different protein level with the iso-calorie content. The energy and protein level of the basal ration was 3200 kcal and 22.85% for starter period and 3265 Kcal and 18.58% for finisher period, respectively. In order to increase the protein level of the experimental diet, the basal diet was substituted by fish meal by the ratio of 3, 6, 9, and 12%. The male Abor Acre broiler chicks were employed for 8 weeks and the result obtained were as follows. 1. The weekly body weight gain was significantly greater for Diet C than for Diet A and E in the starter period and greater for Diet A than for Diet E in the finisher period (P<0.05). 2. In stater period the feed intake was not affected by the protein level but in finisher period the feed intake for Diet C, D, and E was significantly reduced compared with Diet A. 3. As for feed conversion ratio, Diet C of 1.43 was improved significantly compared with Diet A of 1.58(P<0.05). 4. As the protein level of diet increased, the energy efficiency ratio increased and however, the protein efficiency ratio decreased in starter and finisher period. In conclusion the best performance of broiler is thought to be realized when the protein and energy level was 23% and 3200 Kcal for starter period and 20% and 3250 Kcal for finisher period, respectively.

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