• 한국발생생물학회지:발생과생식
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• 제20권2호
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• pp.141-147
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• 2016

Rad51 is a key component of homologous recombination (HR) to repair DNA double-strand breaks and it forms Rad51 recombinase filaments of broken single-stranded DNA to promote HR. In addition to its role in DNA repair and cell cycle progression, Rad51 contributes to the reprogramming process during the generation of induced pluripotent stem cells. In light of this, we performed reprogramming experiments to examine the effect of co-expression of Rad51 and four reprogramming factors, Oct4, Sox2, Klf4, and c-Myc, on the reprogramming efficiency. Co-expression of Rad51 significantly increased the numbers of alkaline phosphatase-positive colonies and embryonic stem cell-like colonies during the process of reprogramming. Co-expression ofRad51 significantly increased the expression of epithelial markers at an early stage of reprogramming compared with control cells. Phosphorylated histone H2AX (${\gamma}H2AX$), which initiates the DNA double-strand break repair system, was highly accumulated in reprogramming intermediates upon co-expression of Rad51. This study identified a novel role of Rad51 in enhancing the reprogramming efficiency, possibly by facilitating mesenchymal-to-epithelial transition and by regulating a DNA damage repair pathway during the early phase of the reprogramming process.

• 한국지능시스템학회논문지
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• 제23권5호
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• pp.460-465
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• 2013

본 논문은 실내외 공간에서 인간을 포한함 이동물체의 위치를 인식하고 출입금지 구역으로의 이동에 대해서 보안목적의 이동로봇이 센서를 이용하여 이동물체 및 인간의 행동 움직임을 인식하고 진입을 제한하는 주행기법을 제시하고 있다. 제시한 방법은 로봇자체의 DR센서 정보와 레이져스케너에서 얻은 환경정보로부터 로봇의 위치추정방법을 결합 한 것이다. 이동로봇은 인간의 속도벡터를 계산 및 주행할 경로를 계획하고 인간의 진행방향을 차단할 수 있도록 예측된 경로를 따라 주행을 하게 된다. 이때, 인간의 움직임은 포인터 물체로 간주하였으며 로봇의 기구학에 기반하여 인간의 위치를 추정하는 기본 방법을 제시하고 그 타당성을 검정하기위해 로봇을 이용한 위치추정 및 추적 실험결과를 제시하였다.

• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.9-16
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• 2013

Coactivator-associated arginine methyltransferase 1 (CARM1) is included in the protein arginine methyltransferase (PRMT) family, which methylates histone arginine residues through posttranslational modification. It has been proposed that CARM1 may up-regulate the expression of pluripotency-related genes through the alteration of the chromatin structure. Mouse embryonic stem cells (mESCs) are pluripotent and have the ability to self-renew. The cells are mainly used to study the genetic function of novel genes, because the cells facilitate the transmission of the manipulated genes into target mice. Since the up-regulated methylation levels of histone arginine residue lead to the maintenance of pluripotency in embryos and stem cells, it may be suggested that CARM1 overexpressing mESCs elevate the expression of pluripotency-related genes in reconstituted embryos for transgenic mice and may resist the differentiation into trophectoderm (TE). We constructed a fusion protein by connecting CARM1 and 7X-arginine (R7). As a cell-penetrating peptide (CPP), can translocate CARM1 protein into mESCs. CPP-CARM1 protein was detected in the nuclei of the mESCs after a treatment of 24 hours. Accordingly, the expression of pluripotency-related genes was up-regulated in CPP-CARM1-treated mESCs. In addition, CPP-CARM1-treated mESC-derived embryoid bodies (EBs) showed an elevated expression of pluripotency-related genes and delayed spontaneous differentiation. This result suggests that the treatment of recombinant CPP-CARM1 protein elevates the expression of pluripotency-related genes of mESCs by epigenetic modification, and this protein-delivery system could be used to modify embryonic fate in reconstituted embryos with mESCs.

• 한국발생생물학회지:발생과생식
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• 제23권4호
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• pp.333-344
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• 2019

In vertebrate reproductive system, estrogen receptor (ER) plays a pivotal role in mediation of estrogenic signaling pathways. In the present study, we report the cDNA cloning, expression analysis, and transcriptional activity of ERβ1 subtype from medaka Oryzias dancena. The deduced O. dancena ERβ1 (odERβ1; 519 amino acids) contained six characteristic A/B to E/F domains with very short activation function 2 region (called AF2). A phylogenetic analysis indicated that odERβ1 was highly conserved among teleost ERβ1 subgroup. A conventional RT-PCR revealed that the odERβ1 transcripts were widely distributed in the multiple tissues, the ovary, brain, gill, intestine, kidney, and muscle. Further, the relatively higher odERβ1 expressions in the ovary and brain were clearly reproduced in RT-qPCR assay. When HA-fused odERβ1 expression vector was transfected into HEK293 cells, an immunoreactivity for odERβ1 was mainly detected in the nucleus part. Finally, an estrogen responsive element driven luciferase reporter assays demonstrated that the transcriptional activity of odERβ1 significantly increased by estradiol-17β (E2) in a dose dependent manner (p<0.05). However, fold-activation of odERβ1 in the presence of E2 was markedly weak, when it compared with those of O. latipes ERβ1. Taken together, these data suggest that odERβ1 represents a functional variant of teleost ERβ subtype and provides a basic tool allowing future studies examining the function of F domain of ERβ1 subtype and expanding our knowledge of ERβ evolution.

• 한국발생생물학회지:발생과생식
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• 제23권4호
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• pp.367-375
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• 2019

Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.181-187
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• 2015

Early life stage mortality in fish is one of the problems faced by loach aquaculture. However, our understanding of immune system in early life stage fish is still incomplete, and the information available is restricted to a few fish species. In the present work, we investigated the expression of immune-related transcripts in loach during early development. In fishes, recombination-activating gene 1 (RAG-1) and sacsin (SACS) have been considered as immunological function. In this study, the expression of the both genes was assessed throughout the early developmental stages of loach using real-time PCR method. maRAG-1 mRNA was first detected in 0 dph, observed the increased mostly until 40 dph. Significant expression of maRAG-1 was detected in 0 to 40 dph. These patterns of expression may suggest that the loach start to develop its function after hatching. On the other hand, maSACS was detected in unfertilized oocyte to molura stages and 0 to 40 dph. maSACS mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred.

• 한국측량학회지
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• 제19권1호
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• pp.95-106
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• 2001

본 연구에서는 북한 서해안 지역의 농지기반조성현황을 조사하기 위하여 Landsat TM. JERS OPS, SPOT PAN데이터를 주로 사용하는 원격탐사기법과 시대별 진척상황을 추적하기 위하여 발행년도가 다른 지도 등을 이용하였다. 농업시설물을 판독하기 위하여 기하보정, 디지털 모자이킹 , 영상중합, 선형추출, 토지이용분류 등의 기법을 연구하였으며, 이를 응용하여 북한 서해안지역에 계획되어 있는 간척가능지구, 미완공간척지, 수리계획 등을 조사하였다. 확인된 간척가능 면적은 완공 또는 부분 완공되었거나 방조제노선이 계획된 지구를 합한 총면적이 177,766ha로서 북한이 발표한 30만 ha의 59%에 해당된다. 또한, 1994년도 $\boxDr$북한의 동향$\boxUl$에서 발표한 제3차 7개년 계획기간(1987-1993)에 북한정부가 추진한 총 면적 27,100ha의 간척지 개발면적을 조사한 결과, 기 개발된 면적 16,555 ha와 미완공 간척지면적 16,826 ha로 나타났다. 이들 간척지구는 해안지역에 위치하고 자체유역이 작아 용수가 부족한 실정으로 서해안으로 흐르는 압록강, 청천강, 대동강, 예성강 등으로부터 용수공급을 받는 2000리 물길사업과 연계되어 있으나 용수의 과부족은 검토를 요한다.

• Nutrition Research and Practice
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• 제7권6호
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• pp.510-518
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• 2013

This study aims to demonstrate the effect of farming technology on introducing medicinal plants (MP) and wild food plants (WFP) into a traditional agricultural system within peri-urban zones. Field investigations and semi-structured focus group interviews conducted in the Buhozi community showed that 27 health and nutrition problems dominated in the community, and could be treated with 86 domestic plant species. The selected domestic MP and WFP species were collected in the broad neighboring areas of the Buhozi site, and introduced to the experimental field of beans and maize crops in Buhozi. Among the 86 plants introduced, 37 species are confirmed as having both medicinal and nutritional properties, 47 species with medicinal, and 2 species with nutritional properties. The field is arranged in a way that living hedges made from Tithonia diversifolia provide bio-fertilizers to the plants growing along the hedges. The harvest of farming crops does not disturb the MP or WFP, and vice-versa. After harvesting the integrated plants, the community could gain about 40 times higher income, than from harvesting farming crops only. This kind of field may be used throughout the year, to provide both natural medicines and foods. It may therefore contribute to increasing small-scale crop producers' livelihood, while promoting biodiversity conservation. This model needs to be deeply documented, for further pharmaceutical and nutritional use.

• 한국축산식품학회지
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• 제23권2호
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• pp.186-192
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• 2003

최근 항산화제 연구는 식품, 의약품, 농업분야 등 다방면에서 이용될 수 있기 때문에 많은 산업적 효과를 기대할 수 있다. 특히 지금까지 알려진 항산화제가 약한 활성, 독성 및 사용상의 한계로 인하여 사용하는데 있어서 많은 문제점을 내포하고 있다. 따라서 천연으로부터 보다 안전하고 강한 활성을 지닌 천연항산화제의 개발이 요구된다. 활성산소 제거능력이 향상된 유산균은 식품산업에 중요하며 인간 장내 외인성, 내인성 산화적 스트레스 제거에 중요하다고 생각된다. 따라서 유산균을 이용한 항산화제의 고부가가치 창출을 위해서는 생물학적 기능연구 및 질병모델계에서의 효능평가가 이루어져야 하며 항산화제의 효능검정 및 항산화제 작용 메커니즘 등 다양한 방면의 연구가 병행되어야 할 것으로 생각된다

• 한국콘텐츠학회논문지
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• 제10권12호
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• pp.328-341
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• 2010

국내 화물자동차운송업은 국가의 물류 기본산업임에도 불구하고 많은 문제점을 안고 있으며, 물류대란과 같은 국가적인 화물운송체계의 위기를 만들기도 하였다. 본 연구의 목적은 국내 화물자동차운송업의 효율성을 DEA기법으로 분석함으로써 벤치마킹의 대상이 될 수 있는 국내 화물자동차운송업을 분석하였으며, 비효율적인 기업이 개선시켜야하는 투사값을 제시함으로써 기업의 효율성을 위한 수치를 제공하고자 한다. 분석결과 총 30개 화물자동차운송업들의 CCR-I, BCC-I 효율성과 규모수익성(RTS)을 평가하였다. 또한 분석된 자료를 바탕으로 벤치마킹의 대상이 될 수 있는 화물자동차운송기업을 제시하였다. 분석결과 CCR-I효율성이 1인 업체는 7개 기업, BCC-I효율성이 1인 업체는 15개 기업으로 분석되었다. 규모수익성은 IRS가 16개의 기업, DRS가 5개 기업, CRS가 9개 기업으로 분석되었다.