• Title/Summary/Keyword: DPR

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Fabrication of Nano Dot and Line Arrays Using NSOM Lithography

  • Kwon Sangjin;Kim Pilgyu;Jeong Sungho;Chang Wonseok;Chun Chaemin;Kim Dong-Yu
    • Journal of the Optical Society of Korea
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    • v.9 no.1
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    • pp.16-21
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    • 2005
  • Using a cantilever type nanoprobe having a 100㎚m aperture at the apex of the pyramidal tip of a near-field scanning optical microscope (NSOM), nanopatterning of polymer films are conducted. Two different types of polymer, namely a positive photoresist (DPR-i5500) and an azopolymer (Poly disperse orange-3), spincoated on a silicon wafer are used as the substrate. A He-Cd laser with a wavelength of 442㎚ is employed as the illumination source. The optical near-field produced at the tip of the nanoprobe induces a photochemical reaction on the irradiated region, leading to the fabrication of nanostructures below the diffraction limit of the laser light. By controlling the process parameters properly, nanopatterns as small as 100㎚ are produced on both the photoresist and azopolymer samples. The shape and size variations of the nanopatterns are examined with respect to the key process parameters such as laser beam power, irradiation time or scanning speed of the probe, operation modes of the NSOM (DC and AC modes), etc. The characteristic features during the fabrication of ordered structures such as dot or line arrays using NSOM lithography are investigated. Not only the direct writing of nano array structures on the polymer films but also the fabrication of NSOM-written patterns on the silicon substrate were investigated by introducing a passivation layer over the silicon surface. Possible application of thereby developed NSOM lithography technology to the fabrication of data storage is discussed.

C9orf72-Associated Arginine-Rich Dipeptide Repeat Proteins Reduce the Number of Golgi Outposts and Dendritic Branches in Drosophila Neurons

  • Park, Jeong Hyang;Chung, Chang Geon;Seo, Jinsoo;Lee, Byung-Hoon;Lee, Young-Sam;Kweon, Jung Hyun;Lee, Sung Bae
    • Molecules and Cells
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    • v.43 no.9
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    • pp.821-830
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    • 2020
  • Altered dendritic morphology is frequently observed in various neurological disorders including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), but the cellular and molecular basis underlying these pathogenic dendritic abnormalities remains largely unclear. In this study, we investigated dendritic morphological defects caused by dipeptide repeat protein (DPR) toxicity associated with G4C2 expansion mutation of C9orf72 (the leading genetic cause of ALS and FTD) in Drosophila neurons and characterized the underlying pathogenic mechanisms. Among the five DPRs produced by repeat-associated non-ATG translation of G4C2 repeats, we found that arginine-rich DPRs (PR and GR) led to the most significant reduction in dendritic branches and plasma membrane (PM) supply in Class IV dendritic arborization (C4 da) neurons. Furthermore, expression of PR and GR reduced the number of Golgi outposts (GOPs) in dendrites. In Drosophila brains, expression of PR, but not GR, led to a significant reduction in the mRNA level of CrebA, a transcription factor regulating the formation of GOPs. Overexpressing CrebA in PR-expressing C4 da neurons mitigated PM supply defects and restored the number of GOPs, but the number of dendritic branches remained unchanged, suggesting that other molecules besides CrebA may be involved in dendritic branching. Taken together, our results provide valuable insight into the understanding of dendritic pathology associated with C9-ALS/FTD.

Assessment of population structure and genetic diversity of German Angora rabbit through pedigree analysis

  • Abdul Rahim;K. S. Rajaravindra;Om Hari Chaturvedi;S. R. Sharma
    • Animal Bioscience
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    • v.36 no.5
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    • pp.692-703
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    • 2023
  • Objective: The main goals of this investigation were to i) assess the population structure and genetic diversity and ii) determine the efficiency of the ongoing breeding program in a closed flock of Angora rabbits through pedigree analysis. Methods: The pedigree records of 6,145 animals, born between 1996 to 2020 at NTRS, ICAR-CSWRI, Garsa were analyzed using ENDOG version 4.8 software package. The genealogical information, genetic conservation index and parameters based on gene origin probabilities were estimated. Results: Analysis revealed that, 99.09% of the kits had both parents recorded in the whole dataset. The completeness levels for the whole pedigree were 99.12%, 97.12%, 90.66%, 82.49%, and 74.11% for the 1st, 2nd, 3rd, 4th, and 5th generations, respectively, reflecting well-maintained pedigree records. The maximum inbreeding, average inbreeding and relatedness were 36.96%, 8.07%, and 15.82%, respectively. The mean maximum, mean equivalent and mean completed generations were 10.28, 7.91, and 5.51 with 0.85%, 1.19%, and 1.85% increase in inbreeding, respectively. The effective population size estimated from maximum, equivalent and complete generations were 58.50, 27.05, and 42.08, respectively. Only 1.51% of total mating was highly inbred. The effective population size computed via the individual increase in inbreeding was 42.83. The effective numbers of founders (fe), ancestors (fa), founder genomes (fg) and non-founder genomes (fng) were 18, 16, 6.22, and 9.50, respectively. The fe/fa ratio was 1.12, indicating occasional bottlenecks had occurred in the population. The six most influential ancestors explained 50% of genes contributed to the gene pool. The average generation interval was 1.51 years and was longer for the sire-offspring pathway. The population lost 8% genetic diversity over time, however, considerable genetic variability still existed in the closed Angora population. Conclusion: This study provides important and practical insights to manage and maintain the genetic variability within the individual flock and the entire population.

Proposing Research and Development Activities for Utilizing the Global Precipitation Measurement (GPM) (전구강수관측(GPM) 활용을 위한 제언)

  • Sohn, Byung-Ju;Nam, Jae-Cheol;Park, Seon-Ki;Ahn, Myung-Hwan;Yoo, Jung-Moon;Lee, Hee-Sang;Chang, Dong-Eon;Ho, Chang-Hoi;Bae, Deg-Hyo;Kim, Seong-Jun;Oh, Hyun-Jong;Park, Seong-Chan;Kim, Ju-Hong
    • Atmosphere
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    • v.15 no.1
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    • pp.47-57
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    • 2005
  • Extending the success of the Tropical Rainfall Measuring Mission (TRMM), the spaceborne measurement of precipitation by Global Precipitation Measurement (GPM) is initiated. The GPM consists of a core satellite which will have a dual-frequency precipitation radar (DPR) and a constellation of small satellites equipped with microwave radiometers. The GPM is inherently a global program. Responding to the GPM plan, many other nations are much interested in participating in the GPM team or simply utilizing GPM products aiming at the development of meteorological technology. Korea can fully function its role if Korea is selected as a CAL/VAL site for the GPM because Korea maintains a well-established dense rain gauge network (AWS), precipitation radars, and the Haenam super site for surface observation. In this feasibility study, the necessities of the GPM project in the context of academical and social backgrounds and associated international and domestic activities are investigated. And GPM-related core technologies and application areas are defined. As a result, it is found that GPM will represent a great opportunity for us because of its ability to provide not only much enhanced three-hourly global rain products but also very useful tools for the enhancement of weather forecasting capabilities, management of water resources, development and implementation of monitoring techniques for severe weather phenomena, agricultural managements and climate application. Furthermore, rain retrieval and CAL/VAL technologies obtained during the involvement in the international GPM project will serve as basic knowledges to run our own geostationary satellite program.

Characteristics of Growth and Leaves Component Contents of in One Year Old Tetraploid Robinia pesudoacacia at Sangwon Area in Pyongyang, DPR Korea (북한 평양 상원지역에서 1년생 4배체 아까시나무의 생장특성과 엽성분 함량)

  • Jin, Yonghuan;Kang, Ho-Sang;Park, Go-Eun;Lee, Don-Koo
    • Korean Journal of Environment and Ecology
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    • v.25 no.5
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    • pp.710-716
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    • 2011
  • The growth characteristics and the several component content of leaves in one year old tetraploid of Robinia pseudoacacia introduced at five site planted respectively were surveyed in Sangwon area Pyongyang, and the expand plant possibility and the leaves harvested as feed additive use of possibility in Democratic People's Republic of Korea was discussed in comparison with tetraploid of R. pseudoacacia of Yanqing area. The results were as follows (1) the length and stump diameter of annual sprouting stems at Sangwon area were smaller 50% than the tetraploiod of R. pseudoacacia stems that Yanqing area; (2) the length of sprouting stems in slope sites of Eastern (E), Western(W) and Southern(S) at Sangwon were higher 23~30% than the length that Northern slope site(N), and the diameters of sprouting stem in the slope of Eastern(E) and Southern(S) were higher 14~23% than the slope sites of Northern(N) and Western(W); (3) crude protein contents and ash contents of leaves at Sangwon were higher 40% general R. pseudoacacia and similar to the leaves that Yanqing area.