• 생명과학회지
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• 제24권11호
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• pp.1217-1223
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• 2014

본 연구는 찔레의 열수추출물과 에탄올추출물간의 항산화 활성 비교를 위해 in vitro에서 총 페놀 및 플라보노이드 함량을 측정하였으며, DPPH, ABTS, NO radical 소거능 및 환원력을 $10{\sim}500{\mu}g/ml$의 농도에서 실험하였다. DPPH radical 소거능은 열수추출물(4.95~92.15%)과 에탄올추출물(2.76~90.55%)이 모두 농도 의존적으로 유의성 있게 높아졌으며 열수추출물이 더 높은 소거능을 보였다. ABTS radical 소거능을 비교한 결과 열수추출물(4.44~92.99%)이 에탄올추출물(2.63~87.12%)에 비해 유의적으로 높은 소거활성을 나타내었다. 환원력은 열수추출물(0.256~0.775)이 에탄올추출물(0.234~0.568)에 비해 유의적으로 높은 활성을, 그리고 농도 의존적으로 높아지는 것을 보였으며, NO radical 소거능을 비교한 결과 열수추출물이 에탄올추출물보다 조금 높은 활성을 보였고 농도 의존적으로 높아지는 것을 확인하였다. 찔레추출물의 항산화력의 $IC_{50}$값을 비교한 결과 DPPH radical 소거능, ABTS radical 소거능에서 각각 열수추출물에서 79.73, $79.82{\mu}g/ml$으로 나타났고, 에탄올추출물의 경우 145.85, $159.03{\mu}g/ml$로 나타났다. 총 페놀함량은 열수추출물에서 140.74 mg/g, 에탄올추출물에서 37.83 mg/g 플라보노이드 함량은 열수추출물에서 45.31 mg/g, 에탄올추출물에서 42.68 mg/g로 에탄올 추출물에 비해 열수추출물이 높게 나타났다. 결론적으로 찔레꽃 추출물은 항산화 효능을 나타내는 실험들에서 모두 높은 수치를 나타내어 천연 항산화제로 이용가치가 높다고 사료된다.

• 한방비만학회지
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• 제9권2호
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• pp.57-63
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• 2009

In recent year, We are concerned about anti-aging, disease-protection, long-life, many methods are used in solving this problem. And Those are related with antioxidative ability. Recently, We heard that Cucurbita moschata Duch. has anti-hypertensive effect and good for health. So I let made a experiment for this result of the anti-oxidant effect of Cucurbita moschata Duch. used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity and Bovine serum albumin(BSA). The results of this study were as follows: We measured level of DPPH radical scavenging activity. And we obtained results that the ability of DPPH radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $20\;mg/m{\ell}$. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $10\;mg/m{\ell}$. When we inspected antioxidation with Bovine serum albumin(BSA). And we obtained that antioxidative ability was increased after$2.5\;mg/m{\ell}$ of Cucurbita moschata Duch.. So I guess that Cucurbita moschata Duch. has effect of antioxidative ability. Hereafter we need differential experimental methods for evidence of antioxidative effect on Cucurbita moschata Duch.

• 화훼연구
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• 제26권4호
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• pp.202-208
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• 2018

본 연구는 금잔화를 꽃차로 이용하기 위해 열풍 건조 처리 시간에 따른 항산화 물질함량 및 항산화 활성을 분석하고, 최적 처리 시간을 구명하기 위해 수행하였다. 식용꽃 금잔화를 $60^{\circ}C$에서 5시간, 10시간, 15시간 열풍건조 한 뒤 Hunter value를 측정하고 95% 에탄올을 용매로 하여 추출하였다. 항산화 물질인 총 폴리페놀, 총 플라보노이드, 총 카로티노이드 함량과 DPPH radical 소거 활성을 통한 항산화 활성, 이를 통한 항산화 물질 함량과 항산화 활성 간의 상관관계를 분석하였다. 처리시간이 증가함에 따라 Hunter value L 값과 +b값이 모두 감소하는 경향을 보였으며, 15시간 처리구에서 다소 많이 감소하였다. 또한, 항산화 물질 함량을 조사한 결과, 총 폴리페놀 함량은 처리 시간에 따른 차이는 없었으나 총 플라보노이드 함량과 총 카로티노이드 함량은 5시간과 10시간 처리 시 함량이 다소 높았으며, 처리 시간이 증가함에 따라 감소하여 15시간 처리시 대조구와 차이가 없는 것으로 조사되었다. DPPH radical 소거 활성 분석 결과, 5시간과 10시간 처리시 대조구보다 활성이 높았으며, 총 카로티노이드가 DPPH radical 소거 활성 간의 상관관계가 높은 것으로 나타났다($p{\leq}0.01$, r=0.610). 따라서, 열풍건조한 금잔화는 총 카로티노이드가 항산화 활성에 기여하며, 5시간 및 10시간 열풍건조 처리가 금잔화의 항산화 물질 함량 및 활성 증가에 효과적인 것으로 판단되나 식물의 열처리 가공에 있어 오랜 시간은 내부 조직 및 항산화 물질 파괴에 영향을 미칠 수 있기 때문에 금잔화 열풍건조 처리 시 5시간이 효과적일 것으로 판단된다.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.673-682
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• 2017

식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수 분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 연산오계란 단백질 가수 분해물을 Ultrafiltration를 이용하여 HDS(분획되지 않은 가수 분해물), 1 kDa, 5 kDa, 10 kDa, 50 kDa로 분획된 기능성 펩타이드의 DPPH radical scavenging activity, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity 및 $Fe^{2+}$ chelation ability을 평가하였다. 그 결과 DPPH radical scavenging activity 최대값은 1 kDa(70.83 %), hydroxyl radical scavenging activity 최대값은 5 kDa (47.01 %), superoxide anion radical scavenging activity 최대값은 5 kDa(40.57 %), $Fe^{2+}$ chelation ability 최대값은 5 kDa(29.87 %)로 나타났다. Ultrafiltration를 이용하여 fractionation된 단백질 가수 분해물의 항산화 저해 능력 $IC_{50}$ 평가하였다. 그 결과 HDS의 최대값은 superoxide anion radical scavenging activity($IC_{50}$, 5.42 mg/ml)이고, 1 kDa의 최대값은 $Fe^{2+}$ chelation ability($IC_{50}$, 1.67 mg/ml)이고, 5 kDa의 최대값은 $Fe^{2+}$ chelation ability($IC_{50}$, 2.09 mg/ml)이고, 10 kDa의 최대값은 $Fe^{2+}$ chelation ability($IC_{50}$, 2.61 mg/ml)이고, 50 kDa의 최대값은 $Fe^{2+}$ chelation ability($IC_{50}$, 4.53 mg/ml)이다. 그러므로 본 연구 결과를 바탕으로 5 kDa를 이용하여 오계란 단백질에서 분획한 펩타이드는 항산화 기능성 식품소재로서 활용할 가치가 높을 것으로 기대한다.

• Journal of Ginseng Research
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• 제35권1호
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• pp.60-68
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• 2011

The chemical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity changes of ginsenoside $Rb_1$-glycine and ginsenoside $Rg_1$-glycine mixtures by Maillard reaction were investigated to identify the role of Maillard reaction in the increased antioxidant activity of ginseng by heat-processing. The DPPH radical scavenging activity of $Rg_1$-glycine mixture was more strongly increased by heat-processing than that of $Rb_1$-glycine mixture. From the analyses of ginsenosides, $Rb_1$ was gradually changed into 20(S)-$Rg_3$, 20(R)-$Rg_3$, $Rk_1$ and $Rg_5$ by heat-processing. $Rg_1$ was gradually changed into 20(S)-$Rh_1$, 20(R)-$Rh_1$, $Rk_3$ and $Rh_4$ by heat-processing. However, the generation of these less-polar ginsenosides was not related to the increased DPPH radical scavenging activity of $Rb_1$-glycine and $Rg_1$-glycine mixtures because their DPPH radical scavenging activities were already significantly increased when dried at $50^{\circ}C$, which temperature induce no structural changes of ginsenosides. In the comparison of browning compound levels of $Rg_1$-glycine and $Rb_1$-glycine mixtures, the extents of Maillard reaction were positively correlated with their increased free radical scavenging activities. Based on the chemical and DPPH radical scavenging activity changes of $Rg_1$-glycine and $Rb_1$-glycine mixtures by heat-processing, we clearly identified that the increased free radical scavenging activity of ginsenoside is mediated by the Maillard reaction between sugar moiety of ginsenoside and amino acid.

• 농업과학연구
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• 제45권4호
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• pp.761-767
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• 2018

Degenerative diseases are commonly associated with excess free radicals. Acer okamotoanum, a plant endemic to Korea, is reported to have anti-oxidant, anti-cancer, and anti-viral activities. We previously isolated flavonoids from the ethyl acetate fraction of A. okamotoanum such as quercitrin (QU), isoquercitrin (IQ), and afzelin (AF). In the present study, the in vitro antioxidant activity of flavonoids such as QU, IQ, and AF isolated from the ethyl acetate fraction of A. okamotoanum were investigated by measuring the free radical scavenging activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical ($^{\cdot}OH$), and superoxide anion ($O_2{^-}$). The flavonoids (QU, IQ, and AF) concentration-dependently showed a DPPH radical scavenging activity. In particular, QU and IQ showed a higher DPPH radical scavenging activity than that of AF. In addition, the flavonoids (QU, IQ, and AF) at $10{\mu}g/mL$ showed over an 80% scavenging effect against $^{\cdot}OH$ radical production. Furthermore, the $O_2{^-}$ radical scavenging activity of the flavonoids, QU, IQ, and AF increased in a dose-dependent manner. Particularly, IQ exerted the strongest scavenging activities against $^{\cdot}OH$ and $O_2{^-}$ radicals among the other flavonoids. These results indicate that the flavonoids from A. okamotoanum, in particular IQ, would have a protective activity against oxidative stress induced by free radicals, and potentially be considered as a natural antioxidant agent.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.631-642
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• 2017

식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수 분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 6가지 프로티아제를 이용하여 오계란 단백질 가수분해물을 생산하고, 생산된 펩타이드의 항산화 능력을 평가하였다. 그 결과 가수분해도의 최대값은 protamex(46.3%)이고, DPPH 라디칼 소거능 최대값은 bromelain(57.23%), 하이드록시 라디칼 소거능 최대값은 alcalase(30.21%), 슈퍼옥사이드 라디칼 소거능 최대값은 alcalase(58.07%), $Fe^{2+}$ 킬레이션 능력 최대값은 alcalase(72.06%)로 나타났다. 더 나아가 효소별 항산화 저해 능력 $IC_{50}$ 평가하였다. 그 결과 alcalase에 의한 최대값은 금속 킬레이션 능력($IC_{50}$, 1.24 mg/mL)이고, bromelain에 의한 최대값은 DPPH 소거능($IC_{50}$, 2.46 mg/mL)이고, flavourzyme에 의한 최대값은 금속 킬레이션 능력($IC_{50}$, 1.25 mg/mL)이고, neutrase에 의한 최대값은 DPPH 소거능($IC_{50}$, 3.64 mg/mL)이고, papain에 의한 최대값은 DPPH 소거능($IC_{50}$, 3.82 mg/mL)이고, protamex에 의한 최대값은 DPPH 소거능($IC_{50}$, 1.93 mg/mL)이었다. 따라서 protease를 이용하여 오계란 단백질에서 추출한 펩타이드는 항산화 기능성 식품소재로서 활용할 가치가 높을 것으로 기대한다.

• 한국식품영양학회지
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• 제35권5호
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• 한국작물학회지
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• 제50권5호
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• pp.336-339
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• 2005

Common thistle contains water-soluble substances that are antioxidative to foods. Antioxidant activities measured by DPPH method for the ground samples were the greatest in leaves, although was less than that of commonly used antioxidants, BHT and ascorbic acid. Methanol extracts and fractions from Xanthium occidentale plants dose-dependently increased DPPH free radical scavenging activity, in vitro test. The extracts from leaves showed the strongest antioxidant activity. DPPH scavenging activity of the individual fraction was in order of n-butanol>water>ethyl acetate>n-hexane fraction. By means of HPLC analysis, leaf samples of Xanthium occidentale had the highest amount of phenolic compounds, related with antioxidant activity, and followed by stems and roots. Total content of these antioxidant phenolic com­pounds for leaves extracts were detected in water fraction (36.7 mg 100 $g^{-1}$) as the greatest amount, especially chlorogenic acid (39.4 mg 100 $g^{-1}$) was the greatest component. These results suggest that Xanthium occidentale plants had potent antioxidant activity, and their activities were differently exhibited depending on plant part and fraction.

• Natural Product Sciences
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• 제7권1호
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• pp.1-4
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• 2001

The antioxidant activity of Pinus densiflora was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The methanol extract of P. densiflora showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate>n-butanol>water>dichloromethane> n-hexane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further purified by repeated silica gel column chromatography. Antioxidant (+)-catechin was isolated as one of the active principles from the ethyl acetate fraction, together with the inactive components, dihydrokaempferol and 1-O-benzoylglucose. The radical scavenging effect of (+)-catechin on DPPH radical exceeded to that of L-ascorbic acid which is a well known antioxidant.