• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• v.16 no.1
• /
• pp.51-64
• /
• 2012

This paper provides an efficient dimension reduction algorithm of the positive realization of discrete phase type(DPH) distributions. The relationship between the representation of DPH distributions and the positive realization of the positive system is explained. The dimension of the positive realization of a discrete phase-type realization may be larger than its McMillan degree of probability generating functions. The positive realization with sufficient large dimension bound can be obtained easily but generally, the minimal positive realization problem is not solved yet. We propose an efficient dimension reduction algorithm to make the positive realization with tighter upper bound from a given probability generating functions in terms of convex cone problem and linear programming.

• Archives of Pharmacal Research
• /
• v.16 no.2
• /
• pp.118-122
• /
• 1993

The effects of n-alkanols on the rotational relaxation time of 1, 6-dipheny-1, 3, 5-hexatriene (DPH) in synaptosomal plasma membrane vesicles isolate from fresh bovine cerbral contex were investigated. n-Alknols decreased the rotational relaxation time of 1, 6-diphenyl-1, 3, 5-hexatriene in the native membranes and the potencies of n-alkanols up to 1-nonanol increased by 1 order of magnitude as the carbon chain length increases by two carbon atoms, The cut-off phenomenon was reached at 1-decanol, where further increase in hydocabon length resulted in an increase in the rotational relaxation time of DPH in the native membranes.

• Archives of Pharmacal Research
• /
• v.26 no.8
• /
• pp.597-600
• /
• 2003

Silybin has a potent antibacterial activity, more potent than silymarin II, against gram-positive bacteria without hemolytic activity, whereas it has no antimicrobial activity against gram-negative bacteria or fungi. The mode of action of silybin against the gram-positive bacterial cell was examined by investigating the change in plasma membrane dynamics of bacterial cells using 1 ,6-diphenyl-1,3,5-hextriene (DPH) as a membrane probe and by assessing the inhibition of macromolecular synthesis using radiolabeled incorporation assay. The results showed that silybin inhibited RNA and protein synthesis on gram-positive bacteria.

• Development and Reproduction
• /
• v.20 no.4
• /
• pp.275-281
• /
• 2016

The purpose of this study was to investigate the improvement of growth in Israeli carp (Cyprinus carpio), and the cross experiment was carried out with two strains of Israeli carp. Four combinations of Israeli carp from Jeonbuk fisheries farm and Songpu mirror carp from Heilong Jiang, China (KK; Jeonbuk ♀ ${\times}$ Jeonbuk ♂, KC; Jeonbuk ♀ ${\times}$ China ♂, CC; China ♀ ${\times}$ China ♂ and CK; China ♀ ${\times}$ Jeonbuk ♂) were developed and reared. Body length, body weight and condition factor were determined at 20, 40, 60 and 170 days post-hatch (DPH). The results showed that there were differences in growth rate of the four groups. Body length of four groups were CK > CC > KC > KK and body weight were CC > CK > KC > KK at 170 DPH. The growth perfomance of four groups were statistically significant difference (P<0.05). During the rearing, CC group had longer length and higher weight at 170 DPH compared to other three groups and also condition factor was highest in the CC group, but there was no significant difference in a survival rate. These results indicated that the growth performance mainly depended upon brooder combination but survival rate could not significantly affect brooder.

• Journal of Environmental Health Sciences
• /
• v.26 no.2
• /
• pp.14-21
• /
• 2000

DEAE-Toyopearl 650S, Superdex pg-75, Poros HQ, Superdex H200의 각종 칼러크로마토그래피를 이용하여 C.bifermentans DPH-1균주로부터 테트라클로로에틸렌(PCE) 분해 효소를 정제했다. 이 PCE 분해효소 (PCE dehalogenase)는 PCE를 환원적 탈염소화 반응에 의해 시스디클로로에딜렌 (cis-1,2-dichloroethylene)에 전환 가능하여, 이 때의 Vmax 및 Km 치는 각각 73 nmol/h.mg protein, 12$\mu$M이었다. 본 PCE dehalogenase의 겔여과 분자량 Maker Kit를 이용한 분석결과(70kDa)는 SDS-PAGE에 나타난 분자량(35kDa)의 약 2배인 것으로 확인되었다. 따라서 본 효소는 분자량 70kDa의 이량체(Homo dimer)인 것으로 추정되었다. 본 효소의 최적온도 및 pH는 각각 35$^{\circ}C$ 및 8.0 이었다. 또한 본 효소는 PCE외의 트리클로로에틸렌, 디클로로에틸렌 이성체, 1,2-디클로로에템, 1,2-디클로로프로판, 1,1,2-트리클로로에탄에 대하여도 활성을 타나내었다. N-말단 아미노산 분석결과에서도 본 효소는 현재 알려진 PCE dehalogenase와 그 배열이 전혀 다른 것으로 나타나 각종 유기염소 화합물의 분해능을 보유한 신종의 PCE 분해효소인 것이 확인되었다.

• Archives of Pharmacal Research
• /
• v.15 no.3
• /
• pp.196-203
• /
• 1992

Intramolecular excimer formation of 1, 3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1, 6-diphenyl-1, 3, 5-hexatriene (DPH) were used to investigate the effects of barbiturates on the fluidity of model membranes of phosphatidycholine (SPMVPC), phosphatidylserine (SPMVPS), and phosphatidylinositol (SPMVPI) fractions of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex. In a dose-dependent manner, barbiturates decreased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py and increased the anisotropy(r), rotational relaxation time (P), limiting anisotropy $(r_infty)$, and order parameter (S) of DPH in SPMVPC, SPMVPS and SPMVPI. This indicates that barbiturates decreased both the lateral and rotational diffusion of the probes in SPMVPC, SPMVPS and SPMVPI. The relative potencies of barbiturates in ordering the membranes were in the order: pentobarbital > hexobarbital > amobarbital > phenobarbital. This order correlates well with the anesthetic potencies of barbiturates and the potencies for enhancement of $\gamma$-aminobutyric acid-stimulated chloride uptake. Thus, it is strongly suggested that a close relationship might exist between the membrane ordering effects of barbiturates and the chloride fluxes across SPMV.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
• /
• v.5B no.2
• /
• pp.181-188
• /
• 2005

In this paper, a DC smoothing filterless soft switching pulse modulated high frequency AC power conversion circuit connected to utility. frequency AC power source is proposed for consumer induction heating hot water producer, steamer and super heated steamer. The operating principle of DC link filterless utility frequency AC-high frequency AC (HF AC) power conversion circuit defined as high frequency cycloinverter is described, which can operate under a principle of ZVS/AVT and power regulation based on alternate asymmetrical PWM in synchronization with the utility frequency single phase AC positive or negative half wave voltage. The dual mode modulation control scheme based on high frequency PWM and commercial frequency AC voltage PDM for the proposed high frequency cycloinverter are discussed to enlarge its soft switching commutation operating range for wide HF AC power regulation. This high frequency cycloinverter is developed for high frequency IH Dual Packs Heater (DPH) type boiler used in consumer and industrial fluid pipeline systems. Based on the experiment and simulation results, this high frequency cycloinverter is proved to be suitable for the consumer use IH-DPH boiler and hot water producers. The cycloinverter power regulation and power conversion efficiency characteristics are evaluated and discussed.

• Journal of Environmental Health Sciences
• /
• v.26 no.2
• /
• pp.6-13
• /
• 2000

PCE(tetrachloroethylene) 분해능을 보유한 그람 양성, 내생포자 형성의 혐기성균이 일본 기후현의 한 전자제품공장으로부터 분리되었다. 이 균은 생화학적 특성 및 16S rRNA 분석결과에 의하여 C. bifermentans인 것을 거쳐 cDCE(cis-1,2-dichloroethylene)로 전환되었다. 전자공여체로서 효모엑기스는 PCE 분해에 있어 가장 효과적이었으며 효모엑기스를 공급한 조건에서의 PCE 탈염소화 속도는 0.41 $\mu$mol/h.mg protein 이었다. 한편 본 균주는 PCE 뿐만 아니라 각종 유기염소화합물에 대해서도 분해능을 보유하고 있는 신종의 PCE 분해균으로서 각종 유기염소화합물에 오염된 지하수 및 토양에서의 In situ bioremediation 적용에 있어 유용할 것으로 기대된다.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.7 no.1
• /
• pp.48-54
• /
• 2002

This paper is described the indirect induction heated boiler and induction heated hot air producer using the voltage-fed series resonant high-frequency inverter which can operate in the frequency range from 20 kHz to 50 kHz. A specially designed induction heater is composed of laminated stainless plates, which have many tiny holes and are interconnected by spot welding. This heater is inserted into the ceramic type vessel with external working coil. This working coil is connected to the inverter and turbulence fluid through this induction heater to moving fluid generates in the vessel. The operating performances of this unique appliance in next generation and its effectiveness are evaluated and discussed from a practical point of view.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.12
• /
• pp.1827-1834
• /
• 2020

Candida albicans is a major fungal pathogen in humans. In our previous study, we reported that an ethanol extract from Aucklandia lappa weakens C. albicans cell wall by inhibiting synthesis or assembly of both (1,3)-β-D-glucan polymers and chitin. In the current study, we found that the extract is involved in permeabilization of C. albicans cell membranes. While uptake of ethidium bromide (EtBr) was 3.0% in control cells, it increased to 7.4% for 30 min in the presence of the A. lappa ethanol extract at its minimal inhibitory concentration (MIC), 0.78 mg/ml, compared to uptake by heat-killed cells. Besides, leakage of DNA and proteins was observed in A. lappa-treated C. albicans cells. The increased uptake of EtBr and leakage of cellular materials suggest that A. lappa ethanol extract induced functional changes in C. albicans cell membranes. Incorporation of diphenylhexatriene (DPH) into membranes in the A. lappa-treated C. albicans cells at its MIC decreased to 84.8%, after 60 min of incubation, compared with that of the controls, indicate that there was a change in membrane dynamics. Moreover, the anticandidal effect of the A. lappa ethanol extract was enhanced at a growth temperature of 40℃ compared to that at 35℃. The above data suggest that the antifungal activity of the A. lappa ethanol extract against C. albicans is associated with synergistic action of membrane permeabilization due to changes in membrane dynamics and cell wall damage caused by reduced formation of (1,3)-β-D-glucan and chitin.