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Application of automatic dry chemistry analyzer (FUJI DRI-CHEM 3000) used to hematological analysis of cultured freshwater fish in low temperature season (담수산 양식어류의 혈액검사에 사용된 건식 자동생화학 분석기 (FUJI DRI-CHEM 3000) 의 활용 가능성)

  • Jung, Sung-Hee;Seo, Jung-Soo;Kim, Jin-Do;Choi, Hye-Sung;Park, Myoung-Ae
    • Journal of fish pathology
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    • v.24 no.3
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    • pp.247-254
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    • 2011
  • The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in cultured freshwater fish and also evaluate application of automatic dry-type chemistry analyzer (FUJI DRI-CHEM 3000) used to those blood tests. A blood profile of total 200 fish for rainbow trout (Onchorhynchus mykiss), israel carp (Cyprinus carpio), tilapia (Oreochromis niloticus) and eel (Anguilla japonica) cultured in Inland Fisheries Research Institute of NFRDI was determined by hemoglobin (Hb) and plasma chemistry tests: total protein (TP), albumin (ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), triglyceride (TG), total cholesterol (TCHO), creatinine (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose (GLU). The values of ALT, TG, LDH, ALB, TCHO, AST and ALP were outside from the minimum and/or maximum of the established detectable range of the analyzer. ALT and TG were not detectable in the range of 67%~61.5%. LDH, ALB and TCHO were not detectable in the range of 36~17%. AST and ALP were not detectable in the range of 5.5~0.5%. However, the values of BUN, CRE, GLU, Hb and TP were below the detectable limits of the analyzer.

Study on the Safety of Playground Flooring Made of Polyolefin Foam Waste and Rubber Paving (폐폴리올레핀 폼과 탄성 포장재로 구성된 어린이 놀이터 바닥의 안전성에 관한 실험적 연구)

  • Choi, Soo-Kyung;Jun, Myoung-Hoon;Lee, Do-Heun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.1 no.3
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    • pp.246-254
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    • 2013
  • This study is purposed to verify the safety of the floor of the children's playground using polyolefin foam waste and rubber paving. The critical fall height, walking suitability, long term physical fatigue expectation and slipperiness were tested. Polyolefin foam wastes in thickness of 30mm, 50mm and 70mm were prepared with paving the rubber paving materials in 12mm and 15mm thickness respectively. The test on the critical fall height was carried out according to KS G 5758:2009. The floor hardness test equipment (O-Y HMA) was used for the test on hardness of the floor from a viewpoint of walking suitability and fatigue. A portable slipperiness tester (ONO PPSM) was used for slipperiness test. It was revealed from the test that the floor made of the polyolefin foam waste and rubber paving were considerably safe from a viewpoint of impact absorption. With regards to the hardness of the floor, it was shown the excellent performance in the aspects of walking and fatigue for male. But it was not suitable with walking on the shoes(middle heels) for female. And they will be very fatigue if they were in a long time walking or standing. As far as the slipperiness is concerned, it was shown that it was comparatively safe for the ordinary motions even though the surface was wet.

Monitoring of Residual Pesticides in Commercial Agricultural Products (유통중인 농산물의 잔류농약 모니터링)

  • Nam, Hye-Seon;Choi, Yong-Hoon;Yoon, Sang-Hyeon;Hong, Hye-Mi;Park, Yong-Chjun;Lee, Jin-Ha;Kang, Yun-Sook;Lee, Jong-Ok;Ahn, Yeong-Sun;Hong, Yeong-Pyo;Kim, Hee-Yun
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.317-324
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    • 2006
  • Pesticide residues were monitored in agricultural products purchased at Gyeongin and Jeolla-do provinces. Total 245 pesticides were analysed by GC-MS and LC-MS/MS, and residual pesticides were detected in 70 samples (19.7%), with spinach and chard showing high detection frequency of 35%. Fifteen samples (4.2%) exceeded Korea Maximum Residue Limits (MRLs), and 30 types of pesticides were detected in samples with azoxystrobin, procymidine, endosulfan showing considerably high frequencies in respectively 14, 13, and 13 samples. The amount of 11 pesticide including chlorpyrifos, indoxacarb among them were detected over Korea MRLs. No residual pesticides were detected in 285 (80.3%) samples, and residual pesticides levels of 55 samples (15.5%) were lower than Korea MRLs, indicating 95.8% samples were relatively safe.

A Study on Morphology Measurement and Comparison of Nutria(Myocastor coypus) Inhabiting in Korea (국내 서식하는 뉴트리아(Myocastor coypus)의 형태측정 및 비교에 관한 연구)

  • Lee, Do-Hun;Kil, Jihyon
    • Journal of Environmental Impact Assessment
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    • v.22 no.3
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    • pp.241-254
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    • 2013
  • The nutria(Myocastor coypus) that is originated from South America is a representative Invasive alien species in Korea. invasive alien species is one of the biggest causes for the loss of biodiversity and it may threaten the conservation and function of ecology as well as the welfare of mankind. Intense habitation has been made around the Nakdong River basin area that this study has analyzed the habitat density of nutria for the subject of 3 survey sites in the Nakdong River basin area. As a result of survey on habitation density in 2011 and 2012, under St.1 it was shown $2.01{\pm}0.21$/ha in 2011 and $0.69{\pm}0.55$/ha in 2012. Under St.2, it was shown $0.91{\pm}0.37$/ha in 2011 and $0.55{\pm}0.39$/ha in 2012. Under St.3, it was shown $5.31{\pm}0.72$/ha in 2011 and $3.99{\pm}2.31$/ha in 2012. The wet survey areas of St.1 and St.3 had shown relatively high habitation density compared to St. 2, the river survey area, and the average annual habitation density of the survey area was $2.74{\pm}2.29$/ha in 2011 and $1.74{\pm}1.18$/ha in 2012 that it has shown slightly reducing trend. As a result of measuring the morphology by capturing the habitation individuals, the average body length of adults is $92.23{\pm}9.41cm$, the length from the head to body for $53.90{\pm}5.15cm$, tail length for $38.33{\pm}4.83cm$, hind foot length for $13.82{\pm}1.00cm$, front foot length for $6.02{\pm}0.56cm$, and weight for $5.48{\pm}1.08kg$. As a result of comparing the types between genders, male showed a slightly higher figure in all parts compared to female and it showed significantly difference in total body length, head-body length, front foot length and total weight. As a result of analysis for each head-body length, other measured parts, weight and relativity for each measured part, all bodily part is shown to have the higher volume of correlations. The condition index of individuals in the survey area was shown in the average of $35.67{\pm}4.78$ with female for an average of $36.60{\pm}5.19$ and male for an average of $34.73{\pm}4.34$. The winter temperature in the southern area of Korea is considered for not greatly impacting on the habitation and development of nutria that, if there is no artificial control, it is considered to have certain concern of showing drastic breeding and territory expansion for the habitation group.

Contents analysis of the community dental hygiene practice outcome evaluation (지역사회치위생학 현장(보건소)실습 성과평가 내용분석)

  • Lee, Ga-Yeong;Han, Yang-Keum;Kim, Young-Kyung;Lim, Hyun-Ju;Kown, Yang-Ok;Kim, Han-Mi;Park, Jeong-Ran;Kim, Nam-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.16 no.1
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    • pp.37-43
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    • 2016
  • Objectives: The purpose of the study is to evaluate the contents analysis of the community dental hygiene practice in the dental hygiene students and suggest the outcome based evaluation index of community dental hygiene practice. This study will provide the basic data for the community nursing and social welfare practice. Methods: A self-reported questionnaire was completed by 82 dental hygiene professors and 254 dental hygienists in community health centers after receiving informed consent based on institutional review board from 5th Dec 2014 to 30th Jul 2015. A total of 49 professors and 134 dental hygienists gave the complete answers. The study instrument was adapted from the literature review. The questionnaire consisted of evaluation, purpose of evaluation, and evaluation format. Data were analyzed using SPSS 20.0 program. The contents analysis was carried out through input, process, output, and outcome. Results: The practice outcome evaluation was conducted in 62.9% of the dental hygiene departments and 32.9% of the community health centers. Most of the dental hygiene professors chose "To know what students learned(27.3%)" and "To ensure student's role and behavior in practice(27.3%)." as the purpose of evaluation. The public dental hygienists chose "To score the practice grade(42.1%)." The evaluation method was done anonymously. Conclusions: The outcome based evaluation in community dental hygiene practice was the best method to evaluate the practice education for the competency of the dental hygiene students.

Detection of Antibodies Reacting with Anaplasma phagocytophilum and Ehrlichia chaffeensis from Cats, Horses and Cattle in Korea (한국에서 사육되는 고양이, 말, 소로부터 Anaplasma phagocytophilum과 Ehrlichia chaffeensis에 대한 항체 검출)

  • Chae, Joon-Seok;Heo, Eun-Jeong;Park, Jin-Ho;Choi, Kyoung-Seong;Dumler, J. Stephen;Lee, Sung-Soo;Kang, Tae-Young;Yang, Jae-Hyuk;Kim, Do-Young;Kim, Joon-Gyu;Choi, Gui-Cheol;Kang, Mun-Il
    • Journal of Veterinary Clinics
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    • v.26 no.6
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    • pp.515-519
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    • 2009
  • Antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected by the immunofluorescent antibody (IFA) test in sera collected from cats, thoroughbred horses and Holstein cattle in Gwangju, Jeonju and Jeju Island of Korea. Two hundred fifty four sera (33 feral and pet cats, 92 grazing horses and 129 grazing cattle) were obtained from Republic of Korea. Antibodies to A. phagocytophilum (titer $\geq$ 80) were detected in 6 of the 33 feral and pet cats (18.2%), and 1 seropositive cat (3.0%) also had antibodies to E. chaffeensis. Only 1 of 129 (0.8%) cattle and 2 of 92 (2.2%) horses had antibodies to A. phagocytophilum. Antibodies to E. chaffeensis were not detected in either of these animals. This is the first report of serological evidence of A. phagocytophilum and E. chaffeensis from cats, cattle and horses in Korea. These rickettsial agents could have an important impact on human health or impact animal health with economic losses among industrial grazing animals in Korea.

Identification of Secretion Signals of Target Proteins in Salmonella enterica serovar Typhimurium and Construction of Secretion Vector using this Signal (Salmonella enterica serovar Typhimurium에서 Type III 분비장치의 표적단백질들의 분비신호 확인 및 Type III 분비장치를 이용한 Secretion Vector의 개발)

  • Choi, Hyuk-Jin;Eom, Joon-Ho;Cho, Jung-Ah;Lee, Sun;Lee, Kyoung-Mi;Lee, In-Soo;Park, Yong-Keun
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.254-258
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    • 2000
  • Invasion process of bacterial cell into intestinal epithelium is important in Salmonella infection. The invasion is induced by the proteins secreted by type III secretion appratus of Salmonella. It has been known that the proteins do not have N-terminal signal peptide existing in general secreted proteins. Recent studies on Yersinia reported that secretion signal of type III appratus may lie on 5'end secondary structure of mRNA of secreted protein. In this study, we constructed translational fusion of ompR and sopE, encoding type III secretion protein of Salmonella, and observed secretion of the fusion protein for investigating the secretion signal of Salmonella type III appratus. The sopE DNA fragments of the translational fusion contain the region of promoter and from start code to tenth or to fifth code. These translational fusions indicate that type III secretion signal of Salmonella is located on 5'end of mRNA encoding secreted protein. We constructed prototype of secretion vector using this signal to produce useful foreign protein.

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Effects of In-depth Science Learning Through Multiple Intelligence Activities on the Science Inquiry Abilities and Interests of Elementary School Children (초등학교 과학과 심화학습에서 다중지능을 활용한 과학활동이 초등학생의 과학탐구능력과 흥미에 미치는 효과)

  • 이영아;임채성
    • Journal of Korean Elementary Science Education
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    • v.20 no.2
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    • pp.239-254
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    • 2001
  • The in-depth learning course newly established in the 7th National Curriculum of Science is for students who have mastered regular subject matters on a science topic and want to learn it more deeply or by different ways. Individual learners have their own unique intellectual properties. The study examined the effects of in-depth science learning using multiple intelligence activities on the science inquiry abilities and interests of elementary school children. This study involved two fifth-grade science classes in Busan. Each class was assigned to comparison and experimental group. The science topics covered during the period of the study were Units of Matter and Earth. After studying each regular content formulated by the National Curriculum, the students of comparison group experienced traditional practices of in-depth science, whereas those of experimental one performed the Multiple Intelligence(MI) activities related to the content. Students of both groups were pre- and posttested using the inventories of Science Inquiry Ability and Science Interest. Also, after instruction on the topics, students were interviewed to collect more information related to their loaming. The results are as follows. First, the science inquiry abilities of children were increased by using activities based on MI during the in-depth science teaming. Two inquiry processes, that is, the Prediction which is regarded as one of the basic process skills in science and the Generalization regarded as one of integrated process skills showed statistically significant differences between the groups, although the differences of other skills not significant but more improvements in experimental group than comparison one. Second, the in-depth science loaming through MI contributed to the increasing of interests of the children in science. The scores on Science Interest measured in pretest and posttest with the two groups showed st statistically significant difference. For interest in science instruction, children of experimental group showed high level of interest for the various MI activities, and, although the comparison groups' level of the interest was low, they revealed that they want to experience the MI activities in future instruction of science. Interviews with the children randomly selected from the experimental group when they completed the in-depth programs showed that most of them had much interest in MI activities. Especially, they attributed significant meanings to the experiences of teaming with their friends and doing activities that they want to do. These findings have important implications about usefulness of MI in science instruction. The results also highlight the need for science teachers to provide a variety of experiences and to create environments which encourage the children to use MI to learn a science topic.

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A Meta Analysis and Review of External Constructs Based on the Technology Acceptance Model: Focusing on the Research Related of Smartphone in Korea (기술수용모델 관련 외부요인에 관한 문헌적 고찰 및 메타분석: 우리나라 학회지에 게재된 스마트폰 관련연구 중심으로)

  • Nam, Soo-tai;Kim, Do-goan;Jin, Chan-yong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.254-257
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    • 2013
  • A Meta-analysis is a statistical literature synthesis method that provides the opportunity to view the research context by combining and analyzing the quantitative results of many empirical studies. The Technology Acceptance Model (TAM) has been the subjects of a great deal fields and now also has been continuously studied. Recently, the issue of the biggest the information communication and information systems fields is smart. At this point, we conducted a Meta-analysis and review of external constructs based on the technology acceptance model. This study was targeted a total of 106 research papers that are setting up the causal relationship in the technology acceptance model among the research papers published in domestic academic journals before 2013. The result of the Meta-analysis, the biggest effect size the path from external Variables to perceived usefulness was showed playfulness. The effect size was 0.536 in the path from perceived usefulness to playfulness. And, the biggest effect size the path from external Variables to perceived ease of use was showed self efficacy. The effect size was 0.536 in the path from perceived ease of use to self efficacy. Based on the results, it was discussed the difference through comparative analysis with previous research.

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Prevalence and Correlates of Physical Activity and Sitting Time in Cancer Survivors: 2009-2013 Korea National Health and Nutrition Examination Survey

  • Kim, Byung Hoon;Lee, Hyo
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.12
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    • pp.5295-5302
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    • 2016
  • Objectives: A physically active lifestyle is important for cancer survivors. Therefore, this study was conducted to 1) provide population-based estimates of the prevalence of physical activity and sitting time, and 2) their correlates in Korean cancer survivors. Materials and Methods: This study analyzed a cancer survivor subsample (N=1,482) from 2008-2013 Korea National Health and Nutrition Examination Survey (KNHANES), data selected with a complex sampling design. Overall and subgroup-specific prevalences of physical activity and sitting time were estimated. Correlates of moderate- to vigorous-intensity physical activity ( MVPA) and sitting time were tested using age-group-specific hierarchical multiple regression models. Results: Overall adherence rate to physical activity guidelines was 34.9% (95% CI=31.5-38.4). Age-group-specific adherence rates were 41.1% (95% CI=36.3-45.9) in adults (30-64 years old), and 25.3% (95% CI=21.0-25.3) in older adults (65 years or older). Adults spent 213.33 minutes (95% CI=172.4-254.3) per week on MVPA and 55.3 minutes (95% CI=36.4-64.6) on sitting time per day. In adults, sitting time was significantly associated with employed status (B=28.0, p=0.046), smoking (B=-47.4, p=0.020), and number of comorbidity conditions (B=-13, p=.037). MVPA was significantly associated with marital status (B=134.9, p<0.001), employment status (B=98.12, p=.046), and years since cancer diagnosis (B=104.7, p=0.015). Older adults spent 162.2 minutes (95% CI=119.5-204.8) per week on MVPA and 63.0 minutes (95% CI=45.0-89.5) on sitting time per day. Their significant correlates were sex (B= -45.2, p=0.014), smoking (B=-70.14, p<0.001), and years since cancer diagnosis (B=37.0, p=0.024). Age (B=5.8, p=0.042) and marital status (B=83.8, p=0.033) were also significantly associated with MVPA in older adults. Conclusion: A majority of Korean cancer survivors do not sufficiently participate in physical activity. In general, older, unhealthier, non-working, and being unmarried were risk factors for physical inactivity. While this study informs public health policy makers and practitioners about physical activity intervention demand for cancer survivors, future investigations should address psychosocial mediators to better inform intervention programs.