• Title/Summary/Keyword: DO-254

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Metagenomic Analysis of Jang Using Next-generation Sequencing: A ComparativeMicrobial Study of Korean Traditional Fermented Soybean Foods (차세대 염기서열 분석을 활용한 장류의 메타지놈 분석 : 한국 전통 콩 발효식품에 대한 미생물 비교 연구)

  • Ranhee Lee;Gwangsu Ha;Ho Jin Jeong;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
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    • v.34 no.4
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    • pp.254-263
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    • 2024
  • Korean jang is a food made using fermented soybeans, and the typical products include gochujang (GO), doenjang (DO), cheonggukjang (CH), and ganjang (GA). In this study, 16S rRNA metagenome analysis was performed on a total of 200 types of GO, DO, CH, and GA using next-generation sequencing to analyze the microbial community of fermented soybean foods and compare taxonomic (biomarker) differences. Alpha diversity analysis showed that in the CHAO index, the species richness index tended to be significantly higher compared to the DO and GA groups (p<0.001). The results of the microbial distribution analysis of the GO, DO, CH, and GA products showed that at the order level, Bacillales was the most abundant in the GO, DO, and CH groups, but Lactobacillales was most abundant in the GA group. Linear discriminant analysis effect (LEfSe) analysis was used to identify biomarkers at the family and species levels. Leuconostocaceae, Thermoactinomycetaceae, Bacillaceae, and Enterococcaceae appeared as biomarkers at the family level, and Bacillus subtilis, Kroppenstedtia sanguinis, Bacillus licheniformis, and Tetragenococcus halophilus appeared at the species level. Permutational multivariate analysis of variance (PERMANOVA) analysis showed that there was a significant difference in the microbial community structure of the GO, DO, CH, and GA groups (p=0.001), and the microbial community structure of the GA group showed the greatest difference. This study clarified the correlation between the characteristics of Korean fermented foods and microbial community distribution, enhancing knowledge of microorganisms participating in the fermentation process. These results could be leveraged to improve the quality of fermented soybean foods.

Middle School Students' Ideas about the purposes of Laboratory Work (과학 실험의 목적에 대한 중학생의 인식조사)

  • Kim, Hee-Kyong;Song, Jin-Woong
    • Journal of The Korean Association For Science Education
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    • v.23 no.3
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    • pp.254-264
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    • 2003
  • Researches on laboratory work show that students often achieve little meaningful learning through laboratory work. One reason for this failure is that students often do not know the different types of laboratory work and the 'purposes' of them. Therefore, this study investigated middle school student' ideas about the purposes of laboratory work. To seventh grade students(n=147) of middle school in Seoul, Korea, we asked (Question 1) "Why do scientists do laboratory work?" and (Question 2) "Why do you do laboratory work in science classes?" It was required a short essay including the reasons and examples of them. From the results, it was found that 56.8% of the students had ideas that scientists do laboratory work for discovering new facts or inventing something, and 82.9% of the students responded that they do laboratory work for understanding and memorizing the contents of science textbook. In addition, the differences according to gender and to school achievement level, and the relationship between the ideas about scientists' laboratory work and about school science laboratory work were examined. The results showed that boys responded 'social usefulness' more frequently than girl, while girls mentioned 'personal pleasure' more frequently than boys in relation to the purposes of scientists' laboratory work(p<.05). According to the achievement level, it was founded that 'middle' level students replied 'to remember' more frequently than high and low levels in relation to school science laboratory work. Finally, students who had ideas that scientists do laboratory work for verifying a theory had the similar ideas about school science laboratory work. In conclusion, students are lack of diverse and proper views about laboratory work. It is recommended that teacher need to make clear the purpose of laboratory work and help students to understand of it.

Applicable Feasibility of Fenton Oxidation and Zeolite Ion Exchange Processes for Removal of Non-Biodegradable Matters and Ammonia in Livestock Wastewater (축산폐수 중의 난분해성 물질 및 암모니아 제거를 위한 Fenton 산화와 Zeolite 이온교환 공정의 적용 가능성)

  • Cho, Chang-Woo;Kim, Byoung-Young;Chae, Soo-Choen;Chung, Paul-Gene
    • Journal of Korean Society on Water Environment
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    • v.23 no.5
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    • pp.600-606
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    • 2007
  • Livestock wastewater containing concentrated organic matters and nutrients has been known as one of the major pollutants. It is difficult to apply the conventional activated sludge process to treat livestock wastewater because of high Non-biodegradable (NBD) matter and ammonia. The objectives of this study are to remove NBD matters including aromatic compounds and ammonia in livestock wastewater using Coagulation-Fenton oxidation-Zeolite (CFZ) processes and ascertain applicable feasibility in the field through pilot plant experiment. NBD matters and color remained in the treated water were removed over 92% by Fenton oxidation as the second treatment process. Ammonia was removed by over 99.5% in the zeolite ion exchange process as the last treatment method. From $UV_{254}$, $E_2/E_3$ ratio and GC/MS analyses of treated water at each process, the aromatic compound was converted to aliphatic and aromaticity was decreased. In pilot scale test, organics and ammonia removal efficiencies were not much different from the result of lab-scale test at various operation conditions. Furthermore, reaction time and dosage of Fenton reagent in pilot scale experiment reduced by 40 min and 50% rather than in lab-scale test. $BOD_5$, $COD_{Mn}$, SS, T-N and T-P of treated water in the pilot-scale experiment also met the effluent standards.

Occurrence Patterns of Naturalized Plants by Agricultural Types in the Gyeonggi and Chungcheong Provinces, South Korea (농경지 유형에 따른 경기, 충청지역 귀화식물의 출현 양상)

  • Yang, Dongwoo;Lee, Eunjeong;Jeong, Seonah;Woo, Ayoung;Nam, Kijeong;Han, Donguk;Kim, Myung-Hyun;Park, Sangkyu
    • Korean Journal of Ecology and Environment
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    • v.50 no.2
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    • pp.254-265
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    • 2017
  • To elucidate occurrence patterns of naturalized plants in agroecosystems, we surveyed flora in uplands, orchards and rice paddy fields in middle regions (Gyeonggi-do and Chungcheong-do) of Korea in summer and fall from 2013 to 2015. Our results showed that there are 77 species of naturalize plants in 54 genera and 20 families of vascular plants among total 420 species occurred in the agricultural areas composing 18.3% of naturalized index. Community compositions of both native and naturalized plants were different among agricultural types. We suggested that Cerastium glomeratum Thuill., Bidens frondosa L. and Erigeron philadelphicus L. are capable of dispersal to other regions based on our results with getting to broaden distribution of naturalized plants. The mean naturalized index was the highest in the upland (25.1%) followed by in the orchard (23.0%) and the paddy field (16.1%), while the mean number of naturalized species was the highest in the orchard (21.8 species) followed by in the upland (16.6 species) and paddy field (10.3 species). The numbers of naturalized plants in the orchard were increased along with increasing size of the agricultural land and decreasing ecological area rate. In addition, the number of native species was significantly correlated with the number of naturalized species in the upland. Our results would provide useful information to manage dispersion of naturalized plants in agricultural ecosystems.

Efficiency of Activated Carbon Treatment Processing on Raw Water Purification for Nakdong River (활성탄을 이용한 낙동강 상수원수의 수처리 효과)

  • Lim, Young-Sung;Kang, Gwan-Ho;Lee, Hong-Jae;Seo, Dong-Cheol;Heo, Jong-Soo;Sohn, Bo-Kyoon;Cho, Ju-Sik
    • Korean Journal of Environmental Agriculture
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    • v.21 no.3
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    • pp.208-215
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    • 2002
  • This study was carried out to evaluate the pollutant removal efficiencies of the advanced drinking water treatment using activated carbon process. for raw water, Nakdong river was used. from the activated carbon adsorption experiment the fellowing results were obtained The efficiency of water treatment enhanced with increase in empty bed contact time. Variation of pH was not detected to the bed depth, but DO content gradually decreased with the bed depth. Removal efficiency of $KMnO_4$ consumption, UV254 absorption, DOC and THMFP also were increased by increasing in the bed depth. Transition of adsorption zone from upper parts of the bed to the lower parts were detected as treatment periods increased. Large portion of DOCs were degraded and removed by the microbes growing on the surface of activated carbons. Cell numbers of microbes were estimated over $1.1\times10^7\;cell/cm^3$ at the depth of 20 cm from the surface 126 days after starting operation. The results shown that the activated carbon Inter was successfully acted as a biofilm filter.

The Planting and Use of Landscaping Plants in Kangweon-Do (강원도내 조경식물의 배치과 이용)

  • 이기의;이우철;박봉우;조철길
    • Journal of the Korean Institute of Landscape Architecture
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    • v.15 no.3
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    • pp.33-50
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    • 1988
  • This study was executed to find out how to improve on the planting and use of the plants in Kangweon- Do by surveying the planting areas -gardens, parks, streets, schools, etc. - in five cities of this province, and to select available native plants by surveying the main mountains in this province. The results are as follows ; 1. The species number within surrey areas was 319 species. and the species of which planting frequency was very high were Hibiscus syriacus, Juniperus chinensis, Buxus microphylla v. koreana, etc.. 2. The species number of school trees and flowers of 202 schools in Kangweon-Do were 33 species, 32 species respectively. and the species of school trees and flowers that showed the highest preference were Juniperus chinensis, Forsythia koreana each. 3. The species number of flowers and trees designated in 22 City and Keun were 14 species, 7 species respectively, and the species of flowers and trees that presented the highest designation frequency were Rhododendron schlippenbachii, Ginkgo biloba each. 4. The street trees planted along the main streets in Kangweon-Do were 18 species and 84,939 individuals, and the ratio of Populus alba${\times}$glandulosa was the highest among occurrence individuals. 5, As for the composition ratios of life forms of plants within survey areas, the ratio of deciduous broad-leaved tree was the highest as about 56% and that of deciduous coniferous tree was the lowest as about 1.6%. The ratios of native species versus exotic were 43 : 57 6. Through these results, it was thought that the diversification of planing species, the selection of plants suitable to each space and the generalization of use of native species were needed. So 254 plants native to Kangweon-Do were presented to correspond to these requirements.

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Analysis of Elementary School Students' Intake Frequency and Opinions Regarding Native Local Foods in School Foodservice -Focus on Regional Comparison of Gyeongsang-do and Jeolla-do- (향토음식의 학교급식 적용 활성화를 위한 초등학생의 향토음식 섭취 빈도 및 견해 조사 -경상도과 전라도 지역의 비교를 중심으로-)

  • Hwang, Hye-In;Kim, Hyun-Ah
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.2
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    • pp.275-280
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    • 2015
  • This study investigated intake frequency and opinions regarding native local foods in school foodservice and compared eating status between students in Gyeongsang-do and Jeolla-do. Questionnaires were distributed to 300 students at elementary schools located in Gyeongsang-do and Jeolla-do. The results of this study were as follows. First, intake frequencies of Gyeongsang-do-native local foods by students in Gyeongsang-do was significantly higher than compared to students in Jeolla-do (P<0.01). Second, there was no significant difference in concern for native local foods between students in Gyeongsang-do and students in Jeolla-do, although opinions regarding native local foods in school foodservice by students in Gyeongsang-do were higher compared to students in Jeolla-do (P<0.01). In conclusion, there was a regional difference in terms of intake frequency of Gyeongsang-do-native local foods, whereas there was no regional difference in terms of intake frequency of Jeolla-do-native local food. Students in Gyeongsang-do showed more positive attitudes regarding native local foods in school foodservice than students in Jeolla-do. This result implies that regional differences between Gyeongsang-do and Jeolla-do regarding native local foods should be removed. In order to develop native local foods, it will be necessary to develop a program to actively apply native local foods in school foodservice and provide more chances for elementary school students to experience various native local foods.

A Study on Refusal Speech Act of Korean and Thai Learners from a Cross-Cultural Pragmatic Perspective (비교문화적 화용론의 관점에서 본 한국인과 태국인의 거절 화행 연구)

  • Hwang, Sunyoung;Noh, Ahsil;Kunghae, Samawadee
    • Journal of Korean language education
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    • v.29 no.4
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    • pp.225-254
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    • 2018
  • The purpose of this study is to contrast the patterns of realization and understanding of refusal speech acts between Korean and Thai learners. This study intends to answer the following questions: (1) Do Koreans and Thai learners perform refusal speech acts differently? (2) Do Koreans and Thai learners understand refusal speech acts differently? A DCT and a follow-up interview were conducted to collect data of two groups of 30 native Korean speakers and 30 native Thai speakers. For research question 1, we analyzed the refusal strategy and provided reasons given by Koreans and Thai learners depending on the context. For research question 2, we ran a chi-squared test on the elements of the follow-up interviews, such as the weight of burden of refusing, and whether the participant would actually refuse or not. The differences between the refusal strategies of the two groups could be categorized by the preceding inducing speech act. In refusing a request, the difference was prominent in the apologizing strategy, whereas in refusing a suggestion, the difference was mainly in the direct refusal strategy. When refusing an invitation, the most evident difference was the number of refusal strategies employed. When providing an explanation of refusal to people with high social status, Koreans gave more specific reasons for refusals, whereas Thai learners tended to use more vague reasons. Moreover, when refusing an invitation, Koreans primarily mentioned the relationship, and Thai learners showed the spirit of Greng Jai. When asked the weight of burden of refusing, Koreans felt pressured to refuse a request from people with high social status, and a suggestion or invitation from people with high level of intimacy while Thai learners found it highly difficult to make a refusal in all cases. In answering whether they would actually refuse or not, Koreans tried not to make a refusal to people with high level of intimacy, and such a trend was not evident among the Thai. This study can help us better understand the learner's pragmatic failure, and serve as a basis in establishing a curriculum for teaching speech acts.

Evaluation of AFDX Certification Support System by both AFDX Tap and AFDX Analyzer (AFDX Tap과 AFDX 프로토콜 분석기를 이용한 AFDX 네트워크 인증 기술)

  • Park, Pusik;Son, Myeonghwan;Lee, Jeongdo;Yoon, Jongho
    • Journal of Aerospace System Engineering
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    • v.16 no.1
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    • pp.1-11
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    • 2022
  • Avionics Full-DupleX Ethernet (AFDX) is a next-generation avionics network interface technology that is widely applied in the latest aircraft to replace ARINC429 and MIL-STD-1553B. However, the criteria for authenticating an avionics network consisting of AFDX are very scarce. Using AFDX Protocol Analyzer developed by the Korea Electronics Technology Research Institute and AFDX Tap developed by the Korea Aerospace University, we proposed a technology of certification practicality that can verify the normal functioning of avionics equipment with AFDX network interface. Our proposed technology provided the ability to collect precision packets, to verify AFDX specification compliance, and perform automatic tests to reduce the time and cost of authentication of AFDX avionics devices.

Practical Plan and Vascular Plants Around Construct-Reserved Site of Ecological Forest in Baekdudaegan (백두대간 생태숲 조성예정지의 관속식물상과 활용방안)

  • You, Ju-Han;Ra, Jung-Hwa;Cho, Hyun-Ju;Ku, Ji-Na
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.5
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    • pp.42-58
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    • 2009
  • This study was carried out to survey the vascular plants of the construct-reserved site of ecological forest in Kohang-ri, Sangri-myeon, Yecheon-gun, Gyeongsangbuk-do, Korea. The results are as follows. The vascular plants in this site were recorded as 392 taxa; 85 families, 254 genera, 341 species, 45 varieties and 6 forma. The Korean endemic plants were 5 taxa; Salix hallaisanensis, Clematis trichotoma, Chrysosplenium barbatum, Philadelphus schrenckii and Weigela subsessilis. The rare plants designated by Korea Forest Service were 2 taxa; Iris ensata var. spontanea and Aristolochia contorta. The naturalized plants were 25 taxa; Bromus tectorum, Dactylis glomerata, Panicum dichotomiflorum, Bilderdykia dumetora and so forth. In the results of analysis by floristic region, the specific plants were summarized as 31 taxa; Matteuccia struthiopteris, Oxalis obtriangulata, Betula davurica, Cimicifuga heracleifolia and so forth. The plants with approval for delivering oversea were 4 taxa; Salix hallaisanensis, Aristolochia controrta, Clematis trichotoma and Chrysosplenium barbatum. The plant resources were 186 taxa of ornamental plant, 241 taxa of edible plant, 274 taxa of medicinal plant and 202 taxa of others.