• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.488-493
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• 2010

We investigated the effect of Perilla frutescens (PF) ethanol extract powder (PF-E30) on the local allergic reaction activated by anti-DNP IgE and the mast cell-mediated immediate-type allergic reactions induced by compound 48/80 in a mouse model. One gram of PF powder extracted with 30% ethanol at $80^{\circ}C$ contained 12.3 mg of rosmarinic acid. Oral administration of PF-E30 (0.1 to 0.5 mg/kg body weight) significantly reduced plasma histamine levels and inhibited histamine release from peritoneal mast cells in mice activated by compound 48/80 or anti-DNP IgE. Moreover PF-E30 dose-dependently inhibited the production of antigen-induced IgE. These results indicate that the PF ethanol extract inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.

• The Journal of Korean Medicine
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• v.20 no.1 s.37
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• pp.11-21
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• 1999

Dansam, the root of Salvia miltiorrhiza BGE. (Labiatae), has a bitter and a slightly 'cold' property. and is nontoxic. It has been used for the treatment of diseases such as tumors, bruises, gynecologic diseases, menoxenia, anemia and so forth. As an oriental medicine pathway, anal therapy has many kinds of treatments, Retention enema is one the most useful, advantageous methods of anal therapy. This experiment was performed in order to study the effect of an aqueous extract of Salviae radix root(SRRAE) on Immediate type allergic reaction by Retention enema. The results were obtained as follows, 1. SRRAE inhibited compound 48/80-induced Immediate type allergic reaction 100% with the dose of 0.1g/kg by anal treatment. However, SRRAE showed no significant inhibitory effect on the same reaction by oral treatment. When mice were pretreated with SRRAE at a concentration ranging from 0.001 to 1.0g/kg, by intra-anal treatment, the serum histamine levels were reduced in a dose-dependent manner. 2. SRRAE also inhibited by 79.8% local cutaneous allergic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. SRRAE close-dependently inhibited the histamine release from rat peritoneal mast cells(RPMC) by anti-DNP IgE., but SRRAE not inhibited compound 48/80-induced histamine release. 4. SRRAE dose-dependently inhibited tumor necrosis $factor-\;{\alpha}$ $(TNF-\;{\alpha}$) production from RPMC by anti-DNP IgE. Moreover, the level of cAMP in RPMC, when SRRAE was added, significantly increased compared with that of a normal control. According to the above results, anal therapy(Retention enema) of SRRAE may be beneficial in the treatment of systemic and local Immediate type allergic reactions.

• 한국정보통신설비학회:학술대회논문집
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• 2002.08a
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• pp.39-42
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• 2002

RS-232C는 정보기기들을 연결하는 직렬통신 표준으로, 간단한 구조로 인해, 복잡한 요구 조건이 없는 상용 기기들의 제어 및 감시용으로 사용되고 있다. 최근 무선동신기술이 발전함에 따라, 저가격의 무선통신 기술을 활용하여 무선 RS-232C에 대한 요구가 늘어나고 있다. 무선 RS-232C로 기존 유선의 RS-232C를 대체함으로서, 기존 시스템의 변경없이 편리한 접속기능을 대체한 수 있을 것이다. 본 논문에서는 유선 RS-232C를 대체하는 무선 RS-232C 모듈을 채용하여, 전력제어에 사용하는 배전자동화 장치의 관리를 간편하게 하는 시스템의 구현에 대해서 다루고 있다. 배전 자동화시스템은 일종의 SCADA시스템의 일부로서, 통신 프로토콜로서 산업용 DNP3.0을 사용하고 있다. 무선 RS-232C 링크의 신뢰성을 확보하기 위하여, 무선 구간에 DNP3.0을 채택하였으며, 무선 통신방식으로는 무선 RF, 블루투스(Bluetooth), 무선랜(wireless LAN)등 3가지 방식을 채용하여, 설계, 구현내용을 기술하였다.

• Journal of Pharmaceutical Investigation
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• v.7 no.1_4
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• pp.38-50
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• 1977

A study on the mechanism of biliary and urinary excretion of chloramphenicol has been performed in the dog. 1) Chloramphenicol administered intravenously to dogs with ligated renal pedicle, readily appeared in bile greater than in plasma. 6.9% of a 50mg /kg i. v. dose of chloramphenicol were excreted into bile within 100 minutes. During the same periods of above experiment, the bile/plasma concentration ratios(B/P ratios) were 46 to 87. 2) Chloramphenicol injected into the vein of dog was rapidly excreted into urine. 18% of the administered dose were excreted into urine within 70 minutes. In the same periods of this experiment, Ccm/Ccr ratios were greater than 1.0 in most cases. 3) In experiment of simultaneous measurement of biliary and urinary excretion of chloramphenicol, Ccm/Ccr ratios were less than 1.0 and B/P ratios were 50 to 52. 4) In experiment measured simultaneously biliary and urinary excretion both Ccm/Ccr and $C^Hcm$(hepatic clearance) were significantly declined by probenecid, but not affected by 2,4-DNP and aminophylline although 2,4-DNP increased only bile flow and aminophylline both bile and urine volume. 5) Ccm/Ccr and $C^Hcm$ were increased in proportion to increment of plasma concentration ranging from 3.3 to 30 mg% of chloramphenicol. But when plasma concentration were increased to 70mg %, Ccm/Ccr were not increased and $C_Hcm$ were reduced about 30% in comparison with values obtajned at 30mg% of chloramphenicol. 6) Free/Bound(free to bouid from) ratios ranging from 1.0 to 90.0mg% of chloramphenicol were 76.2+3.72% $(mean{\pm}S.E.)$ Above results suggest that chloramphenicol is excreted into bile by a process of active trasport, that excretion of chloramphenicol into urine was made up with dual process, reabsorption and secretion, and that renal secretion was attained by active trasport process although renal reabsorption process could not understand.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.14 no.1
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• pp.117-128
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• 2001

Gamiseunggal-tang(G-tang) has long been used for the treatment of acute and chronic allergic diseases(especially, urticaria) in oriental medicine. But, It's mechanism of action is not well investigated. Anal therapy is another way of taking medicine, which is not used in common situation but available for the treatment of colon and anal diseases. It is also used in GI tract diseases, Gynecological diseases, and pediatric diseases and so on. It is especially benefitable for patients who are in such situations as coma, severe vomiting, and swallowing difficulty. In this study, the author investigated the effects of G-tang by anal therapy on acute and chronic allergic reactions in murine model. The results obtained are as follows: 1. G-tang(0.01, 0.1 g/kg) inhibited the compound 48/80-induced ear swelling response in mice. Inhibitory effects of G-tang was significant (P<0.05) at the dose of 0.01 g/kg. 2. G-tang(0.001-0.1 g/kg) inhibited the cutaneous allergic reaction activated by anti-dinitrophenyl(DNP) IgE in rats. Especially, G-tang 0.01, 0.1 g/kg inhibited the cutaneous allergic reaction significantly. 3. G-tang(0.01-1 g/L) dose-dependently inhibited the compound 48/80-induced histamine release from the peritoneal mast cells. 4. G-tang(0.001-0.1 g/L) had a inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-${\alpha}$ production. Above results indicate that anal therapy of G-tang may be beneficial in the treatment of acute and chronic allergic diseases.

• The Journal of Korean Medicine
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• v.21 no.3
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• pp.57-67
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• 2000

Currently asthma is considered to be an inflammatory disease characterized by airway hyperresponsiveness and pulmonary eosinophilia, and mediated by Th lymphocytes expressing a Th 2 cytokine pattern. In many recent studies, molecular biological methods have been used to investigate the role of cytokines in pathogenesis and new therapeutic targets of asthma. Objectives: We aimed to identify the effect of Haepyoijin-tang on the transcriptional activities of cytokines involved in the asthma model. Materials and Methods: RBL-2H3 cell lines were used. Cells were stimulated with DNP-IgE or Calcium inophore+PMA for maximal gene expression. After 24 hours of Haepyoijin-tang-treatment, total cellular RNAs were collected using the Trizol solution method. Then the transcriptional activities of cytokines(IL-1, 4, 5, 10, 13, $TNF-{\alpha}$) were measured by RT-PCR with electrophoresis. Results: DNP-IgE and Calcium inophore+PMA induced IL-4/IL-5 production separately peaked at 3 hours after the stimulation, but the efficacy was better in the Calcium inophore+PMA group. In the IL-4 study, sample groups of 10%, 1 %, 0.01 % Haepyoijin-tang-treatment showed 83%, 98%, 96% of transcriptional activities compared to the control group. In the IL-5 study, sample groups of 10%, 1%, 0.1 %, 0.01 % Haepyoijin-tang showed 97%, 99%, 99%, 99% of transcriptional activities compared to the control group. In other studies any result was not obtained. Conclusions: This study shows that Haepyoijin-tang has an inhibitory effect on the transcription of IL-4 and IL-5 gene expression in RBL-2H3 cell lines. Advanced studies are required to investigate the mechanisms of inhibition by herbal medicine in the asthma model.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.15 no.2
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• pp.95-102
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• 2022

It is a study for the evaluation of the stability of the distribution automation system for the expansion of renewable energy. Through the Renewable Energy 3020 Implementation Plan, the government plans to expand new renewable energy and convert it to participatory energy that improves the quality of life of the people by 2030. The government has set a target of 20% of domestic supply energy for renewable energy generation by 2030. It is planning to establish more than 95 percent of its new facilities with clean energy such as solar power and wind power. By expanding the supply of renewable energy, new energy businesses and distributed power industry were fostered, and short-distance, low-voltage, and small-scale power generation were rapidly expanded rather than large-scale power development in the past. Due to this demand, the importance of power distribution facility operation has emerged and the need for distribution automation system is increasing. This paper discusses the development of a power distribution simulator for the performance and function evaluation of power distribution automation systems and presents the results of an interlocking test with the power distribution automation system. In order to introduce an advanced system into the power distribution system, it is necessary to take advantage of the transmission and distribution system. The DNP3.0 protocol is used in the distribution system and the IEC61850 protocol is used in the transmission and distribution system. It was concluded that the functions and performance of operations were satisfied when these two protocols are mixed and used in the distribution automation system.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.3
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• pp.348-355
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• 1989

This experiment was conducted to investigate the characteristics of alkaline protease from Aspergillus fumigatus which was isolated from soil as a superior strain for the production of the alkaline protease. The optimum temperature for enzyme activity was $50^{\circ}C$ and optimum pH was 9.0. The enzyme was stable at pH 8.0 to 10.0 and thermal inactivation was shown $30^{\circ}C$. The activity of the enzyme was increased by the addition of $Mn^{++},\;Cu^{++},\;Ba^{++},\;Mg^{++},\;$wheras it was inhibitied by $K^+,\;Fe^{+++},\;Ag^+,\;Pb^{++},\;Na^+,\;Ca^{++},\;Hg^+,\;Zn^{++}$. EDTA. 2, 4-DNP, ${\varepsilon}-amino$ caproic acid did not show inhibitory effect on the proteolytic activity of alkaline protease but P-chloromercuribenzoic acid inhibited the enzyme activity, indicating that reactive sulfhydryl group is required for the enzymatic activity. The reaction of this enzyme followed typical Michael-Menten Kinetics with the Km value of $8.33{\times}10^{-4}mole/{\ell}$ with the Vmax of $47.62{\mu}g/min$. This enzyme had stronger proteolytic activity than trypsin on substrate such as casin and hemoglibin.

• 제어로봇시스템학회:학술대회논문집
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• 1996.10b
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• pp.996-999
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• 1996

This paper introduces prototype design of 765kV Substation control system based on fiber optics LAN and distributed network protocol. Two major important facts, immunity of any surge which frequently occurs in Ultra High Voltage Substation and the most suitable communication protocol for substation control system, are considered on the system design conception. To meet the first fact, use of fiber optics cable and isolation transformer are considered, and to satisfy the second fact, DNP3.0(Distributed Network Protocol) which is IEC870-5 compatible is adopted.

• Analytical Science and Technology
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• v.9 no.2
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• pp.134-138
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• 1996

Cadmium was transported through the special carrier system into the cells of cotyledons, hypocotyls, and roots spring radish young plants. The transport of cadmium was inhibited by metabolic inhibitor, like DNP. The $K_m$ values for cadmium were 0.7 ppm for cotyledons, 1.72 ppm for hypocotyls, and 0.3 ppm for roots, and $V_{max}$ for cadmium was $40ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for cotyledons, $313ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for hypocotyls, and $606ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for roots. Cadmium cannot prove to be inducer for proline accumulation. Therefore, proline accumulation cannot be used as a marker to test the level of heavy metal pollution.