• ALGAE
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• 제38권2호
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• pp.127-139
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• 2023

Cryptic stages of diverse macroalgae present in natural substrata, "the bank of microscopic forms", were isolated into clonal cultures and identified based on both morphological characteristics and DNA barcoding. Approximately 120 clonal isolates from 308 natural substratum samples were collected from the entire coastline of Kuwait. Amongst these isolates, 77 (64%) were identified through DNA barcoding using the nuclear ribosomal small subunit, RuBisCO spacer (ITS2, tufa, rbcL, psaA, and psbA) and sequencing. Twenty-six isolates (34%) were identified in the division Chlorophyta, 18 (23%) as Phaeophyceae, and 33 (43%) as Rhodophyta. For all DNA sequences in this study, species-level cut off applied was ≥98% homology which depend entirely on the markers used. Three putative new records of Chlorophyta new for the Arabian Gulf were made: Cladophora laetevirens (Dillwyn) Kützing, Ulva torta (Mertens) Trevisan and Ulvella leptochaete (Huber) R. Nielsen, C. J. O'Kelly & B. Wysor in Nielsen, while Cladophora gracilis Kützing and Ulva ohnoi M. Hiraoka & S. Shimada are new records for Kuwait. For Phaeophyceae, Ectocarpus subulatus Kützing and Elachista stellaris Areschoug were new records for the Gulf and Kuwait. In the Rhodophyta, Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer, Ceramium affine Setchell & N. L. Gardner, Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel and Dasya caraibica Børgesen are new records for the Gulf and Kuwait, while the red alga Stylonema alsidii (Zanardini) K. Drew is a new record for Kuwait. Several isolates identified corresponded to genera not previously reported in Kuwait and / or the Arabian Gulf, such as Porphyrostromium Trevisan, a new genus from the Bangiales, and two unidentified species for the Planophilaceae Škaloud & Leliaert. The isolates cultivated from substrata enhance understanding of the marine macroalgal diversity in the region and confirmed that the Germling Emergence Method is suitable for determining the actual diversity of a given study area through isolation from cryptic life-history phases.

• Journal of Plant Biotechnology
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• 제45권1호
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• pp.9-16
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• 2018

DNA 바코딩 기술은 다양한 약용식물 종들의 기원을 확인하기 위해 폭넓게 이용되고 있는 연구방법이다. 그러나, 시중에 판매되고 있는 유사 식물종을 재료로 사용한 상품이나 혼재되어 있는 상품에서 확인하고자 하는 약용식물을 선별 가능한 실질적인 기술의 개발은 아직 많이 미흡한 실정이다. 최근에는 보다 신속하고 정확도가 높은 기술을 개발하고자 DNA barcoding (Bar) 기술과 high-resolution melting (HRM) curve pattern 분석기술을 혼합한 Bar-HRM 분석기술을 이용한 연구가 진행 중에 있다. 본 리뷰논문에서는 국제적인 시장에서 다양한 기원의 약용식물 판별에 실질적으로 적용 가능한 Bar-HRM 기술의 최근의 발전 과정과 그 이용에 대해서 정리하였다. 다양한 연구들을 통해서 일부 성공적인 결과들이 보고되고 있지만, 제한된 DNA 바코드 및 단일염기다형성(Single Nucleotide Polymorphism, SNP) 등 아직 해결되어야 할 과제들이 많다. 특히, 핵 내 바코드로는 ribosomal DNA의 internal transcribed sequence (ITS)단편 이외에는 보고된 사례가 한건도 없었다. 또한, 약용식물을 끓는 물로 추출하여 가공한 약탕, 잼, 젤리, 쥬스 등의 제품은 DNA 단편이 분해되어 분리가 안 되는 경우에는 DNA바코딩 기술을 적용하기가 곤란한 것으로 알려져 있으나 비교적 짧은 DNA단편이 요구되는 Bar-HRM 분석기술을 이용하여 일부 성공한 보고도 있어 향후 그 응용사례가 증가할 것으로 전망된다.

• Molecules and Cells
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• 제46권2호
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• pp.74-85
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• 2023

Single-cell research has provided a breakthrough in biology to understand heterogeneous cell groups, such as tissues and organs, in development and disease. Molecular barcoding and subsequent sequencing technology insert a single-cell barcode into isolated single cells, allowing separation cell by cell. Given that multimodal information from a cell defines precise cellular states, recent technical advances in methods focus on simultaneously extracting multimodal data recorded in different biological materials (DNA, RNA, protein, etc.). This review summarizes recently developed single-cell multiomics approaches regarding genome, epigenome, and protein profiles with the transcriptome. In particular, we focus on how to anchor or tag molecules from a cell, improve throughputs with sample multiplexing, and record lineages, and we further discuss the future developments of the technology.

• 한국어류학회지
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• 제34권2호
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• pp.119-126
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• 2022

본 연구는 동중국해에서 수집한 치어(체장 5.2 mm)의 종을 COI 바코딩을 통해 남방표문쥐치(Naso hexacanthus)로 동정하고, 형태를 처음으로 보고한다. 치어는 마름모꼴의 몸통으로 등지느러미와 뒷지느러미의 첫 극조는 길고 톱니가 나 있었다. 뒷지느러미 기저부에 네 개의 흑색소포, 미병부에 밀집한 흑색소포, 뇌 표면에 퍼져 있는 흑색소포가 있었다. 작은 가시 한 개가 각각 콧등과 눈 뒤에 있었고, 톱니 모양의 돌기가 머리, 눈 위, 전새개골 내부와 외부, 새개골 아래와 옆 부분에 발달하였다. COI 바코딩으로 동정한 남방표문쥐치 치어의 형태적 특징은 치어의 종 동정에 유용할 것이다.

• Animal Systematics, Evolution and Diversity
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• 제36권1호
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• pp.10-14
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• 2020

Pagurus is the most diverse hermit crab genus in Korea. In this study, the cytochrome c oxidase subunit I (COI) and 16S rRNA of 24 individuals from four Korean Pagurus species (i.e., 7 Pagurus brachiomastus, 8 P. proximus, 8 P. simulans, and 1 P. rectidactylus) were sequenced and analyzed. No genetic difference was found between the COI and 16S rRNA sequences of P. brachiomastus and P. simulans, and the COI sequences of P. rectidactylus and P. quinquelineatus (comparative species from NCBI). Considering the morphological and ecological characteristics together, we assume that P. simulans and P. rectidactylus are subspecies of P. brachiomastus and P. quinquelineatus, respectively. This study should facilitate further research on the taxonomic status of these species.

• 한국수산과학회지
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• 제53권5호
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• pp.794-799
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• 2020

In April 2019, a single Peristediidae juvenile (14.1 mm SL) was collected from the waters off of Jeju-do Island, Korea. DNA barcoding identified the juvenile as Peristedion liorhynchus, an unrecorded species in Korea. P. liorhynchus has eight dorsal fin spines, 22 dorsal and 19 anal fin rays, and a long third pectoral fin ray that passes through the middle of the tail at the juvenile stage. Juvenile also have large heads, extensive head spination, and serrated edges on the ocular and parietal spines. This is the first record of P. liorhynchus in Korea; therefore, we propose the new Korean name, "Nam-bang-hwang-seong-dae".

• Animal Systematics, Evolution and Diversity
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• 제35권3호
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• pp.136-139
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• 2019

The pulmonate gastropod Ellobium chinense (Pfeiffer, 1864) is an endangered marine species along the South Korean coasts due to habitat destruction and population declines. We sequenced the cytochrome c oxidase subunit 1 (COI) of 25 E. chinense specimens collected from five coastal sites in South Korea, and identified 16 unique haplotypes. The maximum intraspecific variation among individuals was 1.6%, while interspecific differences from another ellobiid species, Auriculastra duplicata (Pfeiffer, 1854), ranged from 21.9 to 23.0%. Our barcoding data will be useful to elucidate the phylogenetic relationships among pulmonate gastropods and infer the population genetic structure of E. chinense.

• 한국수산과학회지
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• 제54권3호
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• pp.340-349
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• 2021

Three specimens (33.6-44.8 mm in standard length) of the family Bregmacerotidae, collected from the waters off south-western Jeju-do Island in October 2016, were identified as Bregmaceros nectabanus on the basis of morphological and molecular methods (DNA barcoding). This species is characterized by having 50-52 2nd dorsal fin rays, 52-53 anal fin rays, 13 principal caudal fin rays, an unpigmented abdomen, irregularly distributed dorsal chromatophores, and the origin of a dorsal fin slightly behind the anal fin. This is the first reliable record of B. nectabanus in Korea. According to Yoo et al. (1992), we propose a Korean name "Tae-Pyeong-Yang-Nal-Gae-Myeol" for this species. Also, we suggest key to species of these eight bregmacerotids from the northwestern Pacific.

• Animal Systematics, Evolution and Diversity
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• 제37권3호
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• pp.225-228
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• 2021

Eleven Gonioctena species have been recorded in the Korean Peninsula. Most species in the genus have very similar coloration and morphology. The male genitalia is generally used as the only reliable diagnostic character differentiating closely related species, but it is still difficult to identify females to species accurately. Here, we determined the COI barcodes of two Gonioctena species described from Korea and give morphological descriptions. The interspecific genetic divergence (Kimura 2-parameter) among the Gonioctena ranged from 0.073 to 0.138 and averaged 0.108. The pairwise genetic distance between the two Korean species(0.102) is similar to the average interspecific divergence. We predict that these analyses will help to resolve taxonomic issues and find cryptic species.

• 생태와환경
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• 제52권4호
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• pp.385-393
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• 2019

Chironomid communities are indicators of water pollution because of their ability to thrive under freshwater conditions. However, it is difficult to distinguish between chironomid larvae based on morphology. DNA barcoding, based on nucleotide sequences of marker genes, can be used to identify chironomid larvae. Samples of chironomid larvae were collected from Gwangju Stream and Pungyeongjeong Stream, tributaries of the Yeongsan River in South Korea. We identified 3 subfamilies, 13 genera, 16 species, and 1 cryptic species. There were 7 genera and 10 species from the subfamily Chironominae, 5 genera and 5 species from subfamily Orthocladiinae, 1 genus and 1 species from subfamily Tanipodinae, and the cryptic chironomid species of the family Chironomidae. There were 21 individuals from, 7 species and 1 cryptic species from the Gwangju Stream and 24 individuals, belonging to 10 species from the Pungyeongjeong Stream. The only species detected in both streams was Cricotopus bicinctus. The relationship between water quality and the species detected was difficult to explain, but the number of species showed a tendency to increase at sites where water quality was poor. Additional investigations and studies are needed to understand the relationship between water quality and the chironomid species occurring in these two streams.