• Title/Summary/Keyword: DM-domain

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Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin

  • Phadungsil, Wansika;Grams, Rudi
    • Parasites, Hosts and Diseases
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    • v.59 no.2
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    • pp.173-178
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    • 2021
  • The DM9 domain is a protein unit of 60-75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with the uptake of microbial organisms. Likewise, in the trematode Fasciola gigantica DM9-1 showed intracellular relocalization following microbial, heat and drug stress. In the present research, we show that FgDM9-1 is a lectin with a novel mannose-binding site that has been recently described for the protein CGL1 of Crassostrea gigas. This property allowed FgDM9-1 to agglutinate gram-positive and -negative bacteria with appropriate cell surface glycosylation patterns. Furthermore, FgDM9-1 caused hemagglutination across all ABO blood group phenotypes. It is speculated that the parenchymal located FgDM9-1 has a role in cellular processes that involve the transport of mannose-carrying molecules in the parenchymal cells of the parasite.

Molecular Cloning and Expression of DMRT Gene in Protogynous Wrasse, Halichoeres tenuispinis

  • Jeong, Hyung-Bok;Park, Ji-Gweon;Park, Jin-Young;Jin, Young-Jun;Yang, Myung-Cheon;Hyun, Kyung-Man;Kim, Gi-Ok;Kim, Se-Jae
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.64-64
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    • 2003
  • The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

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Switching current density for spin transfer torque magnetic random access memory with Dzaloshinskii-Moriya Interaction

  • Song, Kyungmi;Lee, Kyung-Jin
    • Proceedings of the Korean Magnestics Society Conference
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    • 2015.05a
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    • pp.78-79
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    • 2015
  • We investigate the switching current for various cell diameters and DM interaction. We find that the current density for switching can depend strongly on the cell size when the switching is governed by the domain wall motion. Moreover the switching current density is also strongly influenced by DM interaction. In the presentation, we will discuss the effect of domain wall formation and more various DMI constant on the switching current desity in detail.

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DIFFUSION PIECEWISE HOMOGENIZATION VIA FLUX DISCONTINUITY RATIOS

  • Sanchez, Richard;Dante, Giorgio;Zmijarevic, Igor
    • Nuclear Engineering and Technology
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    • v.45 no.6
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    • pp.707-720
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    • 2013
  • We analyze piecewise homogenization with flux-weighted cross sections and preservation of averaged currents at the boundary of the homogenized domain. Introduction of a set of flux discontinuity ratios (FDR) that preserve reference interface currents leads to preservation of averaged region reaction rates and fluxes. We consider the class of numerical discretizations with one degree of freedom per volume and per surface and prove that when the homogenization and computing meshes are equal there is a unique solution for the FDRs which exactly preserve interface currents. For diffusion submeshing we introduce a Jacobian-Free Newton-Krylov method and for all cases considered obtain an 'exact' numerical solution (eight digits for the interface currents). The homogenization is completed by extending the familiar full assembly homogenization via flux discontinuity factors to the sides of regions laying on the boundary of the piecewise homogenized domain. Finally, for the familiar nodal discretization we numerically find that the FDRs obtained with no submesh (nearly at no cost) can be effectively used for whole-core diffusion calculations with submesh. This is not the case, however, for cell-centered finite differences.

Non-data Aided Timing Phase Recovery Scheme for Digital Equalization of Chromatic Dispersion and Polarization Mode Dispersion

  • Park, Jang-Woo;Chung, Won-Zoo;Park, Jong-Sun;Kim, Sung-Chul
    • Journal of the Optical Society of Korea
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    • v.13 no.3
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    • pp.367-372
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    • 2009
  • In this paper we propose an electronic domain timing phase selection scheme for the optical communication systems suffering from inter-symbol-interference (ISI) distortion due to chromatic dispersion (CD) or polarization mode dispersion (PMD). In the presence of CD/PMD a proper timing phase selection is important for discrete time domain equalizers, since different timing phases produce different nonlinear ISI channels of different severity. The proposed timing phase recovery scheme based on dispersion minimization (DM) practically approximates the optimal minimum mean squared error (MMSE) timing phase without training signals which reduces overall throughput substantially, especially in time-varying channels such as PMD. The simulation results show that the proposed DM timing agrees with MMSE timing phase, under proper normalization of the received signals, for various dispersion and OSNR.

Engineering of Recombinant Escherichia coli Towards Methanol Sensing Using Methylobacterium extroquens Two-component Systems

  • Selvamani, Vidhya;Ganesh, Irisappan;Chae, Sowon;Maruthamuthu, Murali kannan;Hong, Soon Ho
    • Microbiology and Biotechnology Letters
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    • v.48 no.1
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    • pp.24-31
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    • 2020
  • Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK's) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.

Neural Network-based Decision Class Analysis with Incomplete Information

  • Kim, Jae-Kyeong;Lee, Jae-Kwang;Park, Kyung-Sam
    • Proceedings of the Korea Database Society Conference
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    • 1999.06a
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    • pp.281-287
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    • 1999
  • Decision class analysis (DCA) is viewed as a classification problem where a set of input data (situation-specific knowledge) and output data (a topological leveled influence diagram (ID)) is given. Situation-specific knowledge is usually given from a decision maker (DM) with the help of domain expert(s). But it is not easy for the DM to know the situation-specific knowledge of decision problem exactly. This paper presents a methodology fur sensitivity analysis of DCA under incomplete information. The purpose of sensitivity analysis in DCA is to identify the effects of incomplete situation-specific frames whose uncertainty affects the importance of each variable in the resulting model. For such a purpose, our suggested methodology consists of two procedures: generative procedure and adaptive procedure. An interactive procedure is also suggested based the sensitivity analysis to build a well-formed ID. These procedures are formally explained and illustrated with a raw material purchasing problem.

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Neural Network-based Decision Class Analysis with Incomplete Information

  • 김재경;이재광;박경삼
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 1999.03a
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    • pp.281-287
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    • 1999
  • Decision class analysis (DCA) is viewed as a classification problem where a set of input data (situation-specific knowledge) and output data(a topological leveled influence diagram (ID)) is given. Situation-specific knowledge is usually given from a decision maker (DM) with the help of domain expert(s). But it is not easy for the DM to know the situation-specific knowledge of decision problem exactly. This paper presents a methodology for sensitivity analysis of DCA under incomplete information. The purpose of sensitivity analysis in DCA is to identify the effects of incomplete situation-specific frames whose uncertainty affects the importance of each variable in the resulting model. For such a purpose, our suggested methodology consists of two procedures: generative procedure and adaptive procedure. An interactive procedure is also suggested based the sensitivity analysis to build a well-formed ID. These procedures are formally explained and illustrated with a raw material purchasing problem.

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Heart rate monitoring and predictability of diabetes using ballistocardiogram(pilot study) (심탄도를 이용한 연속적인 심박수 모니터링 및 당뇨 예측 가능성 연구(파일럿연구))

  • Choi, Sang-Ki;Lee, Geo-Lyong
    • Journal of Digital Convergence
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    • v.18 no.8
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    • pp.231-242
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    • 2020
  • The thesis presents a system that continuously collects the human body's physiological vital information at rest with sensors and ICT information technology and predicts diabetes using the collected information. it shows the artificial neural network machine learning method and essential basic variable values. The study method analyzed the correlation between heart rate measurements of BCG and ECG sensors in 20 DM- and 15 DM+ subjects. Artificial Neural Network (ANN) machine learning program was used to predictability of diabetes. The input variables are time domain information of HRV, heart rate, heart rate variability, respiration rate, stroke volume, minimum blood pressure, highest blood pressure, age, and sex. ANN machine learning prediction accuracy is 99.53%. Thesis needs continuous research such as diabetic prediction model by BMI information, predicting cardiac dysfunction, and sleep disorder analysis model using ANN machine learning.