• Proceedings of KOSOMES biannual meeting
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• 2003.05a
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• pp.191-200
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• 2003

Gunsan coastal area is one of region increasing pollution problems. One of the most important factors that cause eutrophication is nutrient materials containing nitrogen and phosphorus which stem from excreation of terrestial sources. At this study, the three-dimensional numerical hydrodynamic and ecosystem model, which was developed by Institute for Resources and Environment of Japan, were applied to analyze the processes affecting the eutrophication. The residual currents, which were obtained by integrating the simulated tidal currents over 1 tidal cycle, showed the presence of a typical. Density driven currents were generated westward at surface and eastward at the bottom in Geum estuary area where the fresh waters are flowing into. The ecosystem model was calibrated with the data surveyed in the field of the study area in annual average. The simulated results of DIN were fairly good coincided with the observed values within relative error of 32.39%. correlation coefficient(r) of 0.99. In the case of DIP, the simulated results were fairly good coincided with the observed values within relative error of 24.26%, correlation coefficient (r) of 0.82. The simulations of DIN and DIP concentrations were performed using ecosystem model under the conditions of 20 ∼ 80% pollution load reductions from pollution sources. In study area, concentration of DIN and DIP were reduced to 20∼80% and under 10% in case of the 80% reduction of the input loads from fresh water respectively. But pollution loads from sediment had hardly affected DIN and DIP concentration. For the environment management of coastal areas, in case of Kunsan area, the most important pollution sources affecting eutrophication phenomenon were found to be the input loads from fresh water.

• Korean Journal of Microbiology
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• v.29 no.6
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• pp.392-396
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• 1991

Mu d1(Ap lac) bacteriophage can be used to search for genes which are members of a common regulatory network without having to know the functions of the genes in advance. Aim was for obtaining the loci in the SOS network as well as temperature inducible loci. For this purpose, recA441 allele was used. This allele encodes a thermosensitive recA gene product; thus, the recA441 allele can be activated upon temperature upshift without by external DNA damage. Approximately 10, 000 colonies were screened, and then searched for the colonies which expressed .betha.-galactosidase higher level at 42.deg.C than at 30.deg.C. The strains identified fell into two dlasses; (i) ones in which the increased expression was $recA^{+}$ $lexA^{+}$ -dependent, that is, din(damage-inducible) genes which were due to the activation of recA441 allele and (ii) ones in which the increased expression was $recA^{+}$ $lexA^{+}$ -independent and only temperature-inducible, tin genes. Rough mapping position was obtained for these genes.

• Ocean and Polar Research
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• v.29 no.4
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• pp.349-366
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• 2007

Seasonal variations of various physicochemical components (temperature, salinity, pH, DO, COD, DOC, nutrients-silicate, DIN, DIP) and potential limiting factor for phytoplankton primary production were studied in the surface water of semi-enclosed Masan Bay. Seasonal variations of nutrient concentrations, with lower values in summer and winter, and higher in fall, are probably controlled by freshwater loadings to the bay, benthic flux and magnitude of occurrence of phytoplankton communities. Their spatial distributional patterns are primarily dependent on physical mixing process between freshwater and coastal seawater, which result in a decreasing spatial gradient from inner to outer part of the bay. In the fall season of strong wave action, the major part of nutrient inputs (silicate, ammonium, dissolved inorganic phosphorus) comes from regeneration (benthic flux) at sediment-water interface. During the summer period, high Si:DIN and Si:DIP and low DIN:DIP relative to Redfield ratios suggest a N- and secondarily P-deficiency. During other seasons, however, silicate is the potential limiting factor for primary production, although the Si-deficiency is less pronounced in the outer region of the bay. Indeed, phytoplankton communities in Masan Bay are largely affected by the seasonal variability of limiting nutrients. On the other hand, the severe depletion of DIN (relatively higher silicate level) during summer with high freshwater discharge probably can be explained by N-uptake of temporary nanoflagellate blooms, which responds rapidly to pulsed nutrient loading events. In Masan Bay, this rapid nutrient consumption is considerably important as it can modify the phytoplankton community structures.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.4
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• pp.456-463
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• 1996

The purpose of the present study is to estimate the regional and seasonal variations of dissolved inorganic nitrogen (DIN) flux across the sediment-water interface of the inner and central areas of Hiroshima Bay from August 1994 to May 1995. In addition it compares the measured methods and estimates the effect of DIN released from sediment to the primary production of Hiroshima Bay. One method used in this study is to calculate DIN flux from a concentration gradient between sediment porewaters and the overlying water, and the other method is to measure DIN flux from the sediment-core experiment. The fluxes of $NH_{4}^{+}-N\;and\;NO_{2}^{+}\;+\;NO_{3}^{-}-N$ in the inner area were higher than those in central area, all of which showed seasonal variation. $NH_{4}^{+}-N$ flux was maximum in August, while $NO_{2}^{-}\;+\;NO_{3}^{-}-N$ flux was high in January compared with the other seasons. The calculated $NH_{4}^{+}-N\;and\;NO_{2}^{-}+NO_{3}^{-}-N$ fluxes from sediments were $18.2\~60.8\;{\mu}g-at/m^2{\cdot}hr\;and\;0.24\~18.2\;{\mu}g-at/m^2{\cdot}hr$, respectively. The measured $NH_{4}^{+}-N\;and\;NO_{2}^{-}+NO_{3}^{-}-N$ fluxes across the sediment-water interface were $2.00\~111\;{\mu}g-at/m^2{\cdot}hr\;and\;-265\~82.9\;{\mu}g-at/m^2{\cdot}hr$, respectively. The former was lower than the tatter. The calculated $NH_{4}^{+}-N$ flux showed closer relation to environmental factors (dissolved of gen in the overlying water, temperature and redox condition of the sediments) than the measured one did. On the other hand, in the case of $NO_{2}^{-}+NO_{3}^{-}-N$ flux both the calculated and the measured showed little relation to environmental factors, while they turned out to have stronger relation with their concentration in sediments. DIN released from the sediment is expected to support about $25\%\~67\%$ of the primary production in Hiroshima Bay.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.1
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• pp.35-46
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• 2009

In order to investigate spatial and temporal distributional characteristics of major water qualities in the Saemangeum area during the Saemangeum dike construction, salinity, COD, dissolved nutrients(DIN, Silicate) and heavy metals were analyzed from the surface water collected in April, May, August and November 2002. The overall value of Salinity, COD, DIN, and silicate in surface waters were in the range of $13.08{\sim}31.96\;psu$, $0.12{\sim}3.43\;mg/L$, $0.001{\sim}2.638\;mg/L$, and $0.010{\sim}3.181\;mg/L$, respectively. The COD and DIN in each survey showed the highest concentration at the mouth of Mangyeong river estuary(St. 1) where freshwater flow into the Saemangeum area. The concentrations of nutrients were high in the inner part of the Saemangeum dike with low-salinity, and low nutrients in the outer part of the dike with high-salinity, which strongly indicated that concentrations were adjusted by physical mixing. The ranges of dissolved metals and acid-soluble Hg in surface seawater were $0.006{\sim}0.115{\mu}g/L$ for Co, $0.26{\sim}0.114{\mu}g/L$ for Ni, $0.14{\sim}0.93{\mu}g/L$ for Cu, $0.04{\sim}0.53{\mu}g/L$ for Zn, $0.010{\sim}0.043{\mu}g/L$ for Cd, $0.010{\sim}0.795{\mu}g/L$ for Pb, and $0.25{\sim}4.16{\mu}g/L$ for Hg. The highest concentrations of some metals except for Cd were found at the estuary(Sts. 1 or 3). In most cases, a decreasing order of metal concentrations towards open sea(low-salinity$\rightarrow$high-salinity) was observed and showed positive relationship with DIN and silicate caused by land base pollutants input. On the other hand, due to Cd desorption from suspended solids in saline water, dissolved Cd concentrations were high in high-salinity area and low in low-salinity. In November, Co, Zn, Cu and Pb were relatively high in the northern area of the outer-side of Saemangeum, which was only influenced by the Geum river discharge. The concentrations of most dissolved metals of this study were lower than those of the past data in this area, but higher than those in Lena river estuary under the pristine environment.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.455-461
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• 2002

limiting nutrients for phytoplankton were determined by dissored inorganic nitrogen/phosphorous (DIN/DIP) in situ and algal bioassay experiment in the Seomjin River estuary during a study period from March 1999 to October 2001. DIN/DIP ranged from 14.7 to 681.1 during the study period. DIN/DIP was over 16 at the upper and middle estuarine region where salinity was lower than 25 psu and chlorophyll a concentration was high, probably indicating P-limitation in this region while the ratio was less than 16 at the high saline (> 25 psu) region, reflecting the supply of DIP from Gwangyang Bay and thereby indicating N-limitation at the lower estuarine region. These results suggested that the spatial distribution of DIN/DIP in the study region was controlled by the high supply of phosphate from Gwangyang Bay, the low input from Seomjin River and the active uptake by phytoplankton within the estuarine system. The bioassay experiments using Skeletonema costatum, Thalaasiosira rotula and in situ phytoplankton assemblage displayed relatively higher growth of phytoplankton in the P-added culture media, indicating P-limitation. This result was well consistent with the spatial distribution of inorganic nutrients. S. costatum showed a rapid adaptation to the low salinity compared to other phytoplankton species. This phenomenon seemed to account for the strong (> $90\%$ in total cell number) S. costatum bloom in autumn in this estuary. Moreover, although phytoplankton growth rate was higher in the P-added culture media at the end of culture experiment of in situ phytoplankton, the fast growth in the trace metal-added media at the beginning of the experiment suggested a possibility of limitation by other micro-nutrients such as trace metal and vitamin etc.

• Steel and Composite Structures
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• v.18 no.1
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• pp.149-163
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• 2015

Orthotropic steel highway bridges exist almost everywhere in world, especially in Europe. The design of these bridges started very early in 20th century and ended with a conventional orthotropic steel bridge structure, which is today specified in DIN FB 103. These bridges were mostly built in 1960's and exhibit damages in steel structural parts. The primary reason of these damages is the high pressure that is induced by wheel- loads and therefore damages develop especially in heavy traffic lanes. Constructive rules are supplied by standards to avoid damages in orthotropic steel structural parts. These rules are first given in detail in the standard DIN 18809 (Steel highway- and pedestrian bridges- design, construction, fabrication) and then in DIN- FB 103 (Steel bridges). Bridges built in the past are today subject to heavier wheel loads and the frequency of loading is also increased. Because the vehicles produced today in 21st century are heavier than before and more people have vehicle in comparison with 20th century. Therefore dimensioning or strengthening of orthotropic steel bridges by using stiffer dimensions and shorter spans is an essence. In the scope of this study the complex geometry of conventional steel orthotropic bridge is generated by FE-Program and the effects of cross beam web thickness and cross beam span on steel bridge are assessed by means of a parameter study. Consequently, dimensional and constructional recommendations in association with cross beam thickness and span will be given by this study.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.225-226
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• 2003

육상으로부터 유입되는 오염부하와 그로 인한 제주항의 해양수질 환경특성을 조사한 결과는 다음과 같다. 제주항의 수질특성을 각 항목별로 보면, 용존산소(DO)의 농도분포는 평균 7.3 mg/L의 범위를 나타내었고, 그리고 COD의 농도분포는 평균 2.2 mg/L로 대상해역의 대부분이 III등급 해역에 속하였으며 해역의 부영양화에 관계되는 영양염인 DIN과 DIP는 각각 평균 12.20 ${\mu}g$-at/L와 평균 1.18 ${\mu}g$-at/L로 2001년 개정 전 해역수질환경기준인 농도와 비교해 보면 평균농도에서 III등급을 초과하는 것을 알 수 있었다. 산지천의 수질특성을 보면 용존산소(DO)는 평균값은 7.4 mg/L로 하천수질기준 II등급에 해당되었으며 BOD의 경우는 평균값으로는 3.4 mg/L를 보여 하천수질기준 III등급에 해당되었다. 그리고 DIN과 DIP의 경우는 각각 4.696~5.410 mg/L와 0.306~0.332 mg/L의 범위를 보여 각각의 평균값은 5.110 mg/L과 0.315 mg/L였다. 산지천의 하천 유입부하량을 산정한 값을 보면 COD의 경우는 0.30 ton/day의 값을 보였고 DIN과 DIP의 부하량은 각각 300.0 kg/day와 18.0 kg/day의 값을 보였다. 이상에서 조사된 제주항의 수질특성과 산지천 유입부하량은 제주항의 수질관리를 위한 물질순환모델을 적용할 때 기초입력자료로 제공될 수 있을 것이다.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2002.06a
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• pp.5-23
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• 2002

Motivation: Protein-protein interaction plays a critical role in the biological processes. The identification of interacting proteins by bioinformatical methods can provide new lead In the functional studies of uncharacterized proteins without performing extensive experiments. Results: Protein-protein interactions are predicted by a computational algorithm based on the weighted scoring system for domain interactions between interacting protein pairs. Here we propose potential interaction domain (PID) pairs can be extracted from a data set of experimentally identified interacting protein pairs. where one protein contains a domain and its interacting protein contains the other. Every combinations of PID are summarized in a matrix table termed the PID matrix, and this matrix has proposed to be used for prediction of interactions. The database of interacting proteins (DIP) has used as a source of interacting protein pairs and InterPro, an integrated database of protein families, domains and functional sites, has used for defining domains in interacting pairs. A statistical scoring system. named "PID matrix score" has designed and applied as a measure of interaction probability between domains. Cross-validation has been performed with subsets of DIP data to evaluate the prediction accuracy of PID matrix. The prediction system gives about 50% of sensitivity and 98% of specificity, Based on the PID matrix, we develop a system providing several interaction information-finding services in the Internet. The system, named PreDIN (Prediction-oriented Database of Interaction Network) provides interacting domain finding services and interacting protein finding services. It is demonstrated that mapping of the genome-wide interaction network can be achieved by using the PreDIN system. This system can be also used as a new tool for functional prediction of unknown proteins.

• Proceedings of the KSRS Conference
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• 2001.03a
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• pp.128-132
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• 2001

본 연구의 수행을 위해 23개의 JERS-1 SAR 와 두 개의 ERS-2 SAR 자료를 이용하였다. 비록 ERA-2 pair로부터 생성된 인터훼로그램(interferogram)은 70일의 짧은 간격 자료임에도 불구하고 수목, 구름, 눈 등에 의한 temporal decorrelation 과 낮은 관측각에 의한 layover등에 의해 인터훼로그램의 질이 좋지 않아 분석에 이용될 수 없었다. 반면에 JERS-1 SAR(L 밴드) 자료간의 pair는 매우 긴 시간 간격에도 불구하고 비교적 높은 긴밀도를 가지고 있어, 본 연구지역과 같이 지표 변화률이 매우 느릴 것으로 예상되는 지역에서 장기간의 변화를 관측하기에는 적합하다. 우리는 altitude of ambiguities가 매우 큰 3개의 인터훼로그램과 2-pass, 3-pass DInSAR 방법을 사용하여 1992년 9월과 1998년 10월 까지 약 6년 동안의 지표변위를 관측하였다. 다양한 시간 간격(704, 1056, 1100, 1118, 1232, 2112 days) 별로의 차분 인터훼로그램(differential interferogram)의 결과와 그들간의 상호관계성를 이용하여 연구지역의 지표변위를 분석하였다. 2-pass, 3-pass, 그리고 altitude of ambiguity가 큰 인터훼로그램으로부터 관측된 결과 모두, 백두산 산체와 남서쪽에 위치하고 있는 홍두산을 중심으로 수십km에 걸쳐서 지표가 상승하고 있음을 지시한다. 계산된 지표상승률은 1년에 약 9cm 정도이다.