• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9283-9289
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• 2014

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Materials and Methods: This study investigated alterations in the displacement loop (d-loop) region of mitochondrial DNA (mtDNA) as a risk factor and diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Using mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, the first 450 bp of the d-loop region were amplified and successfully sequenced. Results: This revealed 132 mutations at 25 positions in this region, with a mean of 6 alterations per subject. The d-loop alterations in mtDNA in subjects were all identified as single nucleotide polymorphisms in a homoplasmic distribution pattern. Mutant alleles were observed in all subjects with individual frequency rates of up to 95%. Thirteen mutant alleles in the d-loop region of mtDNA occurred with a high frequency. Novel alleles and locations were also identified in the d-loop of mtDNA as follows: 89 G insertions (40%), 95 G insertions (13%), 182 C/T substitutions (5%), 308 C insertions (19%), and 311 C insertions (80%). The findings of this study need to be replicated to be confirmed. Conclusions: Further investigation of the relationship between mutations in mitochondrial d-loop genes and incidence of acute lymphoblastic leukemia is recommended.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.757-760
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• 2015

Aim: To present the epidemiologic data (age, gender, size etc) and histopathologic and immunohistochemical features of cases of rhabdomyosarcoma (RMS) reported in our department. Settings: Section of Histopathology, Department of Pathology and Microbiology, Aga Khan University Hospital, Karachi, Pakistan. Duration: All cases of RMS diagnosed between January 1, 2003 and December 31, 2012 were included in the study. Results: A total of 277 cases were included. Embryonal rhabdomyosarcoma (ERMS) was by far the dominant histologic type (87.4%) followed by alveolar type (ARMS)(9.4%). ERMS was much more common in males (64.0%)and over 65% of cases occurred in the first decade of life (over 90% in the first two decades). Head and neck region was commonest site for ERMS (46.7%), followed by the genitourinary system (16.1%). Over 65% cases of ARMS occurred in the extremities. Over 80% cases of ARMS occurred in the first 3 decades of life. Immunohistochemical staining for Desmin and MyoD1 was positive in 96.7% and 85.4% respectively. Conclusions: The epidemiologic data and microscopic findings in our patients are similar to international published data on rhabdomyosarcoma.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.3675-3686
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• 2016

Moringa oleifera Lam, family Moringaceae, is a perennial plant which is called various names, but is locally known in Malaysia as ''murungai'' or ''kelor''. Glucomoringin, a glucosinolate with from M. oleifera is a major secondary metabolite compound. The seeds and leaves of the plant are reported to have the highest amount of glucosinolates. M. oleifera is well known for its many uses health and benefits. It is claimed to have nutritional, medicinal and chemopreventive potentials. Chemopreventive effects of M. oleifera are expected due to the existence of glucosinolate which it is reported to have the ability to induce apoptosis in anticancer studies. Furthermore, chemopreventive value of M. oleifera has been demonstrated in studies utilizing its leaf extract to inhibit the growth of human cancer cell lines. This review highlights the advantages of M. oleifera targeting chemoprevention where glucosinolates could help to slow the process of carcinogenesis through several molecular targets. It is also includes inhibition of carcinogen activation and induction of carcinogen detoxification, anti-inflammatory, anti-tumor cell proliferation, induction of apoptosis and inhibition of tumor angiogenesis. Finally, for synergistic effects of M. oleifera with other drugs and safety, essential for chemoprevention, it is important that it safe to be consumed by human body and works well. Although there is promising evidence about M. oleifera in chemoprevention, extensive research need to be done due to the expected rise of cancer in coming years and to gain more information about the mechanisms involved in M. oleifera influence, which could be a good source to inhibit several major mechanisms involved in cancer development.

• 한국환경과학회지
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• 제13권9호
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• pp.807-817
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• 2004

The modified biochemical oxygen demand (MBOD) were conducted to evaluate the water quality and fertility in the Mangyeong river from november 2002 to april 2003. MBOD method was used to evaluate algal growth potentials and their limiting factors. MBOD depends on the amount of available inorganic nutrient and organic substrate during 5-day incubation in the dark condition at $20^{\circ}C.$ The MBOD assay depends on inorganic nutrients such as phosphorus and nitrogen as well as reduced carbon as called MBOD, MBOD-P, and MBOD-N, respectively. The concentration of pollutants were in the range of 3.08～48.36 mg/L for COD. The concentration of nutrients were in the range of 0.37～111.62 mg/L for dissolved inorganic nitrogen (DIN) and 0.00～1.03 mg/L for dissolved inorganic phosphorus (DIP). The results of MBOD bioassay showed that the MBOD, MBOD-P and MBOD-N values were 15～173 mg $O_2/L,$ 13～165 mg $O_2/L$ and 66～175 mg $O_2/L$ ranges, respectively. The MBOD values are found to be the highest in Iksan River and the lowest in Hari River throughout the Mangyeong River. The relationships of MBOD, MBOD-P and MBOD-N in MBOD method were generally found in MBOD$\risingdotseq$ MBOD-P$\risingdotseq$MBOD-N. But the result of Gosan was appeared to MBOD$\risingdotseq$MBOD-N > MBOD-P. The MBOD-N value was higher 3 to 5 times than the MBOD-P value in the Gosan station. The algal growth potentials expressed as the concentration of chlorophyll-a were maximum 20 times more than algal biomass in the water column.

• 한국관광식음료학회지:관광식음료경영연구
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• 제14권1호
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• pp.59-77
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• 2003

본 연구는 $\beta$-CD처리된 체다 치즈에서의 콜레스테롤 제거 율과 맛의 개발 그리고 쓴 아미노산 물질을 발생을 알아보기 위하여 시행되었다 1) 제어판 (균질화 및 $\beta$-CD처리를 안 함) 2) 우유가공(1000 psi의 우유 균질화 처리, 1% $\beta$-CD처리). 치즈의 콜레스테롤 제거 율은 79.3% 이었다. 짧은 고리의 무지방산(FFA)은 제어된 치즈와 차리 우유를 사용한 치즈 양쪽에서 숙성시간에 따라 증가한다. 5-7개월 숙성시킨 치즈의 짧은 고리 무지방 산의 분해 량은 가공된 묽은 우유로 만든 치즈가 제어된 것보다 더 컸다. 중성의 휘발성 혼합물(neutal volatile compounds)의 생산은 샘플들간에 그리 차이를 보이지 않았다. 쓴맛의 아미노산은 우유 가공된 쪽에서 더 많이 생성되었다. 감각 분석에 의하면 제어된 체다 치즈의 텍스쳐 점수는 상당히 증가하였지만 콜레스테롤이 감소된 치즈에서는 숙성시간에 따라 급격히 감소하였다. 본 연구의 결과는 낮은 온도에서 균질화 시킨 후 $\beta$-CD 처리된 우유로 만들어진 치즈는 효과적인 콜레스테롤 감소와 치즈 숙성이 빠른 반면에. $\beta$-CD 처리에 의한 맛의 저하는 없다는 것을 의미한다.

• 한국지반공학회논문집
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• 제18권2호
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• pp.65-74
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• 2002

지중응력의 측정은 터널 및 지하구조물의 변위와 응력분포를 예측하는데 중요하다. 본 연구에서는 남부 한반도 지역의 지중응력 분포를 음력해방법과 수압파쇄법으로 측정된 새로운 380개의 자료를 통하여 재평가와 이를 고려한 삼차원 터널해석을 실시하였다. 지중응력분포의 결과에 의하면 변성암과 화강암지역의 수평응력분포는 퇴적암과 화산암의 분포에 비하여 불규칙한 경향을 보이고 있다. 화산암 지역에서는 수직응력에 대한 수평음력의 비(측압계수, K)는 150m 깊이 이상 심도에서 1보다 작다. 또한, 국내에서는 처음으로 측정된 지중응력에 대한 삼차원 방향과 크기를 동시에 그림으로 나타내었다. 삼차원 터널 해석에 의하면, 터널 주변의 변위에 대한 방향과 크기는 최대수평음력과 최소수평응력의 차이에 의하여 주로 영향을 받고 있다.

• Parasites, Hosts and Diseases
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• 제50권2호
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• pp.119-126
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• 2012

Carboxylic acids play an important role in both aerobic and anaerobic metabolic pathways of both the snail and the parasite. Monitoring the effects of infection by schistosome on Biomphalaria alexandrina carboxylic acids metabolic profiles represents a promising additional source of information about the state of metabolic system. We separated and quantified pyruvic, fumaric, malic, oxalic, and acetic acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to detect correlations between these acids in both hemolymph and digestive gland gonad complex (DGG's) samples in a total of 300 B. alexandrina snails (150 infected and 150 controls) at different stages of infection. The results showed that the majority of metabolite pairs did not show significant correlations. However, some high correlations were found between the studied acids within the control group but not in other groups. More striking was the existence of reversed correlations between the same acids at different stages of infection. Some possible explanations of the underlying mechanisms were discussed. Ultimately, however, further data are required for resolving the responsible regulatory events. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as disease surveillance and control.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.165-175
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• 2013

The fear that schistosomes will become resistant to praziquantel (PZQ) motivates the search for alternatives to treat schistosomiasis. The antimalarials quinine (QN) and halofantrine (HF) possess moderate antischistosomal properties. The major metabolic pathway of QN and HF is through cytochrome P450 (CYP) 3A4. Accordingly, this study investigates the effects of CYP3A4 inhibitor, ketoconazole (KTZ), on the antischistosomal potential of these quinolines against Schistosoma mansoni infection by evaluating parasitological, histopathological, and biochemical parameters. Mice were classified into 7 groups: uninfected untreated (I), infected untreated (II), infected treated orally with PZQ (1,000 mg/kg) (III), QN (400 mg/kg) (IV), KTZ (10 mg/kg)+QN as group IV (V), HF (400 mg/kg) (VI), and KTZ (as group V)+HF (as group VI) (VII). KTZ plus QN or HF produced more inhibition (P<0.05) in hepatic CYP450 (85.7% and 83.8%) and CYT b5 (75.5% and 73.5%) activities, respectively, than in groups treated with QN or HF alone. This was accompanied with more reduction in female (89.0% and 79.3%), total worms (81.4% and 70.3%), and eggs burden (hepatic; 83.8%, 66.0% and intestinal; 68%, 64.5%), respectively, and encountering the granulomatous reaction to parasite eggs trapped in the liver. QN and HF significantly (P<0.05) elevated malondialdehyde levels when used alone or with KTZ. Meanwhile, KTZ plus QN or HF restored serum levels of ALT, albumin, and reduced hepatic glutathione (KTZ+HF) to their control values. KTZ enhanced the therapeutic antischistosomal potential of QN and HF over each drug alone. Moreover, the effect of KTZ+QN was more evident than KTZ+HF.

• 생태와환경
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• 제46권2호
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• pp.205-214
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• 2013

영산강 하구에서 담수 유입이 부유생태와 수질에 미치는 영향을 파악하기 위해 국가해양환경측정망 장기자료(1999~2008년)인 클로로필 a와 수질 항목들에 대한 계절적, 공간적 변화와 장기변동 추세를 분석하였다. 분석결과, 여름철 (8월) 영산강 방조제 인접정점의 염분은 조사기간 중 가장 낮은 분포를 보였고, 영양염류와 클로로필 a는 전반적으로 높은 분포를 나타냈다. 영산강 담수유입의 지시인자인 표-저층의 염분차 (${\Delta}salinity$)와 영양염류, 클로로필 a는 유의한 양의 상관성을 나타냈으나, 지난 10년동안 담수 방류량은 감소하는 추세를 보였고, 영양염류, 클로로필 a는 전반적으로 증가하는 경향을 나타냈다. 이러한 결과는 영산강 하구 해역 영양염의 공급원이 담수유입뿐만 아니라 인근 지역의 발달에 따른 점오염원 증가 등의 추가적인 요인이 존재할 가능성을 제시한다. 따라서 영산강 하구 주변 해역의 부영양화 방지를 위해서는 영산강 방조제의 방류와 연관한 수질개선방안 마련과 더불어 인근 지역의 점 오염원 파악 및 관리가 병행되어야 할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7541-7546
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• 2015

Aim: To report the histologic findings on Whipple resection specimens and thus determine the extent and spread of carcinomas of ampullary region and head of pancreas in our population. Setting: Section of Histopathology, Department of Pathology, Aga Khan University Hospital (AKUH), Karachi, Pakistan. Materials and Methods: A case series of 311 consecutive Whipple resection specimens received between January 1,2003 and December 31, 2014. Specimens processed for histologic sections and representative sections submitted and histologically examined as per established and standard protocols. All relevant tumor parameters including histologic type, histologic grade, pathologic T and N stage and tumor size were assessed. Epidemiologic data were also recorded. All findings were analysed using SPSS 19.0 software. Results: Ampullary (periampullary) carcinomas were much more common than carcinomas of the head of the pancreas, especially in males, with an average age of 53 years. Mean tumor size was 2.5 cms, over 54% were well differentiated. A large majority were pT2 or pT3 and N0. Carcinomas of pancreatic head were also more common in males, mean age was 55 years, mean tumor size was 3.5cms, and over 65% were moderately differentiated. The majority were T2 or T3 and pN1. Prognostically, significant statistical correlation was seen with tumor grade and pathologic T and N stage (p values statistically significant). However, tumor size was not statistically significant. Conclusions: Ampullary carcinomas are more common compared to pancreatic carcinomas. Majority of ampullary carcinomas were well differentiated while majority of pancreatic carcinomas were moderately differentiated. Large majority of both types of cases were pT2 or T3. Histologic tumor grade and pathologic T and N stage are significantly related to prognosis in Pakistani patients with ampullary and pancreatic cancers.