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Potential Industrial Applications and Evolution of Carbohydrolases and Glycansucrases

  • Kim, Do-Man;Seo, Eun-Seong;Lee, Jin-Ha;Kang, Hee-Kyoung;Cho, Jae-Young
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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    • pp.215-218
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    • 2004
  • Dextrans make up a class of polysaccharides that are D-glucans of various structures with contiguous $\alpha$-1longrightarrow6 ~6 glycosidic linkages in the main chains and $\alpha$-1longrightarrow2, $\alpha$-1longrightarrow3, or $\alpha$-1longrightarrow4 branch glycosidic linkages, depending on the specificity of the particular dextransucrase. Glucansucrases that catalyze glucans synthesis from sucrose. When other carbohydrates, in addition to sucrose, are present in the enzyme digest, the enzyme transfers glucose to the carbohydrate acceptors in the secondary reaction that diverts some of the glucose from incorporation into glucan. Many carbohydrate acceptors have been recognized and the products that result are dependent on the particular enzyme and the structure of the particular acceptor. Because of these unique catalytic characteristics, various dextransucrases have many important industrial and medical uses. To improve the understanding of their action mode and extend their applications, this study describes mechanism of glucan synthesis and potential industrial uses of dextransucrases, and our recent findings on the structural, functional organization and directed evolution of the glucansucrases to offer for designing glucansucrases with improved properties.

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BIOACTIVE PEPTIDES DERIVED FROM FOOD PROTEINS AND PREVENTION OF LIFE-STYLE RELATED DISEASES

  • Yoshikawa Masaaki
    • 한국식품영양과학회:학술대회논문집
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    • 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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    • pp.69-73
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    • 2001
  • Two opioid peptides, YPLDL and YPLDLF, were isolated from enzymatic digests of spinach ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) and named rubiscolin-5 and -6, respectively. These peptides were selective for delta-receptor and the latter was about 3 times more potent than the former. After oral administration in mice at the dose of 100 mg/kg, rubiscolin-6 showed analgesic activity in tail pinch test. It also stimutated learning performance at the same dose in passive avoidance experiment using step-through apparatus. An immunostimulating peptide, MITLAIPVNKPGR, was isolated from a trypsin digest of soybean protein and named soymetide. Immunostimulating activy of soymetide was mediated by fMLP receptor. Interestingly, after oral administration in rats at a dose of 300 mg/kg (po.), soymetide-4 (MITL) protected alopecia (hair-loss) induced by etoposide, a cancer chemotherapy agent. Stimulation of IL-1 release by the peptide was involved in the mechanism. Ovokinin(2-7), RADHPF, is a vasorelaxing peptide released from ovalbumin by the action of chymotrypsin. It lowered blood pressure of spontaneously hypersensive rats (SHR) after oral administration at a dose of 10 mg/kg. RPLKPW, which was designed by replacing 4 amino acid residues in ovokinin(2-7), exhibited hypotensive activity at a dose of 0.1 mg/kg (po.). This peptides was introduced into 3 homologous sites in soybean beta-conglycinin alpha' subunit by site-directed mutagenesis of the cDNA and expressed in E. coli. The minimum effective dose for hypotensive activity of the genetically modified beta-conglycinin alpha' subunit was 10 mg/kg (po.), which is about 1/200 that of ovalbumin.

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체코 큐비즘 가구디자인에 나타난 표현특성에 관한 연구 - 회화, 공예, 건축과의 상호연관성을 중심으로 - (A Study on the Expressive Characteristics of Czech Cubism's furniture Design - chiefly focusing on mutual relationship with Czech Cubism's painting, craft, and architecture)

  • 최병훈;김진우
    • 한국실내디자인학회논문집
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    • 제14권3호
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    • pp.165-172
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    • 2005
  • Cubism is a style that led the way to proclaim a new era of 20th century's art and contemporarily had an influence on several trends of thoughts. Geographically it formed Czech Cubism exerting an effect upon thoughts and plastic art of the progressive art group which showed activity around Prague, Czech in 1911, later for 10 years, it was developed as an unique form of which origin cannot be traced inside and outside Europe and expressed its own plastic art world in craft, furniture, painting, architecture, etc. The object of this study is to pull out the expressive characteristics showed especially in furniture design among Czech Cubism around interrelationship with painting, craft and architecture. The scope of study is to bring out the characteristics about the examples which 7 designers such as Josef Gocar, Pavel Janak, etc., who were representative designers of furniture design of Czech Cubism for 15 years from 1910 to 1925. The method of study is to investigate the origin of Czech Cubism by means of primitive elements of Africa, traces of Islamic architectures, and Czech traditional architectural motive, and the development process of Czech Cubism was arranged around the artist and exhibitions which led this current. After being synthesized the characteristics showed in painting, craft, architecture of Czech Cubism on the basis of the result of this study, the expressive characteristics of furniture design of Czech Cubism were brought out. As a result, the expressive characteristics of furniture design of Czech cubism are indicated in a large way as follow; 1) symbolic characteristic based on primitive plastic art, 2) dynamic characteristic by dividing form, 3) ethnic decorative characteristic combined with national motive. The significance of furniture design of Czech Cubism is not only to accept positively and digest the progressive trend of modern art, that is to say, Paris Cubism but also to succeed in recreate it in its own national style, to play a role to offer another motive to post modern design development at the end of 20th century and by means of these examples to provide the necessity and the base of more profound study in the future.

IoT 보안을 위한 SHA-256 해시 프로세서의 면적 효율적인 설계 (An Area-efficient Design of SHA-256 Hash Processor for IoT Security)

  • 이상현;신경욱
    • 한국정보통신학회논문지
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    • 제22권1호
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    • pp.109-116
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    • 2018
  • 전자서명, 인증 코드, 키 생성 알고리듬 등의 보안 프로토콜에 사용되는 SHA-256 해시 함수를 면적 효율적으로 설계하였다. 설계된 SHA-256 해시 프로세서는 입력 메시지에 대한 패딩 및 파싱 기능을 수행하는 패더 블록을 포함하여 프리프로세싱을 위한 소프트웨어 없이 동작하도록 구현하였다. 라운드 연산을 16-비트 데이터 패스로 구현하여 64 라운드 연산이 128 클록 주기에 처리되도록 하였으며, 이를 통해 저면적 구현과 함께 성능 대비 하드웨어 복잡도 (area per throughput; APT)를 최적화 하였다. 설계된 SHA-256 해시 프로세서는 Virtex5 FPGA로 구현하여 정상 동작함을 확인하였으며, 최대 116 MHz 클록 주파수로 동작하여 337 Mbps의 성능을 갖는 것으로 평가되었다. ASIC 구현을 위해 $0.18-{\mu}m$ CMOS 셀 라이브러리로 합성한 결과, 13,251 GE로 구현되었으며, 최대 동작주파수는 200 MHz로 예측되었다.

흑인 문화의 특수성을 내재한 미국의 캐주얼 패션디자인 특성 (The Characteristics of Black Culture Inherent in the American Casual Fashion Design)

  • 염미선;김영인
    • 복식
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    • 제63권6호
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    • pp.14-28
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    • 2013
  • This study aims to identify the elements of black culture and use it to analyze the formative characteristics of casual fashion items as well as the symbolic meanings presented in the graphic designs of the items. First of all, in order to distinguish the identity of black culture, this study made a division into racial, social, and artistic aspects based on cultural specificities of Stuart Hall, a culture theorist. This was for a theoretical review and based on this review, a theoretical framework was developed to analyze the characteristics of American casual fashion design of black people. The case study selected 1719 fashion images of 137 male brands and 33 female brands from websites of companies manufacturing casual wear of black people in America. The following is a summary of the study results. The conceptual characteristics based on specificities of black culture can be categorized into ten attributes: playfulness in order to digest human grief and emotion positively, satire of the mainstream society, spontaneity through emotional expression from the heart, abstractness of various emotions elating suffering, humor trying to express negative aspects as the meaning of light and innocent laughter, dependence with passive attitudes toward discrimination and master-slave relationships in history, resistance against discrimination and disadvantages, African orientation and primitiveness pursuing natural conditions, aggressiveness against violence and unfair treatment, and confidence and defiance against social deprivation and corruption. Based on the results of an analysis through formative classification system founded on racial, social, and artistic aspects of black people, specificities of black culture are of formative characteristics of their casual fashion design. These elements are presented on graphic t-shirts through strong colors, loose silhouette, and details with many pockets. These characteristics were reflected in symbolic meanings presented in the graphic.

Screening of Bacterial Leaf Blight Resistance Genes (xa5, xa13, Xa21) using Sequence Tagged Site (STS) Marker in Korean Varieties and Landraces

  • Kim, Young-Chang;Park, Yong-Jin;Ma, Kyung-Ho;Lee, Jung-Ro;Kim, Chang-Young;Choi, Jae-Eul;Kang, Hee-Kyoung
    • Plant Resources
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    • 제7권3호
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    • pp.187-194
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    • 2004
  • Sequence-tagged site (STS) markers tightly linked to the bacterial leaf blight (BLB) resistance genes, xa5, xa13 and Xa21, were used in this study. A survey was conducted to find polymorphisms between the resistant and susceptible germplasm in rice. 500 of Korean varieties and 100 of landraces were evaluated in this study. STS marker, RG207 was used to having xa5 resistance gene of rice germplasm. 27 varieties of Korean germplasm showed resistant for xa5 gene. The RG136 an xa-13 marker resulted in a single band of approximately 1kb in all the rice accessions studied. In order to detect polymorphism, digestion of the polymerase chain reaction (PCR) product was performed using a restriction enzyme Hinf Ⅰ. The resistant lines resulted in two bands 0.5kb on digestion with Hinf Ⅰ, while the same enzyme did not digest the PCR product of susceptible lines. No polymorphism was detected in Korean varieties and landraces, indicating that they probably do not contain xa13 gene. pTA248 an Xa-21 marker detected a band of 1kb in the resistant lines and bands of either 750bp or 700bp in the susceptible lines. Among germplasm tested, there are no varieties and landraces with Xa21 resistant gene. The results of the germplasm survey will be useful for the selection of parents in breeding programs aimed at transferring these bacterial blight resistance genes from one varietal background to another.

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117명 두드러기 환자의 특성에 대한 사상체질적 임상분석 (A Clinical Analysis on 117 Patients with Urticaria Based on Sasang Constitutional Medicine)

  • 김지환;박성식
    • 사상체질의학회지
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    • 제26권3호
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    • pp.304-317
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    • 2014
  • Objectives The purpose of this study was to find out whether there were differences of urticaria's disease aspect between patients classified into Sasang Constitution Types(SCTs) and whether those differences could be associated with Ordinary symptoms. Methods Medical records and questionnaires about 117 patients who visited one Korean medicine hospital due to urticaria were collated and statistically analyzed. Results 1) Ages 20 to 30, women, Soeumin(SE) and patients in conditions of chronic urticaria over 6 weeks were the majority among 117 patients in this study. Food and stress were most chosen as the main cause of urticaria. 2) Soyangin(SY) showed more severe symptoms of urticaria than other SCTs. In particular, the severity of pruritus, distribution of lesions and vulnerability to stress was statistically significant compared to other SCTs(p<0.05). SE expressed urticaria's symptoms at the medium-level of SY's and Taeeumin(TE)'s. TE exhibited relatively weak symptoms but TE only had slightly higher number of patients with angioedema compared to other SCTs. Taeyangin(TY) was only one case so more researches are needed. 3) In dispositional symptoms, SY had low quality of sleep and defecation. SE could not digest oily food well, felt dizzy and cold well, and had cold hands and feet. TE could eat and sweat much and tended to snore well. Conclusions In this study, urticaria had common cause of both stress and food in all SCTs but onset and severity of symptoms were different between SCTs. It could interpreted that these differences between SCTs were associated with ordinary symptoms native to each SCT.

American Women's Adoption of Pants and the Changing Definition of Femininity during World War II

  • Lee, Yhe-Young;Farrell-Beck, Jane
    • International Journal of Human Ecology
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    • 제11권1호
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    • pp.23-33
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    • 2010
  • Articles from The New York Times and magazines including Consumer Digest, Journal of Home Economics, Scholastic, Time and Woman's Home Companion were analyzed in this study and focused on the following research questions: How did the social situation influence American women's adoption of pants during World War II? How were the social opinions of women's adoption of pants? How did American women's adoption of pants and the social opinions on women's pants represent the process of change in the definition of femininity during World War II? Women were encouraged to wear pants in work places because many women had to work in defense industries and farms. Women had to wear pants during the winter to keep warm in order to conserve oil, rubber, and other materials. In addition, wearing men's clothes became a fashion trend among college women during this period. However, practicality was often not the primary thing alone to consider in women's fashion. Femininity was still important in women's fashion. There were criticisms over the women's adoption of pants. Regulations against pants were imposed on women, while there were women who wanted to dress like ladies even at defense industries. An abrupt change in women's gender roles and the increased adoption of trousers aroused social ambivalence about the traditional definition of femininity. Even though many women returned to their homes after the war, the social demand of practicality in women's day-time clothes during the war offered women the experience of comfort and practicality in pants. These experiences contributed to paving the way for more women to adopt pants and helped establishing a new definition of femininity after the war.

MD5 및 HAS-160 해쉬 알고리즘을 통합한 면적 효율적인 설계에 관한 연구 (A Study on Area-Efficient Design of Unified MD5 and HAS-160 Hash Algorithms)

  • 손승일
    • 한국정보통신학회논문지
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    • 제16권5호
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    • pp.1015-1022
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    • 2012
  • 본 논문에서는 MD5 및 HAS-160 해쉬 알고리즘을 통합한 하드웨어 설계에 대해 다룬다. MD5와 HAS-160 해쉬 알고리즘은 임의의 길이를 갖는 메시지를 512비트의 메시지 블록 단위로 처리하여 고정된 길이의 해쉬 코드를 출력한다. MD5의 해쉬 코드는 128비트이며, HAS-160 해쉬 코드는 160비트이다. 설계된 통합 해쉬 코어는 HAS-160코어와 비교하여 32%의 슬라이스를 추가적으로 사용하지만, 고정된 메시지 버퍼 공간만을 사용한다. 단계당 1클럭에 수행되는 통합 해쉬 코어는 92MHz에서 동작하며, MD5 모드에서는 724Mbps로 HAS-160 모드에서는 581Mbps의 속도로 메시지를 다이제스트(Digest)하는 성능을 갖는다. 본 논문의 통합 해쉬 코어는 전자상거래, 데이터 무결성, 디지털 서명 등의 분야에서 응용이 가능할 것으로 사료된다.

Rhizopus oryzae에 의한 생전분 분해에 관한 연구 -분리균주에 의한 효소 생산 조건 및 에탄올 발효- (Studies on Digestion of Raw Starch by Rhizopus oryzae - Optimum Condition of Enzyme Production and Ethanol Fermentation -)

  • 김찬조;오만진;이종수
    • 한국미생물·생명공학회지
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    • 제13권4호
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    • pp.329-337
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    • 1985
  • 토양등으로부터 생전분 분해효소 생산능이 있는 409주의 곰팡이와 62주의 세균 및 2주의 방선균을 분리하고 이들 중에서 7-BU 균주를 선정하여 동정하고 밀기울을 기본배지로 한 효소 생산조건 및 생전분을 이용한 알코올 발효 실험 등을 실시하여 다음과 같은 결과를 얻었다. 1. 생전분 분해효소 생산능이 강하다고 인정된 7-BU 균주는 Rhizopus oryzae로 동정되었다 2. 7-BU 균주의 효소생산 최적온도는 3$0^{\circ}C$이고 pH는 4.0이었으며 배양 72시간 후에 최고의 역가를 보였고 밀기울 배지에 옥수수전분을 0.5% 첨가하였을 때 효소생산이 더욱 촉진되었다. 3. 조효소액에 의한 각종 생전분의 가수분해율은 반응 4일에 쌀전분 89.2%, 옥수수전분 85%, 고구마전분 82%, 감자전분 36%이었고 쌀가루는 86%, 옥수수가루 81%. 고구마가루 79%, 감자가루 30%이었다. 4. 옥수수가루, 쌀가루, 고구마가루와 감자가루를 원료로 하여 조효소액과 효모를 첨가한 후 알코올 발효 실험을 한 결과 담금 4일 후 쌀가루구 9.4%, 옥수수가루구 9.0%, 고구마가루구 8. 1%, 감자가루구 5.4%의 에탄을 생성을 보였다.

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