• 제목/요약/키워드: DIG

검색결과 139건 처리시간 0.021초

'역사'의 기억과 해체 그리고 재구성: 수잔-로리 팍스의 "미국 극" (Memory, deconstruction and reconstruction of 'history': Suzan-Lori Parks' The America Play)

  • 박진숙
    • 영어어문교육
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    • 제15권3호
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    • pp.315-332
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    • 2009
  • The purpose of this paper is to scrutinize how Parks recalling, deconstructing and reconstructing African-American memories of the absences in American history through a black Lincoln impersonator named, The Foundling Father or The Lesser Known. Parks unearths and reconstitutes a significance for the historical event of Lincoln's assassination by repetitive mimicry and verbal puns. As a pun of the Founding Father, the Foundling Father reminds us of Abraham Lincoln, one of the most venerated figures in American history. In the first act, the black Foundling Father performs as The Great Man. This inverted minstrel show of the black Foundling Father performing a white Lincoln exposes the desire of the Foundling Father to insert his narrative within the history of America. With a series of assassinations, the African-American performers figuratively murder the power and control of the American myth. In the second act, his wife Lucy and his son Brazil dig relics from the past out of 'The great hole of history' instead of the Foundling Father. Digging and burial for African-Americans are their livelihood and their calling as well. As Parks pointed out, they should locate the ancestral buried ground, dig for bones and find bones because so much of African-American history has been unrecorded, dismembered and washed out. Parks leaves the possibilities of digging and burying on the black history through Lucy and Brazil.

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Chirp 방식을 이용한 40kbps급 협대역 전력선 통신 모뎀 구현에 관한 연구 (A Study on the Implementation of a 40kbps Narrowband Powerline Communication Modem Using the Chirp Technique)

  • 박성욱;엄우용
    • 대한전자공학회논문지TE
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    • 제42권4호
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    • pp.53-62
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    • 2005
  • 대역 확산 기술의 하나인 Chirp 방식을 이용하여 기존의 전력선 통신기술 보다 고속 전송이 가능한 40kbps급 협대역 전력선 통신 모뎀을 구현하였다. 본 논문에서 구현한 모뎀은 CEBus 규격을 사용하였으며 100-380kHz의 선형가변 주파수 신호가 25us 동안 지속한 심벌을 사용하였다. 실험결과 구현된 모뎀은 무부하 선로, 각종 부하 및 잡음환경에서의 실험을 하였다. 실험 결과, Chirp 방식의 통신 기술이 협대역에서 40kbps 이상의 속도를 낼 수 있는 가능성을 확인하였다.

Digoxigenin-labeled in situ Hybridization을 이용한 오제스키병의 진단 (Digoxigenin-labeled in situ Hybridization for Diagnosis of Aujeszky's disease in Pigs)

  • 김순복;문운경;송근석;여상건
    • 한국수의병리학회지
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    • 제1권2호
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    • pp.119-124
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    • 1997
  • The objective of this study was to develop digoxigenin (DIG)-labeled in situ hybridization (ISH) test for diagnosis of Aujeszky's Disease(AD) in infected organs. Specific DNA with well conserved gene sequences encoding gp50 antigen in AD virus (ADV) was obtained by Polymerase Chain Reaction (PCR) method. A pair of oligonucleotide primers used in PCR allowed amplification of a 217 bp sequence from the gp50 ADV gene. The DNA was then labeled with DIG by primer labeling method for use as probe in ISH test to detect ADV nucleic acids in various tissue. Positive hybridization was demonstrated by dark pigmentation in nuclei and cytoplasm of ADV infected cells particularly in brain tonsillar crypt epithelium and pulmonary alveolar cells. This result suggests that ISH is a valuable sensitive and rapid diagnostic test for AD.

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지중압입체를 이용한 지하구조물 축조방법의 적용성 연구 (A Study on application of Trapezoidal Steel Box Tunnelling Method)

  • 전승배
    • 한국재난정보학회 논문집
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    • 제4권2호
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    • pp.138-154
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    • 2008
  • The conventional non-dig underground structure building method which made an appearance to reduce the social and environmental costs and maximize the efficiency of the social overhead capital facilities could not help being uneconomical because of many problems such as unnecessary excessive excavation, water leakage, obstacle interference, difficulty of curvilinear application and connection complexity between propelled and injected bodies due to indiscriminate application of small and large circular steel pipes without consideration of the site conditions. The T.S.T.M, in which a protruded square tube is applied as a propulsion and injection body in a design that considered site conditions such as ground condition, depth of soil and live load, was able to be economical as it solved the problems of water resistance, minimization of obstacle interference and curvilinearity, and we can see that it can be applied to all grounds by utilizing or complementing the target ground in terms of engineering. Also in configuring the transverse section, it is possible to not only secure excellent structural safety but also implement all of the above engineering characteristics not only in the square cross section but also in the arch cross section, so it was possible to build structures on any section or ground, and we could confirm the LCC reduction effect and the VE effect.

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융합 센서 네트워크를 위한 데이터 기반 색인 (A Data Driven Index for Convergence Sensor Networks)

  • 박정석
    • 한국융합학회논문지
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    • 제7권6호
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    • pp.43-48
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    • 2016
  • 무선센서 네트워크는 센서 데이터베이스 관리 시스템을 통해 보다 효율적으로 개발 및 운용될 수 있다. 센서 데이터베이스 관리 시스템은 무선센서 입력에 대해 선언된 사용자 정의 질의를 처리하기 위해 사용자들에게 익숙한 SQL 유형의 사용자 접속을 지원한다. 무선센서 네트워크상의 전형적 질의 유형은 임의의 스냅 샷 값 검색이나 오래도록 지속되는 연속 질의 형태를 갖는다. 무선센서 네트워크상에서 질의 처리는 베이스스테이션으로부터 여러 노드들로 질의를 보내는 과정과 여러 노드에서 얻어지는 질의 결과를 베이스스테이션으로 회수하는 과정이 있는데 이러한 질의의 파급이나 베이스스테이션으로의 결과 전송은 많은 에너지 소모를 요구한다. 이 논문은 무선센서 네트워크상에서 영역 질의를 처리함에 있어 질의 및 결과를 파급시키는데 소모되는 에너지를 절약시켜 주기 위한 분산정보수집(DIG: Distributed Information Gathering)이라고 이름붙인 효율적 색인 방법을 제안한다.

미꾸라지 성장 호르몬 염기 서열의 특성에 대하여 (Characterization of growth hormone-like sequence of loach, Misgurnus mizolepis)

  • 김진경;송영환
    • 한국어병학회지
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    • 제7권2호
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    • pp.95-103
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    • 1994
  • 미꾸라지의 성장호르몬 유전자를 분리하기 위하여 미꾸라지의 cDNA library를 준비하였다. total RNA는 미꾸라지의 뇌하수체로부터 얻었으며 oligo (dT)-coupled magnetic bead를 이용하여 total RNA로부터 mRNA를 순수분리하였다. 정제된 mRNA는 cDNA를 합성하기 위한 기질로 사용하였으며, 합성된 cDNA는 EcoRV/Smal으로 절단된 pBlueKS+ plasmid vector에 삽입하였다. 모든 ligation 반응용액을 E. coli, JM109 균주에 형질전환을 유도하였으며 형질전환 효율을 최대화시키기 위하여 전기천공법을 이용하였다. 얻어진 모든 형질전환주들을 DIG로 표지된 Tilapia의 성장호르몬 유전자를 이용하여 고밀도 colony hybridization 에 의하여 검색하였다. 양성반응을 나타내는 10개의 형질전환주를 분리하여 2차 colony hybridization 및 southern hybridization에 의하여 성장호르몬 유전자가 cloning 되었음을 확인하였다. 10 개의 형질전환주 중 하나인 pCGH1을 probe로 사용한 Tilapia 성장 호르몬 유전자의 염기서열과 비교분석하였으며 53.2%의 유사성을 나타냄을 확인하였다.

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Development of DNA probe for a protistan parasite of tunicate Halocynthia roretzi

  • Choi, Dong-Lim;Hwang, Jee-Youn;Choi, Hee-Jung;Hur, Young-Baek
    • 한국어병학회지
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    • 제23권3호
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    • pp.313-322
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    • 2010
  • Edible tunicate Halocynthia roretzi, one of the most commercially important aquatic organisms in Korea, has been killed by tunic softness syndrome since last decade. The intracellular protistan parasite observed by the transmission electron microscope in hemocytes of the tunicate was considered to be the causative agent of the mass mortality. The goal of the present work is to examine the characteristic features of the parasite by identifying the 18S rDNA sequences of the parasite. The experiments conducted include amplification of presumptive 18S rDNA from diseased tunicate tissues with UNonMet-PCR and sequencing the product. A preliminary phylogenetic analysis was performed on the presumptive parasite rDNA. A digoxigenin labeled DNA probe was designed on the basis of the sequences of rDNA. Dig-ISH assay was conducted to diagnose the protistan parasite. A PCR using UNonMet-PCR primer generated 595 bp SSU rDNA fragment. Subsequently, PCRs with primer pair expended this sequence to 1542 bp. This is the first partial sequences of SSU rDNA gene to be published on the protistan parasite that has presumed causing the mass mortality of tunicate. Since the Dig-ISH technique demonstrated the presence of infection in hemocytes on the all host tissues, the fragment was confirmed to be the intracellular protistan parasite SSU rDNA. A phylogenetic analysis suggested that the protistan parasite may be a unique eukaryote that is closely related to Apicomplexa.

Advanced Tools for Modeling, Design and Optimization of Wind Turbine Systems

  • Iov Florin;Hansen Anca Daniela;Jauch Clemens;Sorensen Poul;Blaabjerg Frede
    • Journal of Power Electronics
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    • 제5권2호
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    • pp.83-98
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    • 2005
  • As wind turbine technology and control has advanced over the last decade, this has led to a high penetration of wind turbines into the power system. Whether it be for a large wind turbine or an offshore wind farm with hundreds of MW power capacity, the electrical system has become more and more important in controlling the interaction between the mechanical system of the wind turbine and the main power system. The presence of power electronics in wind turbines improves their controllability with respect not only to its mechanical loads but also to its power quality. This paper presents an overview of a developed simulation platform for the modeling, design and optimization of wind turbines. The ability to simulate the dynamic behavior of wind turbines and the wind turbine grid interaction using four simulation tools (Matlab, Saber, DIgSILENT and HAWC) is investigated, improved and extended.

Revisiting Social Discount Rates for Public Investment

  • SONG, JOONHYUK
    • KDI Journal of Economic Policy
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    • 제39권2호
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    • pp.75-98
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    • 2017
  • This paper aims to estimate the social discount rate (SDR) rather than dig into its theoretical foundation. As SDRs can be derived by investigating both the rate of return on investment and the social time preference rate, we estimate the marginal productivity of both private and public capital and the time preference rate based on the Euler equation. In order to provide a single representative SDR, the weighted averages of the marginal productivity and time preference rate, whose weights are determined by the flow of funds data reflecting the social demand of funds, are presented. Based on the empirical results, we argue that the marginal productivity of private capital stands in the middle of the 3% range while that of public capital varies from 4.5% to 8.6%, with the time preference rate showing a decreasing trend from 3.2% in the early 2000s to 1.2% by around 2030. The single representative SDR or the weighted SDR is estimated to be approximately 3.0~4.5% and expected to continue its downward trend for the foreseeable future.