• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.101-109
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• 2000

Duck viral hepatitis is an acute, highly infectious viral disease of young dacklings aged from two days to three weeks. The significant lesion associated with the disease was enlarged liver including necrotic foci and numerous hemorrhagic spots. We have isolated five strains of duck hepatitis virus (DHV) from field cases showing about 20% mortality with a sign of opisthotonos. When a-day-old ducklings were intramuscularly inoculated with one of the isolates, 92% of the birds were died within 5 days. We attempted to develop an attenuated strain of duck hepatitis virus (DHV) using one of the isolates by serial chicken embryo passages. The propagation of DHV in chicken embryos was carried 140 passages. The virus titer increased gradually from the $21^{st}$ through the $50^{th}$ passage, but there was no significant increase of virus titer in subsequent passages after then. Through the serial passages, the virulence of the virus for chicken embryos was gradually increased but decreased for ducklings. The pathogenicity of the virus for ducklings was preserved up to the $21^{st}$ passage but disappeared at the $50^{th}$passage. An attenuated Korean isolate which was passaged 140 times in chicken embryos gave good protection in ducklings against both challenge infection to a Korean virulent strain and to a DHV-DRL strain, a type 1 reference strain of DHV, which indicated that the Korean isolates could be classified as DHV type 1. And the above results suggest that an attenuated Korean isolate can be used for developing a live DHV vaccine.

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.110-116
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• 2000

Duck viral hepatitis is an acutic, highly infectious viral disease of young ducklings. The most practical means for controlling duck viral hepatitis is the vaccination of ducklings or of a breeding stock. We attempted to develop a vaccine strain of duck hepatitis virus (DHV) using a Korean isolate by serial chicken embryo passages. The propagation of DHV in chicken embryos was carried 140 passages. After the $50^{th}$ passage, of which the virus was non-pathogenic for ducklings, approximately every $20^{th}$ passage of the virus was tested for vaccinal efficacy. Both the $70^{th}$ and $90^{th}$ passage of the virus gave good protection against challenge infection to a DHV-DRL reference strain(type 1) and a virulent Korean isolate. The $110^{th}$, $125^{th}$ and $140^{th}$ passage of the virus were less protective than the $70^{th}$ and $90^{th}$ passage, which means that more than $110^{th}$ passage may lead to over-attenuation of the virus. Ducklings vaccinated with the chicken-embryo-adapted virus by oral, intramuscular or eye drop administration showed earlier resistance to challenge infection from 3 to 7 days postvaccination. Of the above methods, ducklings vaccinated intramuscularly presented the most rapid resistance against challenge. The minimum immune dose of the chicken-embryo-adapted virus in ducklings was also studied. Ducklings inoculated with a dose of $10^{2.0}\;ELD_{50}$ and below were not fully protected against challenge with a virulent DHV, showing a protection rate of 67% to 73%, but ducklings inoculated with a dose of $10^{3.0}\;ELD_{50}$ and over were completely protected. The virus yield of the chicken-embryo-adapted DHV was examined at 24hrs and 48hrs of the incubation time in the allantoic fluid, embryo head and embryo minus head of the embryonating egg. In all three components, the titer of the virus was higher at 48 hours than that at 24 hours after incubation. And the titer of the virus was higher in the embryo minus head, embryo head and the allantoic fluid, in order. Field trials for evaluating the efficacy of the attenuated DHV as a live vaccine were done in duck farms with about 25% mortality of flocks resulting from duck viral hepatitis. After the use of the experimental vaccine, the mortality due to duck viral hepatitis was dramatically reduced in the farms. These results indicated that the attenuated DHV using a Korean isolate could be a good candidate as a live vaccine strain of DHV in Korea.

• Journal of Korean Society of Transportation
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• v.25 no.6
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• pp.79-88
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• 2007

Design Hourly Volume (DHV) is the hourly volume used for designing a section of road. DHV is also used to estimate the expected number of vehicles to pass or traverse the relevant section of road in a future target year. The Design Hour Factor (DHF) is defined as the ratio of DHV to Average Annual Daily Traffic (AADT). In addition to high precision of predicted traffic volume, in order to design a roadway to be the proper scale, applying appropriate DHFs considering traffic flow characteristics and type of area which surrounds the relevant roadway is important. This study categorizes sections of expressway (Suh Hae An Expressway) according to their area type and estimates DHFs utilizing traffic data obtained from a vehicle detection system (VDS). This study shows that DHFs calculated using VDS data are different from those using traffic data acquired from a coverage survey. While AADTs from both data show similar values, peak hour volumes from both data show significant differences especially for recreational areas. DHFs from the coverage survey are quite different from the values provided by the Korean design guide or previous research results and DHFs for urban areas are higher than recreational areas. However, DHFs from VDS shows similar values to previous research results. The result of this study suggests that using VDS for estimating DHFs is more reliable than using a coverage survey.

• Korean Journal of Poultry Science
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• v.38 no.1
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• pp.13-19
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• 2011

In January 2010, high mortalities of ducklings occurred in two adjacent farms. Ducklings in farm A showed neurologic signs including paddling, opisthotonus and lameness, but ducklings from farm B had no specific clinical signs. At post mortem, diffused hemorrhagic spots in the liver and hemorrhages in the small intestine were observed in ducklings from farm A, and ducklings from farm B had only proventricular ulceration. Microscopically, multiple necrosis of hepatocytes, hyperplasia of the bile ducts, hemorrhages, infiltration of lymphocytes and bacterial colonies were commonly observed in the liver of ducklings from both farms. Also, type A and C duck hepatitis virus (DHV) were isolated from farm A and farm B, respectively and Salmonella typhimurium was commonly isolated and identified serologically and biochemically. To our knowledge, this is the first report about the co-infection of DHV and Salmonella typhimurium in ducklings, and co-circulation of type A and C duck hepatitis viruses in Korea.

• 한국ITS학회:학술대회논문집
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• v.2006 no.10
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• pp.1-6
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• 2006

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.13 no.5
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• pp.1-10
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• 2014

This research focuses on the warrants for the Two-Way Left-Turn Lanes (TWLTL). Using a microscopic traffic simulation tool, two key parameters were investigated herewith. One is a wide range of the Design Hourly Volume (DHV), reflective of recent Korean roadway volume characteristics, that is conventionally reduced from the Average Daily Traffic (ADT). The other is driveway spacing, the length of the middle-lane section where two conflicting left-turn demands often compete for space. In addition, unlike previous researches, the way and the procedure the TWLTL operation is realized in the VISSIM S/W with its add-on application such as VISVAP is clearly stated and described in detail. According to the result of simulations for 10 volume scenarios, as expected, the higher the volume level is, the more delay the left-tuner experience. The Level Of Service (LOS) for most cases was in the range of C and D based on the non-signalized intersection LOS criteria. Furthermore, the TWLTL was found operable up to the volume level of 1,116 and 1,860 vph in heavy direction (equivalent of volume level 7) for 3-lane and 5-lane facility respectively, which covers significant portion of existing two to four-lane highway volumes in Korea.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.12 no.2
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• pp.52-62
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• 2013

Design Hourly Factor(DHF) is defined as the ratio of design hourly volume(DHV) to Average Annual Daily Traffic(AADT). Generally DHV used the 30th rank hourly volume. But this case DHV is affected by holiday volumes so the road is at risk for overdesigning. Computing K factor is available for counting 8,760 hour traffic volume, but it is impossible except permanent traffic counts. This study applied three method to make DHF, using 30th rank hourly volume to make DHF(method 1), using peak hour volume to make DHF(method 2). Another way to make DHF, rank hourly volumes ordered descending connect a curve smoothly to find the point which changes drastic(method 3). That point is design hour, thus design hourly factor is able to be computed. In addition road classified 3 type for national highway using factor analysis and cluster analysis, so we can analyze the characteristic of DHF by road type. DHF which was used method 1 is the largest at any other method. There is no difference in DHF by road type at method 2. This result shows for this reason because peak hour is hard to describe the characteristic of hourly volume change. DHF which was used method 3 is similar to HCM except recreation road but 118th rank hourly volume is appropriate.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.3 no.1 s.4
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• pp.45-52
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• 2004

There are many cities installing ITS(Intelligent Transportation Systems) and running TMC(Trafnc Management Center) to improve mobility and safety of roadway transportation by providing roadway information to drivers. There are many devices in ITS which collect real-time traffic data. We can obtain many valuable traffic data from the devices. But it's impossible to avoid missing traffic data for many reasons such as roadway condition, adversary weather, communication shutdown and problems of the devices itself. We couldn't do any secondary process such as travel time forecasting and other transportation related research due to the missing data. If we use the traffic data to produce AADT and DHV, essential data in roadway planning and design, We might get skewed data that could make big loss. Therefore, He study have explored some imputation techniques such as heuristic methods, regression model, EM algorithm and time-series analysis for the missing traffic volume data using some evaluating indices such as MAPE, RMSE, and Inequality coefficient. We could get the best result from time-series model generating 5.0$\%$, 0.03 and 110 as MAPE, Inequality coefficient and RMSE, respectively. Other techniques produce a little different results, but the results were very encouraging.

• Journal of the Korean Society of Radiology
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• v.15 no.5
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• pp.723-730
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• 2021

This study tries to compare dose distribution between arc radiation therapy and Tomotherapy, which are main radiation therapy modalities. The subjects of this study are lung cancer patients. For planning target volume (PTV), a dose of 60.0 Gy was set as a basis. The PTVmean of Arc was 61.04 Gy, and that of Tomotherapy was 58.50 Gy. The total lung capacities of Arc and Tomotherapy were 3.0 Gy and 4.24 Gy, respectively. The mean heart doses of Arc and Tomotherapy were 0.13 and 0.34, respectively; the mean trachea dose of Arc and Tomotherapy were 1.35 and 2.58, respectively; the mean esophagus dose of Arc and Tomotherapy were 0.41 and 0.86, respectively; the mean spinal cord dose of Arc and Tomotherapy were 3.65 and 4.68, respectively. With regard to the appropriateness of therapeutic effect in DHV, both modalities seemed appropriate. Tomotherapy protected normal tissues better than Arc radiation therapy. In Tomotherapy, patients need to have treatment long in a limited space. If such a point is overcome, Tomotherapy is better. Otherwise, Arc radiation therapy can be applied. This study was conducted with treatment planning images. Therefore, the results of this study are different from actual treatment results. If more research is conducted to overcome the limitation, the effects of radiation therapy are expected to increase further.

• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.239-241
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• 2000

From 5 forest soil samples, the isolation frequency of oligotrophic Actinomycetes was investigated with nutrient and minimal media. The frequency of soil Actinomycetes by the minimal media. The frequency of soil Actinomycetes by the minimal media of DHV, WA, BA and NA was similar to the value by HV nutrient medium. In addition, different Actinomycetes were isolated from all the media used in this experiment and then their growth were tested on nutrient (Bennett's agar) and minimal media(WA). In consequent, the number of different Actinomycetes from minimal media was 26.1 strains, whereas the number of from nutrient medium (HV) was 26.6 strains. Furthermore, the percentage of facultative and obligate strains among the oligotrophic Actinomycetes was 90% and 10%, respectively.