• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.6
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• pp.275-282
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• 2011

Current service discovery protocols such as UPnP, Jini, SLP provide the basic function which is message exchange pattern, service representation and description, in service discovery. They does not guarantee service interoperability among service discovery. Therefore, in this paper, we design and implement CLAM (Component-based Autonomic Layered Middleware) agent platform to enable an efficient service discovery through extension of DF agent function in FIPA-compliant specification. Also, we propose an efficient service discovery mechanism using DHT-Chord algorithm to guarantee scalability and interoperability in DF agent.

• Microbiology and Biotechnology Letters
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• v.20 no.5
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• pp.498-504
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• 1992

In order to investigate the antimutagenicity of the sweet potato enzymatic browning reaction products (SPEBRP) were studied the DNA breaking action, spore rec assay and Ames test. In the DNA breaking action of reaction mixture of SPEBRP and polyphenol compounds with an agarose horizonal electrophoresis, catechol (CAT)-SPEBRP and hydroxyhydroquinone (HHQ)SPEBRP inhibited DNA breaking effect in the presence of $Fe^{2+}$. In the spore ree assay using Bacillus subtilis H17(rec+) and M45(rec-), 3,4-dihydroxytoluene (DHT)-SPEBRP showed strong antimutagenic effects on MNNG. In the Ames test using Salmonella tYPhimurium TA 98 and TA 100, pyrogallol(PYR)-, 3,4-dihydroxytoluene (DHT)- and hydroxyhydroquinone (HHQ)-SPEBRPs suppressed about 67%, 71% and 63% in the mutagenesis induced by Benzo($\alpha$)Pyrene(B($\alpha$)p).

• Bulletin of the Korean Chemical Society
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• v.31 no.10
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• pp.2897-2902
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• 2010

Seven fully optimized geometries of 3,6-dihydrazino-1,2,4,5-tetrazine (DHT) dimers have been obtained with density functional theory (DFT) method at the B3LYP/$6-311++G^{**}$ level. The intermolecular interaction energy was calculated with zero point energy (ZPE) correction and basis set superposition error (BSSE) correction. The greatest corrected intermolecular interaction energy of the dimers is $-23.69\;kJ{\cdot}mol^{-1}$. Natural bond orbital (NBO) analysis is performed to reveal the origin of the interaction. Based on the vibrational analysis, the changes of thermodynamic properties from the monomers to dimer with the temperature ranging from 200.0 K to 800.0 K have been obtained using the statistical thermodynamic method. It was found that the hydrogen bonds dominantly contribute to the dimers, while the binding energies are not only determined by hydrogen bonding. The dimerization process can not occur spontaneously at given temperatures.

• Biomolecules & Therapeutics
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• v.29 no.6
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• pp.667-677
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• 2021

The elevated expression of the hyaluronan-mediated motility receptor (HMMR) is known to be highly associated with tumor progression in prostate cancer, but the molecular mechanisms underlying the regulation of HMMR expression remain unclear. Here, we report that mammalian target of rapamycin (mTOR) is a key regulator of HMMR expression, for which its kinase activity is required. Pharmacological inhibitors of mTOR, such as rapamycin and Torin2, markedly suppressed the mRNA level as well as the protein level of HMMR in LNCaP and PC-3 cells. Our data demonstrate that such regulation occurs at the transcription level. HMMR promoter reporter assays revealed that the transcription factor SRF is responsible for the mTOR-mediated transcriptional regulation of HMMR gene. Consistently, the suppression of HMMR expression by Torin2 was noticeably reversed by the overexpression of SRF. Moreover, our findings suggest that the SRF binding sites responsible for the transcriptional regulation of HMMR through the mTOR-SRF axis are located in HMMR promoter sequences carrying the first intron, downstream of the translational start site. Furthermore, the upregulation of HMMR by DHT was abolished by stimulation with rapamycin, prior to DHT treatment, suggesting that mTOR activity is required for the induction of HMMR expression by androgen. Collectively, our study provides new mechanistic insights into the role of mTOR/SRF/AR signaling in HMMR regulation in prostate cancer cells.

• Journal of Medicine and Life Science
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• v.16 no.1
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• pp.34-38
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• 2019

Hypogonadism is a clinical syndrome that results in hormone deficiency and can be classified as 1) primary caused by the gonadal failure and 2) secondary by the hypothalamus-pituitary gland dysfunction and/or cardiometabolic complications. Recently the presence of thyroid hormone receptors in different testicular cell types was demonstrated, and thus thyroid dysfunctions would be another cause of secondary hypogonadism. Thus, we investigated the effects of perinatal hypothyroidism on hypogonadism in male Sprague-Dawley rats. Perinatal hypothyroidism was induced by daily administration of 0.05% 6-propyl-2-thiouracil (PTU) by tap water from gestation day 15, which were compared with negative control (PTU (-)) group. At postnatal day 28, hypothyroid pups were divided into 2 groups: PTU (+) group - continued PTU treatment and PTU (+/-) group - stopped PTU until postnatal day 49. Body weights, dehydrotesosterone (DHT), and testosterone levels were checked 2 and 3 weeks after grouping. Body weights were significantly decreased in PTU(+) and PTU(+/-) groups compared with PTU (-) group at postnatal day 28. 3 weeks later, PTU (+/-) group significantly gained weight compared with PTU (+) group. DHT and testosterone levels significantly decreased with PTU treatment, but increased 3 weeks after stopping PTU administration. Perinatal PTU-induced hypothyroid hypogonadism was sustained for 2 weeks after stopping PTU administration, but restored gonadal hormone levels 3 weeks after stopping PTU. These results suggest that researchers should design an experiment on hypothyroid hypogonadism based on the estimated period.

• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.553-559
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• 2019

To investigate the effects of antioxidant activities and hair loss prevention of extracts from Platycodon grandiflorum, we've prepared chloroform (CF) and ethylacetate fractions (EA) extracted from P. grandiflorum. In the results of DPPH radical scavenging assay, the two fractions showed dose-dependent antioxidant activities. Furthermore, in the ABTS assay, the two fractions exhibited the inhibitory effect over 90% at 10, 50, 100, 200 mg/mL. To investigate the inflammation inhibitory effect, we used RAW264.7 cells, these extracts were inhibited inflammatory reaction by suppressing the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in dose-dependent manner. In the assay of HaCaT cells' proliferation, the 24 hr treatment of the extracts significantly accelerarted cell proliferation in the range of concentrations used. The two fractions inhibited the proliferation of Malssezia furfur, the bacteria induce the dandruff. Finally, the CF could significantly inhibit the DHT production at 1, 10, 50, $100{\mu}g/mL$, but EA showed the inhibitory effect at the concentration over $50{\mu}g/mL$. The overall results of this study suggest that the chloroform (CF) and ethylacetate fractions (EA) from P. grandiflorum could be a useful raw material for the hair loss prevention products.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.1285-1287
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• 2009

VoIP 통신에서 단말간의 세션을 수립하고 종료하는 역할을 하는 SIP는 기본적으로 서버-클라이언트 방식으로 이루어져있다. 하지만 이는 확장성과 설치비용, 단일실패 지점 측면에서 큰 단점을 갖는다. 최근에 DHT를 이용한 구조적인 P2P를 SIP와 결합하여 위의 단점들을 극복하기 위한 연구들이 활발히 진행되고 있다. 하지만 DHT를 이용한 방식은 기존 서버-클라이언트 방식에 비해 검색지연이 늘어난다는 큰 단점을 가지고 있다. 검색지연은 SIP에서 QoS의 중요한 지표중 하나인 호 설정 지연에 60~80% 정도의 큰 영향을 미치게 되므로 해결해야할 중요한 문제이다. P2PSIP에서는 종단 노드가 검색한 사용자의 주소를 캐싱하여 같은 사용자를 재 검색시에 사용하고 있다. 이는 네트워크 전체 오버헤드 감소에 큰 영향을 주지 못할뿐만 아니라 발생된 검색비용을 자신만이 사용할 수 있으므로 비효율적이다. 이에 본 논문에서는 도메인으로 구분된 계층적 P2PSIP네트워크에서 프록시 서버의 역할을 하는 슈퍼노드를 이용한 효과적인 캐싱 방법을 이용하여 호 설정 지연을 단축할 수 있는 방안을 제안하다.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.750-753
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• 2009

최근 유비쿼터스 및 모바일 환경에서 Peer-to-Peer(P2P)시스템에 대한 연구들이 활발히 진행되고 있다. 모바일 환경에서는 모바일 환경의 제약사항과 함께 이동성이라는 특징이 중요하게 고려된다. 노드의 이동에 의해서 네트워크의 주소가 변경되어 P2P 네트워크 망에서 유효하지 않은 접속 정보들이 생기고 이러한 정보들이 검색 및 P2P 시스템의 전체적인 성능을 떨어뜨리게 된다. 이 논문은 이러한 접속 정보들의 불일치를 해결하여 접속 정보를 유지하기 위한 효과적인 방법론인 이동전파 알고리즘을 제안한다. 이 알고리즘은 접속 정보의 불일치를 해결하기 위해 정보를 빠르게 처리해서 P2P 시스템의 성능을 향상하는 것을 목표로 한다. DHT 기반 P2P 시스템을 기반으로 하여 제안하는 알고리즘을 적용하여 실험을 하였으며, 그 결과로 처음 신규 발견된 정보를 재 발견하는 경우에 성능이 기존의 DHT 기반의 P2P 시스템보다 80% 향상되었음을 알 수 있다.

• Nutrition Research and Practice
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• v.11 no.5
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• pp.373-380
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• 2017

BACKGROUND/OBJECTIVES: This study was conducted to investigate the effect of a corn silk extract on improving benign prostatic hyperplasia (BPH). MATERIALS/METHODS: The experimental animals, 6-week-old male Wistar rats, were divided into sham-operated control (Sham) and experimental groups. The experimental group, which underwent orchiectomy and received subcutaneous injection of 10 mg/kg of testosterone propionate to induce BPH, was divided into a Testo Only group that received only testosterone, a Testo+Fina group that received testosterone and 5 mg/kg finasteride, a Testo+CSE10 group that received testosterone and 10 mg/kg of corn silk extract, and a Testo+CSE100 group that received testosterone and 100 mg/kg of corn silk extract. Prostate weight and concentrations of dihydrotestosterone (DHT), $5{\alpha}$- reductase $2(5{\alpha}-R2)$, and prostate specific antigen (PSA) in serum or prostate tissue were determined. The mRNA expressions of $(5{\alpha}-R2)$ and proliferating cell nuclear antigen (PCNA) in prostate tissue were also measured. RESULTS: Compared to the Sham group, prostate weight was significantly higher in the Testo Only group and decreased significantly in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05), results that were consistent with those for serum DHT concentrations. The concentrations of $(5{\alpha}-R2)$ in serum and prostate as well as the mRNA expression of $(5{\alpha}-R2)$ in prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups than that in the Testo Only group (P < 0.05). Similarly, the concentrations of PSA in serum and prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05) than in the Testo Only group. The mRNA expression of PCNA in prostate dose-independently decreased in the Testo+CSE-treated groups (P < 0.05). CONCLUSIONS: BPH was induced through injection of testosterone, and corn silk extract treatment improved BPH symptoms by inhibiting the mRNA expression of $(5{\alpha}-R2)$ and decreasing the amount of $(5{\alpha}-R2)$, DHT, and PSA in serum and prostate tissue.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.5
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• pp.750-760
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• 2009

In this study, the various effects of Salicornia herbacea, including antioxidative function, antibiotic, and anti-inflammatory effect, and the effect of sebiparous reduction due to intervention of the DHT hormone were investigated using. The result of this study Based on this analysis, the serum total cholesterol, triglyceride, and WBC were found to be significantly decreased and SOD, Ca, K, and Zn were shown to be significantly increased significantly in the group that received the SH pill group after trial fora 12 week trials. Testosterone and DHT were increased in the SH pill group, but it was not statistically significant. In the case of the skin condition measurements, the number of blackhead pores, skin oiliness, keratin and, MIC were significantly decreased, and the pH was decreased to normal pH in the SH pill group. Therefore, it was confirmed that overall skin condition was improved due to Salicornia herbacea diet. The results of this research result can contribute so demonstrate the potential of actively using that the Salicornia herbacea can be actively developed as a health supplement, and a medication.