• YAKHAK HOEJI
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• v.46 no.3
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• pp.208-212
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• 2002

Dehydroevodiamine HCl (DHED), which is a component separated from Evodia rutaecarpa Bentham, has novel anticholinesterase and antiamnesic activities in the scopolamine-induced amnesia model. Several studies suggest that DHED might be an effective drug for the Alzheimer's disease and the vascular type of dementia. In order to evaluate the mutagenic potential of DHED, Salmonella typhimurium reversion assay, chromosomal aberration test on Chinese hamster lung cells, in vivo micronucleus assay using mouse bone marrow cells, and comet assay were performed. DHED did not increase the number of revertant in the reverse mutation test using Salmonella typhimurium TA1535, TA1537, TA98, TA100. DHED HCl, at concentration of 5 and 10 $\mu\textrm{g}$/mι, increased the number of chromosome aberrated Chinese hamster lung cells with 5 and 10％, respectively. In mouse micronucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocyte was observed in ICR mice orally administered with DHED. DHED was tested for ability to induce genotoxic effect in L5178Y cells (mouse lymphoma cells) using the single cell gel electrophoresis assay (comet assay). In comet assay, tail moment did not increase in L5178Y cells treated with 10, 100, 300 $\mu$M DHED.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.1
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• pp.55-64
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• 2017

Progressive memory impairment such as that associated with depression, stroke, and Alzheimer's disease (AD) can interfere with daily life. In particular, AD, which is a progressive neurodegenerative disorder, prominently features a memory and learning impairment that is related to changes in acetylcholine and abnormal ${\beta}$-amyloid ($A{\beta}$) deposition in the brain. In the present study, we investigated the effects of dehydroevodiamine HCl (DHED) on cognitive improvement and the related mechanism in memory-impaired rat models, namely, a scopolamine-induced amnesia model and a $A{\beta}_{1-42}$-infused model. The cognitive effects of DHED were measured using a water maze test and a passive avoidance test in the memory-impaired rat models. The results demonstrate that DHED (10 mg/kg, p.o.) and Donepezil (1 mg/kg, p.o.) ameliorated the spatial memory impairment in the scopolamine-induced amnestic rats. Moreover, DHED significantly improved learning and memory in the $A{\beta}_{1-42}$-infused rat model. Furthermore, the mechanism of these behavioral effects of DHED was investigated using a cell viability assay, reactive oxygen species (ROS) measurement, and intracellular calcium measurement in primary cortical neurons. DHED reduced neurotoxicity and the production of $A{\beta}$-induced ROS in primary cortical neurons. In addition, similar to the effect of MK801, DHED decreased intracellular calcium levels in primary cortical neurons. Our results suggest that DHED has strong protective effects against cognitive impairments through its antioxidant activity and inhibition of neurotoxicity and intracellular calcium. Thus, DHED may be an important therapeutic agent for memory-impaired symptoms.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.1
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• pp.55-59
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• 2014

Dehydroevodiamine HCl (DHED) has been reported to prevent memory impairment and neuronal cell loss in a rat model with cognitive disturbance. We investigated the effect of DHED on memory impairment and behavioral abnormality caused by stress. We demonstrated that DHED can improve stress-induced memory impairments and depression-like behaviors by using open-field test, Y-maze test and forced swimming test. DHED treatment significantly recovered the decreases in the levels of neural cell adhesion molecule (NCAM) proteins caused by stress and the decreases in cell viability. Our results suggested that DHED is a potential drug candidate for neuronal death, memory impairment and depression induced by stress.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.280-286
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• 2012

Clean energy farming is the agricultural activity to improve an efficiency of agricultural energy use and to replace fossil fuels. This study was carried out to establish the clean energy farming system in the controlled cultivation of vegetable crop (cucumber) adopting the biogas production facility. In order to design the clean energy farming system, mass and energy balance was analyzed between the controlled cultivation system and the biogas production facility. Net yearly heating energy demands ($E_{YHED}$) of forcing and semi-forcing cultivation types were 48,697 and $13.536Mcal\;10^{-1}$ in the controlled cultivation of vegetable cucumber. To cover these $E_{YHED}$, the pig slurry of 511 and $142m^3\;10a^{-1}$ (biogas volume of 9,482 and $2,636Nm^3\;10a^{-1}$, respectively, as 60% methane content) were needed in forcing and semi-forcing cultivation types. The pig slurry of $511m^3\;10a^{-1}$ caused N 1,788, $P_2O_5$ $511kg\;10a^{-1}$ in the forcing cultivation type, and the pig slurry of $142m^3\;10a^{-1}$ caused N 497, $P_2O_5$ $142kg\;10a^{-1}$ in the semi-forcing cultivation type. The daily heating energy demand ($E_{i,DHED}$) by the time scale analysis showed the minimum $E_{i,DHED}$ of $7.7Mcal\;10a^{-1}\;day^{-1}$, the maximum $E_{i,DHED}$ of $515.1Mcal\;10a^{-1}\;day^{-1}$, and the mean $E_{i,DHED}$ of 310.2 in the forcing cultivation type. And the minimum $E_{i,DHED}$, the maximum $E_{i,DHED}$, and the mean $E_{i,DHED}$ were 5.3, 258.0, and $165.1Mcal\;10a^{-1}\;day^{-1}$ in the semi-forcing cultivation type, respectively. Input scale of biogas production facility designed from the mean $E_{i,DHED}$ were 3.3 and $1.7m^3\;day^{-1}$ in the forcing and the semi-forcing cultivation type. The maximum $E_{i,DHED}$ gave the input scale of 5.4 and $2.7m^3\;day^{-1}$ in the forcing and the semi-forcing cultivation type.

• YAKHAK HOEJI
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• v.46 no.6
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• pp.452-458
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• 2002

Dehydroevodiamine-HCl (DHED), which is a component separated from Evodia rutaecarpa Bentham, has novel anticholinesterase and antiamnesic activities in a scopolamine-induced amnesia model. Several studies suggest that DHED might be an effective drug for Alzheimer's disease and a vascular type of dementia. DHED was at dose levels of 0, 50, 100 and 200 mg/kg/day administered intraperitoneally to Sprague-Dawley male rats for 60 days before mating and to females from 14 days before mating to 7 days after mating. Effects of the DHED on general symptom and reproductive performance of parent animals and embryonic development were examined. In male parents, whereas no death was observed, reduction in the increase rate of body weight was found at 200 mg/kg. In female parents, both of the mating performance and the fertility of parent animals were decreased at 200 mg/kg, but not significantly. In 200 mg/kg treated group, the fetal death rate was increased but total fetuses showed no changes compared to the control group. There were no malformed F1 fetuses in all groups.

• The Korean Journal of Physiology and Pharmacology
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• v.8 no.1
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• pp.65-67
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• 2004

Dehydroevodiamine (DHED) is one of the bioactive components of the Chinese herbal medicine Wu-chu-yu-tang that has been shown to produce various pharmacological effects. In the present study, we investigated the pharmacokinetics of DHED after intravenous administration of two doses (2.5 and 5 mg/kg) in anesthetized rats. The plasma concentration of DHED was measured by reverse-phase high-performance liquid chromatography with UV detection. The mean area under the curve of the time-concentration profile was $21.9\;and\;53.9\;{\mu}g{\cdot}min/ml$ after the 2.5- and 5-mg/kg doses, respectively, and the volume of distribution was 1584.9 and 1580.6 ml following 2.5- and 5-mg/kg doses, respectively. Plasma concentration profiles versus time were compatible with a two-compartment model and first-order kinetics. The terminal elimination half-life was $91.8{\pm}16.6\;min$ and $78.7{\pm}11.9\;min$ in the dose of 2.5 and 5 mg/kg, respectively. This is the first report to study the pharmacokinetics of DHED in animals.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.4
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• pp.656-664
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• 2006

In this paper, we introduce a new LUT based watermarking method for a halftone image. Watermark bits are hidden at pseudo-random locations of halftone image in the proposed method. The pixel values of the halftone image are determined from the LUT entry indexed by both the neighborhood halftone pixels and current grayscale value. The LUT is trained by a set of grayscale images and corresponding halftone images. Advantage of the LUT method is that it can be executed very fast compared with other watermarking methods for a halftone image. Therefore, the algorithm can be embedded in a printer. Experiments for real scanned images showed that the method is a feasible method to hide the large amount of data within a halftone image without noticeable distortion and comparing to the DHED method, is almost same in quality but significantly shorten in processing time.