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Study on Mutagenicity of DehydroevodiamineㆍHCl(DHED)  

성이숙 (중앙대학교 약학대학)
정성윤 (중앙대학교 약학대학)
정주연 (중앙대학교 약학대학)
채규영 (중앙대학교 약학대학)
진미령 (중앙대학교 약학대학)
최봉웅 (제일약품㈜)
장병모 (제일약품㈜)
김대경 (중앙대학교 약학대학)
Publication Information
YAKHAK HOEJI / v.46, no.3, 2002 , pp. 208-212 More about this Journal
Abstract
Dehydroevodiamine HCl (DHED), which is a component separated from Evodia rutaecarpa Bentham, has novel anticholinesterase and antiamnesic activities in the scopolamine-induced amnesia model. Several studies suggest that DHED might be an effective drug for the Alzheimer's disease and the vascular type of dementia. In order to evaluate the mutagenic potential of DHED, Salmonella typhimurium reversion assay, chromosomal aberration test on Chinese hamster lung cells, in vivo micronucleus assay using mouse bone marrow cells, and comet assay were performed. DHED did not increase the number of revertant in the reverse mutation test using Salmonella typhimurium TA1535, TA1537, TA98, TA100. DHED HCl, at concentration of 5 and 10 $\mu\textrm{g}$/mι, increased the number of chromosome aberrated Chinese hamster lung cells with 5 and 10％, respectively. In mouse micronucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocyte was observed in ICR mice orally administered with DHED. DHED was tested for ability to induce genotoxic effect in L5178Y cells (mouse lymphoma cells) using the single cell gel electrophoresis assay (comet assay). In comet assay, tail moment did not increase in L5178Y cells treated with 10, 100, 300 $\mu$M DHED.
Keywords
Salmonella typhimurium reversion assay; chromosomal aberration test; micronucleus assay; Comet assay; DHED;
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