• Title/Summary/Keyword: DH

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A design of ABC(Advanced Block Cipher) Algorithm (ABC(Advanced Block Cipher) 알고리즘 설계)

  • Lee, Byung-Kwan;Jeong, Eun-Hee;Yun, Dong-Sic
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.3 no.2
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    • pp.64-69
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    • 2010
  • This paper designs the ABC(Advanced Block Cipher) algorithm which is a 64byte block encryption algorithm, improves the performance of encryption process time, and makes an key exchange using EC-DH. The ABC algorithm reduces basic memory occupation rates using the original data position exchange method which is a data swap key without S-Box, IP-Box and etc. Also, it prepares the exposure of symmetric key using the unfixed encryption(decryption) key excepting the fixed encryption(decryption) key. Therefore, the proposed ABC algorithm in this paper is a proper encryption algorithm in lower memory environment and mobile banking.

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Characterization of Complemented Mutants in Pseudomonas fluorescens and Cloning of the DNA Region Related in Antibiotic Biosynthesis (길항세균 Pseudomonas fluorescens의 Complemented Mutant에 대한 특성조사에 및 길항물질 유전자 Cloning)

  • Kim, Young;Cho, Yong-Sup
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.151-156
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    • 1994
  • Pseudomonas fluorescens produces the antibiotic, 2,4-diacetylphloroglucinol (Phl), which promotes plant growth by inhibiting bacteria and fungi. Cosmids (genomic library) were mobilized into Phl-nonproducing mutants through the triparental matings with pRK2013 as the helper plasmid at the frequency of 8.37$\times$10-4. Complemented mutants that showed antibiotic activity were selected among about 2,000 transconjugants. The complemented mutants were confirmed by acquired drug resistances (kanamycin and tetracycline). The antibiotic substances of wild type and complemented mutants showed the most excellent anti-bacterial activity. Inhibitory effects of complemented P. fluorescens against phytopathogenic fungi were equal to the parental strain. Complemented mutant and wild type of P. fluorescens were causal microbes of fungal morphological abnormalities. Complemented mutants in potato dextrose agar supplemented with bromothymol blue also showed restoration of glucose utilization as wild type. Plasmids of complemented mutants were isolated from transconjugant sand transformed into competent cells of E. coli DH5$\alpha$. The plamid DNA was reisolated from transformed E. coli DH5$\alpha$.

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Evaluation of Optimal Culture Conditions for Recombinant Ghost Bacteria Vaccine Production with the Antigen of Streptococcus iniae GAPDH

  • Ra, Chae-Hun;Kim, Yeong-Jin;Park, So-Jin;Jeong, Chang-Wha;Nam, Yoon-Kwon;Kim, Ki-Hong;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.19 no.9
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    • pp.982-986
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    • 2009
  • For the production of ghost bacteria vaccine to prevent the streptococcal disease in aquaculture fish species, a double cassettes vector was constructed and cloned in Escherichia coli DH5${\alpha}$. Ghost bacteria vaccine production from Escherichia coli DH5${\alpha}$/pHCE-InaN-GAPDH-Ghost 37 SDM (SIG) was maximized at a glucose concentration of 1 g/l, agitation of 300 rpm, and aeration of 1 vvm. The maximal efficiency of ghost bacteria formation was obtained at the mid-exponential phase ($OD_{600}=2.0$) with the concentration of 0.77 g/l for SIG. The molecular mass of GAPDH was detected at 67 kDa with the insoluble fraction, by SDS-PAGE and Western blot. The protective efficacy of ghost bacteria vaccine was evaluated by challenge test using olive flounder. The cumulative mortalities of the positive control, formalin-killed cell (FKC) vaccine, and SIG vaccine immunized groups were 91%, 74%, and 57%, respectively. These results suggest that SIG vaccine showed efficacy as a vaccine and had a higher potential to induce protective antibodies than did FKC vaccine.

The Future Past of Humanities Research: Musing Methodology in the Digital Convergence Era

  • Kim, Jiyun
    • International journal of advanced smart convergence
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    • v.9 no.3
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    • pp.161-168
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    • 2020
  • Over the last half-century, computer science has revolutionarily changed the landscape of humanities research. This digital shift in research methodology has reached from the brainstorming process to preserving, constructing, collecting, visualizing, and even analyzing materials. Such transformation has brought about the birth of the new field of study: Digital Humanities (DH). DH undeniably has saved much of the physical chores and provided a new angle to interpret the text, thereby making its meteoric rise as a promising future of the humanities. Based on such innovation, electronic circuitry can seem to replace the imagination that detects relationships and significances of research data with ever-improving interfaces. However, despite hitherto technological development, the thousands-year-old essence of traditional liberal arts-human creativity-remains the heart of humanities research and always will. This paper starts by proving this proposition in the way of comparing the old and new liberal arts research methods, focusing on literary studies. Meanwhile, it thoroughly investigates how digitalized bibliographies, search engines, databases, and digital projects provide the most useful data preservation and virtual experience of browsing in the library, along with their limitations due to the intrinsic quality of humanities research data. Also, it probes the differences between traditional and digital data analysis in current methods of literary studies, ultimately presenting the ideal direction for humanities development in the era of digital convergence.

Corrosion Behaviour of DH36 Steel Used for Oil Platform in Splash Zones

  • Liu, J.G.;Li, Y.T.;Hou, B.R.
    • Corrosion Science and Technology
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    • v.14 no.4
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    • pp.190-194
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    • 2015
  • The splash zone is the most corrosive area of the marine environment, and the corrosion of steel structures exposed in this area is a serious concern. DH36 steel is one of most commonly used steels for offshore oil platforms in China, and its corrosion behaviour in splash zones was studied in this paper. Polarization curves were obtained from the corroded steel exposed in this area while the morphologies and rusts of the rust steel were characterized using scanning electron microscopy and X-ray diffraction. Double rust layers were formed in the splash zone. The inner layer contained magnetite and fine flaky lepidocrocite, and the outer layer was composed of accumulated flaky lepidocrocite and a small amount of goethite. In the wet period, the iron dissolved and reacted with lepidocrocite, and magnetite appeared, while the magnetite was oxidized to lepidocrocite again during the dry period. Electrochemical reduction and chemical oxidization cycled in intermittent wetting and drying periods, and magnetite and lepidocrocite were involved in the reduction reaction, leading to serious corrosion.

Fatigue properties of welded joints for TMCP steels (TMCP 고장력강 용접부의 피로 특성에 관한 연구)

  • 임채범;권영각;엄기원
    • Journal of Welding and Joining
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    • v.8 no.2
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    • pp.40-52
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    • 1990
  • Fatigue behavior of the AH, DH and EH grade TMCP(Thermo-Mechanical Control Process) steels was studied. High cycle and low cycle fatigue tests were carried out for the weldment and base metal of each steel. The results showed that the fatigue limit at 2 * $10^6$ cycles was 33 to 37 kg/$mm^2$ for the base metal and 30 to 34 kg/$mm^2$ for the weldment. The ratio of fatigue limit to tensile strength for TMCP steels was 0.65 to 0.71, which was a value close to the upper limit for the ordinary steels. It was also found that the high cycle fatigue behavior of TMCP steels could be affected by the microstructures of base metal. It will be necessary to have fine structure for TMCP steels to increase the fatigue resistance. In low cycle fatigue test, the fatigue lifetime of AH and DH steels accorded well with the ASME best fit curve, while that of EH steel was considerably lower than the fatigue lifetime of the other steels. Fatigue resistance of the weldment made by high heat input(180kJ/cm) welding was not lower than that made by low heat input(80kJ/cm) welding in case of high cycle fatigue, but the high heat input welding decreased the fatigue resistance in case of low cycle fatigue.

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Estimation of Human Carcinogenic Potency (HCP) of Carcinogens in Risk Assessment and Management. (위해성 평가 및 관리에 있어서 발암물질의 인체발암능력 평가)

  • 이병무;김대영;김세기;김근종
    • Environmental Mutagens and Carcinogens
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    • v.19 no.1
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    • pp.39-45
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    • 1999
  • Human Carcinogenic Potency (HCP) can be estimated based on human daily exposure dose to carcinogen (Dh), body weight (Wh), 10% tumorigenic dose (TD10), and slope factor at TD10 (Q10) from 2-yr bioassay data. This approach is more relevant to humans generally exposed to low doses of carcinogens and can reduce more of extrapolation errors from high dose in animal experiments to low dose in humans than HERP (human exposure dose/rodent potency dose) proposed by Ames et al. (Science, 236, 271-280, 1987). TD50 and HERP have been routinely used to compare rodent carcinogenic potency and human carcinogenic potency, but those approaches have had limitations in extrapolation of high dose to low dose in humans. The advantages of HCP are to estimate human exposure dose (Dh) by human monitoring instead of environmental monitoring, to consider slope factor (Q10) which reflects the tendency of curve at low dose, and to use TD10 which represents much lower dose thant TD50 or HERP. HCP will be a useful parameter for the estimation of human carcinogenic potency in risk assessment and management of carcinogens.

결정질 실리콘 태양전지 모듈 규격평가와 가속평가, 그리고 표준화의 역사

  • Baek, Do-Hyeon
    • Electrical & Electronic Materials
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    • v.27 no.10
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    • pp.11-21
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    • 2014
  • 1975년 이후로 태양전지 평가 실험실과 제조업체들이 직접적으로 많은 노력을 하여 태양전지 모듈에 대한 가속 스트레스 평가를 개발하고 평가하였다. 이 노력들은 이미 알려진 불량 메커니즘을 토대로 모듈 디자인의 민감한 취약 부분을 발견하기 위한 평가를 목적으로 평가 순서/방법 표준화에 많은 기여를 하였다. 이러한 평가 순서/방법은 국가와 국제 모듈 규격 표준화에 적용되었다. 그리고, 실제 외부에서 설치되어 한 번도 문제가 발생되지 않은 평가들은 중간에 제거 되기도 하였다. 가장 대표적인 예로 twist test는 모듈 설치 후 발생되는 불량에 대한 평가이지만, 실제로 불량은 한번도 발견되지 않았다. 덧붙여서, 표준 평가들은 많은 특화된 가속평가를 사용하게 되는데, 이는 문제점들을 조사하거나 특정 모듈 디자인에 대한 잠재적인 불량을 조사하기 위함이다. 실제 수명예측을 위한 가속평가는 가속 인자의 지식이 반드시 필요하지만, 태양전지 분야에서는 문헌이 많이 부족하다. 많은 연구자들이 85/85 DH 평가시간과 습한 지역에서 수년 간 사용된 모듈과의 상관관계를 만들기 위해 시도 하였다. 그리고 이 모델은 DH 평가 표준시간인 1,000시간으로 정의하는데 많은 영향을 미치기도 했다. 하지만, 이 모델은 단지 단일 모드 불량일 경우에만 적용이 되는 것이다. 다른 불량에 대한 가속인자들은 여전히 잘 알려져 있지 않다. 따라서, 모듈이 비록 표준 규격시험을 통과했다고 하더라도 제품이 특정 기간 동안 전력을 생산할 수 있다고 말할 수 없다. 또한, 이 표준평가가 모든 불량 메커니즘에 대한 평가가 되지 않기 때문에 제품의 수명을 결정하는데 사용될 수 없다.

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The Experimental Model Development of Antibiotic Resistance Gene Transfer Characteristics with Various Micropollutants (미량오염물질에 의한 항생제 내성 유전자 전이 특성에 대한 실험모델 개발)

  • Kim, Doocheol;Oh, Junsik;Kim, Sungpyo
    • Journal of Korean Society on Water Environment
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    • v.28 no.6
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    • pp.911-916
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    • 2012
  • Recently, antibiotic resistant genes (ARGs) in the environment are emerging as pollutants, since these genetic contaminants can eventually be transferred to human pathogens. The aim of this study was to develop the experimental model of antibiotic resistant gene (ARG) plasmid transfer as a function of various environmental conditions. For this purpose, the multi drug resistant plasmid pB10, which is known to be originally isolated from a wastewater treatment plant, was selected as a model transfer plasmid and Escherichia coli $DH5{\alpha}$ containing pB10 was used as a model donor. Pseudomonas aeruginosa, an opportunistic pathogen, was selected as the recipient for the conjugation experiment. When the donor and recipient were exposed to various stressors including antibiotics and heavy metal as a function of the concentrations (10, 100 and, 1000 ppb), statistically increased plasmid transfer rate was observed at a concentration of 10 ppb of tetracycline and sulfamethoxazole compared to control (no antibiotic exposure). Accordingly, the developed experimental ARG model by various stressor is a promising tool for evaluating the dissemination of ARGs by micro-contaminants in aquatic environment.

Fatigue Crack Growth Behavior of Austenite Stainless Steel in PWR Water Conditions (모사원전환경에서 오스테나이트 스테인리스강의 피로균열성장 평가)

  • Min, Ki-Deuk;Lee, Bong-Sang;Kim, Seon-Jin
    • Korean Journal of Materials Research
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    • v.25 no.4
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    • pp.183-190
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    • 2015
  • Fatigue crack growth rate tests were conducted as a function of temperature, dissolved hydrogen (DH) level, and frequency in a simulated PWR environment. Fatigue crack growth rates increased slightly with increasing temperature in air. However, the fatigue crack growth rate did not change with increasing temperature in PWR water conditions. The DH levels did not affect the measured crack growth rate under the given test conditions. At $316^{\circ}C$, oxides were observed on the fatigue crack surface, where the size of the oxide particles was about $0.2{\mu}m$ at 5 ppb. Fatigue crack growth rate increased slightly with decreasing frequency within the frequency range of 0.1 Hz and 10 Hz in PWR water conditions; however, crack growth rate increased considerably at 0.01 Hz. The decrease of the fatigue crack growth rate in PWR water condition is attributed to crack closure resulting from the formation of oxides near the crack tips at a rather fast loading frequency of 10 Hz.